KR920000926A - Purification Method of Human Growth Hormone from Genetically Recombined Yeast - Google Patents

Purification Method of Human Growth Hormone from Genetically Recombined Yeast Download PDF

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KR920000926A
KR920000926A KR1019900009924A KR900009924A KR920000926A KR 920000926 A KR920000926 A KR 920000926A KR 1019900009924 A KR1019900009924 A KR 1019900009924A KR 900009924 A KR900009924 A KR 900009924A KR 920000926 A KR920000926 A KR 920000926A
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South Korea
Prior art keywords
growth hormone
human growth
solution
guanidine
tris buffer
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KR1019900009924A
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Korean (ko)
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KR920005973B1 (en
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조중명
임국진
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최근선
주식회사 럭 키
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음.No content.

Description

유전자 재조합한 효모에서 인간 성장 호르몬의 정제 방법Purification Method of Human Growth Hormone from Genetically Recombined Yeast

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

제1도는 인간 성장 호르몬의 분리 정제 개요를 도표로 나타낸 것이고.Figure 1 is a graphical representation of the isolation and purification of human growth hormone.

제2도는 정제 단계별 인간 성장호르몬의 순도를 확인한 소듐도데실 설페이트-폴리아크릴아마이드 젤 전기영동(SDS-PAGE) 사진을 나타낸 것이다.Figure 2 shows sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) confirming the purity of human growth hormone in each step of purification.

Claims (3)

세포파괴과정, 불용해 침전물 분리과정, 불용해 침전물로 부터 인간 성장 호르몬 용해과정 및 음이온교환수지 컬럼 크로마트그래피 과징으로 인간 성장 호르몬을 정제하는데 있어서, 인간 성장 호르몬이 발현된 효모를 유리구슬과 함께 진탕시켜 세포를 파쇄한 다음, 세포 파쇄액을 원심 분리하여 용해된 물질을 제거하고 불용해 침전물을 구아니딘 트리스 완충용액에 현탁시켜 인간 성장 호르몬을 용해시킨 후, 이 용액을 원심 분리하여 그 상층액을 pH 3 내지 5사이의 초산 나트륨 용액에서 다른 단백질을 포함한 불순물을 침전시킨 다음, 이 용액을 원심분리하여 상층액을 취하여 트리스 완층용액에 투석한 후 음이온 교환수지 크로마트그래피하여 인간 성장 호르몬을 정제함을 특징으로 하는 인간 성장 호르몬의 정제 방법.In the process of cell destruction, separation of insoluble precipitate, purification of human growth hormone from insoluble precipitate, and purification of human growth hormone by anion exchange resin column chromatography, yeast expressing human growth hormone is combined with glass beads. After shaking to disrupt the cells, the cell lysate was centrifuged to remove the dissolved material, insoluble precipitate was suspended in guanidine tris buffer to dissolve human growth hormone, and the solution was centrifuged to separate the supernatant. Precipitate impurities containing other proteins in sodium acetate solution between pH 3 and 5, centrifuge the solution, take supernatant, dialysate into tris complete solution and purify human growth hormone by anion exchange resin chromatography Method for purifying human growth hormone, characterized in that. 제1항에 있어서, 구아니딘 트리스 완충액은 6M 구아니딘과 0.1M 탄산 암모니아로 구성되며 pH 9.6임을 특징으로 하는 인간 성장 호르몬의 정제 방법.The method of claim 1, wherein the guanidine tris buffer consists of 6M guanidine and 0.1M ammonia carbonate and has a pH of 9.6. 제1항에 있어서, 트리스 완충용액은 pH 7.5임을 특징으로 하는 인간 성장 호르몬의 정제 방법.The method of claim 1, wherein the Tris buffer solution has a pH of 7.5. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900009924A 1990-06-30 1990-06-30 Recovery of human growth hormone in yeast KR920005973B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019900009924A KR920005973B1 (en) 1990-06-30 1990-06-30 Recovery of human growth hormone in yeast

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019900009924A KR920005973B1 (en) 1990-06-30 1990-06-30 Recovery of human growth hormone in yeast

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KR920000926A true KR920000926A (en) 1992-01-29
KR920005973B1 KR920005973B1 (en) 1992-07-25

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KR920005973B1 (en) 1992-07-25

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