KR20200120465A - Composition for preventing or treating liver damage by alcohol which is excellent in hangover resolution - Google Patents

Abstract

The present invention relates to a composition for preventing or treating liver damage by alcohol which is excellent in hangover resolution by containing hydrogen absorbing metal capable of generating hydrogen in the body by absorbing hydrogen to metal, a green onion sprout hot water extract freeze-dried powder, vitamin C, and a hot water extract freeze-dried powder with the same weight ratio mixture of Hibiscus smanihot and Asimina triloba leaves. The present invention has an effect of inhibiting liver damage, and lowers a hepatitis index, thereby being able to be used as the composition for preventing or treating various liver diseases. In addition, the present invention is very useful as the composition for protecting liver or resolving hang over taken before/after drinking by relieving headache, vomiting symptoms, etc., the next day after drinking.

Description

Composition for preventing or treating liver damage by alcohol which is excellent in hangover resolution}

The present invention relates to a composition for preventing or treating liver damage due to alcohol, which is excellent in relieving hangovers, and more particularly, hydrogen storage capable of generating hydrogen in the body by storing hydrogen in a metal other than gaseous hydrogen or hydrogen dissolved liquid. It is excellent in relieving hangovers containing metal and vitamin C, and relates to a composition for preventing or treating liver damage caused by alcohol.

The liver is a central organ that metabolizes sugars, proteins, and fats, and is an important organ responsible for excreting substances that have entered the body through oxidation, reduction, hydrolysis and conjugation reactions. However, the liver is known as a “silent organ” since liver disease is found after a significant deterioration without pain or subjective symptoms in the early stages.

When liver disease worsens, constipation, irritable bowel syndrome, or color shift in stool may appear, and changes such as persistent vomiting may occur in patients with liver damage, and sudden weight loss may occur.

In addition, due to liver dysfunction, body fluids that have inhibited the body's ability to produce proteins and circulate may descend to the lower body by gravity and cause circulatory problems such as swelling of the legs, ankles and feet, and liver failure, such as fatigue, chronic fatigue, and memory loss. The most common symptoms of injury can occur.

Signs of severe liver damage include swelling of the abdomen due to accumulation of fluid (ascites) in the abdomen as the levels of albumin and protein in the blood and body remain high, and jaundice in which the skin and eyes become yellow is also liver damage. Is a sign of.

In addition, there may be pain in the upper right abdomen or in the lower right part of the ribcage (liver pain), urine may turn dark yellow due to increased bilirubin levels in the bloodstream, and itching or peeling (peeling) of the skin may occur. .

Most of the causes of liver damage are hepatitis virus (A, B, C, etc.), alcohol (drinking), and non-alcoholic fatty liver, and according to statistics, the cost of treatment caused by alcoholic liver disease in Korea is increasing every year. In addition, even when analyzing the statistics of a domestic life insurance company, among the causes of death, the number of deaths from alcoholic liver disease has increased by 7 times compared to 10 years ago. Alcoholic liver disease caused by continuous alcohol intake is emerging as a social problem around the world, including in Korea. In particular, if alcoholic fatty liver and hepatitis persist, recovery from liver fibrosis and cirrhosis can not be expected, so liver transplantation is the only one. It remains a treatment method.

Meanwhile, according to statistics from the National Cancer Center, 60-70% of Korean hepatitis virus carriers are hepatitis B carriers, and 75% of liver cancer patients are hepatitis B carriers, and 5-10% of the total population is chronic hepatitis B. There are statistics that it is a virus carrier, and hepatitis B C virus and various chronic diseases of the liver are the causes of liver cancer, the most frequent malignant tumor in the world.

Conventional liver disease treatments currently include liver function adjuvants, antiviral agents, hepatocyte promoters, immunosuppressants, fibrosis inhibitors, biphenyldimethyldicarboxylate, interferon, etc., but these drugs have low therapeutic effect, high side effects, and supplements. It is known that the combined administration not only increases the therapeutic effect, but also shows side effects. Therefore, research on the development of functional ingredients for improving liver function with less side effects is needed.

The drinking rate of adult men over 20 years old in Korea is very high at 83.3%, and the drinking rate of women is also rapidly increasing to 54.9%, and they are seriously exposed to excessive drinking due to the wrong drinking culture. The damage caused by excessive consumption of alcohol not only causes liver disease and other diseases, but also negatively affects the emotions and lives of individuals, families, and society, causing enormous economic and social losses in the country.

Excessive drinking causes many types of side effects, such as thirst, general malaise, fatigue, memory loss, bloating, indigestion, vomiting, diarrhea, and vitamin deficiency, and increases the risk of alcoholism. do.

