KR970065724A - 폴리펩티드의 제조방법 - Google Patents

폴리펩티드의 제조방법 Download PDF

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KR970065724A
KR970065724A KR1019970033891A KR19970033891A KR970065724A KR 970065724 A KR970065724 A KR 970065724A KR 1019970033891 A KR1019970033891 A KR 1019970033891A KR 19970033891 A KR19970033891 A KR 19970033891A KR 970065724 A KR970065724 A KR 970065724A
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seq
amino acid
precursor
chromatography
interleukin
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KR1019970033891A
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KR100496001B1 (ko
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다다오 다니모도
마사시 구리모토
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하야시바라 겐
가부시키가이샤 하야시바라 세부쓰 가가쿠 겐쿠쇼
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C12N9/6475—Interleukin 1-beta convertase-like enzymes (3.4.22.10; 3.4.22.36; 3.4.22.63)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products

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  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
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Abstract

인터로이킨-1β변환요소와 면역담당 세포에서 인터페론-γ생성을 유도하는 폴리펩티드의 선구물을 접촉시켜 상기 선구물을 활성의 상기 폴리펩티드로 변환하는 단계를 포함하는 것을 특징으로 하는 면역담당 세포에서 인터페론-γ생성을 유도하는 폴리펩티드 선구물의 변환방법.

Description

폴리 펩티드의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. 인터로이킨-1β변환효소를 면역담당 세포에서 인터페론-γ생성을 유도하는 폴리펩티드의 선구물과 접촉시켜 상기 선구물을 활성 폴리펩티드로 변환하는 단계를 포함하는 것을 특징으로 하는 면역담당 세포에서 인터페론-γ생성을 유도하는 활성 폴리펩티드의 제조방법.
  2. 청구항 1에 있어서, 상기 선구물은 N 말단 영역에서 서열번호(SEQ ID NO:)1의 아미노산 서열의 일부 또는 전부를 포함하고, 상기 인터로이킨-1β변환효소는 서열번호(SEQ ID NO:)1의 아미노산 서열에서의 아스파르트산 36과 티로신 37사이의 결합을 절단하는 것을 특징으로 하는 제조방법:
    서열번호(SEQ ID NO:)1:
  3. 청구항 1에 있어서, 상기 선구물은 서열번호(SEQ ID NO:)2(여기서 부호 “Xaa”는 아미노산으로서 “이소로이신” 또는 “트레오닌”임)의 아미노산 서열을 가지는 제조방법:
    서열번호(SEQ ID NO:)2:
  4. 청구항 1에 있어서, 상기 활성 폴리펩티드는 N말단 영역에서 서열번호(SEQ ID NO:)3의 아미노산 서열을 가지는 제조방법:
    서열번호(SEQ ID NO:)3:
  5. 청구항 1에 있어서, 상기 활성 폴리펩티드는 서열번호(SEQ ID NO:)4(여기서 부호“Xaa”는 아미노산 으로서 “이소로이신” 또는 “트레오닌”임)의 아미노산 서열을 가지는 제조방법:
    서열번호(SEQ ID NO:)4:
  6. 청구항 1에 있어서, 상기 선구물을 등몰량 또는 그 이하의 양의 상기 인터로이킨-1β변환효소와 4∼40℃의 온도에서 접촉시키는 제조방법.
  7. 청구항 1에 있어서, 상기 선구물을 등몰량 또는 그 이하의 양의 상기 인터로이킨-1β변환효소와 6∼9의 pH에서 접촉시키는 제조방법.
  8. 청구항 1에 있어서, 상기 인터로이킨 -1β변환효소를 환원제 존재하에서 상기 선구물에 작용시키는 제조 방법.
  9. 청구항 8에 있어서, 상기 환원제는 2-메르캅토에탄올, 디티오드레이톨 및 환원 글루타티온으로 된 군으로부터 선택되는 1종 이상인 제조방법.
  10. 청구항 1에 있어서, 상기 활성 폴리펩티드를 염석, 투석, 여과, 농축,분별 침전, 이온교환 크로마토그래피, 겔 여과 크로마토그래피, 흡착 크로마토그래피, 등전 크로마토그래피, 소수 크로마토그래피, 역상 크로마토그래피, 친화성 크로마토그래피, 겔 전기영동 및 등전점 분획으로 된 군으로부터 선택되는 1종 이상의 기술에 의해 채취하는 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019970033891A 1996-07-25 1997-07-19 폴리펩티드의제조방법 KR100496001B1 (ko)

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JP96-213267 1996-07-25
JP21326796 1996-07-25
JP3147497 1997-01-31
JP97-31474 1997-01-31

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KR970065724A true KR970065724A (ko) 1997-10-13
KR100496001B1 KR100496001B1 (ko) 2005-09-30

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US (1) US5891663A (ko)
EP (1) EP0821005A3 (ko)
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TW (1) TW515843B (ko)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH1080270A (ja) * 1996-07-19 1998-03-31 Hayashibara Biochem Lab Inc ポリペプチドをプロセッシングする酵素
CA2324168A1 (en) * 1999-11-16 2001-05-16 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Antibody specific to interleukin 18 precursor
EP2332579A3 (en) * 2000-02-10 2011-09-21 Abbott Laboratories Antibodies that bind human interleukin-18 and methods of making and using
US7186528B2 (en) * 2000-06-15 2007-03-06 Smithkline Beecham Corporation Method for preparing a physiologically active IL-18 polypeptide
PL376536A1 (pl) 2002-08-28 2006-01-09 Immunex Corporation Kompozycje i sposoby leczenia chorób układu sercowo-naczyniowego
DE602004025799D1 (de) 2003-04-15 2010-04-15 Glaxosmithkline Llc Humane il-18 substitutionsmutanten und deren konjugate
US7968684B2 (en) 2003-11-12 2011-06-28 Abbott Laboratories IL-18 binding proteins

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GB2016015B (en) * 1978-01-22 1982-05-06 Hayashibara Co Method of preparing interferon and preparations containing interferon
JPS5654158A (en) * 1979-10-09 1981-05-14 Hitachi Ltd Control system for call waiting
DE69229252T2 (de) * 1991-08-16 1999-12-16 Merck & Co Inc DNS, welche das Interleukin-1B-Vorläufer-Converting-Enzym kodiert
AU5102993A (en) * 1992-09-18 1994-04-12 Merck & Co., Inc. Dna encoding murine precursor interleukin 1beta converting enzyme
JP3109018B2 (ja) * 1994-07-14 2000-11-13 株式会社林原生物化学研究所 インターフェロン−γの産生を誘導する蛋白質
DE69519454T2 (de) * 1994-07-14 2001-05-03 Hayashibara Biochem Lab Protein, das Interferon-Gamma Herstellung induziert und monoklonaler Antikörper dagegen
JP2952750B2 (ja) * 1995-02-23 1999-09-27 株式会社林原生物化学研究所 モノクローナル抗体
JP2724987B2 (ja) * 1994-11-15 1998-03-09 株式会社林原生物化学研究所 インターフェロン−γの産生を誘導するポリペプチド
TW581771B (en) * 1994-11-15 2004-04-01 Hayashibara Biochem Lab Recombinant production of a polypeptide for inducing interferon-gamma production, and monoclonal antibody against the polypeptide
JPH1080270A (ja) * 1996-07-19 1998-03-31 Hayashibara Biochem Lab Inc ポリペプチドをプロセッシングする酵素

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US5891663A (en) 1999-04-06
EP0821005A3 (en) 1999-10-13
TW515843B (en) 2003-01-01
EP0821005A2 (en) 1998-01-28

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