KR960004015B1 - Cleaning agents for skin - Google Patents

Abstract

The cleaning agent for a skin is produced by cultivating a bacillus subtilis ON-1 with a zizania latifolia O. stapf at 50˜85deg. C, ultrasonic sterilizing the cultivated material, extracting the material with 70% ethanol, and mixing the extract containing protease, amylase and lipase with a betaine surfactant and a purified water. The surfactant is pref. dimethyl cocoyl alkyl betaine, dimethyl dodecyl betaine, cocoyl fatty acid amino propyl betaine, dimethyl amine acetic acid betaine or 2-alkyl-N-carboxy methyl-N-hydroxy methyl imidazolium betaine. The cleaning agent has a good skin protecting effect, and is used in the mfr. of domestic cleaner, bathing agents and cosmetics.

Description

Skin Cleanser

The present invention relates to a skin cleaner having almost no harm to the skin, and furthermore having a skin protective action and a skin treatment effect, comprising the skin protection and cleanser as an essential ingredient and having a skin protection action and a skin treatment effect. Detergents, baths and cosmetics can be prepared.

Household cleaners, such as shampoos, kitchen detergents, residential detergents, laundry detergents, etc., cause roughness of hands due to the harmfulness of the cleaning ingredients themselves and cause intestinal diseases caused by eczema, dermatitis and absorption through the skin. In order to alleviate this problem, countermeasures such as a combination of a mild agent and a plant extract have been taken. However, sufficient cleaning power cannot be obtained with mild detergents such as methyltaurine or amino acid.

In general, bathing agents are mainly made of inorganic salts or fragrances, but very few of the original hot springs and strong effects can be obtained. The reason is that the skin becomes a stratum corneum, and this interferes with the lack of absorption of the active ingredient through the skin and the ion effect is not sufficiently obtained. In addition, the bathing agent which has the effect of treating skin disorders, such as atopic dermatitis, does not exist conventionally. In recent years, the addition of protease has been formulated for the purpose of improving skin luster, but it is not possible to obtain sufficient effects.

In addition, cosmetics, whether it is a skin cosmetic containing a skin cleaner, a finishing cosmetic, or a hair cosmetic, are basically different substances from the skin, causing troubles such as texture, eczema and the like. In recent years, the use of a substance closer to the components of the skin, or anti-inflammatory agents, anti-inflammatory enzymes, etc. have been mitigated, but it is still insufficient.

As with the prior art, there is a problem that the skin does not cease due to the toxicity absorbed through the irritating skin of the cleaner component, and on the contrary, there is a problem that a mild detergent cannot obtain sufficient cleaning power. . Bathing agents interfere with the stratum corneum of the skin, so that the absorption and ionic effect of active ingredients through the skin cannot be sufficiently obtained. On the contrary, the stratum corneum of the skin is removed without causing skin irritation and skin disorders. There is a problem that is difficult. Regarding cosmetics, there is a problem that the skin does not cease due to emulsifiers, preservatives, etc., regardless of the vegetable and minerals, and on the contrary, it is difficult to promote the absorption of the active ingredient through the skin without causing the skin. When the common problems of the above cleaning agents, bathing agents, and cosmetics are selected, it becomes clear that the problem that it is difficult to clean the skin after removing the stratum corneum of the skin without causing skin irritation and skin disorders becomes common. However, there has been no conventional skin cleaning agent having less skin irritation, less skin disorders, and having an older skin protection effect and skin treatment effect.

The problem to be solved by the present invention is to develop a skin protection and cleansing agent that can clean the skin without causing skin irritation and skin disorders, and furthermore has a skin protection and therapeutic effect. In addition, as essential ingredients, it can be formulated into household cleaners, baths and cosmetics to reduce skin irritation and skin troubles, and furthermore, to provide excellent skin cleaners, bath cleaners and cosmetics with excellent skin protection and skin treatment effects. To develop cleaners.

As a result of earnest research to solve this problem, Bacillus subtilis ON-1 bacteria (Japanese Makoma: Zizania Latifolia O. Stapf) was used as a medium. A mixture of betaine-based surfactants that sterilize the bacteria from the product cultured from the product obtained from the culture of the microorganism technology deposited at the Institute of Microbiology and Technology, purifies the stratum corneum of the skin without causing skin irritation and skin disorders. It has been found that it has a skin protection effect and a skin treatment effect, and thus has developed a skin protection and cleaning agent of the present invention.

Bacillus Subtilis ON-1 bacteria used in the present invention are fungi that produce endospores (Becillus). The microorganism was cultured at a relatively high temperature of 50 to 85 ° C. using a string of rice plants (Japanese name Makomo: Zizania Latifolia O. Stapf) as a medium, followed by the ultrasonication of the culture product to destroy the cells of the bacterium. do. Alternatively, the microorganism may be sterilized by filtration by the MF membrane method having a diameter of about 0.1 micron. The sterilized culture product is added to a beta-based surfactant as a homemade extract, a fluid extract, or as a dried fine powder to provide the skin protection and cleansing agent of the present invention.