Among liver diseases, diseases caused by chronic drinking are largely divided into alcoholic fatty liver, alcoholic hepatitis, and alcoholic cirrhosis, but only one disease is rarely seen in one person, and each disease appears to varying degrees for each individual.

It is the most common cause of cirrhosis in the United States and is the second most common cause of cirrhosis after viral hepatitis in Korea.

When alcohol is ingested, normal alcohol metabolism is absorbed in the stomach or small intestine, and 10% of it is excreted through breathing, sweat, and urine, and the remaining 90% of alcohol enters blood vessels and is metabolized in the liver (M Nakanishi; Saishin Igaku, 31, p2086, 1976) Subsequently, alcohol dehydrogenase (ADH) present in hepatocytes oxidizes alcohol to acetaldehyde, and acetaldehyde is decomposed into acetic acid by acetaldehyde dehydrogenase in hepatocytes. It is transferred to the entire body's muscle or fat tissue and finally decomposed into carbon dioxide and water.

However, if this decomposition metabolic process is not smooth due to excessive drinking, acetaldehyde accumulates and cerebrovascular contraction occurs, resulting in a heavy head and hangover such as headache and heartburn.

Individually recognized functional ingredients that help liver health that help solve such problems include milk thistle extract, broccoliprout powder, shiitake mushroom mycelium extract, bokbunja extract powder, bellflower extract, etc., and protect the liver from alcohol damage. Functional ingredients that are helpful for health include extracts from dulcis dulcis and fermented kelp extracts (Ministry of Food and Drug Safety, 2018).

As a prior art for a composition for liver protection, Korean Patent Registration No. 10-0477957 ([New pyrrole derivative having liver protection activity isolated from Gugija and a composition containing the same), Korean Patent No. 10-0633851 (heat drying treatment) Liver protection or liver disease prevention and treatment composition containing the wild garlic extract as an active ingredient), Republic of Korea Patent No. 10-1106499 (food composition for liver protection effect including extract of sprig Liver toxicity recovery effect (Jeong-su et al., Journal of Korean Society of Sericulture; 50(2), p93, 2012), Liver protection and hepatocyte regeneration effect of beetle (Eunji Lee et al., Journal of Life Science; 25(3), p307, 2015), cricket Of the liver (Miyoung Ahn et al., Journal of Food Science and Technology; 34(4), p684, 2002).

Regarding hangover relieving, Korean Patent Application Publication No. 2002-0081995 (Hangover Relief and Liver Function Improvement Agent Using Blended Foods and Its Manufacturing Method), Korean Patent Application Publication No. 2002-0064151 (Hepatotoxicity and hangover relieving activity isolated from Hangover trees) In patents such as lower alcohol-insoluble extract fractions and polysaccharide substances and compositions containing the same), preparations derived from various plants such as rice snow extract (gurume), alder tree, oak herb juice, brown flower, and milk thistle extract are effective for hangovers. Although reported to have been reported, they are only ineffective or insignificant in some of the various changes caused by drinking.

Republic of Korea Patent Registration No. 10-0477957 (Name of invention: novel pyrrole derivative having liver protective activity isolated from wolfberry and composition containing it, Applicant: Seoul National University Industry-Academic Cooperation Foundation, Registration Date: March 10, 2005) Republic of Korea Patent Registration No. 10-0633851 (Name of invention: composition for liver protection or prevention and treatment of liver disease containing heat-dried acid garlic extract as an active ingredient, Applicant: Sangji Academy, registered date: 2006 10 April 04) Republic of Korea Patent Registration No. 10-1106499 (Name of the invention: food composition for liver protection effect containing extract of sprig of dulcis sprig, Applicant: Heung-gun Jang, registration date: January 10, 2012) Korean Patent Registration No. 10-1852840 (Name of the invention: composition for liver protection containing slugs enzyme treatment, Applicant: Hanmi Corporation, registration date: April 23, 2018) Korean Patent Registration No. 10-0453575 (Name of the invention: Food composition for liver protection, tonic fatigue recovery, promotion of alcohol metabolism, suppression of the production of lipid peroxide containing cricket itself or its extract, Applicant: Republic of Korea, registration date: 2004 11th of month) Republic of Korea Patent Publication No. 10-2002-0081995 (Name of the invention: hangover relief and liver function improvement agent by blended food and its manufacturing method, Applicant: Drigo Co., Ltd., Publication date: October 30, 2002) Republic of Korea Patent Laid-Open Patent No. 10-2002-0064151 (Name of the invention: lower alcohol-insoluble extract fraction and polysaccharide substance having liver toxicity and hangover relieving activity isolated from dulcis tree, and composition containing the same, Applicant: Life Insurance Co., Ltd. Tree, release date: August 7, 2002)

Therefore, the problem to be solved by the present invention is to provide a composition for preventing or treating liver damage caused by alcohol and is excellent in relieving hangovers.

