LU500683B1 - Purpose of Composition Comprising Black Hulless Barley and Black Quinoa in Preparation of Anti-oxidation Products - Google Patents

Abstract

The disclosure claims a purpose of a composition comprising black hulless barley and black quinoa in the preparation of anti-oxidation products, wherein the composition comprises 3 - 24 parts of alcohol extract of black hulless barley, 2 - 16 parts of aqueous extract of black hulless barley, 4 -10 parts of alcohol extract of black quinoa and 5 - 7 parts of aqueous extract of black quinoa. The composition according to the disclosure has high bioactivity and antioxidant activity.

Description

Purpose of Composition Comprising Black Hulless Barley and Black Quinoa 7500689 in Preparation of Anti-oxidation Products

TECHNICAL FIELD The disclosure relates to the field of medicine, specifically relating to a purpose of a composition comprising black hulless barley and black quinoa in the preparation of anti-oxidation products.

BACKGROUND With the improvement of living standards, people pay increasing attention to skin problems caused by oxidation, such as skin aging, inflammation and even cancer. Direct contact of human skin with oxygen causes skin aging and damage which are important features of senility. Senility is caused by the accumulation of reactive oxygen radicals or failure of scavenging reactive oxygen radicals, and excess radicals in vivo cause cell damage and pigmentation. Compared with the radicals produced by the body during metabolism, oxygen radicals are the most harmful to the body. Oxygen radicals include superoxide anion radicals, hydroxyl radicals, hydrogen peroxide and singlet oxygen which are normal metabolites of the body. Although they are continuously produced in metabolism, they are not harmful under normal circumstances as there is a defense system in the body. Superoxide anion radicals can be eliminated by superoxide dismutase (SOD). SOD is an important oxygen radical scavenger in vivo and can balance oxygen radicals in vivo, avoiding adverse reactions caused by a high concentration of superoxide anion radicals in vivo. Although the human body has its own antioxidant enzymes and non-enzymatic systems against oxidative damage, the protective systems cannot remove all reactive oxygen species (ROS) if the human body is damaged by excessive ROSs. Therefore, it is necessary to use antioxidants. However, long-term use of antioxidants can have toxic effects. Therefore, people are increasingly seeking more natural and safer antioxidants and anti-aging drugs. Hordeum vulgare ssp. vulgare, also known as hulless barley, is a crop grown in a low-temperature and low-oxygen environment on the plateau. It belongs to Hordeum Linn. in the family Gramineae. It has been used as the main food crop for thousands of years in many countries and regions in the world. Hulless barley includes white hulless 7500689 barley, black hulless barley and dark green hulless barley, and black hulless barley is a unique variety in Tibet. Comai County in the Shannan Prefecture at the north foot of the Himalayas is rich in black hulless barley because of its special climate and soil. Black hulless barley is rich in high fat, crude fiber and anthocyanin and has a good healthcare function, so it is favored by food processors and markets.

Chenopodium quinoa willd, also known as South American Quinoa, quinoa, golden millet, etc., is originated in Andes Mountains of America and is the main traditional food of the Inca. Chenopodium quinoa willd with unique, rich and comprehensive nutritional value has nurtured the Inca. It is known as the mother of food, and can also replace infant milk powder. It is rich in protein, dietary fiber and minerals, with balanced amino acid composition, and it is also rich in phenolic acids, polyphenols, flavonoids and other phenolic compounds. Phenolic compounds ingested in the human body have physiological effects such as antioxidation, anti-inflammation, antiviral effects, and anticancer. Black quinoa belongs to the family Bataceae and is neutral in nature and sweet in taste. Black quinoa is rich in protein, carbohydrates, fat, amino acids (including all nine essential amino acids for the human body) with proper proportion and is easy to be absorbed. It is also rich in unsaturated fatty acids, anthocyanins, flavonoids, B-glucan, choline, betaine, a-linolenic acid, various mineral elements, vitamin B, folic acid, vitamin E and other compounds. The content of dietary fiber is as high as 7.1%, and black quinoa does not contain cholesterol. Black quinoa has the effects of repairing physique, improving adaptability, regulating endocrine, preventing diseases, maintaining beauty and keeping young, losing weight, relaxing bowels, delaying aging, preventing cancer, lowering blood pressure, blood lipid and blood glucose, preventing cardiovascular and cerebrovascular diseases, and assisting in treating chronic diseases.

SUMMARY A main objective of the disclosure is to provide a purpose of a composition comprising black hulless barley and black quince in the preparation of anti-oxidation products. The composition has high bioactivity to improve the content of superoxide dismutase (SOD) and reduce the content of malondialdehyde (MDA), and has 500685 antioxidant activity.

