KR950010747A - 바이오캡슐화(bioencapsulation)를 이용한 미생물살충제의 제조방법 - Google Patents

바이오캡슐화(bioencapsulation)를 이용한 미생물살충제의 제조방법 Download PDF

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KR950010747A
KR950010747A KR1019930022037A KR930022037A KR950010747A KR 950010747 A KR950010747 A KR 950010747A KR 1019930022037 A KR1019930022037 A KR 1019930022037A KR 930022037 A KR930022037 A KR 930022037A KR 950010747 A KR950010747 A KR 950010747A
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gel matrix
natural
biological control
natural gel
protein
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KR1019930022037A
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KR970007083B1 (ko
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복성해
김성욱
권용국
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김은영
한국과학기술연구원
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Priority to KR1019930022037A priority Critical patent/KR970007083B1/ko
Priority to CA002118267A priority patent/CA2118267C/en
Publication of KR950010747A publication Critical patent/KR950010747A/ko
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Publication of KR970007083B1 publication Critical patent/KR970007083B1/ko

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22—Bacillus
    • A01N63/23—B. thuringiensis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Biomedical Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

본 발명은 단백질이 풍부한 콩류의 천연 고분자내에 생물학적 방제 인자(바실러스 터린지엔시스 세포, 포자, B.T 독소)를 바이오캡슬화함을 포함하는 미생물 농약의 간단하고 경제적인 제조 방법 및 상기 방법에 의해 제조된 생분해 가능하고 환경적으로 안전한 미생물살충제에 관한 것이다.

Description

바이오캡슐화(bioencapsulation)를 이용한 미생물살충제의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (7)

  1. 가) 단백질 함량이 50중량% 이상인 단백질이 풍부한 콩류의 천연 고분자를 물과 혼합하고 100℃ 이상으로 가열하여 분쇄하거나 부드럽게하여 균일한 겔 매트릭스를 만듬으로써 단백질이 풍부한 천연겔 매트릭스를 제조하고, 나) 상기 천연겔 매트릭스를 121℃에서 가압 멸균시키고, 다) 상기 멸균된 천연겔 매트릭스를 상온으로 냉각시키고, 라) 바실러스 터링지엔시스 세포, 포자 혹은 B.T. 독소 등이나 이들의 복합체를 포함하는 생물학적 방제 인자를 상기 냉각된 천연겔 매트릭스와 균일하게 혼합하고, 마) 수득된 천연겔 매트릭스-생물학적 방제 인자 복합체를 건조 또는 동결건조시킨 후 분쇄하여 분말, 입자 또는 펠릿을 제조하는 단계들을 포함하는 생물학적 방제 인자가 바이오캡슐화된 미생물살충제를 제조하는 방법.
  2. 제1항에 있어서, 상기 단백질이 풍부한 고분자가 콩류, 견과류, 땅콩류, 지방 종자, 이들의 분말 또는 부산물, 면실박 또는 그의 혼합물을 포함하는 방법.
  3. 제1항에 있어서, 가)의 천연겔중의 고형분 함량이 5∼40%인 방법.
  4. 제1항에 있어서, 가) 단계에서 상기 천연 고분자를 100℃∼121℃로 가열하는 방법.
  5. 제1항에 있어서, 라) 단계에서 생물학적 방제 인자를 천연겔 매트릭스 1g 당 104~1014세포 또는 포자의 농도로 가하는 방법.
  6. 제1항에 있어서, 마) 단계에서 천연겔-생물학적 방제 인자 복합체를 20~50℃로 건조시키는 방법.
  7. 제1항 내지 제6항중 어느 한 항의 방법에 의해 제조된 캡슐화된 미생물살충제.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019930022037A 1993-10-22 1993-10-22 바이오캡슐화(bioencapsulation)를 이용한 미생물살충제의 제조방법 KR970007083B1 (ko)

Priority Applications (2)

Application Number Priority Date Filing Date Title
KR1019930022037A KR970007083B1 (ko) 1993-10-22 1993-10-22 바이오캡슐화(bioencapsulation)를 이용한 미생물살충제의 제조방법
CA002118267A CA2118267C (en) 1993-10-22 1994-10-17 Bioencapsulated biopesticides and process for the manufacture thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930022037A KR970007083B1 (ko) 1993-10-22 1993-10-22 바이오캡슐화(bioencapsulation)를 이용한 미생물살충제의 제조방법

Publications (2)

Publication Number Publication Date
KR950010747A true KR950010747A (ko) 1995-05-15
KR970007083B1 KR970007083B1 (ko) 1997-05-02

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KR1019930022037A KR970007083B1 (ko) 1993-10-22 1993-10-22 바이오캡슐화(bioencapsulation)를 이용한 미생물살충제의 제조방법

Country Status (2)

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KR (1) KR970007083B1 (ko)
CA (1) CA2118267C (ko)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990041258A (ko) * 1997-11-21 1999-06-15 구광시 해도형 복합 중공 섬유
KR20010045115A (ko) * 1999-11-03 2001-06-05 구광시 열가소성 극세섬유
KR100470232B1 (ko) * 2002-06-04 2005-02-05 서형원 미생물 전달매체 및 그를 포함하는 미생물농약의 제조방법

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109497050B (zh) * 2018-11-28 2020-08-18 浙江大学 一种多粘类芽孢杆菌发酵物及其制备方法和应用

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR19990041258A (ko) * 1997-11-21 1999-06-15 구광시 해도형 복합 중공 섬유
KR20010045115A (ko) * 1999-11-03 2001-06-05 구광시 열가소성 극세섬유
KR100470232B1 (ko) * 2002-06-04 2005-02-05 서형원 미생물 전달매체 및 그를 포함하는 미생물농약의 제조방법

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Publication number Publication date
CA2118267A1 (en) 1995-04-23
CA2118267C (en) 1997-12-30
KR970007083B1 (ko) 1997-05-02

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