In order to solve the above problems, the present invention

Green onion sprout hot water extract freeze-dried powder;

Freeze-dried powder extracted with hot water extracted with the same weight ratio of Geumhwagyu and Popo tree leaves: and

A health functional food composition for preventing or improving liver protection, hangover relief, or alcoholic liver disease, characterized in that it contains a hydrogen occluding metal as an active ingredient.

In the health functional food composition according to the present invention, the hydrogen occluding metal is preferably magnesium hydride.

Health functional food composition according to the present invention when the hydrogen occlusion metal 100 in weight ratio, 150 to 200 green onion sprout hot water extraction freeze-dried powder 150 to 200 hot water extraction freeze-dried powder mixture with the same weight ratio of Geumhwagyu and Popo tree leaves It features.

The health functional food composition according to the present invention further comprises vitamin C, and when the hydrogen occlusion metal is 100 in a weight ratio, hot water extraction of green onion sprouts, freeze-dried powder 150 to 200, hot water extraction of a mixture in the same weight ratio of Geumhwagyu and P. It is characterized in that the freeze-dried powder 150 to 200, vitamin C 10 to 20.

In the health functional food composition according to the present invention, the alcoholic liver disease means alcoholic hepatitis, alcoholic fatty liver or alcoholic cirrhosis.

The composition of the present invention as described above has the effect of inhibiting liver damage, can be used as a composition that can prevent or treat various liver diseases by lowering the hepatitis index, and has excellent alcohol decomposition effect and acetaldehyde decomposition effect, and after drinking It is also very useful as a composition for protecting the liver or relieving hangovers taken before/after drinking by relieving headaches and vomiting symptoms the next day.

Hereinafter, the present invention will be described in detail.

The present invention is a hydrogen storage metal as an active ingredient; Vitamin C; And it provides a composition for liver protection or hangover, characterized in that it comprises a mixture of hot water extracted freeze-dried powder in the same weight ratio of Geumhwagyu and Popo tree leaves as an active ingredient.

The present invention protects the liver or relieves hangovers in a combination with hydrogen, vitamin C, and the same weight ratio mixture of Geumhwagyu and Popo tree leaves and freeze-dried powder extracted with hot water, furthermore, alcoholic liver disease represented by alcoholic hepatitis, alcoholic fatty liver or alcoholic cirrhosis It was completed by confirming the effect of preventing or treating hydrogen. In particular, as a method of absorbing hydrogen into the body, the present invention uses a method of inhaling hydrogen in gaseous form or drinking hydrogen water dissolved in hydrogen. The intake of hydrogen in a solid form, rather than a method, generates hydrogen in the body organs such as the stomach and intestines, allowing the hydrogen to be absorbed in the body.

In the case of such a method, it is possible to achieve hydrogen absorption in the body at a higher concentration than the conventional method and to maintain a constant concentration of supplied hydrogen, thereby doubling the internal effect of hydrogen and increasing the convenience of production and storage. It has the advantage of high compliance of the ingested patient, reducing the cost of generating hydrogen, and in particular, it has the advantage of easy to make powder-type complex formulations such as powders, tablets, granules, capsules, and pills.

The hot water extraction freeze-dried powder of a mixture of the same weight ratio of Geumhwagyu and Popo tree leaves used in the present invention is prepared as follows.

After mixing the whole Geumhwagyu including the roots and the leaves of Popo tree at the same weight ratio, the hot-water extract was freeze-dried to prepare a powder containing no moisture after hot-water extraction, and the hot-water extraction method and freeze-drying are the present invention. As long as it is widely known in the technical field to which it belongs, it can be used without particular limitation.

In the present invention, the Geumhwagyu is an annual herbaceous plant belonging to the genus Hibiscus. The Geumhwagyu is also called a wild bouillon and is a medicinal plant carried on the primrose, and has been restored and cultivated in China. Seeds have been introduced and cultivation is taking place in Korea. Geumhwagyu grows beyond the height of a person in July-August. The flower blossoms are usually 16-18cm in diameter, the petals are yellow, thin, and scarlet. The leaves are dark blue, and the leaves are very beautiful. Geumhwagyu is said to be harvested in a timely manner because the flowering time is only about six hours. Geumhwagyu flowers are picked from the end of July, shredded, dried, and powdered. Raw materials for cosmetics and tea are used, and the leaves of Geumhwagyu also contain various physiologically active substances and are used for various purposes.