To realize the above objective, the disclosure provides the following technical solutions: A purpose of a composition in the preparation of anti-oxidation products, wherein the composition comprises 3 - 24 parts of alcohol extract of black hulless barley, 2 - 16 parts of aqueous extract of black hulless barley, 3 -10 parts of alcohol extract of black quinoa and 5 - 7 parts of aqueous extract of black quinoa.

According to a preferred embodiment of the disclosure, the mass ratio of the alcohol extract and aqueous extract of black hulless barley is 1.5-2.4:1 (preferably

1.5:1), and the mass ratio of the alcohol extract and aqueous extract of black quinoa is

0.8~2:1 (preferably 0.8:1).

According to a preferred embodiment of the disclosure, the composition comprises 12-24 parts of alcohol extract of black hulless barley, 8-16 parts of aqueous extract of black hulless barley, 4-7 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa.

Further, the composition comprises 12 parts of alcohol extract of black hulless barley, 8 parts of aqueous extract of black hulless barley, 4 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa.

According to a preferred embodiment of the disclosure, the alcohol extract may be one or a combination of methanol, ethanol, butanol or pentanol.

Further, the alcohol extract is ethanol, specifically, 20~100% v/v ethanol-water solution, and 35~95% v/v ethanol-water solution is preferred.

According to a preferred embodiment of the disclosure, the product is a superoxide dismutase agonist and/or a product for reducing malondialdehyde content in vivo.

The MDA content is an important parameter reflecting the antioxidant potential of the body. It can reflect the rate and intensity of lipid peroxidation and indirectly reflect the degree of tissue peroxidation damage.

Further, the product is a product for scavenging oxygen radicals in vivo, and the product can be food, health care products, medicines or cosmetics. 700088 The disclosure also provides an anti-oxidation product, wherein the product is prepared by using alcohol extract of black hulless barley, aqueous extract of black hulless barley, alcohol extract of black quinoa and aqueous extract of black quinoa as active ingredients; The product comprises 3 - 24 parts of alcohol extract of black hulless barley, 2 - 16 parts of aqueous extract of black hulless barley, 3 -10 parts of alcohol extract of black quinoa and 5 - 7 parts of aqueous extract of black quinoa.

According to a preferred embodiment of the disclosure, the composition comprises 12 parts of alcohol extract of black hulless barley, 8 parts of aqueous extract of black hulless barley, 4 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa.

The superoxide dismutase agonist is a product that can only increase the content of superoxide dismutase (SOD) in organisms.

Peroxidation reaction occurs when radicals in vivo act on lipid, and produces the end product - malondialdehyde. Malondialdehyde can cause cross-linked polymerization of biomacromolecules such as protein and nucleic acid, and has cytotoxicity. The composition according to the disclosure can reduce the content of malondialdehyde (MDA) in mouse serum, which shows that the composition according to the disclosure can reduce the occurrence of lipid peroxidation reaction and has antioxidation.

The disclosure has the following beneficial effects: (1) The extraction method of the disclosure is simple, easy and time-saving. It can effectively reduce the cost of products related to black hulless barley and black quinoa, and is worth popularizing.

(2) The disclosure provides a series of compositions comprising the aqueous extract of black hulless barley, alcohol extract of black hulless barley, aqueous extract of black quinoa and alcohol extract of black quinoa, and proves through experiments that the compositions can increase the SOD content and reduce the MDA content, have antioxidant activity, and can be used for preparing anti-oxidation products.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS HU500683 The following is a clear and complete description of the technical solutions in the disclosure. Obviously, the embodiments are only some of rather than all of the embodiments of the disclosure. Other embodiments based on the disclosure as acquired 5 by general technicians in the field without making creative efforts shall fall within the protection scope of the disclosure. Embodiment 1 Preparation of black hulless barley extract and black quinoa extract (1) Preparation of alcohol extract of black hulless barley: Add black hulless barley powder dried to constant weight into 95% v/v, 85% v/v, 75% v/v, 65% v/v, 55% v/v, 45% v/v and 35% v/v ethanol-water solution successively to conduct reflux extraction 7 times; filter the solution and keep the dregs; mix the extract and recover and dry the ethanol to obtain the alcohol extract of black hulless barley. After measurement, the yield rate is about 29.93%.

(2) Preparation of aqueous extract of black hulless barley: Take the dregs in step (1) to be cooked with water 5 times and filter the solution; mix, concentrate and dry the filtrate to obtain the aqueous extract of black hulless barley. After measurement, the yield rate is about 20.18%.

(3) Preparation of alcohol extract of black quinoa: Add black quinoa powder dried to constant weight into 95% v/v, 85% v/v, 75% v/v, 65% v/v, 55% v/v, 45% v/v and 35% v/v ethanol-water solution successively to conduct reflux extraction 7 times; filter the solution and keep the dregs; mix the extract and recover and dry the ethanol to obtain the alcohol extract of black quinoa. After measurement, the yield rate is about

21.38%.

