KR960031474A - 한외여과에 의한 단백질 용액으로부터의 바이러스의 제거방법 - Google Patents

한외여과에 의한 단백질 용액으로부터의 바이러스의 제거방법 Download PDF

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KR960031474A
KR960031474A KR1019960002859A KR19960002859A KR960031474A KR 960031474 A KR960031474 A KR 960031474A KR 1019960002859 A KR1019960002859 A KR 1019960002859A KR 19960002859 A KR19960002859 A KR 19960002859A KR 960031474 A KR960031474 A KR 960031474A
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virus
ligand
filtration
binding
size
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KR1019960002859A
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KR100421763B1 (ko
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베른하르트 디터
그뢰너 알브레흐트
노바크 토마스
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슈타인, 부크
베링베르케 아크티엔게젤샤프트
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis

Abstract

본 발명은 한외여과에 의한 액상용액, 일반적으로 단백질용액으로부터의 바이러스의 제거에 관한 것이다. 이것은 제거시키고자 하는 바이러스에 결합하는 고분자량 리간드, 바람직하게는 특이한 항체와 배양에 의해 상기 바이러스의 크기를 증가시킴으로써, 한편으로는 분리효과를 향상시키고 또 다른 한편으로는 이용되는 여과기에 따라 선택될 수 있는 보다 큰 세공직경으로 또한 작은 바이러스가 단백질 용액에 존재하는 보다 큰 단백질을 분자로부터 분리하도록 할 수 있으며 적절한 경우에는 여과율을 증가시킨다.

Description

한외여과에 의한 단백질 용액으로부터의 바이러스의 제거방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (9)

  1. 제거시키고자 하는 바이러스를 리간드에 결합시킴으로써 이의 크기를 증가시켜 여과에 의해 보유되도록함을 특징으로 하여, 단백질 용액으로부터 바이러스를 제거하는 방법.
  2. 제1항에 있어서, 리간드가, 화학적으로 또는 유전공학에 의해 변형될 수 있는 바이러스와 결합할 수 있는 항체이거나 또는 이의 일부분인 방법.
  3. 제1항에 있어서, 리간드가, 바이러스와 결합할 수 있는 모노클로날 항체이거나 또는 이의 일부분인 방법.
  4. 제1항에 있어서, 바이러스와 결합하는 리간드 또는 이의 일부가 화학적으로 또는 유전공학에 의해 변형되는 방법.
  5. 제1항에 있어서, 바이러스와 결합하는 리간드, 항체, 모노클로날 항체 또는 이들 일부가 여과전에 첨가되는 방법.
  6. 제1항에 있어서, 배양 단계가, 바이러스와 리간드가 결합할 수 있도록 여과전에 수행되는 방법.
  7. 제1항에 있어서, 한정된 세공크기를 갖는 동공의 섬유여과기 또는 막 여과기가 사용되는 방법.
  8. 제1항에 있어서, 여과를 근접 유동법으로 수행하는 방법.
  9. 제거시키고자 하는 물질을 리간드에 결합시킴으로써 이의 크기를 증가시켜 여과에 의해 보유되도록 함을 특징으로 하여, 원하지 않은 물질을 액상물질 혼합물로부터 제거하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임
KR1019960002859A 1995-02-09 1996-02-07 한외여과에 의한 단백질용액으로부터의 바이러스의 제거방법 KR100421763B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19504211A DE19504211A1 (de) 1995-02-09 1995-02-09 Entfernen von Viren durch Ultrafiltration aus Proteinlösungen
DE19504211.5 1995-02-09

Publications (2)

Publication Number Publication Date
KR960031474A true KR960031474A (ko) 1996-09-17
KR100421763B1 KR100421763B1 (ko) 2004-05-10

Family

ID=7753509

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Application Number Title Priority Date Filing Date
KR1019960002859A KR100421763B1 (ko) 1995-02-09 1996-02-07 한외여과에 의한 단백질용액으로부터의 바이러스의 제거방법

Country Status (9)