The popo tree used in the present invention is a deciduous broad-leaved tree native to North America. The flowering time is around April and the fruit is harvested around September to October. This fruit has a refreshing taste like a combination of banana, pineapple, and mango, and has good sugar content and protein. It has a high content and is recently known to have a strong anti-cancer component, and is attracting attention as a medical and health food.

In addition, vitamin C used in the present invention can be used without particular limitation as long as it is widely known in the technical field to which the present invention belongs, and the hydrogen occluding metal is as follows.

Metals generally have hydrogen on their surface or inside. Among them, palladium and magnesium can hold a large amount of hydrogen in the metal, and nickel or platinum can hold hydrogen on the surface, and the present invention provides a composition using such a hydrogen storage metal.

Among the remaining metals after hydrogen is liberated, palladium, nickel, and platinum may be undesirable from the viewpoint of safety because metals insoluble in water remain in the body. Therefore, magnesium hydride (generally represented by the formula of MgH ₂ ) can be obtained from the following reaction formula. Likewise, it can react with water to generate molecular hydrogen, and since magnesium, which has been confirmed to be safe in the body, remains, it is most preferable as a metal containing hydrogen for oral administration.

<Reaction Scheme>

MgH ₂ +2H ₂ O → Mg(OH) ₂ +2H ₂

As long as the technology for preparing the composition of the present invention is widely known in the technical field to which the present invention pertains, it is used without particular limitation and can be sufficiently manufactured by those skilled in the art.

In addition, in order to prolong the generation time of hydrogen in a single dose, when preparing granules, gastric granules and enteric granules can be prepared and mixed and consumed as a single dose granule. Or, you can make capsules or tablets by tableting.

When ingesting such a composition, gastric granules act primarily in the stomach to generate hydrogen, and when the ingested water goes to the intestine, enteric granules act to generate hydrogen, making it possible to prolong the time for hydrogen generation. .

The liver protective effect according to the present invention as described above is g-GT (gamma-glutamyl phosphatase), GPT (glutamate-pyruvate trabsminase), GOT (glutamate-oxaloacetlate transminase), AP (alkaline phosphatase) and TBIL (total bilirubin), It may be characterized by lowering at least one indicator of liver damage selected from the group consisting of direct bilirubin (DBIL), and the hangover relief effect is 1 selected from blood alcohol concentration, acetaldehyde concentration, respiratory alcohol concentration, and blood alcohol concentration. It may be characterized by lowering a hangover relieving index factor or increasing the activity of aldehyde dehydrogenase.

The dosage of the composition of the present invention will vary depending on the age, sex, and weight of the subject to be treated, the specific disease or pathology to be treated, the severity of the disease or pathology, the route of administration, and the judgment of the prescriber. Dosage determination based on these factors is within the level of one of skill in the art, and dosages generally range from 0.01 mg/kg/day to approximately 2,000 mg/kg/day.

A more preferred dosage is between 1 mg/kg/day and 500 mg/kg/day. Administration may be administered once a day, or may be divided several times. The above dosage does not in any way limit the scope of the present invention.

In addition, the mixing weight ratio of the active ingredients constituting the composition of the present invention is not particularly limited, but as a result of various experiments, 50 to 80% by weight of the hydrogen occluding metal, 10 to 25% by weight of vitamin C, gold hwagyu and foam It is preferable that it is 10 to 25% by weight of freeze-dried powder extracted with hot water in the same weight ratio of tree leaves.

The present invention may further include a freeze-dried powder of the green onion sprout hot water extract as another embodiment, in this case, 50 to 80% by weight of the hydrogen occluding metal, 5 to 20% by weight of vitamin C, and Geumhwagyu and It is preferable that the mixture is 2 to 20% by weight of hot water extracted freeze-dried powder and 5 to 20% by weight of hot water extracted freeze-dried green onion sprouts.

The green onion sprout refers to a young sprout grown for about 3 to 10 days after sowing the green onion seeds, and hot water extraction and freeze drying methods can be used without particular limitation as long as they are widely known in the technical field to which the present invention belongs.

Hereinafter, a preferred embodiment of the present invention will be described in detail. The present invention is not limited by the examples described herein and may be sufficiently embodied in other forms. Rather, it is provided to sufficiently convey the spirit of the present invention to those skilled in the art so that the contents introduced herein are thorough and complete.