(4) Preparation of aqueous extract of black quinoa: Take the dregs in step (1) to be cooked with water 5 times and filter the solution; mix, concentrate and dry the filtrate to obtain the aqueous extract of black quinoa. After measurement, the yield rate is about

15.37%.

The yield rate can fluctuate normally within 5%.

Embodiment 2

Take 12 parts of alcohol extract of black hulless barley, 8 parts of aqueous extract 500685 of black hulless barley, 4 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa prepared according to Embodiment 1 and mix them evenly to obtain a composition of the disclosure.

Embodiment 3 Mix 12 parts of alcohol extract of black hulless barley, 8 parts of aqueous extract of black hulless barley, 4 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa prepared according to Embodiment 1 evenly with a moderate quantity of microcrystalline cellulose, and dry and fill them into capsules to obtain capsula of the composition of the disclosure.

Embodiment 4 Detection of oxidase content Male ICR mice weighing 20-22 g are randomly divided into normal control group, aging model group, vitamin E group (positive control group) and drug groups with different compatibility, with 10 mice for each group, so as to verify the antioxidation of the extract of the disclosure.

A subacute peroxidation injury model is made by injecting subcutaneously normal saline of the same volume into mice in the normal control group every day and 150 mg (/kg-d) D-galactose into mice in other groups for 55 days.

A behavioral test shows that the modeling is successful. 150g (/kg-d) D-galactose is administered intragastrically on the next day after the first injection.

The same dose of the composition comprising black hulless barley extract and black quinoa extract with different ratios is administered to the mice in the experimental groups. 50 mg (/kg-d) vitamin E is administered to the mice in the positive control group, and drinking water is administered to those in the normal control group and aging model group.

Mice in each group are raised in the same room and different cages, and can eat freely.

After the last administration, the mice go through a fast for 24 hours.

The eyeballs are then taken for blood sampling, and the mice are killed by cutting off their heads.

The plasma is centrifuged at 3500 r/min at 4°C for 10 min to separate the serum.

The levels of SOD and MDA are determined according to the instructions in the kit.

Table 1 Effect of Black Hulless Barley Extract and Black Quinoa Extract on SOD and MDA in Serum of Mice

Group Matter administered SOD/U-mL-1 MDA/ nmol -mL-1 200683 Normal control Drinking water 166.18+15.16 6.25+0.57 group Aging model Drinking water 11.11+0.95 group 130.73+12.87 Vitamin F Vitamin F 208.71+18.21 7.10+0.92 group Group 1 Alcohol extract of black hulless barley 170.22+17.05 6.07+1.20 Group 2 Aqueous extract of black hulless barley 129.40+11.92 9.18+1.20 Group 3 Alcohol extract of black quinoa 163.91+17.10 6.55+0.78 Group 4 Aqueous extract of black quinoa 126.049+11.97 10.30+0.95 Group 5 Alcohol extract of black hulless barley : aqueous extract of black hulless barley = 3:2 162.59+16.20 6.65+1.11 Group 6 Alcohol extract of black hulless barley : aqueous extract of black hulless barley = 6:2 158.27+14.40 6.98+0.86 Group 7 Alcohol extract of black hulless barley : aqueous extract of black hulless barley = 12:2 150.97+13.97 7.53+0.64 Group 8 Alcohol extract of black hulless barley : aqueous extract of black hulless barley = 7:5 130.73+11.52 9.07+1.07 Group 9 Alcohol extract of black hulless barley : aqueous extract of black hulless barley = 3:8 121.49+10.89 10.54+1.15 Group 10 Alcohol extract of black quinoa : aqueous extract of black quinoa = 7:5 153.63+15.11 7.33+1.21 Group 11 Alcohol extract of black quinoa : aqueous extract of black quinoa = 14:5 138.69+12.06 8.47+0.95 Group 12 Alcohol extract of black quinoa : aqueous extract of black quinoa = 28:5 126.79+10.99 10.14+1.28 Group 13 Alcohol extract of black quinoa : aqueous extract of black quinoa = 7:10 110.67+11.02 10.64+0.86

