LT3728583T - Neigiama atranka, slopinant sąlygiškai esminius genus - Google Patents

Neigiama atranka, slopinant sąlygiškai esminius genus

Info

Publication number
LT3728583T
LT3728583T LTEPPCT/EP2018/084463T LTEP2018084463T LT3728583T LT 3728583 T LT3728583 T LT 3728583T LT EP2018084463 T LTEP2018084463 T LT EP2018084463T LT 3728583 T LT3728583 T LT 3728583T
Authority
LT
Lithuania
Prior art keywords
inhibition
counter
selection
essential genes
conditionally essential
Prior art date
Application number
LTEPPCT/EP2018/084463T
Other languages
English (en)
Inventor
Michael Rasmussen
Poul Pedersen
Original Assignee
Novozymes A/S
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes A/S filed Critical Novozymes A/S
Publication of LT3728583T publication Critical patent/LT3728583T/lt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
LTEPPCT/EP2018/084463T 2017-12-22 2018-12-12 Neigiama atranka, slopinant sąlygiškai esminius genus LT3728583T (lt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17210256 2017-12-22
PCT/EP2018/084463 WO2019121192A1 (en) 2017-12-22 2018-12-12 Counter-selection by inhibition of conditionally essential genes

Publications (1)

Publication Number Publication Date
LT3728583T true LT3728583T (lt) 2023-06-12

Family

ID=60957052

Family Applications (1)

Application Number Title Priority Date Filing Date
LTEPPCT/EP2018/084463T LT3728583T (lt) 2017-12-22 2018-12-12 Neigiama atranka, slopinant sąlygiškai esminius genus

Country Status (7)

Country Link
US (1) US20210017544A1 (lt)
EP (1) EP3728583B1 (lt)
CN (1) CN111630165A (lt)
DK (1) DK3728583T3 (lt)
FI (1) FI3728583T3 (lt)
LT (1) LT3728583T (lt)
WO (1) WO2019121192A1 (lt)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112592881B (zh) * 2021-02-25 2021-06-11 中国科学院天津工业生物技术研究所 用于高效外源蛋白表达和高密度培养的工程枯草芽孢杆菌

Family Cites Families (32)

* Cited by examiner, † Cited by third party
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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
DK639689D0 (da) 1989-12-18 1989-12-18 Novo Nordisk As Indfoering af dna i celler
DK0667910T3 (da) 1991-11-14 2003-12-01 Novozymes As En Bacillus-promoter, afledt fra en variant af en alpha-amylase-promoter fra Bacillus licheniformis
DK153992D0 (da) 1992-12-22 1992-12-22 Novo Nordisk As Metode
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
BR9507817A (pt) 1994-06-03 1997-09-16 Novo Nordisk Biotech Inc Construção de dna enzima vetor recombinante célula hospedeira recombinante lacase de ascomicete ou deuteromicete processos para obter uma enzima de lacase para melhorar o rendimento de enzima recombinante para polimerizar um substrato de lignina ou lignossulfato em soluç o para despolimerizar in situ a pasta kraft para oxidar corantes ou precursores de corantes para pintar cabelo e para polimerizar ou oxidar um composto fenólico ou anilina composição de corante e recipiente contendo a mesma
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
DK0805867T3 (da) 1995-01-23 2004-04-13 Novozymes As DNA integration ved transposition
AU5001496A (en) 1995-03-22 1996-10-08 Novo Nordisk A/S Introduction of dna into bacillus strains by conjugation
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
DE69932345T2 (de) 1998-10-26 2007-07-19 Novozymes A/S Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
WO2000056900A2 (en) 1999-03-22 2000-09-28 Novo Nordisk Biotech, Inc. Promoter sequences derived from fusarium venenatum and uses thereof
AU2001265835A1 (en) 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
EP1687433A1 (en) 2003-10-31 2006-08-09 Novozymes A/S Method for stable gene-amplification in a bacterial host cell
CA2563173A1 (en) 2004-03-31 2005-10-20 Novozymes Biopolymer A/S Methods for producing hyaluronic acid in a bacillus cell
WO2006042548A1 (en) 2004-10-22 2006-04-27 Novozymes A/S Stable genomic integration of multiple polynucleotide copies
CN101495625B (zh) 2006-05-31 2012-11-14 诺维信公司 地衣芽孢杆菌中的氯霉素抗性选择
EP2210898A1 (en) 2006-11-29 2010-07-28 Novozymes Inc. Bacillus Licheniformis chromosome
WO2008067423A2 (en) 2006-11-29 2008-06-05 Novozymes, Inc. Methods of improving the introduction of dna into bacterial cells
JP2012504390A (ja) 2008-09-30 2012-02-23 ノボザイムス，インコーポレイティド 糸状菌細胞におけるポジティブ及びネガティブ選択遺伝子の使用方法
WO2014052630A1 (en) 2012-09-27 2014-04-03 Novozymes, Inc. Bacterial mutants with improved transformation efficiency
WO2015065964A1 (en) * 2013-10-28 2015-05-07 The Broad Institute Inc. Functional genomics using crispr-cas systems, compositions, methods, screens and applications thereof
PL3152312T3 (pl) * 2014-06-06 2020-08-10 Regeneron Pharmaceuticals, Inc. Sposoby i kompozycje do modyfikowania docelowego locus
US11254933B2 (en) * 2014-07-14 2022-02-22 The Regents Of The University Of California CRISPR/Cas transcriptional modulation
US10676726B2 (en) * 2015-02-09 2020-06-09 Duke University Compositions and methods for epigenome editing
WO2016205745A2 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Cell sorting
CN108473986A (zh) * 2016-01-08 2018-08-31 诺维信公司 芽孢杆菌宿主细胞的基因组编辑
US11674145B2 (en) * 2016-01-25 2023-06-13 The Regents Of The University Of California Pathway integration and expression in host cells
WO2017136629A1 (en) * 2016-02-05 2017-08-10 Regents Of The University Of Minnesota Vectors and system for modulating gene expression
WO2017191210A1 (en) * 2016-05-04 2017-11-09 Novozymes A/S Genome editing by crispr-cas9 in filamentous fungal host cells
WO2018077796A1 (en) 2016-10-25 2018-05-03 Novozymes A/S Flp-mediated genomic integrationin bacillus licheniformis

Also Published As

Publication number Publication date
EP3728583B1 (en) 2023-03-01
US20210017544A1 (en) 2021-01-21
DK3728583T3 (da) 2023-05-30
CN111630165A (zh) 2020-09-04
FI3728583T3 (fi) 2023-05-11
EP3728583A1 (en) 2020-10-28
WO2019121192A1 (en) 2019-06-27

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