< Example : Preparation of the composition according to the present invention>

The composition according to the present invention was prepared by mixing magnesium hydride as hydrogen occluding metal in the same weight ratio of magnesium hydride, vitamin C, Geumhwagyu, and phofo tree leaf in the same weight ratio, hot-water extraction freeze-dried powder, and green onion sprout hot-water extraction freeze-dried powder as shown in Table 1. I did.

The freeze-dried powder extracted with hot water in the same weight ratio of Geumhwagyu and Popo tree leaves is prepared as a powder by adding 1kg of Geumhwagyu and Popo tree leaves to 1 liter of water, decoction for about 20 hours, and then freeze-drying them.

The green onion sprout hot-water extract freeze-dried powder is prepared as a powder by adding 1 kg of green onion sprouts 1 week after sowing into 1 liter of water, decoding for about 20 hours, concentrating and freeze-drying them.

weight% Magnesium hydride Green onion sprout Geumhwagyu Popo vitamin Example 1 100 150 150 Example 2 100 150 200 Example 3 100 200 200 Example 4 100 150 150 10 Example 5 100 150 200 10 Example 6 100 200 2000\ 10

< Experimental example : Alcohol administration animal model induction and substance administration>

The experimental animals were Sprague-Dawley male rats with a body weight ranging from 160 to 180 g were purchased from Daehan Biolink (Eumseong, Korea). The breeding environment was maintained at 20±2℃ temperature, 55±1% (RH) humidity, and 12 hour intervals of light and dark cycles and circulated for 1 week.

The feed was given a certain amount of solid feed for experimental animals (PMI Nutrition International) at a certain time every day, and the water was freely ingested using sterile water using a water bottle.

As a control, a solid feed for experimental animals (PMI Nutrition International) was pulverized with a roll mill to a particle size of 70 mesh, then made into granules having a hardness of 30 kg/cm with a counter rotating granulator, and then dried. As a test group, experimental animals For solid feed (PMI Nutrition International), the composition of the example was mixed in a ratio of 5:5 by weight, and then prepared in the same manner as the control group.

The general feed granules prepared in this way and the granules containing the Example composition were fed unlimitedly to experimental animals (8 animals per each group).

At this time, ethanol feeding was administered orally for 6 weeks through a stomach tube at a level of 25(v/v)% ethanol aqueous solution at a level of 5mL/kg and bw/day. In other words, ethanol was not administered for 1 week of the 8-week breeding period to allow the adaptation period to the new mixed powder feed, and after 6 weeks of administration, the last 1 week was not administered.

The test treatment is as shown in Table 2 below, and the results of the subsequent evaluation of the condition of the experimental animals are indicated according to the divisions in Table 2 below.

division Administration group Control General feed granule administration Comparative group 25%(v/v) ethanol aqueous solution, general feed granule administration Example 1 administration group 25% (v/v) ethanol aqueous solution, general feed and Example 1 mixed granule administration Example 2 administration group 25% (v/v) ethanol aqueous solution, general feed and Example 2 mixed granule administration Example 3 administration group 25% (v/v) ethanol aqueous solution, general feed and Example 3 mixed granule administration Example 4 administration group 25% (v/v) ethanol aqueous solution, general feed and Example 4 mixed granule administration Example 5 administration group 25% (v/v) ethanol aqueous solution, general feed and Example 5 mixed granule administration Example 6 Administration group 25% (v/v) ethanol aqueous solution, general feed and Example 6 mixed granule administration

<Check weight change>

Symptoms were observed once a day for all animals of the experimental example. Body weight was measured using an animal weight scale every 5 days at a certain time, and the results are shown in Table 3.

division start 5 10 15 20 25 30 Control 169g 175g 212g 227g 232g 240g 255g Comparative group 168g 170g 181g 192g 209g 217g 221g Example 1 administration group 169g 173g 207g 222g 228g 234g 250g Example 2 administration group 170g 174g 211g 225g 229g 237g 252g Run 3 administration group 170g 174g 210g 225g 230g 238g 253g Example 4 administration group 171g 174g 210g 225g 230g 237g 253g Example 5 administration group 171g 175g 213g 226g 232g 241g 268g Example 6 Administration group 171g 175g 211g 226g 231g 241g 268g

No animals died during this test period, and no animals with specific clinical symptoms were observed. As shown in Table 3, the body weight overall increased in all groups during the test period, but the group of the example showed almost the same increase as that of the control group, but the degree of slowing was very severe in the case of the comparison group.

