KR20030033723A - Extracts derived from plant that have anti-oxidation activity - Google Patents

Abstract

PURPOSE: Provided is an extract extracted from plants having anti-oxidant activity, which can be used as an agent for preventing cancer and aging, an additive of health supporting food, or an additive of a drink. CONSTITUTION: The extract is extracted from at least one plant selected from the group consisting of Eugenia caryophyllata, Nauclea officinalis, Capsella bursa-pastoris, Terminalia chebula, Sanguisorba officinalis, Chloranthus glaber, Cassiae semen, Celtis jessoensis, Ledebouriella seseloides, Punica granatum, Carpinus coreana, Symplocos prunifolia, Carpinus laxiflora, Cornus walteri, Platycarya strobilacea, Juniperus oxycedrus, Pimento racemosa, Capsicum annuum var. angulosum, and Eugenia caryophyllata by using a solvent such as a low alcohol, an organic solvent, or distilled water.

Description

Plant-derived extract with antioxidant activity {EXTRACTS DERIVED FROM PLANT THAT HAVE ANTI-OXIDATION ACTIVITY}

[TECHNICAL FIELD OF THE INVENTION]

The present invention relates to a plant-derived extract having an antioxidant activity, and more particularly to a plant-derived extract having an antioxidant activity that can control the active oxygen causing the aging and adult disease in humans.

[Private Technology]

One of the major changes that occur during food processing and distribution cooking is oxidation. Oxidation of lipids leads to other changes affecting food quality, benefit, safety, color and flavor. When a small amount of carbonyl compounds are produced by such oxidation, they may give a characteristic flavor in cooked foods, but they usually cause food deterioration. According to thermodynamic theory, oxygen and unsaturated fatty acids cannot react, but oxidation is directly and indirectly promoted through free radical mechanisms such as light, heat, enzymes, photosensitizers and transition metals. 1996. Development and Use of New Natural Antioxidants Food Technology 9 (1))

Oxygen has a stable molecular state of ground state triplet oxygen due to various physical, chemical and environmental factors such as enzymes, reduction metabolism, chemicals, pollutants, and photochemical reactions. Oxygen toxicity fatal to the body when converted to highly reactive active oxygen such as hydroxyl radicals (HO ·), hydrogen peroxide (H ₂ O ₂ ), and singlet oxygen Causes These free radicals are non-selective and irreversible destructive action on cell components such as lipids, proteins, sugars, DNA, etc., and directly or indirectly by being involved in physiological changes such as aging and a wide range of biological phenomena such as cancer, arthritis, and diabetes. (Halliwell, B. 1991. Drug antioidant effects.Drugs. 42: 596-605)

The study of antioxidants began in earnest in 1969 when McCord and Fridovic discovered the superoxide dismutase (SOD), an enzyme that eliminates superoxide radicals. Since then, research into antioxidants has been shifting from research for developing antioxidants as food additives to finding antioxidants as anti-aging and disease treatment agents.

Antioxidants that have been developed and used so far include synthetic antioxidants such as tert-butylhydroxytoluene (BHT) and tert-butylhydroxyanisol (BHA), natural antioxidants such as tocopherol, ascorbic acid, carotenoids, flavonoids, tannins, and SOD, catalase, glutathione And antioxidant enzymes. However, these antioxidants are limited to use due to various problems such as toxicity, low activity and limit of use. Therefore, research is being actively conducted to search for safer and stronger antioxidants from natural or microbial metabolites. Free radical scavengers or free radicals and activities that directly eliminate free radicals that cause direct oxidative damage in vivo in lipid peroxidation inhibitors It has been extended to more active antioxidants such as prophylactic antioxidants that inhibit the oxygen production reaction itself, and the focus is on practical, safe and low molecular weight natural antioxidants.

However, until now, natural and synthetic antioxidants have been developed and used as medicines or research reagents, but there are limitations in their use as medicines for the treatment of aging due to oxidative stress and various diseases due to weak activity, toxicity and problems in use. Therefore, the development of a new natural antioxidant that can solve the problems of the existing antioxidants is required.

In order to solve the problems of the prior art, it is an object of the present invention to provide a natural plant extract showing a strong antioxidant activity.

It is another object of the present invention to provide a plant-derived antioxidant which is not toxic.

In order to achieve the above object, the present invention is a clove, mountainous, wasabi, let's go, cucumber grass, bamboo shoots, the deficiency, larvae, windproof, pomegranate, hawthorn, black ash, roe, dogwood, copper, card, Provided is an antioxidant composition comprising an extract extracted from a plant selected from the group consisting of bay, pimentoberry and clove.

The present invention also provides a food comprising the antioxidant composition.

Hereinafter, the present invention will be described in detail.

