KR970059273A - Antibacterial microorganism preparation and method for producing the same - Google Patents

Abstract

The present invention discloses an antimicrobial microorganism preparation and a preparation method thereof. The antimicrobial microorganism preparation of the present invention contains any one of CYK 10 and WYE 117, which are strains of the novel antimicrobial Streptomyces genus, and can be prepared into a powder form and a liquid form. In the case of powdery microorganism preparations, sawdust, chitin powder, chitosan, laminarin, carboxymethylcellulose and cellobiose are included. In the case of liquid microbial preparations, paclitaxel, alginic acid, colloidal chitin, chitosan, laminarin, carboxymethylcellulose, glycerol , And ammonium sulfate. The antimicrobial microorganism preparation of the present invention is excellent in antibacterial effect due to the novel antimicrobial strain and is very suitable for maintaining the activity of the antimicrobial strain.

Description

Antibacterial microorganism preparation and method for producing the same

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Claims (15)

A microorganism preparation capable of promoting the growth of plants by controlling fungal pathogens of plants includes at least one of Streptomyces moscow WYE 117 (KCTC 0222BP) and Streptomyces sp. CYD 10 (KCTC023B) Wherein the microbial agent is a microbial agent. The microbial preparation according to claim 1, wherein the plant is tomato, ginseng, cucumber, red pepper or lettuce. The method according to claim 1, wherein the microbial agent further comprises a powdered microorganism delivery medium for the strain, wherein the saccharide-type microorganism delivery medium comprises 0.1 to 99.9% by weight of sawdust and chitin powder, chitosan, linalin, carboxymethylcellulose ≪ / RTI > and 0.1 to 99.9% by weight of at least one component selected from the group consisting of: < RTI ID = 0.0 > 4. The method of claim 3, wherein the powdered microorganism delivery medium further comprises 0.1-5% by weight of cellobiose and 0.03-1% by weight of nitrogen source based on the total weight of the transfer medium, and the nitrogen source is ammonium nitrate or ammonium sulfate . The microbial preparation according to claim 3 or 4, wherein the strain contains 10 ⁷ -10 ¹² colony forming units per gram of the powdery microorganism-transferring medium. The method of claim 1, wherein the microbial agent further comprises a liquid microbial delivery vehicle for the strain, wherein the liquid microbial delivery vehicle comprises 0.1-16 wt% of pectin and at least one of alginic acid, colloidal kitchen, chitosan, laminarin, and carboxymethylcellulose 0.5 to 50% by weight of at least one component selected from the group consisting of water and the remainder of water. 7. The method of claim 6, wherein the liquid type microbial delivery vehicle further comprises 0.1-5 wt% glycerol and 0.03-1.0 wt% nitrogen source relative to the total weight of the transfer medium, wherein the nitrogen source is ammonium sulfate or ammonium nitrate Microbial formulation. The microbial preparation according to claim 6 or 7, wherein the strain contains 10 ⁷ -10 ¹² colonies forming units per 1 ml of the liquid type microorganism transfer medium. A method for producing a powdery microorganism preparation comprising an antimicrobial microorganism strain according to any one of claims 1 to 4, which comprises mixing 0.1 to 99.9% by weight of sawdust and at least one component selected from the group consisting of chitin powder, chitosan, laminarin and carboxymethyl cellulose -99.9% by weight of the mixture is further mixed with 0.1-5% by weight of the cellobiose and 0.03-1.0% by weight of ammonium nitrate or ammonium sulfate as a nitrogen source based on the weight of the mixture, Adding and sterilizing the homogenized powdery microorganism delivery medium composition to prepare a homogenous powdery microorganism delivery vehicle composition, mixing the mycelium and microbial cells obtained from the microbial microbial strain with the sterilized powdery microorganism delivery vehicle composition, and freezing the mixture of the microbial strain and the delivery medium ≪ / RTI > wherein the microbial formulation comprises at least one microorganism. [Claim 11] The method according to claim 9, wherein the sterilization is performed by sterilization at 121 DEG C for 1-3 hours and dry sterilization at 160-190 DEG C for 3-12 hours. [Claim 11] The method according to claim 9, wherein the antimicrobial microorganism strain is mixed with 10 ^{7 to} 10 ¹² microorganism strains per gram of the powdery microorganism transfer medium in the mixing process of the microbial strain and the microorganism-transferring medium. 10. The method of claim 9, further comprising packaging the lyophilized microbial formulation after the lyophilization step. A method for producing a liquid type microorganism preparation comprising an antimicrobial microorganism strain according to claim 7, comprising the step of adding 0.1 to 16% by weight of pectin and at least one component selected from the group consisting of alginic acid, colloidal chitin, chitosan, laminarin and carboxymethyl cellulose Ammonium sulphate or ammonium nitrate and the remainder of water is mixed and sterilized as a source of nitrogen of 0.5-50 wt%, glycerol 0.1-5 wt% and 0.03-1.0 wt%, and a homogenous liquid type microorganism delivery medium composition is prepared, And mixing the mycelium and microbial cells obtained from the strain with the sterilized liquid type microorganism delivery vehicle composition. 14. The method according to claim 13, wherein the antimicrobial microorganism strain contains 10 ⁷ -10 ¹² colony forming units per 1 ml of the liquid type microorganism transfer medium. 14. The method of claim 13, wherein the antimicrobial microbial strain further comprises mixing and packaging the liquid type microbicide delivery vehicle composition. ※ Note: It is disclosed by the contents of the first application.