KR950700403A - 조혈 간세포의 배양 및 그것의 유전공학(culturing of hematopoietic stem cells and their genetic engineering) - Google Patents

조혈 간세포의 배양 및 그것의 유전공학(culturing of hematopoietic stem cells and their genetic engineering)

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KR950700403A
KR950700403A KR1019940703107A KR19940703107A KR950700403A KR 950700403 A KR950700403 A KR 950700403A KR 1019940703107 A KR1019940703107 A KR 1019940703107A KR 19940703107 A KR19940703107 A KR 19940703107A KR 950700403 A KR950700403 A KR 950700403A
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medium
hematopoietic stem
cells
human hematopoietic
stem cells
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KR1019940703107A
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쉬 츄-치이
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죤 제이. 쉬바르츠
시스테믹스, 인코포레이티드
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Publication of KR950700403A publication Critical patent/KR950700403A/ko

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/22—Colony stimulating factors (G-CSF, GM-CSF)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2303—Interleukin-3 (IL-3)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2306—Interleukin-6 (IL-6)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
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    • C12N2502/00—Coculture with; Conditioned medium produced by
    • C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
    • C12N2502/1394—Bone marrow stromal cells; whole marrow
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    • C12N2510/00—Genetically modified cells

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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

사람 조혈 간세포는 장기간 배양중에, 특히 적절한 간질 셀라인과의 공동 배양중에, 특히 백혈병 억제 인자의 존재하에, 조혈 간세포 자체로서 또는 다른 이자와 조합하여 성장할 수 있다. 세포는 생체내 T-세포검정 및 메틸셀룰로오스에서 다른 계통의 콜로니들을 형성하는 능력에 의해 증명되는바, 그것의 기능을 보유하는 것으로 밝혀진다. 세포의 형질전환은 바이러스로 이루어질 수 있으며, 이때 백혈병 억제인자와 임의로 다른 조혈인자의 존재하에 개선된 결과가 얻어진다.

Description

조혈 간세포의 배양 및 그것의 유전공학(CULTURING OF HEMATOPOIETIC STEM CELLS AND THEIR GENETIC ENGINEERING)
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (20)

  1. 사람 조혈 간세포를 최소한 약 10ng/ml 의 백혈병 억에 인자를 포함하는 배지와 최소한 12시간동안 접촉시키는 단계; 그리고 상기 간세포를 상기 간세포의 성장을 지지할 수 있는 배지에서 성장시키는 단계를 포함하는 것을 특징으로 하는, 사람 조혈 간세포를 배양배지에서 성장시키는 방법.
  2. 제 1 항에 있어서, 상기 백혈병 억제 인자가 약 2 내지 20ng/ml의 양으로 존재하는 것을 특징으로 하는 방법.
  3. 제 1 항에 있어서, 상기 접촉이 최소한 1ng/ml의 IL-3, IL-6, GM-CSF, 또는 강인자중 하나와 이루어지는 것을 특징으로 하는 방법.
  4. 제 1 항에 있어서, 상기 성장은 간질 셀라인으로부터 부터의 조건 배지의 존재하에 이루어지는 것을 특징으로 하는 방법.
  5. 제 4 항에 있어서, 상기 간질 셀라인이 상기 배지에 존재하는 것을 특징으로 하는 방법.
  6. 사람 조혈 간세포를, 약 2 내지 20ng/ml의 백혈병 억제 인자와 최소한 1ng/ml의 하나의 IL-3, IL-6, GM-CSF 또는 강인자를 포함하는 배지와 최소 12시간 동안 접촉시키는 단계와, 상기 간세포를 상기 간세포의 성장을 지지할 수 있는 배지에서 성장시키는 단계를 포함하는 것을 특징으로 하는 배양배지에서 사람 조혈 간세포를 성장시키는 방법.
  7. 제 6 항에 있어서, 상기 성장은 간질 셀라인으로 부터의 조건 배지의 존재하에 이루어지는 것을 특징으로 하는 방법.
  8. 제 7 항에 있어서, 상기 간질 셀라인이 상기 배지에 존재하는 것을 특징으로 하는 방법.
  9. 제 6 항에 있어서, 상기 백혈병 억제 인자가 상기 성장 지지배지중에서 유지되는 것을 특징으로 하는 방법.
  10. 실질적으로 전용 세포가 없는 사람 조혈 간세포를, 사람세포에 대해 굴성이고, 사람 조혈 세포에서 전사될 수 있는 관심의 DNA 서열을 포함하는 레트로바이러스 벡터와, 최소한 10ng/ml의 백혈병 억제 인자를 포함하는 배지중에서 상기 바이러스가 상기 간세포에 들어가기에 충분한 시간동안 접촉시키는 것으로 이루어지는, 사람 조혈 간세포를 형질전환하기 위한 방법.
  11. 제10항에 있어서, 상기 배지가 또한 최소한 1ng/ml의 양으로 최소한 하나의 IL-3, IL-6, GM-CSF 또는 강인자를 포함하는 것을 특징으로 하는 방법.
  12. 제10항에 있어서, 상기 DNA 서열이 사람 조혈세포에서 전살될 수 있는 유전자인 것을 특징으로 하는 방법.
  13. 제10항에 있어서, 추가로 배양중에 상기 DNA 서열을 포함하는 사람 조혈 간세포를 간세포의 성장을 지지할 수 있는 배지에서 성장시키는 단계를 포함하는 방법.
  14. 제13항에 있어서, 상기 성장 지지배지가 간질 셀라인으로 부터의 조건 배지인 것을 특징으로 하는 방법.
  15. 제14항에 있어서, 상기 간질 셀라인이 상기 배지에 존재하는 것을 특징으로 하는 방법.
  16. 제15항에 있어서, 상기 백혈병 억제 인자가 상기 성장 지지배지중에서 유지되는 것을 특징으로 하는 방법.
  17. DNA의 통합의 결과로 관심의 DNA 서열을 포함하는 실질적으로 전용 조혈 세포가 없는 사람 조혈 간세포 조성물.
  18. 제17항에 있어서, 상기 관심의 DNA 서열이 조혈 세포에서 전사될 수 있는 유전자인 것을 특징으로 하는 사람 조혈 간세포 조성물.
  19. 제18항에 있어서, 상기 유전자 G418내성 유전자인 것을 특징으로 하는 사람 조혈 간세포 조성물.
  20. 제17항의 사람 조혈 간세포로 부터 유래되는 사람 조혈 세포.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019940703107A 1992-03-04 1993-03-03 조혈 간세포의 배양 및 그것의 유전공학(culturing of hematopoietic stem cells and their genetic engineering) KR950700403A (ko)

