KR970043049A - Method for producing bone-producing protein-1 using yeast - Google Patents

Method for producing bone-producing protein-1 using yeast Download PDF

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Publication number
KR970043049A
KR970043049A KR1019950060930A KR19950060930A KR970043049A KR 970043049 A KR970043049 A KR 970043049A KR 1019950060930 A KR1019950060930 A KR 1019950060930A KR 19950060930 A KR19950060930 A KR 19950060930A KR 970043049 A KR970043049 A KR 970043049A
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KR
South Korea
Prior art keywords
yeast
protein
producing
bone
expression vector
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Application number
KR1019950060930A
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Korean (ko)
Inventor
양재영
이재형
조중명
Original Assignee
성재갑
주식회사 엘지화학
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Application filed by 성재갑, 주식회사 엘지화학 filed Critical 성재갑
Priority to KR1019950060930A priority Critical patent/KR970043049A/en
Publication of KR970043049A publication Critical patent/KR970043049A/en

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Abstract

본 발명은 효모를 이용한 골 생성 단백질-1(Osteogenic Protein-1)의 생산방법에 관한 것으로, 골 생성 단백질-1(OP-1)을 코드하는 cDNA 염기서열이 효모의 알파인자(α-factor)의 전구서열에 연결된 것을 포함하는 효모용 골 생성 단백질-1의 생산을 위한 발현벡터로 형질전환된 효모세포를 배양하여 천연형 이중체 골 생성 단백질-1을 회수하는 것을 포함하는 본 발명에 의해 골 생성 단백질-1을 효율적으로 생성할 수 있다.The present invention relates to a method for producing osteogenic protein-1 (Osteogenic Protein-1) using yeast, the cDNA sequence encoding the osteogenic protein-1 (OP-1) is an alpha factor (α-factor) of the yeast Bone by the present invention comprising culturing the yeast cells transformed with an expression vector for the production of bone producing protein-1 for yeast comprising the one linked to the precursor sequence of The resulting protein-1 can be produced efficiently.

Description

효모를 이용한 골 생성 단백질-1의 제조방법Method for producing bone-producing protein-1 using yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (5)

골 생성 단백질-1(OP-1)을 코드하는 cDNA 염기서열이 효모의 알파인자(α-factor)의 전구서열에 연결된 것을 포함하는 효모용 골생성 단백질-1의 생산을 위한 발현벡터.Expression vector for the production of osteogenic protein-1 for yeast comprising a cDNA sequence encoding the bone production protein-1 (OP-1) is linked to the precursor sequence of the alpha factor (α-factor) of the yeast. 제 1항에 있어서, 발현벡터 pYLBC A/G αF OP-1.The expression vector pYLBC A / G αF OP-1. 제 1항의 발현벡터로 형질전환된 효모세포주.A yeast cell line transformed with the expression vector of claim 1. 제3항에 있어서, 사카로마이세스 세레비지애 pYLBC A/G αF OP-1(KCTC 8711P).4. Saccharomyces cerevisiae pYLBC A / G αF OP-1 (KCTC 8711P). 제 4항의 효모세포를 배양하여 천연형의 이중체 골 생성 단백질-1을 회수하는 것을 포함하는 골 생성 단백질-1의 제조방법.The method for producing bone-producing protein-1 comprising culturing the yeast cells of claim 4 and recovering the native duplex bone-forming protein-1.
KR1019950060930A 1995-12-28 1995-12-28 Method for producing bone-producing protein-1 using yeast KR970043049A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950060930A KR970043049A (en) 1995-12-28 1995-12-28 Method for producing bone-producing protein-1 using yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950060930A KR970043049A (en) 1995-12-28 1995-12-28 Method for producing bone-producing protein-1 using yeast

Publications (1)

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KR970043049A true KR970043049A (en) 1997-07-26

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KR1019950060930A KR970043049A (en) 1995-12-28 1995-12-28 Method for producing bone-producing protein-1 using yeast

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0170000A2 (en) * 1984-06-29 1986-02-05 Chesebrough-Pond's Inc. Nail polish compositions and means for applying same
EP0409472A1 (en) * 1989-07-19 1991-01-23 Chiron Corporation Bone morphogenetic protein
WO1993015197A2 (en) * 1992-01-27 1993-08-05 M.u.r.s.t. Italian Ministry for Universities and Scientific and Technological Research Expression of osteogenic factor op-1 in cells of spodoptera frugiperda infected with recombinant baculovirus

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0170000A2 (en) * 1984-06-29 1986-02-05 Chesebrough-Pond's Inc. Nail polish compositions and means for applying same
EP0409472A1 (en) * 1989-07-19 1991-01-23 Chiron Corporation Bone morphogenetic protein
WO1993015197A2 (en) * 1992-01-27 1993-08-05 M.u.r.s.t. Italian Ministry for Universities and Scientific and Technological Research Expression of osteogenic factor op-1 in cells of spodoptera frugiperda infected with recombinant baculovirus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Growth Factors 1994;11(3):215-25 *

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