Country Link
US (2) US6391657B1 (ko)
EP (1) EP0727226B1 (ko)
JP (1) JPH08242849A (ko)
KR (1) KR100421763B1 (ko)
AT (1) ATE236659T1 (ko)
AU (1) AU708757B2 (ko)
CA (1) CA2169122A1 (ko)
DE (2) DE19504211A1 (ko)
ES (1) ES2193210T3 (ko)

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DE19709186C2 (de) 1997-03-06 1999-10-14 Medigene Ag Filtrationsverfahren zur Trennung von Viren
ES2277848T3 (es) * 1999-08-20 2007-08-01 Asahi Kasei Medical Co., Ltd. Membranas de filtracion para sustancias fisiologicamente activas.
AU1811301A (en) * 1999-12-02 2001-06-12 General Hospital Corporation, The Methods for removal, purification, and concentration of viruses, and methods of therapy based thereupon
CA2466627A1 (en) * 2001-11-28 2003-06-05 Sandoz Gmbh Chromatographic purification of recombinant human erythropoietin
US20040259076A1 (en) * 2003-06-23 2004-12-23 Accella Scientific, Inc. Nano and micro-technology virus detection method and device
US20090203008A1 (en) * 2006-06-08 2009-08-13 Ludmerer Steven W Rapid method to determine inhibitor sensitivity of NS3/4A protease sequences cloned from clinical samples
EP2550362B1 (en) 2010-03-25 2017-01-04 Oregon Health&Science University Cmv glycoproteins and recombinant vectors
CA2832109C (en) 2011-06-10 2021-07-06 Oregon Health & Science University Cmv glycoproteins and recombinant vectors
AU2012216792A1 (en) 2011-09-12 2013-03-28 International Aids Vaccine Initiative Immunoselection of recombinant vesicular stomatitis virus expressing HIV-1 proteins by broadly neutralizing antibodies
US9402894B2 (en) 2011-10-27 2016-08-02 International Aids Vaccine Initiative Viral particles derived from an enveloped virus
ES2631608T3 (es) 2012-06-27 2017-09-01 International Aids Vaccine Initiative Variante de la glicoproteína Env del VIH-1
US20150065381A1 (en) 2013-09-05 2015-03-05 International Aids Vaccine Initiative Methods of identifying novel hiv-1 immunogens
US10058604B2 (en) 2013-10-07 2018-08-28 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
US10174292B2 (en) 2015-03-20 2019-01-08 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers
US9931394B2 (en) 2015-03-23 2018-04-03 International Aids Vaccine Initiative Soluble HIV-1 envelope glycoprotein trimers

Family Cites Families (10)

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Publication number Priority date Publication date Assignee Title
US3896218A (en) * 1972-07-13 1975-07-22 Research Corp Radiommunoassay determining the hepatitis associated antigen content of blood
CA1221307A (en) * 1982-12-02 1987-05-05 Nobutaka Tani Adsorbent and process for preparing the same
US4692411A (en) * 1983-09-06 1987-09-08 Ghose Rabindra N Separation of specific biological cells by a biochemical filter
US5061237A (en) * 1985-07-02 1991-10-29 Cytomed Medizintechnik Gmbh Method of purifying whole blood
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DE4003543A1 (de) * 1990-02-06 1991-08-08 Orpegen Med Molekularbioforsch Verfahren zur abreicherung von viren in loesungen und zur bestimmung der abreicherungsrate von viren
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Also Published As

Publication number Publication date
EP0727226A2 (de) 1996-08-21
KR100421763B1 (ko) 2004-05-10
CA2169122A1 (en) 1996-08-10
EP0727226A3 (de) 1999-07-21
AU4440196A (en) 1996-08-15
EP0727226B1 (de) 2003-04-09
DE59610319D1 (de) 2003-05-15
US6391657B1 (en) 2002-05-21
DE19504211A1 (de) 1996-08-14
ATE236659T1 (de) 2003-04-15
ES2193210T3 (es) 2003-11-01
AU708757B2 (en) 1999-08-12
US20020068368A1 (en) 2002-06-06
JPH08242849A (ja) 1996-09-24

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