As long as it is a component which is harmless to skin, the skin cleaner of this invention does not have a problem even if it adds a small amount of 1 or more types of components, and produces it.

Betaine-based surfactants used in the present invention include dimethyl cocoyl alkyl betaine, dimethyl dodecyl betaine, palm oil fatty acid amino propyl betaine, dimethyl amino Dimethyl amino acetic acid betaine, 2-alkyl-N-carboxymethyl-N-hydroxy methyl imidazolium betaine, and the like are preferably used. .

The enzymes contained in the skin protection and cleansing agent of the present invention are mainly proteases, amylases, and lipases, and these are Bacillus subtilis ON-1 (J. When Bacillus Subtilis ON-1) bacteria were cultured, they were produced inside and outside the cells. However, the enzyme produced by Bacillus subtilis ON-1 bacteria is purified by gel-permeation chromatography, membrane separation, salting out, dialysis, solvent separation, etc. One may be newly added and added to the skin protection and cleansing agent of the present invention.

Skin protection and cleaning agent of the present invention has a synergistic effect of the complex enzyme, polysaccharide, polyflavonoids and the like contained therein to have a protective effect and a therapeutic effect of the skin with a cleaning effect.

Although the skin protection and cleansing agent of the present invention functions as a skin protection and cleansing agent even when used as it is, it is used in combination with various surfactants having relatively low toxicity and high cleansing ability, so that there is almost no skin irritation and skin damage, It has a skin protection effect and a skin treatment effect, and can also make household cleaners such as shampoo, body shampoo, kitchen detergent, residential detergent, laundry detergent with high cleaning ability.

In addition, the skin care and cleansing agent of the present invention is formulated with inorganic salts, inorganic acids, organic acids, other low-toxic surfactants, other animal and vegetable extracts, fats and oils, squalene, squalane, sugars, fragrances, essential oils, etc. Can be.

The bathing agent of the present invention has an excellent effect in terms of a warming effect, a smooth feel of the skin, a sweating effect, and the like, compared to a conventional inorganic bathing agent or a simple protease enzyme. Moreover, it has the effect of treating skin disorders such as atopic dermatitis.

In addition, the skin protection and cleaning agent of the present invention is generally used in combination with cosmetic ingredients to moisturize the skin, prevent the roughness of the skin, prevent and relieve inflammation, promote absorption through the skin, and have strong skin protection. I can make it.

Example 1

(Skin protection and cleaning agent)

Cut 10 kg of fresh leaves of Joule (Japanese name Makomo: Zizania Latifolia O. Stapf), and add 0.1 g of Bacillus Subtilis ON-1 bacteria to the cells at 40 ° C for 3 days. After incubation, the culture was sonicated and then extracted with 30% of 70% ethanol to obtain an extract, and 2 parts by weight of the extract and 30 parts by weight of dimethyl palm alkyl betaine were mixed, and purified water was added to make the total amount 100 parts by weight.

Example 2

(Skin protection and cleaning agent)

Enzyme obtained by Gel Permeation Chromatography from Bacillus Subtilis ON-1.

Protease titer 30,000 F.L.U (casein reforming method) 0.5 part by weight

Amylase titer 35,000U 0.5 parts by weight

0.05 part by weight of lipase titer 30,000 U (R-COOH mol / min = 1 U)

It was added to the mixture of 2 weight part of extracts of Example 1, and 30 weight part of dimethyl palm alkyl betaines, and purified water was added and it made 100 weight part whole quantity.

Comparative Example 1

Dimethyl palm alkyl betaine 30% by weight aqueous solution

Comparative Example 2

Coconut caribbean 30% by weight aqueous solution

Experimental Example 1

Examples 1 and 2 The cleaning power, protein-modifying action, skin roughness sensation, and skin smoothness of the skin cleaners described in Comparative Examples 1 and 2 were examined by experiment.

The cleaning power was investigated by the number of sheets of dishes washed by the artificial contamination dish method. The dirt plate was coated with a watch glass with a diameter of 6 cm of 80 g of tallow, 20 parts of soybean oil, and 0.2 part of Sudan III solution. As a measure of protein denaturation, it was carried out by the Mercury Orange Method (Chemical 25 (1), 24 (1976)). The texture of skin texture and texture smoothness were evaluated by 5 panelers using 10 washers once a day for 15 days.

Skin texture: ▣ --- Nobody felt it.

○ --- 1 or 2 people felt.

■ -3 or 4 people felt.

▲ --- 5 or 6 people felt.

× --- 7 or more felt.

Skin Smoothness: ▣ --- The power source felt smooth enough.

