KR970006318A - 혈소판 응집 억제 펩타이드 및 그의 제조방법 - Google Patents

혈소판 응집 억제 펩타이드 및 그의 제조방법 Download PDF

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KR970006318A
KR970006318A KR1019950019685A KR19950019685A KR970006318A KR 970006318 A KR970006318 A KR 970006318A KR 1019950019685 A KR1019950019685 A KR 1019950019685A KR 19950019685 A KR19950019685 A KR 19950019685A KR 970006318 A KR970006318 A KR 970006318A
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South Korea
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platelet aggregation
aggregation inhibitory
inhibitory peptide
toxins
korean
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KR1019950019685A
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KR0142606B1 (ko
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강인철
정광희
이수정
김두식
김학대
윤영대
윤정혁
문홍모
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허영섭
재단법인 목암생명공학연구소
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Priority to KR1019950019685A priority Critical patent/KR0142606B1/ko
Priority to FR9510049A priority patent/FR2736266A1/fr
Priority to JP7230942A priority patent/JP2679780B2/ja
Publication of KR970006318A publication Critical patent/KR970006318A/ko
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Publication of KR0142606B1 publication Critical patent/KR0142606B1/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 한국산 살모사(Agkistrodon halys brevicaudus)의 독소로부터 분리한 혈소판 응집 억제 펩타이드 및 그의 제조방법에 관한 것이다. 한국산 살모사의 독소를 세린계 단백질 분해효소 친화성 컬럼에 주입하고 컬럼 투과액을 수득하여, 그 투과액을 음이온 교환수지, 젤 여과 및 HPLC 역상 크로마토그래피를 포함하는 정제과정을 통하여, 분자량 약 7,500Da의 혈소판 응집 억제 펩타이드를 정제하였다. 본 발명의 혈소판 응집 억제 펩타이드와 공지의 혈소판 응집 억제 펩타이드와의 혈소판 응집 억제활성을 비교한 결과, 본 발명의 혈소판 응집 억제 펩타이드가 가장 효과적으로 혈소판 응집을 억제함을 알 수 있었다. 따라서, 본 발명의 혈소판 응집 억제 펩타이드는 혈전중 치료제로 사용될 수 있을 것이다.

Description

혈소판 응집 억제 펩타이드 및 그의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (2)

  1. 한국산 살모사(Agkistrodon halys brevicaudus)의 독소로부터 분리한 하기와 같은 아미노산 서열을 갖는 혈소판 응집 억제 펩타이드 및 그의 기능적 등가물:
    EAGEE CDCGS PGNPC CDAAT CKLRQ GAQCA EGLCC DQCRF 40
    MKEGT ICRRA RGDDL DDYCN GISAG CPRNP FHA 73
  2. (ⅰ) 한국산 살모사(Agkistrodon halys brevicaudus)의 독소로부터 채취한 독소를 세린계 단백질 분해효소 친화성 컬럼에 주입하고 컬럼 투과액을 수득하는 공정; (ⅱ) 전기에서 수득한 투과액을 음이온 교환수지 크로마토그래피하는 공정; (ⅲ) 전기에서 수득한 활성분획을 젤 여과 크로마토그래피하는 공정; 및, (ⅳ) 전기에서 수득한 활성분획을 HPLC 역상 크로마토그래피하는 공정을 포함하는 한국산 살모사(Agkistrodon halys brevicaudus)의 독소로부터 제1항의 혈소판 응집 억제 펩타이드를 제조하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950019685A 1995-07-05 1995-07-05 혈소판 응집 억제 펩타이드 및 그의 제조방법 KR0142606B1 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
KR1019950019685A KR0142606B1 (ko) 1995-07-05 1995-07-05 혈소판 응집 억제 펩타이드 및 그의 제조방법
FR9510049A FR2736266A1 (fr) 1995-07-05 1995-08-24 Inhibiteur de l'agregation plaquettaire et procede pour preparer celui-ci
JP7230942A JP2679780B2 (ja) 1995-07-05 1995-09-08 血小板凝集抑制剤およびその製造方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950019685A KR0142606B1 (ko) 1995-07-05 1995-07-05 혈소판 응집 억제 펩타이드 및 그의 제조방법

Publications (2)

Publication Number Publication Date
KR970006318A true KR970006318A (ko) 1997-02-19
KR0142606B1 KR0142606B1 (ko) 1998-07-01

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KR1019950019685A KR0142606B1 (ko) 1995-07-05 1995-07-05 혈소판 응집 억제 펩타이드 및 그의 제조방법

Country Status (3)

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JP (1) JP2679780B2 (ko)
KR (1) KR0142606B1 (ko)
FR (1) FR2736266A1 (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002014488A1 (ko) * 2000-07-26 2002-02-21 Chung Kwang Hoe Novel protein derived from agkistrodon saxatilis emelianov and process for preparing the same

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6710030B1 (en) * 1993-10-22 2004-03-23 University Of Southern California Contortrostain (CN) and methods for its use in preventing metastasis and other conditions
US7220724B2 (en) 1993-10-22 2007-05-22 University Of Southern California Contortrostatin CN and methods for its use in preventing metastasis and other conditions
KR100335348B1 (ko) * 1998-06-23 2002-05-06 김두식 종양 혈관신생 억제기능을 가지는 살모신을 유효성분으로 함유하는 항암제
ATE455850T1 (de) * 1998-09-29 2010-02-15 Univ Southern California Contortrostatin(cn) sowie verfahren zu seiner verwendung in der prävention von metastasen und anderer zustände
AU2077001A (en) * 1999-12-10 2001-06-18 University Of Southern California Contortrostatin (cn) and methods for its use in preventing metastasis and other conditions
KR20020010188A (ko) * 2000-07-27 2002-02-04 정광회 뱀의 독소로부터 유래된 혈소판 응집억제 단백질 및 그의제조방법

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5182260A (en) * 1989-01-27 1993-01-26 Biogen, Inc. Dna sequences encoding snake venom inhibitors of platelet activation processes for producing those inhibitors and compositions using them
EP0382451A3 (en) * 1989-02-07 1991-05-29 Merck & Co. Inc. Viper venom polypeptides and variants
AU5939890A (en) * 1989-06-07 1991-01-07 Genentech Inc. Platelet aggregation inhibitors and related molecules
JPH05255395A (ja) * 1990-10-26 1993-10-05 Takeda Chem Ind Ltd ポリペプチドおよびその製造法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002014488A1 (ko) * 2000-07-26 2002-02-21 Chung Kwang Hoe Novel protein derived from agkistrodon saxatilis emelianov and process for preparing the same
US7033788B2 (en) 2000-07-26 2006-04-25 Kwang-Hoe Chung Protein derived from Agkistrodon saxatilis emelianov and process preparing the same

Also Published As

Publication number Publication date
KR0142606B1 (ko) 1998-07-01
FR2736266A1 (fr) 1997-01-10
JPH0920797A (ja) 1997-01-21
JP2679780B2 (ja) 1997-11-19

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