KR960031620A - Fermentative production method of low growth human growth hormone - Google Patents

Fermentative production method of low growth human growth hormone Download PDF

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Publication number
KR960031620A
KR960031620A KR1019950002644A KR19950002644A KR960031620A KR 960031620 A KR960031620 A KR 960031620A KR 1019950002644 A KR1019950002644 A KR 1019950002644A KR 19950002644 A KR19950002644 A KR 19950002644A KR 960031620 A KR960031620 A KR 960031620A
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KR
South Korea
Prior art keywords
growth hormone
human growth
production method
fermentation
low
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KR1019950002644A
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Korean (ko)
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KR100274191B1 (en
Inventor
권선훈
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성재갑
주식회사 Lg 화학
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Priority to KR1019950002644A priority Critical patent/KR100274191B1/en
Publication of KR960031620A publication Critical patent/KR960031620A/en
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Publication of KR100274191B1 publication Critical patent/KR100274191B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Endocrinology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 변형체가 적은 인간 성장 호르몬(HGH)의 발효 생산 방법에 관한 것으로, 제조합 효모를 발효시켜 인간 성장 호르몬을 생상하는데 있어서, 단백질 발현 시기에 발효 온도를 15℃ 내지 20℃의 저온으로 전환시키는 것을 특징으로 하는 발효 생산 방법에 의하면 효모로부터 유전자 재조합 인간 성장 호르몬을 80% 이상의 고순도로 얻을 수 있다.The present invention relates to a method for producing fermentation of human growth hormone (HGH) with less strain, and in fermenting a synthetic yeast to produce human growth hormone, converting the fermentation temperature to a low temperature of 15 ° C. to 20 ° C. at the time of protein expression. According to the fermentation production method characterized in that the genetically modified human growth hormone from yeast can be obtained with high purity of 80% or more.

Description

변형체가 적은 인간 성장 호르몬의 발효 생산 방법.Method of fermentation production of human growth hormone with less strain.

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (2)

재조합 효모를 발효시켜 인간 성장 호르몬(HGH)을 생산하는데 있어서, 단백질 발현시기에 발효 온도를 저온으로 전환시키는 것을 특징으로 하는, 변형체가 적은 HGH의 발효 생산 방법.A method for producing fermentation of low variant HGH, characterized in that the fermentation of recombinant yeast to produce human growth hormone (HGH), wherein the fermentation temperature is converted to low temperature at the time of protein expression. 제1항에 있어서, 발효 온도를 15℃ 내지 20℃로 낮추는 것을 특징으로 하는 방법.The method of claim 1, wherein the fermentation temperature is lowered to 15 to 20 ℃. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019950002644A 1995-02-14 1995-02-14 Fermentation process for producing human growth hormone comprising reduced amount of modified hgh KR100274191B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950002644A KR100274191B1 (en) 1995-02-14 1995-02-14 Fermentation process for producing human growth hormone comprising reduced amount of modified hgh

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950002644A KR100274191B1 (en) 1995-02-14 1995-02-14 Fermentation process for producing human growth hormone comprising reduced amount of modified hgh

Publications (2)

Publication Number Publication Date
KR960031620A true KR960031620A (en) 1996-09-17
KR100274191B1 KR100274191B1 (en) 2000-12-15

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ID=19408072

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019950002644A KR100274191B1 (en) 1995-02-14 1995-02-14 Fermentation process for producing human growth hormone comprising reduced amount of modified hgh

Country Status (1)

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KR100274191B1 (en) 2000-12-15

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