Antioxidants of the present invention are Eugenia carriophillata , Paeonia suffruticosa , Nauclea officinalis , Capsella brusa-pastoris , Terminalia chebula , Cucumber ( Sanguisorba officinalis ), Chloranthus glaber , Cassiae semen , Celtis jessoensis , Windproof ( Ledebouriella seseloides ), Pomegranate ( Punica granatum ), Pine needles ( Carpinus coreana ), Black ash ( Symplocos priunifolia ), Horsetail ( Capinus laxiflora ) Tree ( Cornus walteri ), Copper ( Playcarya strobilacea ), Juniperus oxycedrus , Pimento racemosa , Capsicum annum burr. Angurosum ( Capsicum annuum var. Angulosum ) and Eugenia caryophyllata ( Eugenia caryophyllata ) is an extract extracted from at least one plant selected from the group consisting of.

The extract is extracted by a conventional extraction method, preferably extracted with a low alcohol, organic solvent, or distilled water. Low cost alcohols are alcohols having 1 to 5 carbon atoms, such as methanol, butanol, propanol, isopropanol and the like, and organic solvents include hexane, ethyl acetate, chloroform and the like. More preferably, it is a crude methanol extract or an oil obtained by extracting methanol as a solvent.

The plant to be extracted may use the whole plant, or may use flowers, buds, fruits, seeds, roots, etc., which are frequently used for medicine or drinking. Cloves, Eugenia caryopilata, buds are preferred, bark skins, root peels are preferred, mountain roots are preferably roots, and saplings are plants, Gaza and Capsicum annuum. Angurosum is preferably a fruit, cucumber grass, bamboo shoots, a deficiency, a fern, and a windproof seed are preferred, pomegranate, hawthorn, black ash, acer, dogwood, juniperus oxidus, eugenia kari Yopilata and pimento racemosa are preferably leaves. However, the object to be extracted is not limited to the above sites. Juniper Oxidus, Pimento Racemosa, Capsicum Annum Burr. Angurosum or Eugenia caryophyllatacard can be used as commercially available Cade, Bay, Pimentoberry and Clove.

Methanol crude extracts and essential oils of the present invention were confirmed to have antioxidant activity by DPPH radical scavenging method, and similar activity to synthetic antioxidants.

The crude methanol extract and the essential oil of the present invention have anticancer activity and can be used for various purposes. The drug is preferably used as an anticancer agent or an antioxidant, and is preferably used for the purpose of preventing disease and promoting health by using it as a food supplement, beverage, or food additive. It can also be used as a prophylactic or therapeutic agent for diseases caused by the oxidative power of free radicals, in particular adult diseases and aging. Plants that are the raw material of the crude methanol extract are used for medicinal or edible foods, and thus the crude methanol extract is not harmful to the human body.

In addition, the composition containing the crude methanol extract of the present invention may be used alone or methanol extract of plants, it may further comprise a pharmaceutically acceptable carrier. In this case, the content of the plant extract is preferably adjusted according to the use or method, it is preferable to include 0.01 to 20% by weight. The formulation is preferably a powder, a medicament, an injection, a tablet, a suspension, etc., and the dosage is not limited to a certain amount, but when used as a medicament 100 ug to 200 ug per day This is preferred.

Hereinafter, examples of the present invention will be described. The following examples are only for illustrating the present invention and the present invention is not limited to the following examples.

Example 1: Preparation of Plant Extract

A crude crude extract of methanol was prepared by varying the portion extracted according to each plant. Cloves are buds, Bark skins are rooted, Sans are roots, Sanctions are plants, Fruits are barley, Cucumbers, bamboo shoots, clarifiers, pinnacles, and windproof seeds, pomegranates, pine trees, Black ash, horn and dogwood prepared leaves as samples.

Methanol crude extract production method was carried out in the same manner for each plant.

Plants were obtained and air dried in a room, and each was ground with a mill to make a powder. 50 g of powder samples were placed in a 500 ml Erlenmeyer flask, and 300 ml of methanol per 100 g of plants was poured and left at room temperature and dark. After 3 days, the resultant was filtered under reduced pressure and concentrated under reduced pressure at 40 ° C. using a vacuum rotary concentrator to obtain a crude methanol extract.

Example 2: Plant Essential Oil Preparation

Antioxidant activity was confirmed by purchasing essential oils sold without extracting directly from plants. Essential oils are cade oil, bay, cloveleaf, clovebud, pimentoberry and purchased from gin perfume.

Example 3: Antioxidant Activity Assay

DPPH lycal scavenging was performed using the crude plant methanol extract of Example 1 and the natural essential oil of Example 2, and the antioxidant activity of each sample was measured.