○ --- 8-9 people felt the texture smooth enough.

■ --6-7 people felt the texture smooth enough.

▲ --- 4 to 5 people felt the texture smooth enough.

× --- 0 to 3 people felt a sufficiently smooth texture.

The results are as shown in Table 1.

TABLE 1

From the results of Table 1, it was confirmed that the skin protection and cleaning agent of the present invention have a higher safety to the skin, a skin smoothness texture, and an excellent cleaning power, compared to mild cleaning agents such as palm caribbean soap.

Example 3

[Shampoo & Kitchen Detergent (Household Cleaner)]

30 parts by weight of lauryl ether sodium sulfate

5 parts by weight of lauric acid diethanol amide

0.1 part by weight of citric acid

80 weight part of things which mixed the above raw material, and made 100 weight part with purified water were mixed, and 20 weight part of skin protection and detergent of Example 1 were mixed.

Comparative Example 3

[Shampoo & Kitchen Detergent (Household Cleaner)]

30 parts by weight of lauryl ether sodium sulfate

5 parts by weight of lauric acid diethanol amide

Citric acid 0.1 weight

The above raw materials are mixed and purified water is used to make 100 parts by weight.

Example 4

[Household Detergent and Laundry Detergent]

22 parts by weight of polyoxyethylene lauryl ether (E0 8 mol)

Coconut fatty acid diethanol amide

8 parts by weight

80 weight part of things which mixed the above raw material and made 100 weight part of purified water, and mixed it with 20 weight part of skin protection and cleaning agent of Example 1.

[Comparative Example 4]

[Household Detergent and Laundry Detergent]

22 parts by weight of polyoxyethylene lauryl ether (E0 8 mol)

Coconut fatty acid diethanol amide

8 parts by weight

The above raw materials were mixed and 100 parts by weight of purified water was added.

Experimental Example 2

In Example 3, 4, the washing | cleaning power, protein denaturation effect, skin roughness feeling, and skin smoothness feeling of each household cleaning agent of 3 and 4 were investigated as an experiment.

The cleaning power was investigated as the number of sheets of dishes washed by the artificial dish. The dirt plate was coated with a watch plate having a diameter of 6 cm with 1 g of five balls of 80 parts of tallow and 0.2 part of Sudan III solution. The protein denaturation was measured by the Mercury Orang Method (chemicals 25 (1), 24 (1976). For skin roughness and smoothness, 10 panelists were washed once a day. It was evaluated for 5 stages using 15 days.

Skin texture: ▣ --- Nobody felt it.

○ --- 1 or 2 people felt.

■ -3 or 4 people felt.

▲ --- 5 or 6 people felt.

× --- 7 or more felt.

Skin Smoothness: ▣ --- The power source felt smooth enough.

○ --- 8-9 people felt the texture smooth enough.

■ --6-7 people felt the texture smooth enough.

▲ --- 4 to 5 people felt the texture smooth enough.

× --- 0 to 3 people felt a sufficiently smooth texture.

The results are as shown in Table 2.

TABLE 2

From the results of Table 2, by combining the skin protection and cleaning agent of Example 1 into a general household cleaning agent, the effect of apparently mild cleaning (skin protection action) that is likely to cause hand roughness was confirmed, there is a skin smoothness texture, cleaning power It was also confirmed to improve.

Example 5

[Bath]

20 parts by weight of anhydrous sodium sulfate

5 parts by weight of sodium bicarbonate

1 part by weight of potassium carbonate

The above raw materials were mixed, 100 parts by weight of purified water was added to 80 parts by weight, and 20 parts by weight of the skin protection and cleaning agent of Example 1 were mixed.

Example 6

[Bath]

Skin Cleanser of Example 1

[Comparative Example 5]

[Bath]

20 parts by weight of anhydrous sodium sulfate

5 parts by weight of sodium bicarbonate

1 part by weight of potassium carbonate

The above raw materials were mixed and 100 parts by weight of purified water was added.

Experimental Example 3

Ten patients with atopic dermatitis complaining of itching by dissolving 20 g of each bath agent shown in Examples 5, 6 and Comparative Example 5 in a bath at 41 ° C. and 200 each became a panel agent, and the time until the bath was lowered to 5 ° C. 2 minutes after bathing by a magnetic thermometer (thermography) and skin smoothness were examined as an experiment. In addition, five levels of skin itching and skin smoothness were evaluated, and an average of 10 panelists was described for the sweating effect of heat retention.

Skin texture: ▣ --- Itching all over.

○ --- 8 or 9 had itching.

■ --- 6-7 had itching.

▲ --- 4 or 5 people have itching.

× --- 0 ~ 3 people had itching.

Skin Smoothness: ▣ --- The power source felt smooth enough.

○ --- 8-9 people felt the texture smooth enough.

■ --6-7 people felt the texture smooth enough.

