KR970062692A - 형광방출분광기법을 이용한 수두 바이러스 항체의 역가 측정방법 - Google Patents

형광방출분광기법을 이용한 수두 바이러스 항체의 역가 측정방법 Download PDF

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KR970062692A
KR970062692A KR1019960002533A KR19960002533A KR970062692A KR 970062692 A KR970062692 A KR 970062692A KR 1019960002533 A KR1019960002533 A KR 1019960002533A KR 19960002533 A KR19960002533 A KR 19960002533A KR 970062692 A KR970062692 A KR 970062692A
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South Korea
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measuring
antibody
titers
fluorescence emission
emission spectroscopy
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KR1019960002533A
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KR0161222B1 (ko
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박주홍
김정민
박복련
한윤정
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성재갑
주식회사 Lg 화학
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

본 발명은 수두 바이러스(VZV) 항체의 역가 측정방법에 관한 것으로, 수두 바이러스에 대한 항체를 정량하는데 있어서, 수두 바이러스 항체를 함유하는 시험대상 혈청을 수두 바이러스 항원성 물질과 반응시키고, 상기 반응물에 형광 표지된 염소의 사람에 대한 항체를 반응시키고, 생성된 반응물에 대하여 형광계를 사용하여 방출 스펙트럼을 측정하는 방법에 의하면 신뢰성, 편의성, 재현성 및 민감도가 개선된 VZV항체 역가 측정이 가능하다.

Description

형광방출분광기법을 이용한 수두 바이러스 항체의 역가 측정방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 각각 수두 바이러스 항체의 혈청양성 및 혈청음성 혈청에 대하여 형광계를 사용하여 측정한 방출 스펙트럼을 나타낸 것이다.

Claims (2)

  1. 수두 바이러스에 대한 항체의 역가를 측정하는데 있어서, 수두 바이러스 항체를 함유하는 시험대상 혈청을 수두 바이러스 항원성 물질과 반응시키고, 상기 반응물에 형광 표지된 염소의 사람에 대한 항체(fluorescein-conjugated goat antihuman lgG)를 반응시키고, 생성된 반응물에 대하여 형광계를 사용하여 방출 스펙트럼(emission spectrum)을 측정하는 것을 특징으로 하는 수두 바이러스 항체의 정량적 역가 측정 방법.
  2. 제1항에 있어서, 500 내지 600nm의 방출 파장에서 형광 세기를 측정하는 것을 특징으로 하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960002533A 1996-02-02 1996-02-02 형광방출분광기법을 이용한 수두 바이러스 항체의 역가 측정방법 KR0161222B1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019960002533A KR0161222B1 (ko) 1996-02-02 1996-02-02 형광방출분광기법을 이용한 수두 바이러스 항체의 역가 측정방법

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019960002533A KR0161222B1 (ko) 1996-02-02 1996-02-02 형광방출분광기법을 이용한 수두 바이러스 항체의 역가 측정방법

Publications (2)

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KR970062692A true KR970062692A (ko) 1997-09-12
KR0161222B1 KR0161222B1 (ko) 1999-03-30

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KR1019960002533A KR0161222B1 (ko) 1996-02-02 1996-02-02 형광방출분광기법을 이용한 수두 바이러스 항체의 역가 측정방법

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210149435A (ko) * 2020-06-02 2021-12-09 경북대학교 산학협력단 자가면역질환 진단을 위한 항핵항체 역가측정 방법

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20210149435A (ko) * 2020-06-02 2021-12-09 경북대학교 산학협력단 자가면역질환 진단을 위한 항핵항체 역가측정 방법

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