KR960029789A - 생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법 - Google Patents

생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법 Download PDF

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KR960029789A
KR960029789A KR1019960001537A KR19960001537A KR960029789A KR 960029789 A KR960029789 A KR 960029789A KR 1019960001537 A KR1019960001537 A KR 1019960001537A KR 19960001537 A KR19960001537 A KR 19960001537A KR 960029789 A KR960029789 A KR 960029789A
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magnetic particles
analyte
immobilized
substance
sample
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KR1019960001537A
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요지 다카하시
히사카 고바야시
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후지이 히로시
니폰 페인트 가부시키가이샤
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Publication of KR960029789A publication Critical patent/KR960029789A/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
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  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Investigating Or Analyzing Materials By The Use Of Magnetic Means (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

본 발명은 프로존 현상을 유도하지 않고서 생체내 물질의 편리한 분석이 가능한 면역분석 키트 및 면역분석 방법을 제공한다. 면역분석 키트는 분석 물질에 면역학적으로 결합하는 물질로 고정화시킨 자기성 입자(A), 및 분석 물질로 고정화시킨 비자기성 입자(B)를 적어도 함유한다. 면역분석 키트는 샘플 중의 분석 방해 물질에 면역학적으로 결합하는 물질로 고정화시킨 자기성 입자(C)를 추가로 함유할 수 있다. 샘플을 입자(A) 및 (C)와, 입자(B)와 반응시키고; 반응하지 못한 면역복합체 형태인 자기성 입자를 수집하고; 반응ㆍ용액에 남은 비자기성 입자의 흡광도를 측정함으로써 본 발명의 면역분석 방법을 수행한다. 샘플 중의 분석 물질은 분석 물질의 표준 샘플을 사용하여 제조된 보정 곡선을 참조로 하여 정량적으로 측정한다.

Description

생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제2도는 샘플 중의 분석물질 항원, 및 항원으로 고정화시킨 비자기성 입자가 경쟁적으로 반응하는 본 발명의 면역분석의 공정을 나타내는 도식이다.

Claims (4)

  1. 분석 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자, 및 분석 물질로 고정화시킨, 특정 범위의 흡수 파장을 갖는 불용성 비자기성 입자를 함유함을 특징으로 하여 항원－항체 반응을 이용하여 샘플 중의 분석용 생체내물질을 면역학적으로 분석하는 키트.
  2. 제1항에 있어서, 분석을 방해하는 샘플 중의 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자를 추가로 함유하는 분석용 생체내 물질을 면역학적으로 분석하는 키트.
  3. (1) 분석 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자의 분산액 및 분석 물질로 고정화시킨, 특정 범위의 흡수 파장을 갖는 불용성 비자기성 입자의 분산액을 임의의 순서로 샘플과 혼합하여 혼합물을 제조하고; (2) 상기 혼합물에 자기장을 적용하여 면역학적으로 결합하지 못한 자기성 입자와 함께 상기 혼합물 중에 형성된 상기 자기성 입자와 상기 분석 물질의 복합체를 제거하고; (3) 흡광도를 측정함으로써 혼합물에 남은 상기 비자기성 입자의 농도를 측정하는 단계를 포함함을 특징으로 하여, 항원－항체 반응을 이용하여 샘플 중의 분석용 생체내 물질을 분석하는 방법.
  4. 제3항에 있어서, 분석을 방해하는 샘플 중의 물질에 면역학적으로 결합하는 물질로 고정화시킨 불용성 자기성 입자를 상기 혼합물에 추가로 가하고; 상기 혼합물 중에 형성된 상기 자기성 입자 및 상기 분석 방해물질의 복합체를 자기장에 의해 추가로 제거함을 특징으로 하여 샘플 중의 분석용 생체내 물질을 분석하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019960001537A 1995-01-26 1996-01-24 생체내 물질의 면역학적 분석용 키트 및 면역학적 분석 방법 KR960029789A (ko)

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JP10627/1995 1995-01-26
JP1062795 1995-01-26

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KR960029789A true KR960029789A (ko) 1996-08-17

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EP (1) EP0724156A1 (ko)
KR (1) KR960029789A (ko)
AU (1) AU4213396A (ko)
CA (1) CA2168156A1 (ko)

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US10520521B2 (en) 2014-06-30 2019-12-31 Phc Holdings Corporation Substrate for sample analysis, sample analysis device, sample analysis system, and program for sample analysis system
JP6548645B2 (ja) 2014-06-30 2019-07-24 Ｐｈｃホールディングス株式会社 試料分析用基板および試料分析装置
US10539582B2 (en) 2014-06-30 2020-01-21 Phc Holdings Corporation Substrate for sample analysis, sample analysis device, sample analysis system, and method for removing liquid from liquid that contains magnetic particles
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Publication number Priority date Publication date Assignee Title
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CA2168156A1 (en) 1996-07-27
EP0724156A1 (en) 1996-07-31
AU4213396A (en) 1996-08-01

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