KR970042591A - 트레할로스를 안정제로 이용한 단백질용액의 액상가열처리방법 - Google Patents
트레할로스를 안정제로 이용한 단백질용액의 액상가열처리방법 Download PDFInfo
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- KR970042591A KR970042591A KR1019950047617A KR19950047617A KR970042591A KR 970042591 A KR970042591 A KR 970042591A KR 1019950047617 A KR1019950047617 A KR 1019950047617A KR 19950047617 A KR19950047617 A KR 19950047617A KR 970042591 A KR970042591 A KR 970042591A
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Abstract
본 발명은 단백질을 주성분으로 하는 의약품 생산시, 수용액 상태의 시료를 가열처리(섭씨 60℃, 10시간)에 의한 각종 바이러스 불활화시에 트레할로스(D-(+)-Trehalose)를 안정제로 사용하는 액상가열처리방법에 관한 것이다. 트레할로스 농도를 10-100w/v %로 조정하고 pH 5.0-9.0, 섭씨 40-70℃에서 적당한 농도의 단백질을 액상 가열처리시 단백질의 생리활성은 충분히 유지하면서 바이러스를 불화화시킨다. 트레할로스를 안정제로 사용하는 경우 기존의 안정제로서 사용되는 당류에 비해 가열 후에도 단백질의 2차 구조에 가장 적은 변화를 나타내었다.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 안정제로서 트레할로스를 함유한 피브리노겐 용액의 액상가열 처리전과 후의 회전이선광 스펙트럼을 나타낸 것이다.
Claims (4)
- 단백질을 주성분으로 하는 용액의 바이러스 불활화시 안정화제로서 10-100w/v%의 트레할로스를 첨가하여 가열 처리함을 특징으로 하는 단백질용액의 액상가열처리방법.
- 제1항에 있어서, 단백질 용액이 피브리노겐 용액, 면역글로불린 지용액, 혈액응고 제8인자 용액, 혈액응고 제9인자 용액, 혈액응고 제13인자 용액, 유로키나아제 용액, 인뇨 유래 트립신 저해제 용액중에서 선택된 일종임을 특징으로 하는 단백질용액의 액상가열처방법.
- 제1항에 있어서, 첨가되는 트레할로스의 양이 바람직하게는 40-90w/v%임을 특징으로 하는 단백질 용액의 액상가열처리방법.
- 제1항에 있어서, 단백질 용액의 pH가 5.0-9.0 임을 특징으로 하는 단백질용액의 액상가열처리방법.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950047617A KR0182395B1 (ko) | 1995-12-08 | 1995-12-08 | 트레할로스를 안정제로 이용한 단백질용액의 액상가열처리방법 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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KR1019950047617A KR0182395B1 (ko) | 1995-12-08 | 1995-12-08 | 트레할로스를 안정제로 이용한 단백질용액의 액상가열처리방법 |
Publications (2)
Publication Number | Publication Date |
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KR970042591A true KR970042591A (ko) | 1997-07-24 |
KR0182395B1 KR0182395B1 (ko) | 1999-04-01 |
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KR1019950047617A KR0182395B1 (ko) | 1995-12-08 | 1995-12-08 | 트레할로스를 안정제로 이용한 단백질용액의 액상가열처리방법 |
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KR (1) | KR0182395B1 (ko) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100588952B1 (ko) * | 1997-12-09 | 2006-10-13 | 가부시끼가이샤 하야시바라 세이부쓰 가가꾸 겐꾸조 | 혈액순환개선제 |
-
1995
- 1995-12-08 KR KR1019950047617A patent/KR0182395B1/ko not_active IP Right Cessation
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR100588952B1 (ko) * | 1997-12-09 | 2006-10-13 | 가부시끼가이샤 하야시바라 세이부쓰 가가꾸 겐꾸조 | 혈액순환개선제 |
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Publication number | Publication date |
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KR0182395B1 (ko) | 1999-04-01 |
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