KR960031478A - Method for Purifying Ubiquitin-Fused Human Calcitonin - Google Patents
Method for Purifying Ubiquitin-Fused Human Calcitonin Download PDFInfo
- Publication number
- KR960031478A KR960031478A KR1019950002645A KR19950002645A KR960031478A KR 960031478 A KR960031478 A KR 960031478A KR 1019950002645 A KR1019950002645 A KR 1019950002645A KR 19950002645 A KR19950002645 A KR 19950002645A KR 960031478 A KR960031478 A KR 960031478A
- Authority
- KR
- South Korea
- Prior art keywords
- human calcitonin
- ubiquitin
- purifying
- fused human
- fraction
- Prior art date
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
본 발명은 효모에서 봉입체 형태로 발현된 유비퀴틴 융합인간칼시토닌을 정제하는 방법에 관한 것으로서, 세포를 파쇄하고 가용성 단백질을 제거한 후 봉입체를 회수 및 세척하여 이를 다시 용해시켜 분자량 한계가 10만인 여과막을 이용하여 불순 단백질을 제거하고, 다시 농축한 후 투석 및 pH 분획, 젤 여과 크로마토그라피와 소수성 크로마토그라피를 수행하는 것을 포함하는 방법에의하면, 유비퀴틴 융합 인간칼시토닌을 고순도로 정제할 수 있다.The present invention relates to a method for purifying ubiquitin fused human calcitonin expressed in the form of inclusion bodies in yeast. Ubiquitin fused human calcitonin can be purified with high purity by the method comprising removing the impure protein, concentrating again and then carrying out dialysis and pH fraction, gel filtration chromatography and hydrophobic chromatography.
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.
Claims (1)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950002645A KR960031478A (en) | 1995-02-14 | 1995-02-14 | Method for Purifying Ubiquitin-Fused Human Calcitonin |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
KR1019950002645A KR960031478A (en) | 1995-02-14 | 1995-02-14 | Method for Purifying Ubiquitin-Fused Human Calcitonin |
Publications (1)
Publication Number | Publication Date |
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KR960031478A true KR960031478A (en) | 1996-09-17 |
Family
ID=66531221
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019950002645A KR960031478A (en) | 1995-02-14 | 1995-02-14 | Method for Purifying Ubiquitin-Fused Human Calcitonin |
Country Status (1)
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KR (1) | KR960031478A (en) |
Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3984876A (en) * | 1975-01-16 | 1976-10-12 | Sutton Evelyn L | Article of clothing |
US4336187A (en) * | 1979-11-14 | 1982-06-22 | Armour Pharmaceutical Company | Purification of calcitonin by partition chromatography |
US4422967A (en) * | 1980-09-22 | 1983-12-27 | Armour Pharmaceutical Company | Purification of calcitonin by partition chromatography |
EP0146842A1 (en) * | 1983-12-08 | 1985-07-03 | Mitsubishi Petrochemical Co., Ltd. | Novel calcitonin and collection thereof |
KR880006263A (en) * | 1985-12-04 | 1988-07-22 | 예안 클라메르, 한스 루돌프 하우스 | Method for preparing a novel calcitonin derivative |
KR100274185B1 (en) * | 1993-02-25 | 2000-12-15 | 성재갑 | Human calcitonin fused with ubiquitin |
-
1995
- 1995-02-14 KR KR1019950002645A patent/KR960031478A/en not_active Application Discontinuation
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3984876A (en) * | 1975-01-16 | 1976-10-12 | Sutton Evelyn L | Article of clothing |
US4336187A (en) * | 1979-11-14 | 1982-06-22 | Armour Pharmaceutical Company | Purification of calcitonin by partition chromatography |
US4422967A (en) * | 1980-09-22 | 1983-12-27 | Armour Pharmaceutical Company | Purification of calcitonin by partition chromatography |
EP0146842A1 (en) * | 1983-12-08 | 1985-07-03 | Mitsubishi Petrochemical Co., Ltd. | Novel calcitonin and collection thereof |
KR880006263A (en) * | 1985-12-04 | 1988-07-22 | 예안 클라메르, 한스 루돌프 하우스 | Method for preparing a novel calcitonin derivative |
KR100274185B1 (en) * | 1993-02-25 | 2000-12-15 | 성재갑 | Human calcitonin fused with ubiquitin |
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