<Blood biochemical analysis results>

After the test was completed, the blood collected from the abdominal artery was allowed to stand at room temperature for 30 minutes, then centrifuged at 4℃ for 15 minutes at 3,000 rpm to separate the serum, and then use the serum enzyme in an automatic serum analyzer (Dri-chem 2000, Fujifilm, Tokyo). , Japan). The types of serum enzymes are glutamate-pyruvate (GPT), glutamate-oxaloacetlate (GOT), g-GT (gammaphosphatase), alkaline phosphatase (AP), total bilirubin (TBIL) and direct-bilirubin (DBIL). And shown in the table below.

As enzymes used to diagnose liver disease, GOT, GPT, g-GT and ALP tests are widely used. The increase in the activity of these enzymes has a relatively high correlation with the degree of cell disorder and fluctuates more sensitively than other blood enzymes.

division GOT(U/L) GPT(U/L) Control 75.4±3.4 50.2±3.6 Comparative group 121.2±3.4 85.4±3.1 Example 1 administration group 88.4±3.1 73.1±2.7 Example 2 administration group 84.4±4.4 70.2±4.2 Example 3 administration group 78.2±4.1 59.1±4.4 Example 4 administration group 81.3±3.4 62.8±3.2 Example 5 administration group 71.3±3.2 54.1±2.4 Example 6 Administration group 71.5±3.9 54.3±3.4

Looking at Table 4, it was confirmed that the example administration group significantly decreased compared to the comparative group.

In addition, Alkaline phosphatase (AP) activity is shown in Table 5 below.

division AP(U/L) Control 150 Comparative group 275 Example 1 administration group 183 Example 2 administration group 181 Example 3 administration group 177 Example 4 administration group 179 Example 5 administration group 156 Example 6 Administration group 157

Referring to Table 5, in the case of AP, compared to the comparative group, the average of 31.2% was improved in the example group of the present invention, showing excellent effects.

The AP level is a value that increases as the damage/recovery cycle of hepatocytes increases, and is an index supporting GPT inhibition, and it was confirmed that the group administered the granules prepared in the Example powder together with alcohol has an effective inhibitory ability. It can be said that the composition of is an indicator supporting the activity of inhibiting GOT or billirubin levels.

Subsequently, if Gamma-glutamyl phosphatase (g-GT) is highly increased, it will be chronic cholestasis, and biliary cirrhosis or sclerosing cholangitis can be estimated and diagnosed.

division g-GT(U/L) Control 5.9 Comparative group 6.6 Example 1 administration group 6.2 Example 2 administration group 6.1 Example 3 administration group 6.2 Example 4 administration group 6.2 Example 5 administration group 5.8 Example 6 administration group 5.7

Referring to Table 6, g-GT was improved by an average of 20.6% in the group to which the Example of the present invention was administered compared to the comparative group, showing excellent effects.

Lastly, when total bilirubin (TBIL) is increased in blood, it can be seen as obstructive jaundice that is not excreted from the liver into the biliary tract. The results of TBIL activity analysis are shown in Table 7.

division TBIL(U/L) Control 0.61 Comparative group 0.75 Example 1 administration group 0.52 Example 2 administration group 0.50 Example 3 administration group 0.51 Example 4 administration group 0.50 Example 5 administration group 0.44 Example 6 Administration group 0.45

Referring to Table 7 above, TBIL was improved by an average of 39% in the group administered the Example of the present invention compared to the comparative group, showing excellent effects.

These results can also be confirmed that the composition of the present invention has liver protective activity.

<Histological analysis results>

The right lobe of the liver tissue of the experimental animal was cut to measure the content of reduced glutathione (GSH) and oxidized glutathione (GSSG) involved in the redox function in the liver tissue.

The cut liver tissue was sliced and weighed, and then 4 times the amount of 01M potassium phosphate buffer (pH 74) was added and ground with a tissue grinder in ice to obtain an enzyme solution containing enzymes. With this enzyme solution, the reduced glutathione content was analyzed according to the method of Ellman (1959) and S'wiergosz-Kowaleska et al. (2006). The subtracted value was used as reduced glutathione, and the values are shown in Table 8.

Reduced glutathione refers to glutathione with an SH (S for sulfur, H for hydrogen) group attached to it. This reduced glutathione plays a role in removing harmful active oxygen.See Table 8, the amount of reduced glutathione increased by 14.5% on average in the Example administration group compared to the comparative group, so that the Example composition more helpful in antioxidant activity. I could confirm that it was.

division Total GSH (μmol) Reduced GSH(μmol) Control 4.66±0.38 3.99±0.35 Comparative group 4.28±0.42 3.32±0.33 Example 1 administration group 4.59±0.24 3.75±0.31 Example 2 administration group 4.57±0.41 3.80±0.42 Example 3 administration group 4.59±0.42 3.85±0.44 Example 4 administration group 4.58±0.32 3.82±0.32 Example 5 administration group 4.68±0.41 3.98±0.35 Example 6 Administration group 4.67±0.33 3.98±0.34

<Inflammation and Oxidative Checking the stress suppression effect>

In the blood of all experimental animals, the inflammatory cytokines TNF-α and IL-1β were measured, and the results are shown in Table 9.