-— TTT tubes

Group 14 Alcohol extract of black quinoa : aqueous extract of black quinoa = 7:20 110.21+9.38 10.55+1.16 Group 15 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 3:2:7:5 (equivalent to black hulless barley : black quinoa = 1: 3.3) 188.14+16.18 4.71+0.88 Group 16 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 3:2:14:10 (equivalent to black hulless barley : black quinoa = 1:6.5) 177.85+15.16 5.49+1.02 Group 17 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 6:4:7:5 (equivalent to black hulless barley : black quinoa = 1:1.7) 208.71+18.99 3.14+0.62 Group 18 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 12:8:7:5 (equivalent to black hulless barley : black quinoa = 1.3:1) 195.44+20.03 4.15+0.77 Group 19 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 24:16:7:5 (equivalent to black hulless barley : black quinoa = 2.5:1) 194.77+18.17 4.20+0.56 Group 20 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract 196.76£16.89 4 05+0.57 of black quinoa : aqueous extract of black 200683 quinoa = 3:2:4:5 (equivalent to black hulless barley : black quinoa = 3.3:1) Group 21 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 12:8:4:5 (equivalent to black hulless barley : black quinoa = 1.2:1) 227.62+20.52 2.70+0.38 Group 22 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 12:8:10:5 (equivalent to black hulless barley : black quinoa = 1.2:1) 186.48+17.65 4 83+0.80 Group 23 Alcohol extract of black hulless barley : aqueous extract of black hulless barley : alcohol extract of black quinoa : aqueous extract of black quinoa = 6:6:7:5 (equivalent to black hulless barley : black quinoa = 1.1:1) 178.51+16.40 5.44+0.8.9 © According to the data in Table 1, when aqueous extract of black hulless barley and ~~ aqueous extract of black quinoa are used alone, the content of malondialdehyde (MDA) decreases, but the content of superoxide dismutase (SOD) decreases too.

When alcohol extract of black hulless barley and alcohol extract of black quinoa are used alone, the SOD content increases and the MDA content decreases, but the effect is not obvious.

When alcohol extract of black hulless barley, aqueous extract of black hulless barley, alcohol extract of black quinoa and aqueous extract of black quinoa are used together at a certain ratio, the SOD content in mouse serum is higher than the content that can be acquired by using any single extract or two kinds of extract, and the MDA content is lower than the content that can be acquired by using any single extract or two kinds of extract.

The results show that a composition comprising alcohol extract of black hulless barley, aqueous extract of black hulless barley, alcohol extract of black quinoa and aqueous extract of black quinoa can work together to improve the antioxidant activity of the extract.

When the ratio of alcohol extract of black hulless barley, aqueous extract LU500683 of black hulless barley, alcohol extract of black quinoa and aqueous extract of black quinoa is 12: 8: 4: 5, the composition has the strongest antioxidation.

The above descriptions are merely embodiments of the disclosure and not used for limiting the protection scope of the disclosure, and any equivalent process or equivalent structure transformation, made by using contents of the description of the disclosure or applied to other relevant technical fields directly or indirectly, shall be regarded as falling into the protection scope of the disclosure.

Claims (10)

CLAIMS LU500683

1. A purpose of a composition in the preparation of anti-oxidation products, wherein the composition comprises 3 - 24 parts of alcohol extract of black hulless barley, 2 - 16 parts of aqueous extract of black hulless barley, 3 -10 parts of alcohol extract of black quinoa and 5 - 7 parts of aqueous extract of black quinoa.

2. The purpose according to Claim 1, wherein the mass ratio of the alcohol extract and aqueous extract of black hulless barley is 1.5-2.4:1 (preferably 1.5:1), and the mass ratio of the alcohol extract and aqueous extract of black quinoa is 0.8-2:1 (preferably

0.8:1).

3. The purpose according to Claim 1, wherein the composition comprises 12-24 parts of alcohol extract of black hulless barley, 8-16 parts of aqueous extract of black hulless barley, 4-7 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa.

4. The purpose according to Claim 3, wherein the composition comprises 12 parts of alcohol extract of black hulless barley, 8 parts of aqueous extract of black hulless barley, 4 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa.

5. The purpose of any one of claims 1-4, wherein the alcohol extract may be one or a combination of methanol, ethanol, butanol or pentanol.

6. The purpose according to Claim 5, wherein the alcohol extract is ethanol, specifically, 20~100% v/v ethanol-water solution, and 35~95% v/v ethanol-water solution is preferred.

7. The purpose of any one of claims 1-6, wherein the product is a superoxide dismutase agonist and/or a product for reducing malondialdehyde content in vivo.

8. The purpose according to Claim 7, wherein the product is a product for scavenging oxygen radicals in vivo.

9. An anti-oxidation product, wherein the product is prepared by using alcohol extract of black hulless barley, aqueous extract of black hulless barley, alcohol extract of black quinoa and aqueous extract of black quinoa as active ingredients; the product comprises 3 - 24 parts of alcohol extract of black hulless barley, 2 - 16 parts of aqueous extract of black hulless barley, 3 -10 parts of alcohol extract of black 7500689 quinoa and 5 - 7 parts of aqueous extract of black quinoa.

10. The product according to Claim 9, comprising 12 parts of alcohol extract of black hulless barley, 8 parts of aqueous extract of black hulless barley, 4 parts of alcohol extract of black quinoa and 5 parts of aqueous extract of black quinoa.