KR960024367A - N-펩티드의 샌드위치 면역학적 측정법 - Google Patents

N-펩티드의 샌드위치 면역학적 측정법 Download PDF

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KR960024367A
KR960024367A KR1019950048118A KR19950048118A KR960024367A KR 960024367 A KR960024367 A KR 960024367A KR 1019950048118 A KR1019950048118 A KR 1019950048118A KR 19950048118 A KR19950048118 A KR 19950048118A KR 960024367 A KR960024367 A KR 960024367A
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monoclonal antibody
peptide
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antibody
amino acid
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요시토 누마타
히데히사 아사다
케이지 도히
타카히로 푸쿠나가
야수시 타니구치
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시오노 요시히코
시오노지 엔 씨오., 엘티디
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
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    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/26—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against hormones ; against hormone releasing or inhibiting factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/575—Hormones
    • G01N2333/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Brain natriuretic peptide [BNP, proBNP]; Cardionatrin; Cardiodilatin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2474/00—Immunochemical assays or immunoassays characterised by detection mode or means of detection
    • G01N2474/20—Immunohistochemistry assay
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y10S436/808—Automated or kit
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/811—Test for named disease, body condition or organ function
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/808—Materials and products related to genetic engineering or hybrid or fused cell technology, e.g. hybridoma, monoclonal products
    • Y10S530/809—Fused cells, e.g. hybridoma
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/827—Proteins from mammals or birds
    • Y10S530/829—Blood

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Abstract

본 발명은 N-펩티드의 상이한 부분을 인식하는 두 종류의 모노클로날 항체를 이용하여 N-펩티드를 신속하고 간편하게 측정하는 샌드위치 면역학적 측정법에 관한 것이다.
N-펩티드 또는 그것의 전구체를 측정하는 방법은 시료 및 N-펩티드의 일부를 인식하는 제1모노클로날항체를 함유하는 혼합물을 인큐베이션하고; 혼합물에 N-펩티드의 일부를 인식하는 표지화된 제2모노클로날 항체를 가한 다음, 추가로 인큐베이션하고, 그리고 얻어지는 항원-항체 복합체를 혼합물에서 검출하는 단계를 포함한다. 다르게는, 본 발명은 시료와 N-펩티드의 일부를 인식하는 제1모노클로날 항체 및 N-펩티드의 또 다른 부분을 인식하는 표지화된 제2모노클로날항체를 함유하는 혼합물을 인큐베이션하고; 그리고 얻어지는 항원-항체복합체를 혼합물에서 검출하는 단계를 포함한다.

Description

N-펩티드의 샌드위치 면역학적 측정법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (16)

  1. 시료 및 N-펩티드를 인식하는 제1모노클로날항체를 함유하는 혼합물을 인큐베이션하고; 혼합물에 N-펩티드를 인식하는 표지화된 제2모노클로날 항체를 가한다음, 추가로 인큐베이션하고; 그리고 얻어지는 항원-항체 복합체를 혼합물에서 검출하는 단계를 포함하는, N-펩티드 또는 그것의 전구체를 측정하는 방법.
  2. 시료와 N-펩티드를 인식하는 제1모노클로날 항체 및 N-펩티드를 인식하는 표지화된 제2모노클로날항체를 함유하는 혼합물을 인큐베이션하고; 그리고 얻어지는 항원-항체복합체를 혼합물에서 검출하는 단계를 포함하는, N-펩티드 또는 그것의 전구체를 측정하는 방법.
  3. 제1항 또는 2항에 있어서, 제1 또는 제2모노클로날항체가 N-펩티드의 43 위치에서부터 66위치까지의 아미노산 서열부분을 인식하는 것을 특징으로 하는 방법.
  4. 제3항에 있어서, 제1 또는 제2모노클로날항체가 7B6인 것을 특징으로 하는 방법.
  5. 제4항에 있어서, 제1 및 제2모노클로날 항체중의 하나는 7B6이고, 다른 하나는 N-펩티드의 아미노산 서열의 일부를 인식하며 인식부위가 7B6의 그것과는 다른 모노클로날 항체인 것을 특징으로 하는 방법.
  6. 제3항에 있어서, 제1 및 제2모노클로날항체중의 하나는 N-펩티드의 43위치에서부터 66위치까지의 아미노산부분을 인식하고, 다른 하나는 N-펩티드의 1위치에서부터 25위치까지의 아미노산 서열부분을 인식하는 것을 특징으로 하는 방법.
  7. 제6항에 있어서, 다른 하나의 모노클로날 항체가 KY-ANP-Ⅲ인 것을 특징으로 하는 방법.
  8. 제6항에 있어서, 제1모노클로날항체는 N-펩티드의 1위치에서부터 25위치까지의 아미노산 서열부분을 인식하고, 제2모노클로날항체는 N-펩티드의 43위치에서부터 66위치까지의 아미노산 서열부분을 인식하는 것을 특징으로 하는 방법.
  9. 제8항에 있어서, 제1모노클로날항체는 KY-ANP-Ⅲ이고, 제2모노클로날항체는 7B6인 것을 특징으로 하는 방법.
  10. 제1 또는 제2항에 있어서, 제2모노클로날항체가 방사성 동위원소, 효소, 금콜로이드, 형광물질, 또는 발광물질로 표지화된 것을 특징으로 하는 방법.
  11. 제10항에 있어서, 제2모노클로날 항체가 방사성 동위원소 또는 효소로 표지화된 것을 특징으로 하는 방법.
  12. N-펩티드의 43위치에서부터 66위치까지의 아미노산서열부분을 인식하는 모노클로날항체.
  13. 제12항에 있어서, 모노클로날 항체가 7B6인 것을 특징으로 하는 모노클로날 항체.
  14. 제12항 또는 13항의 모노클로날 항체를 생산하는 하이브리도마.
  15. 제14항에 있어서, 하이브리도마가 마우스 하이브리도마 7B6(FERM BP-4878)인 것을 특징으로 하는 하이브리도마.
  16. 제12항에 또는 13항의 모노클로날항체를 포함하는 N-펩티드 또는 그것의 전구체의 면역학적 측정용 키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019950048118A 1994-12-09 1995-12-09 N-펩티드의샌드위치면역학적측정법 KR100375564B1 (ko)