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US84678292A 1992-03-04 1992-03-04
US07/846,782 1992-03-04
PCT/US1993/001852 WO1993018137A1 (en) 1992-03-04 1993-03-03 Culturing of hematopoietic stem cells and their genetic engineering

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EP (1) EP0631618A4 (ko)
JP (1) JPH07504331A (ko)
KR (1) KR950700403A (ko)
AU (2) AU680406B2 (ko)
CA (1) CA2131368A1 (ko)
WO (1) WO1993018137A1 (ko)

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US5605822A (en) * 1989-06-15 1997-02-25 The Regents Of The University Of Michigan Methods, compositions and devices for growing human hematopoietic cells
US5437994A (en) * 1989-06-15 1995-08-01 Regents Of The University Of Michigan Method for the ex vivo replication of stem cells, for the optimization of hematopoietic progenitor cell cultures, and for increasing the metabolism, GM-CSF secretion and/or IL-6 secretion of human stromal cells
US5635386A (en) * 1989-06-15 1997-06-03 The Regents Of The University Of Michigan Methods for regulating the specific lineages of cells produced in a human hematopoietic cell culture
US6242417B1 (en) 1994-03-08 2001-06-05 Somatogen, Inc. Stabilized compositions containing hemoglobin
US5631219A (en) * 1994-03-08 1997-05-20 Somatogen, Inc. Method of stimulating hematopoiesis with hemoglobin
US7083979B1 (en) 1994-03-25 2006-08-01 Indiana University Foundation Methods for enhanced retroviral-mediated gene transfer
AU3635695A (en) * 1994-09-19 1996-04-09 Board Of Trustees Of The Leland Stanford Junior University Methods for genetically modifying hematopoietic stem cells
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EP0821740A1 (en) * 1995-04-20 1998-02-04 Chiron Corporation High efficiency ex vivo transduction of hematopoietic stem cells by recombinant retroviral preparations
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GB9518606D0 (en) 1995-09-12 1995-11-15 Inst Of Psychiatry Neural transplantation
ES2265153T3 (es) * 1995-09-29 2007-02-01 Indiana University Research And Technology Corporation Metodos para una transferencia de adn mediada por virus potenciada usando moleculas con dominios de union a virus y celulas.
US5922597A (en) * 1995-11-14 1999-07-13 Regents Of The University Of Minnesota Ex vivo culture of stem cells
GB9807935D0 (en) 1998-04-14 1998-06-10 Univ Edinburgh Lineage specific cells and progenitor cells
WO1997029183A2 (en) * 1996-02-08 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Methods and compositions for transforming dendritic cells and activating t cells
US6734014B1 (en) 1996-02-08 2004-05-11 The United States Of America As Represented By The Department Of Health And Human Services Methods and compositions for transforming dendritic cells and activating T cells
US5811297A (en) * 1996-03-07 1998-09-22 Amba Biosciences, Llc Immortalized hematopoietic cell lines, cell system thereof with stromal cells, in vitro, ex vivo and in vivo uses, & in vitro generation of dendritic cells and macrophages
JP3962459B2 (ja) * 1997-10-28 2007-08-22 麒麟麦酒株式会社 造血幹細胞の分化・増殖調節方法
US20020034819A1 (en) 1998-02-23 2002-03-21 Alan K. Smith Human lineage committed cell composition with enhanced proliferative potential, biological effector function, or both; methods for obtaining same; and their uses
CA2338344A1 (en) * 1998-05-29 1999-12-02 Case Western Reserve University Hematopoietic progenitor cell gene transduction
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CA2369085A1 (en) * 1999-05-14 2000-11-23 City Of Hope Ex vivo expansion of mammalian hematopoietic stem cells
AU1753101A (en) * 1999-10-29 2001-05-14 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Method of in vitro T cell differentiation of CD34+ progenitor cells
DE602005002430T2 (de) 2004-09-30 2008-06-19 Reneuron Ltd., Guildford Zelllinie
US20160024473A1 (en) 2013-03-08 2016-01-28 Cells For Cells Menstrual stems cells for the efficient support and expansion of cd34+ cd133+ hematopoietic stem cells in vitro

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US5366888A (en) * 1990-07-09 1994-11-22 Amrad Corporation Limited Enhanced maintenance of pregnancy using leukaemia inhibitory factor in embryo culturing

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EP0631618A4 (en) 1995-09-27
CA2131368A1 (en) 1993-09-16
EP0631618A1 (en) 1995-01-04
AU3783993A (en) 1993-10-05
AU4369097A (en) 1998-02-12
WO1993018137A1 (en) 1993-09-16
JPH07504331A (ja) 1995-05-18

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