KR960031478A - Method for Purifying Ubiquitin-Fused Human Calcitonin - Google Patents

Method for Purifying Ubiquitin-Fused Human Calcitonin Download PDF

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Publication number
KR960031478A
KR960031478A KR1019950002645A KR19950002645A KR960031478A KR 960031478 A KR960031478 A KR 960031478A KR 1019950002645 A KR1019950002645 A KR 1019950002645A KR 19950002645 A KR19950002645 A KR 19950002645A KR 960031478 A KR960031478 A KR 960031478A
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KR
South Korea
Prior art keywords
human calcitonin
ubiquitin
purifying
fused human
fraction
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Application number
KR1019950002645A
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Korean (ko)
Inventor
한승재
김규돈
Original Assignee
성재갑
주식회사 Lg 화학
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Priority to KR1019950002645A priority Critical patent/KR960031478A/en
Publication of KR960031478A publication Critical patent/KR960031478A/en

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  • Peptides Or Proteins (AREA)

Abstract

본 발명은 효모에서 봉입체 형태로 발현된 유비퀴틴 융합인간칼시토닌을 정제하는 방법에 관한 것으로서, 세포를 파쇄하고 가용성 단백질을 제거한 후 봉입체를 회수 및 세척하여 이를 다시 용해시켜 분자량 한계가 10만인 여과막을 이용하여 불순 단백질을 제거하고, 다시 농축한 후 투석 및 pH 분획, 젤 여과 크로마토그라피와 소수성 크로마토그라피를 수행하는 것을 포함하는 방법에의하면, 유비퀴틴 융합 인간칼시토닌을 고순도로 정제할 수 있다.The present invention relates to a method for purifying ubiquitin fused human calcitonin expressed in the form of inclusion bodies in yeast. Ubiquitin fused human calcitonin can be purified with high purity by the method comprising removing the impure protein, concentrating again and then carrying out dialysis and pH fraction, gel filtration chromatography and hydrophobic chromatography.

Description

유비퀴틴 융합 인간 칼시토닌의 정제방법Method for Purifying Ubiquitin-Fused Human Calcitonin

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (1)

유비퀴틴 융합 인간 칼시토닌이 봉입체 형태로 발현된 효모세포를 파쇄하여 봉입체를 회수하고; 요소 용액에 용해시켜 여과 및 투석하고; 단백질 용액에서 pH 분획하고; 젤 여과 크로마토그라피 및 소수성 크로마토그라피를 수행하는 것을 포함하는 유비퀴틴 융합 인간 칼시토닌의 정제 방법.Ubiquitin fusion human calcitonin was crushed in yeast cells expressed in inclusion body form to recover the inclusion body; Dissolved in urea solution, filtered and dialyzed; PH fraction in protein solution; A method for purifying ubiquitin fused human calcitonin comprising performing gel filtration chromatography and hydrophobic chromatography. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임※ Note: The disclosure is based on the initial application.
KR1019950002645A 1995-02-14 1995-02-14 Method for Purifying Ubiquitin-Fused Human Calcitonin KR960031478A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019950002645A KR960031478A (en) 1995-02-14 1995-02-14 Method for Purifying Ubiquitin-Fused Human Calcitonin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019950002645A KR960031478A (en) 1995-02-14 1995-02-14 Method for Purifying Ubiquitin-Fused Human Calcitonin

Publications (1)

Publication Number Publication Date
KR960031478A true KR960031478A (en) 1996-09-17

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KR1019950002645A KR960031478A (en) 1995-02-14 1995-02-14 Method for Purifying Ubiquitin-Fused Human Calcitonin

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3984876A (en) * 1975-01-16 1976-10-12 Sutton Evelyn L Article of clothing
US4336187A (en) * 1979-11-14 1982-06-22 Armour Pharmaceutical Company Purification of calcitonin by partition chromatography
US4422967A (en) * 1980-09-22 1983-12-27 Armour Pharmaceutical Company Purification of calcitonin by partition chromatography
EP0146842A1 (en) * 1983-12-08 1985-07-03 Mitsubishi Petrochemical Co., Ltd. Novel calcitonin and collection thereof
KR880006263A (en) * 1985-12-04 1988-07-22 예안 클라메르, 한스 루돌프 하우스 Method for preparing a novel calcitonin derivative
KR100274185B1 (en) * 1993-02-25 2000-12-15 성재갑 Human calcitonin fused with ubiquitin

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3984876A (en) * 1975-01-16 1976-10-12 Sutton Evelyn L Article of clothing
US4336187A (en) * 1979-11-14 1982-06-22 Armour Pharmaceutical Company Purification of calcitonin by partition chromatography
US4422967A (en) * 1980-09-22 1983-12-27 Armour Pharmaceutical Company Purification of calcitonin by partition chromatography
EP0146842A1 (en) * 1983-12-08 1985-07-03 Mitsubishi Petrochemical Co., Ltd. Novel calcitonin and collection thereof
KR880006263A (en) * 1985-12-04 1988-07-22 예안 클라메르, 한스 루돌프 하우스 Method for preparing a novel calcitonin derivative
KR100274185B1 (en) * 1993-02-25 2000-12-15 성재갑 Human calcitonin fused with ubiquitin

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