After taking 10 mg of the sample and applying 10 ml of methanol, each was diluted with 100 µl / ml methanol, 50 µl / ml methanol, 25 µl / ml methanol, and 12.5 µl / ml methanol. 0.5 ml of methanol containing 1.5 × 10 ^-4 M / ml of DPPH (Sigma, St. Louis, Mo, USA) solution was mixed in 2 ml of diluent, and allowed to stand at room temperature for 30 minutes, followed by absorbance at 520 nm. ) Was measured. As a control, BHA ( tert- butyl-4-hydroxyanisole), a synthetic antioxidant, and vitamin C (L-ascorbic acid), a natural antioxidant, were used. Antioxidant activity was presented as a concentration (EC ₅₀ ) of the sample showing a 50% decrease in absorbance, and each sample was repeated three times to calculate an average. Table 1 below shows the antioxidant activity of natural essential oils and crude plant methanol extracts by DPPH radical scavenging method.

Plant name EC ₅₀ (μg per ml methanol) cloves Eugenia carryophillata Flower buds <12.5 Osuyu Evodia rutaecarpa Fruit <12.5 Mountain Nauclea officinalis Root <10 Vegetables (cereal) Capsella brusa-pastoris Plant <10 Go Terminalia chebula Fruit <10 Cucumber Sanguisorba officinalis strain <10 Bamboo shoots Chloranthus glaber strain <10 A defector Cassiae semen strain <10 Weathered tree Celtis jessoensis strain <12.5 Windproof Ledebouriella seseloides strain <12.5 Pomegranate Punica granatum Lobe 4.6 Hawthorn Carpinus coreana Lobe 8.4 Black ash tree Symplocos priunifolia Lobe 6.0 West tree Capinus laxiflora Lobe 5.8 Dogwood Cornus walteri Lobe 4.9 Khed 18.8 Bay 7.6 Cloblip 4.9 Clovebird 5.1 Pimento Berry 6.0 Vitamin c 2.3 BHA 5.8

In Table 1, antioxidants high in ked (18.8 ug / ml), bay (7.6 ug / ml), clovelip (4.9 ug / ml), clovebird (5.1 ug / ml), pimentoberry (6.0 ug / ml) Activity was shown. Plant methanol crude extract showed high antioxidant activity of less than 12.5 ug / ml of clove buds, less than 10 ug / ml of bark extracts of the bark root, and less than 12.5 ul / ml of sorghum fruit. In addition, methanol crude extracts of the roots of the roots, chaehwa, Gaja, Cucumber, Cucumber, and Cultivated seeds showed high antioxidant activity of less than 10 ug / ml, respectively. , 4.6 ug / ml of pomegranate leaf, 8.4 ug / ml of pine needles, 6.0 ug / ml of black ash leaf, 5.8 ug / ml of horn leaf, and 4.9 ug / ml of horsewood leaf.

As mentioned above, the cloves of the present invention, bark bark, tree, horseradish, let's go, cucumber grass, bamboo shoots, the deflector, windmill, windproof, pomegranate, pine needles, black ash, rhododendron, dogwood, copper wood , Extracts from plants selected from the group consisting of Juniper Oxidus, Pimento Racemosa, Capsicum annum burr, Angulosum and Eugenia carrillopila have excellent antioxidant activity. Therefore, the crude methanol extract of the present invention may be used as a prophylactic agent for cancer and aging, as an additive material for health supplement foods, or as an additive material for beverages.

Claims (6)

Cloves, trees, horseradish, let's go, cucumber grass, bamboo shoots, gnarlers, pinwheel, windproof, pomegranate, pine needles, black ash, rhorus, dogwood, copper, Juniperus oxycedrus , blood Mentor Pimento racemosa , Capsicum Annum Burr. An antioxidant composition comprising an extract extracted from a plant selected from the group consisting of Angurosum ( Capsicum annuum var. Angulosum ) and Eugenia caryophyllata . The antioxidant composition according to claim 1, wherein the extract is extracted using a low alcohol, an organic solvent or distilled water as a solvent. The method of claim 1, wherein the extract is a group consisting of cloves, seedlings, horseradish, let's go, cucumber grass, bamboo shoots, bladder, larvae, windproof, pomegranate, bracken, black ash, roe, dogwood and copper Antioxidant composition which is a crude methanol extract extracted with methanol at least one plant selected from. The method of claim 1, wherein the extract is Juniperus oxycedrus , Pimento racemosa , Capsicum annum bur. An antioxidant composition is an oil extracted from a plant selected from the group consisting of Angurosum ( Capsicum annuum var. Angulosum ) and Eugenia caryophyllata (Eugenia caryophyllata ). Food comprising the antioxidant composition of claim 1. 6. The food of claim 5, wherein the food is a food additive, beverage or dietary supplement.