KR930008462A - 치주질환과 관련된 미생물의 검증방법에서 높은 pH에서의 추출로 단백질을 보호하는 용도 및 이에 유용한 키트 - Google Patents

치주질환과 관련된 미생물의 검증방법에서 높은 pH에서의 추출로 단백질을 보호하는 용도 및 이에 유용한 키트 Download PDF

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KR930008462A
KR930008462A KR1019920018381A KR920018381A KR930008462A KR 930008462 A KR930008462 A KR 930008462A KR 1019920018381 A KR1019920018381 A KR 1019920018381A KR 920018381 A KR920018381 A KR 920018381A KR 930008462 A KR930008462 A KR 930008462A
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periodontal disease
antigen
protective
extraction
microorganisms associated
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KR1019920018381A
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포터 보이어 브래들리
버나드 콘테스터블 폴
앤토니 스나이더 브라이언
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알프레드 피. 로렌조
이스트만 코닥 캄파니
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Publication of KR930008462A publication Critical patent/KR930008462A/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/56955—Bacteria involved in periodontal diseases
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/961—Chemistry: molecular biology and microbiology including a step of forming, releasing, or exposing the antigen or forming the hapten-immunogenic carrier complex or the antigen per se
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

고감도이며 매우 낮은 백그라운드 및 다수의 밀접하게 관련되 항원들 중에서 매우 낮은 교차-반응성을 나타내는, 치주질환과 관련된 미생물의 검증방법이 개발되었다. 항원은 비교적 높은 pH에서, 추출전 또는 추출직후에 추출하고, 항원-함유시료는 적어도 약 0.2중량%의 비-면역반응성 보호 단백질을 갖는 보호 조성물과 혼합한다. 생성 혼합물의 pH는 목적한는 항원에 특이적인 항체와 접촉시 높게 유지시킨다.

Description

치주질환과 관련된 미생물의 검증방법에서 높은 pH에서의 추출로 단백질을 보호하는 용도 및 이에 유용한 키트
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (2)

  1. A. 적어도 8의 pH로 완충시킨 추출 조성물을 사용하여 치주질환과 관련된 미생물로부터 항원을 추출하고, B. 상기 추출 조성물을, 적어도 0.2중량%의 비-면역반응성 보호 단백질을 갖는 혼합물을 제공하기에 충분한 양의 상기 단백질로 필수적으로 이루어진 보호 조성물과 혼합(이때 상기 단백질의 보호 성질은 상기 혼합물의 높은 pH 또는 상기 내에 존재하는 임의의 계면활성제에 의해서 불리한 영향을 받지 않는다)하고, C. B단계에서 제조된 혼합물의 pH를 8이하로 낮추지 않으면서, 상기 혼합물을 상기 항원에 특이적인 항체와 접촉시켜 면역학적 복합체를 제조하고, D. 상기 복합체를 검출하는 단계들을 포함하는, 치주질환과 관련된 미생물의 검증방법.
  2. 별도의 패키지로, (a) 적어도 8의 pH로 완충시킨 추출 조성물, (b) 치주질환과 관련된 미생물에 대해 특이적인 수용성 항체 및 (c) 필수적으로 비-면역반응성 보호 단백질로 이루어진 조성물을 포함하는 진단 시험 키트.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920018381A 1991-10-08 1992-10-07 치주질환과 관련된 미생물의 검증방법에서 높은 pH에서의 추출로 단백질을 보호하는 용도 및 이에 유용한 키트 KR930008462A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US773,064 1991-10-08
US07/773,064 US5399484A (en) 1991-10-08 1991-10-08 Use of blocking protein with high pH extraction in method to determine a microorganism associated with periodontal disease and kit useful therefor

Publications (1)

Publication Number Publication Date
KR930008462A true KR930008462A (ko) 1993-05-21

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KR1019920018381A KR930008462A (ko) 1991-10-08 1992-10-07 치주질환과 관련된 미생물의 검증방법에서 높은 pH에서의 추출로 단백질을 보호하는 용도 및 이에 유용한 키트

Country Status (7)

Country Link
US (1) US5399484A (ko)
EP (1) EP0537828A1 (ko)
JP (1) JP2505963B2 (ko)
KR (1) KR930008462A (ko)
AU (1) AU647953B2 (ko)
CA (1) CA2077014A1 (ko)
FI (1) FI924548A (ko)

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GB9417864D0 (en) * 1994-09-06 1994-10-26 Sinvent As Cell separation
US6015681A (en) * 1995-07-28 2000-01-18 The United States Of America As Represented By The Secretary Of The Navy Rapid immunoassay for cariogenic bacteria
AUPN901296A0 (en) 1996-03-29 1996-04-26 University Of Melbourne, The Porphyromonas gingivalis antigens for the diagnosis and treatment of periodontitis
US6153394A (en) * 1998-09-18 2000-11-28 Board Of Trustees Operating Michigan State University Immunoassay for equine protozoal myeloencephalitis in horses
US6984497B2 (en) * 2002-04-05 2006-01-10 Bio-Rad Laboratories, Inc. Reducing non-specific binding in immunoassays performed on polymeric solid phases
JP2006503089A (ja) * 2002-10-15 2006-01-26 ザ レジェンツ オブ ザ ユニバーシティー オブ カリフォルニア 齲蝕原性細菌に特異的なモノクローナル抗体
US8592219B2 (en) 2005-01-17 2013-11-26 Gyros Patent Ab Protecting agent
US20090010819A1 (en) * 2004-01-17 2009-01-08 Gyros Patent Ab Versatile flow path
WO2006075966A1 (en) * 2005-01-17 2006-07-20 Gyros Patent Ab A versatile flow path
JP2008527371A (ja) * 2005-01-17 2008-07-24 ユィロス・パテント・アクチボラグ 微小流体輸送導管の中で両親媒性高分子物質と一緒に反応物を共輸送するための方法
JP5124211B2 (ja) * 2007-08-24 2013-01-23 サンスター株式会社 免疫クロマト検査における口腔内由来検体の処理方法
WO2014126579A1 (en) * 2013-02-15 2014-08-21 Syngenta Participations Ag A method of extraction of an enzyme from plant or animal tissue

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GB1560570A (en) * 1976-01-29 1980-02-06 Fisher Scientific Co Method of obtaining and using antigen from neisseria gonorrheae and products prepared therefrom
AR231590A1 (es) * 1981-04-29 1984-12-28 Ciba Geigy Ag Dispositivo de analisis inmunologico y procedimiento para obtenerlo
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Publication number Publication date
US5399484A (en) 1995-03-21
EP0537828A1 (en) 1993-04-21
AU2114892A (en) 1993-04-22
FI924548A0 (fi) 1992-10-08
AU647953B2 (en) 1994-03-31
JP2505963B2 (ja) 1996-06-12
FI924548A (fi) 1993-04-09
CA2077014A1 (en) 1993-04-09
JPH05223821A (ja) 1993-09-03

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