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KR100375564B1 KR100375564B1 (ko) 2003-05-12

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US (3) US5702910A (ko)
EP (1) EP0721105B1 (ko)
KR (1) KR100375564B1 (ko)
AT (1) ATE180058T1 (ko)
DE (1) DE69509626T2 (ko)
ES (1) ES2131279T3 (ko)
FI (1) FI119990B (ko)
NO (1) NO319913B1 (ko)

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US7648962B2 (en) * 2002-11-26 2010-01-19 Biocon Limited Natriuretic compounds, conjugates, and uses thereof
SG159387A1 (en) * 2002-11-26 2010-03-30 Biocon Ltd In Modified natriuretic compounds, conjugates, and uses thereof
DE10316583A1 (de) * 2003-04-10 2004-10-28 B.R.A.H.M.S Aktiengesellschaft Bestimmung eines midregionalen Proadrenomedullin-Teilpeptids in biologischen Flüssigkeiten zu diagnostischen Zwecken, sowie Immunoassays für die Durchführung einer solchen Bestimmung
WO2006076471A2 (en) * 2005-01-12 2006-07-20 Nobex Corporation Bnp conjugates and methods of use
DE102007010834A1 (de) 2007-03-03 2008-09-04 Brahms Aktiengesellschaft Diagnose und Risikostratifizierung von Herzinsuffizienz für NYHA I Patienten
DE102007022367A1 (de) 2007-05-07 2008-11-20 B.R.A.H.M.S Ag Verfahren zur Bestimmung von aminoterminalen pro-ANP bei übergewichtigen Patienten
EP2180322A1 (en) * 2008-10-22 2010-04-28 BRAHMS Aktiengesellschaft Prognostic biomarkers for the progression of primary chronic kidney disease
JP2014210761A (ja) * 2013-04-05 2014-11-13 株式会社シバヤギ 抗イヌｎ末端プロ心房性ナトリウム利尿ペプチド抗体ならびにそれを用いた免疫学的測定方法、および免疫学的測定用キット

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US4618600A (en) * 1984-04-19 1986-10-21 Biotechnology Research Associates, J.V. Novel polypeptide diuretic/vasodilators
JPH01216997A (ja) * 1988-02-24 1989-08-30 Fujisawa Pharmaceut Co Ltd 新規セフェム化合物ならびにその製造法
JP2724315B2 (ja) * 1988-02-29 1998-03-09 塩野義製薬株式会社 α−ＡＮＰを認識するモノクローナル抗体およびα−ＡＮＰの免疫学的測定法
GB8815796D0 (en) * 1988-07-02 1988-08-10 Bendix Ltd Gas compressing system
JP2561513B2 (ja) * 1988-07-04 1996-12-11 塩野義製薬株式会社 γ−ＡＮＰを認識するモノクローナル抗体

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DE69509626T2 (de) 2000-01-13
ATE180058T1 (de) 1999-05-15
US5859203A (en) 1999-01-12
NO954979L (no) 1996-06-10
EP0721105B1 (en) 1999-05-12
US5827674A (en) 1998-10-27
DE69509626D1 (de) 1999-06-17
ES2131279T3 (es) 1999-07-16
US5702910A (en) 1997-12-30
KR100375564B1 (ko) 2003-05-12
FI955894A (fi) 1996-06-10
NO954979D0 (no) 1995-12-07
FI955894A0 (fi) 1995-12-08
EP0721105A1 (en) 1996-07-10
FI119990B (fi) 2009-05-29
NO319913B1 (no) 2005-10-03

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