Each experiment was confirmed using a kit (TNF-α IRTA800) provided by R&D systems. To briefly explain this process, the serum obtained in this experiment was reacted for 2 hours in the experiment in a 96 well plate precoated with an antibody capable of binding to each cytokine present in the blood, and then a secondary antibody was coagulated. The absorbance was confirmed at 540 nm or 570 nm by treating a substrate solution with an unknown amount of bound.

division TNF-α (pg/ml) IL-1β (pg/ml) Control 6.1±0.2 131.2±3.5 Comparative group 17.3±1.7 196.3±5.0 Example 1 administration group 8.5±1.3 130.2±2.8 Example 2 administration group 8.3±1.5 127.2±5.2 Example 3 administration group 7.7±0.4 125.1±3.1 Example 4 administration group 7.9±0.8 126.5±4.2 Example 5 administration group 6.5±0.5 122.1±5.2 Example 6 Administration group 6.9±0.4 122.5±4.3

As shown in Table 9, both the expression of TNF-α and IL-1β in the comparative group increased, but in the Example administration group, the expression of these cytokines and markers was significantly reduced close to that of the control group, which was a normal diet group, and thus It was found that production was suppressed.

From the above results, it can be seen that the composition of the present invention has very excellent liver protection effect, and through this function, a pharmaceutical composition or health functional food for preventing, improving or treating various alcoholic diseases such as hepatitis or fatty liver caused by alcohol It is commercially available.

<Evaluation of blood alcohol reduction efficacy>

In order to confirm the alcohol decomposition effect of the composition prepared in the examples, alcohol was administered to the experimental animals, and then the blood alcohol concentration and acetaldehyde concentration were measured after 1, 3, and 5 hours to evaluate the alcohol decomposition effect in the body. The number of experimental animals was 6 per group, and the classification of each animal experimental group followed the notation in Table 2 above.

Alcohol was administered orally through a stomach tube of 25 (v/v)% ethanol aqueous solution at a level of 3 g/kg and bw/day, and each sample was prepared by using 500 mg/kg of the granule preparation powder of the example in the same amount as the alcohol dose. It was dispersed in distilled water and administered twice at 30 minutes before and after alcohol administration.

After administration, blood alcohol concentration and acetaldehyde concentration were measured 1 and 5 hours after administration to evaluate the alcohol decomposition effect in the body. As for the measurement method, blood was collected from the abdominal vein of an experimental animal, and the concentration of alcohol in the serum obtained by centrifuging the blood collected at 3000 rpm for 30 minutes was measured using an ethanol measurement kit (abcam AB6543), and is shown in Table 10 below.

Blood alcohol concentration (mg% alcohol) Blood acetaldehyde concentration (mg/L) One 5 One 5 Comparative group 203.2±4.1 148.2±4.5 0.258 0.132 Example 1 administration group 92.4±5.1 77.2±3.5 0.214 0.88 Example 2 administration group 91.5±3.5 74.8±2.9 0.214 0.85 Example 3 administration group 90.1±4.1 64.8±3.3 0.210 0.77 Example 4 administration group 91.1±4.5 70.1±4.3 0.213 0.80 Example 5 administration group 87.4±4.1 56.1±4.1 0.157 0.51 Example 6 Administration group 88.1±4.4 57.2±4.5 0.165 0.53

Referring to Table 10, the blood alcohol concentration and acetaldehyde concentration according to the alcohol administration of the comparative group decreased with time, but the degree of reduction was gentle. On the other hand, it was confirmed that in the group to which the Example composition was administered at the same time as the alcohol administration, the blood alcohol concentration was significantly lowered compared to the comparative group, and was also remarkably excellent in the reduction of acetaldehyde concentration.

As such, it was confirmed that the composition of the present invention has an effect of remarkably reducing the concentration of ethanol and acetaldehyde in blood by maximizing the hangover relief ability.

<Aldehyde dehydrogenase ( ALDH ) Active and acetaldehyde ( ADH ) Investigation of amount generated>

In order to investigate the effect of the composition of the above example on the aldehyde dehydrogenase (ALDH) activity and the amount of acetaldehyde (AD) produced, the ALDH activity and the content of ADH were measured using the serum of the experimental animal obtained in Experimental Example 1. ALDH activity was measured using an aldehyde dehydrogenase activity colorimetric assay kit (BioVision), and ADH was measured using an aldehyde quantification assay kit, and the values are shown in the table below. It is shown in 11.

