KR910005885A - 단백질 c의 활성화방법 - Google Patents

단백질 c의 활성화방법 Download PDF

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Publication number
KR910005885A
KR910005885A KR1019900013960A KR900013960A KR910005885A KR 910005885 A KR910005885 A KR 910005885A KR 1019900013960 A KR1019900013960 A KR 1019900013960A KR 900013960 A KR900013960 A KR 900013960A KR 910005885 A KR910005885 A KR 910005885A
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KR
South Korea
Prior art keywords
thrombin
protein
immobilized
human
bovine
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KR1019900013960A
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English (en)
Inventor
베티 얀 샤우-치
Original Assignee
레로이 위태커
일라이 릴리 앤드 캄파니
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Application filed by 레로이 위태커, 일라이 릴리 앤드 캄파니 filed Critical 레로이 위태커
Publication of KR910005885A publication Critical patent/KR910005885A/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6464—Protein C (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Immunology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Public Health (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

내용 없음

Description

단백질 C의 활성화방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (10)

  1. a) 불활성 단백질 C를 고정된 트롬빈과 접촉시키고, b)활성화된 단백질 C를 고정된 트롬빈 및 다른 오염물질로 부터 정제시키는 단계를 포함함을 특징으로 하는 단백질 C의 활성화 방법.
  2. 제1항에 있어서, 트롬빈이 소 트롬빈, 말트롬빈, 사람 트롬빈, 마우스 트롬빈 및 랫트 트롬빈으로 이루어진 그룹중에서 선택되는 방법.
  3. 제1항에 있어서, 트롬빈이 α-트롬빈, β-트롬빈 및-트롬빈으로 이루어진 그룹중에서 선택되는 방법.
  4. 제1항에 있어서, 트롬빈이 아가로즈상에 고정된 방법.
  5. 제1항에 있어서, 트롬빈이 세파로즈상에 고정된 방법.
  6. 제1항에 있어서, 단백질 C가 소 단백질 C, 사람단백질 C 및 사람단백질 C 유도체로 이루어진 그룹 중에서 선택되는 방법.
  7. 제6항에 있어서, 단백질 C가 사람단백질 C인 방법.
  8. 제2항에 있어서, 트롬빈이 소의 α-트롬빈인 방법.
  9. 제8항에 있어서, 소외 α-트롬빈이 아가로즈상에 고정된 방법.
  10. 제9항에 있어서, 단백질 C가 사람단백질 C인 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900013960A 1989-09-05 1990-09-05 단백질 c의 활성화방법 KR910005885A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US40351689A 1989-09-05 1989-09-05
US07403516 1989-09-05

Publications (1)

Publication Number Publication Date
KR910005885A true KR910005885A (ko) 1991-04-27

Family

ID=23596069

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019900013960A KR910005885A (ko) 1989-09-05 1990-09-05 단백질 c의 활성화방법

Country Status (12)

Country Link
EP (1) EP0416890A1 (ko)
JP (1) JPH0393799A (ko)
KR (1) KR910005885A (ko)
CN (1) CN1050043A (ko)
AU (1) AU6215290A (ko)
CA (1) CA2024598A1 (ko)
FI (1) FI904357A0 (ko)
HU (1) HU905784D0 (ko)
IE (1) IE903212A1 (ko)
IL (1) IL95585A0 (ko)
PT (1) PT95193A (ko)
ZA (1) ZA907039B (ko)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT397615B (de) * 1991-05-14 1994-05-25 Immuno Ag Arzneimittel enthaltend protein c
AT395596B (de) * 1991-06-20 1993-01-25 Immuno Ag Verfahren zur herstellung von aktiviertem protein c
DE4320294A1 (de) * 1993-06-18 1994-12-22 Immuno Ag Verwendung von humanem Protein C zur Verhinderung und Behandlung von Thrombozytenablagerungen
AT404597B (de) 1995-11-24 1998-12-28 Immuno Ag Verfahren zur herstellung von proteinen
AT404838B (de) * 1995-11-24 1999-03-25 Immuno Ag Herstellung von proteinen aus pro-proteinen durch fusionsproteine abgeleitet von furin oder furinanalogen
US5844087A (en) * 1996-11-05 1998-12-01 Bayer Corporation Method and device for delivering fibrin glue
DE19724239A1 (de) * 1997-06-09 1998-12-10 Max Planck Gesellschaft Dysprothrombinämie
AT407750B (de) * 1999-02-19 2001-05-25 Immuno Ag Verfahren zur herstellung einer vwf-präparation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0380508A4 (en) * 1987-05-18 1991-04-03 Integrated Genetics, Inc. Improved protein c molecules and method for making and activating same
US4908314A (en) * 1987-07-02 1990-03-13 American National Red Cross Protein C activator

Also Published As

Publication number Publication date
CN1050043A (zh) 1991-03-20
EP0416890A1 (en) 1991-03-13
AU6215290A (en) 1991-03-14
JPH0393799A (ja) 1991-04-18
ZA907039B (en) 1992-05-27
CA2024598A1 (en) 1991-03-06
HU905784D0 (en) 1991-03-28
IE903212A1 (en) 1991-03-13
FI904357A0 (fi) 1990-09-04
IL95585A0 (en) 1991-06-30
PT95193A (pt) 1991-05-22

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