▲ --- 4 to 5 people felt the texture smooth enough.

× --- 0 to 3 people felt a sufficiently smooth texture.

The results are shown in Table 3.

TABLE 3

From the results in Table 3, when the skin protection and cleaning agent of Example 6 (= Example 1) were used as the bathing agent, it was effective in treating atopic dermatitis, and the skin smoothness and texture were also good.

By combining this skin protection and cleansing agent with a general bathing agent, it can be found that the skin treatment effect and the skin smoothing effect can be obtained compared to the one that is not formulated, and the unnecessary dead skin on the surface of the skin is removed. The effect was improved together.

Example 7

[Cosmetics (cosmetics)]

Glycerin (glycerin) 5 parts by weight

0.2 parts by weight of oleyl alchol

3 parts by weight of polyoxyethylene oleyl ether

15 parts by weight of ethyl alcohol

0.1 part by weight

0.05 parts by weight of propyl p-hydroxybenzoate

0.05 parts by weight of butyl p-hydroxybenzoate

0.02 parts by weight of sodium edetate

The above raw materials were mixed, and purified water was added and made into 100 weight part, 90 weight part and 10 weight part of skin protection and detergent of Example 1 were mix | blended.

Comparative Example 6

[Cosmetics (cosmetics)]

Glycerin (glycerin) 5 parts by weight

0.2 parts by weight of oleyl alchol

3 parts by weight of polyoxyethylene oleyl ether

15 parts by weight of ethyl alcohol

0.1 part by weight

0.05 parts by weight of propyl p-hydroxybenzoate

0.05 parts by weight of butyl p-hydroxybenzoate

0.02 parts by weight of sodium edetate

The above raw materials were mixed and 100 parts by weight of purified water was added.

Example 8

[Cream (Cosmetics)]

Beeswax 2 parts by weight

15 parts by weight of stearic acid

5 parts by weight of oleyl alchol

Squalane 5 parts by weight

Polyoxyethylene cetyl ether

4 parts by weight

15 parts by weight of glycerin

0.7 parts by weight of triethanol amine

0.3 parts by weight of perfume

0.5 parts by weight of butyl hydroxy anisol

1 part by weight of butyl p-hydroxybenzoate

The above raw materials were mixed, and purified water was added to make 100 parts by weight, and 90 parts by weight of the skin protection and cleaning agent of Example 1 was blended.

Comparative Example 7

[Cream (Cosmetics)

Beeswax 2 parts by weight

15 parts by weight of stearic acid

5 parts by weight of oleyl alchol

Squalane 5 parts by weight

Polyoxyethylene cetyl ether

4 parts by weight

15 parts by weight of glycerin

0.7 parts by weight of triethanol amine

0.3 parts by weight of perfume

0.5 parts by weight of butyl hydroxy anisol

1 part by weight of butyl p-hydroxybenzoate

The above raw materials were mixed and 100 parts by weight of purified water was added.

Experimental Example 4

Example 7,8 The cosmetics obtained in Comparative Examples 6 and 7 were used by 10 panelists once a day for 1 month, and the skin roughness feeling was evaluated in seven stages, and the skin moist feeling texture was evaluated in five stages.

Rough skin texture: ▣ ----- No power was felt.

○ ----- 1-2 people felt.

■ ----- 3-4 people felt.

▲ ----- 5-6 people felt.

× ----- 7 or more people felt.

×× --- Redness (redness of the skin) appeared in two or more people.

××× --Troubles such as eczema and itching appeared in two or more people.

Skin Moisturizing Feel: ▣ --- The power felt fresh enough.

○ --- 8-9 people felt fresh enough.

■ --6-7 people felt fresh enough.

▲ --- 4-5 people felt fresh enough.

× --- 0 to 3 people felt the texture sufficiently fresh.

The results are shown in Table 4.

TABLE 4

As shown in Table 4, by combining the skin protection and cleaning agent of Example 1 with general cosmetics, a moist texture can be obtained, the moisturizing effect is increased, and the effect of preventing skin roughness was also confirmed.

The skin protection and cleaning agent of the present invention can clean the skin without causing skin irritation and skin disorders, and also has a protective and therapeutic effect on the skin. In addition, when the skin protection and cleaning agent of the present invention is used as an essential ingredient in household cleaning agents, bathing agents, and cosmetics, skin cleaning agents, bathing agents, and cosmetics having excellent performance with less skin irritation and skin disorders, and also have skin protection and skin treatment effects I can make it.

Claims (1)

Bacillus Subtilis ON-1 (Bacillus Subtilis ON-1) is a common strain deposited in the microbial technology laboratory of the Japan Institute of Technology. ) A skin cleanser obtained by culturing bacteria at 50-85 ° C. and then mixing an extract containing a protease, amylase, and lipase with a betaine-based surfactant.