KR870700097A - How to prepare ethanol from sucrose - Google Patents

How to prepare ethanol from sucrose

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Publication number
KR870700097A
KR870700097A KR860700660A KR860700660A KR870700097A KR 870700097 A KR870700097 A KR 870700097A KR 860700660 A KR860700660 A KR 860700660A KR 860700660 A KR860700660 A KR 860700660A KR 870700097 A KR870700097 A KR 870700097A
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South Korea
Prior art keywords
sucrose
fermentation
range
zymonas mobilis
concentration
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KR860700660A
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Korean (ko)
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웨르너 도엘레 호르스트
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도날드 제임스 문로
퀸즈랜드 유니버시티
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Publication of KR870700097A publication Critical patent/KR870700097A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/065Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
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    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Tropical Medicine & Parasitology (AREA)
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Abstract

내용 없음.No content.

Description

자당으로 부터 에타놀을 제조하는 방법How to prepare ethanol from sucrose

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (19)

자당이 발효배지에 포함되어 있는 미호기성 상태하에 단일 단계의 공정으로 미생물 자이모모나스 모빌리스로 자당을 발효시키는 것을 특징으로 하는 발효조에서 자당(또는 그 가수분해 생성물 또는 전화당)으로부터 에탄놀을 제조하는 방법.Ethanol is produced from sucrose (or its hydrolyzate or invert sugar) in a fermenter characterized by fermenting sucrose with microbial Zymonas mobilis in a single step process under an aerobic state in which sucrose is contained in the fermentation medium. How to. 제1항에 있어서, 자이모모나스 모빌리스가 기탁번호 제39676호로 ATCC에 기탁된 균주 또는 그 돌연변이체 또는 변종인 것을 특징으로 하는 방법.2. The method of claim 1, wherein the Zymonas mobilis is a strain or a mutant or variant thereof deposited in the ATCC with Accession No. 39676. 제1항에 있어서, 자이모모나스 모빌리스가 기탁번호 제29191호로 ATCC에 기탁된 균주인 것을 특징으로 하는 방법.2. The method of claim 1, wherein Zymonas mobilis is a strain deposited with ATCC under accession no. 제1항에서 제3항중 어느 한항에 있어서, 자당의 정제된 설탕, 원당, 분쇄된 사탕수수즙 사탕무우즙, 당밀 또는(자당 가수분해 생성물 또는 전화당을 함유할 수 있는) 이것들의 둘 또는 그 이상의 조합물의 형태로 발효 배지에 넣는 것을 특징으로 하는 방법.The method according to any one of claims 1 to 3, wherein the refined sugar of sucrose, raw sugar, ground sugar beet juice, molasses or two or more thereof (which may contain sucrose hydrolysis products or invert sugar). Method for placing in the fermentation medium in the form of a combination of the above. 제1항에서 제4항중 어느 한항에 있어서, 자당농도가 10―30%(W/V) 범위에 있는 것을 특징으로 하는 방법.The method of any one of claims 1 to 4, wherein the sucrose concentration is in the range of 10-30% (W / V). 제5항에 있어서, 자당농도가 15―25%(W/V)인 것을 특징으로 하는 방법.6. The method of claim 5, wherein the sucrose concentration is 15-25% (W / V). 제6항에 있어서, 자당농도가 20%(W/V)인 것을 특징으로 하는 방법.7. The method of claim 6, wherein the sucrose concentration is 20% (W / V). 제1항에서 7항중 어느 한항에 있어서, 발효 배지가 펩톤, 이스트 추출물, KH2PO4, (NH4)2SO, 요소 또 MgSO47H2O 중의 하나 또는 그 이상을 포함하고 각 성분의 농도가 0/01―0.5%(W/V) 범위에 있는 것을 특징으로 하는 방법.The method of claim 1, wherein the fermentation medium comprises one or more of peptone, yeast extract, KH 2 PO 4 , (NH 4 ) 2 SO, urea or MgSO 4 7H 2 O and the concentration of each component. Is in the range of 0 / 01-0.5% (W / V). 제8항에 있어서, 각 성분의 농도가 0.2%(W/V)인 것을 특징으로 하는 방법.The method of claim 8 wherein the concentration of each component is 0.2% (W / V). 제1항에서 제9항중 어느 한 항에 있어서, 발효 배지의 pH를 4.0―7.0 범위에서 유지하는 것을 특징으로 하는 방법.10. The method of any one of claims 1-9, wherein the pH of the fermentation medium is maintained in the range of 4.0-7.0. 제11항에 있어서, pH가 초기에는 6.5―7.0 범위에 있고 발효가 시작될 때 pH를 내려가게 하고 그 후에는 5.0-6.0 범위에서 유지하는 것을 특징으로 하는 방법.12. The method of claim 11, wherein the pH is initially in the range 6.5-7.0 and the pH is lowered when fermentation begins and thereafter maintained in the range 5.0-6.0. 제1항에서 제11항중 어느 한항에 있어서, 발효조의 온도를 34℃―40℃의 범위에서 유지하는 것을 특징으로 하는 방법.The method according to any one of claims 1 to 11, wherein the temperature of the fermenter is maintained in the range of 34 ° C to 40 ° C. 제12항에 있어서, 온도를 35℃로 유지하는 것을 특징으로 하는 방법.13. The method of claim 12, wherein the temperature is maintained at 35 ° C. 제1항에서 제13항중 어느 한 항에 있어서, 발효를 끝냈을 때 미생물 자이모모나스 모빌리스를 발효배지로부터 분리하고 에타놀을 증류하는 것을 특징으로 하는 방법.The method of any one of claims 1 to 13, wherein the microbial Zymonas mobilis is separated from the fermentation broth and the ethanol is distilled off when the fermentation is completed. 제1항에 있어서, 자이모모나스 모빌리스가 발효 배지에 부유된 자이모모나스 모빌리스 미생물 세포를 포함하는 것을 특징으로 하는 방법.The method of claim 1 wherein the Zymonas mobilis comprises the Zymonas mobilis microbial cells suspended in the fermentation medium. 제15항에 있어서, 상기 발효의 발효된 배지의 일부를 다음번 발효를 위한 자이모모나스 모빌리스 미생물 세포의 종균으로써 발효조에 넣는 것을 특징으로 하는 방법.16. The method of claim 15, wherein a portion of the fermented medium of the fermentation is placed in a fermenter as a seed of Zymonas mobilis microbial cells for subsequent fermentation. 기탁번호 제39676호로 ATCC에 기탁된 자이모모나스 모빌리스의 균주.Strain of Zymonas mobilis deposited with ATCC under Accession No. 39676. 기탁번호 제39676호로 ATCC에 기탁된 자이모모나스 모빌리스의 균주의 돌연변이체 또는 변종.Mutant or variant of a strain of Zymonas mobilis deposited with ATCC under Accession No. 39676. 제1항에서 제16항중 어느 한 항에 따른 방법에 의해서 자당(또는 그 가수분해 생성물 또는 전화당)으로부터 생성된 에타놀.Ethanol produced from sucrose (or its hydrolysis product or invert sugar) by the process according to claim 1. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR860700660A 1985-01-25 1986-01-24 How to prepare ethanol from sucrose KR870700097A (en)