ALDH activity (mU) ADH(mM/L) One 5 One 5 Comparative group 1.4±0.8 1.1±0.3 115±8.2 147±8.7 Example 1 administration group 1.8±0.2 1.4±0.3 105±8.2 127±8.4 Example 2 administration group 1.8±0.5 1.5±0.1 101±8.7 121±8.4 Example 3 administration group 1.9±0.2 1.8±0.4 94.5±7.2 104±8.1 Example 4 administration group 1.9±0.2 1.8±0.1 97.2±8.7 112±8.5 Example 5 administration group 2.1±0.5 2.2±0.4 91.2±8.5 99.2±5.7 Example 6 Administration group 2.1±0.3 2.1±0.2 91.5±8.2 100.3±5.9

As shown in Table 11, the activity of aldehyde dehydrogenase was significantly higher in the Example administration group than in the comparative group, and the amount of acetaldehyde was significantly reduced, showing excellent effects.

Therefore, it was found that the composition of the present invention has excellent alcohol resolution.

<Check the hangover relief effect>

In order to check whether the composition of the present invention actually relieves hangover the next day of drinking, the composition of the present invention was taken after drinking.

Each group was tested with 10 healthy men and women over 20 years of age, and for the experiment, subjects were asked to eat three pieces of samgyeopsal per glass of soju and drink 300 ml of soju over 60 minutes. Each of the granules prepared from the composition of the example was ingested by 4 g. Comparative group 1 was to take 300 ml of hydrogen water in which 0.4 ppm of hydrogen was dissolved, and comparative group 2 was to take two commercially available morning care bottles (200 ml), and 300 ml of mineral water was used as a control group.

The items on the hangover elimination effect, digestive effect, and headache degree checked by these groups the next morning were set to 1 for'no effect' and 5 for'highest effect', within the range of step 5 (1-5). Created and shown in Table 12 below.

Hangover removal effect Degree of digestion Degree of headache reduction Control 1.1 1.2 1.1 Comparative group 1 3.1 3.1 3.0 Control group 2 2.8 2.9 3.1 Example 1 3.7 3.6 3.7 Example 2 3.9 3.8 4.1 Example 3 4.4 4.3 4.5 Example 4 4.1 4.1 4.1 Example 5 4.5 4.6 4.7 Example 6 4.5 4.4 4.7

Referring to Table 12, when taking the composition according to the present invention showed a very high hangover relief effect.

The composition of the present invention as described above has the effect of inhibiting liver damage, can be used as a composition that can prevent or treat various liver diseases by lowering the hepatitis index, and has excellent alcohol decomposition effect and acetaldehyde decomposition effect, and after drinking It is also very useful as a composition for protecting the liver or relieving hangovers taken before/after drinking by relieving headaches and vomiting symptoms the next day.

Claims (6)

Green onion sprout hot water extract freeze-dried powder;
Freeze-dried powder extracted with hot water extracted with the same weight ratio of Geumhwagyu and Popo tree leaves: and
Health functional food composition for the prevention or improvement of liver protection, hangover relief or alcoholic liver disease, characterized in that it contains a hydrogen occlusion metal as an active ingredient.
The health functional food composition for preventing or improving liver protection, hangover relief, or alcoholic liver disease according to claim 1, wherein the hydrogen storage metal is magnesium hydride.
The method of claim 1 or 2, wherein when the hydrogen-occluding metal is 100 in a weight ratio, green onion sprout hot water extraction freeze-dried powder 150 to 200, and Geumhwagyu and Popola leaf same weight ratio mixture hot water extraction freeze-dried powder 150 to 200 Health functional food composition for the prevention or improvement of liver protection, hangover relief or alcoholic liver disease, characterized in that.
[3] The health functional food composition according to claim 1 or 2, wherein the composition further comprises vitamin C for preventing or improving liver protection, hangover relief, or alcoholic liver disease.
The method according to claim 4, wherein when the hydrogen-occluding metal is 100 in weight ratio, green onion sprout hot water extracted freeze-dried powder 150 to 200, Geumhwagyu and Popo tree leaf in the same weight ratio mixture hot water extracted freeze-dried powder 150 to 200, vitamin C 10 Health functional food composition for the prevention or improvement of liver protection, hangover relief or alcoholic liver disease, characterized in that to 20.
[3] The health functional food composition of claim 1 or 2, wherein the alcoholic liver disease is alcoholic hepatitis, alcoholic fatty liver or alcoholic liver cirrhosis.