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Application Number Priority Date Filing Date Title
AUPG9046 1985-01-25
AUPG904685 1985-01-25
AUPG9422 1985-02-22
AUPG942285 1985-02-22
PCT/AU1986/000015 WO1986004357A1 (en) 1985-01-25 1986-01-24 Conversion of sucrose to ethanol using the bacterium zymomonas mobilis

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KR870700097A true KR870700097A (en) 1987-02-28

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AU (1) AU603333B2 (en)
BR (1) BR8606997A (en)
ES (1) ES8702327A1 (en)
GB (1) GB2191503A (en)
MY (1) MY101382A (en)
NL (1) NL8600158A (en)
NZ (1) NZ214933A (en)
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ZW (1) ZW1586A1 (en)

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US4840902A (en) * 1987-05-04 1989-06-20 George Weston Limited Continuous process for ethanol production by bacterial fermentation using pH control
US5000000A (en) * 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
DE10310411A1 (en) * 2003-03-07 2004-10-07 Rch Holding Ag brewery process

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* Cited by examiner, † Cited by third party
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US4350765A (en) * 1979-06-13 1982-09-21 Tanabe Seiyaku Co., Ltd. Method for producing ethanol with immobilized microorganism
AU537029B2 (en) * 1980-05-15 1984-05-31 Unisearch Limited Ethanol production
WO1982001563A1 (en) * 1980-10-23 1982-05-13 Bo Mattiasson Biological and chemical conversion processes in liquid phase-system
AU540186B2 (en) * 1980-12-08 1984-11-08 Unisearch Limited Semi batch ethanol production
AU573742B2 (en) * 1983-09-27 1988-06-23 University Of Queensland, The Conversion of sucrose to fructose and ethanol

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WO1986004357A1 (en) 1986-07-31
GB8716796D0 (en) 1987-08-19
GB2191503A (en) 1987-12-16
ES8702327A1 (en) 1987-01-01
AU603333B2 (en) 1990-11-15
MY101382A (en) 1991-10-23
ZW1586A1 (en) 1986-08-27
NZ214933A (en) 1988-09-29
EP0247044A4 (en) 1988-05-26
AU5397086A (en) 1986-08-13
NL8600158A (en) 1986-08-18
EP0247044A1 (en) 1987-12-02
ES551206A0 (en) 1987-01-01
BR8606997A (en) 1987-12-01

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