KR930000530A - 말토올리고시드 유도체, 이것을 유효 성분으로 함유하는 α-아밀라아제 활성 측정용 시약 및 이것을 사용한 α-아밀라아제 활성의 측정 방법 - Google Patents

말토올리고시드 유도체, 이것을 유효 성분으로 함유하는 α-아밀라아제 활성 측정용 시약 및 이것을 사용한 α-아밀라아제 활성의 측정 방법 Download PDF

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KR930000530A
KR930000530A KR1019920011239A KR920011239A KR930000530A KR 930000530 A KR930000530 A KR 930000530A KR 1019920011239 A KR1019920011239 A KR 1019920011239A KR 920011239 A KR920011239 A KR 920011239A KR 930000530 A KR930000530 A KR 930000530A
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쇼오이찌 도꾸다께
다다시 도미꾸라
가즈오 고다니
가즈노리 사이또오
고오이찌로 도베
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나까노 고오자부로
깃꼬오망 가부시기가이샤
오까노 아쓰지
다이이찌 가가꾸 야꾸힝 가부시기가이샤
나스노 세이이찌
세이싱 세이야꾸 가부시기가이샤
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H15/00—Compounds containing hydrocarbon or substituted hydrocarbon radicals directly attached to hetero atoms of saccharide radicals
    • C07H15/20—Carbocyclic rings
    • C07H15/203—Monocyclic carbocyclic rings other than cyclohexane rings; Bicyclic carbocyclic ring systems
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H11/00—Compounds containing saccharide radicals esterified by inorganic acids; Metal salts thereof
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    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C07H17/06—Benzopyran radicals
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    • C07H17/075—Benzo[b]pyran-2-ones
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
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    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases

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Abstract

내용 없음

Description

말토올리고시드 유도체, 이것을 유효 성분으로 함유하는 α-아밀라아제 활성 측정용 시약 및 이것을 사용한 α-아밀라아제 활성의 측정 방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 실시예 5에 있어서 α-아밀라아제 활성의 측정에 사용된 검량선의 그래프.
제2도는 실시예 6에 있어서 α-아밀라아제 활성의 측정에 사용된 검량선의 그래프.
제3도는 실시예 9에 있어서 본 발명의 기질과 대조 기질과의 측정계내에서의 안정성을 나타내는 그래프.

Claims (7)

  1. 아래의 일반 구조식으로 나타내어지는 말토올리고시드 유도체.
    위의 식에서 n은 3∼5의 정수이고, R은 방향족 발색성 그룹이며, X는 〉CHCH2N3또는 〉C=CH2의 그룹이고, Y는 수소 원자, 치환 또는 비치환의 탄화 수소 그룹 또는 알킬-혹은 아릴술포닐 그룹이다.
  2. 제1항에 있어서, 방향족 발색성 그룹 R은 아래의 일반 구조식들로 나타내어지는 것인 말토올리고시드 유도체.
    위의 식에서, R1∼R5는 같거나 상이하며, 수소 원자, 할로겐 원자, 니트로 그룹, 알킬 그룹, 아릴 그룹, 아랄킬 그룹, 아미노 그룹, 술폰산 그룹 또는 카르복실 그룹이고, 또는 R1과 R2혹은 R2와 R3은 함께 결합하여 축합 방향족환을 형성하여도 좋다.
    (위의 식에서 R6은 수소 원자 또는 알킬 그룹이다)
    (위의 식에서 R7은 수소 원자 또는 할로겐 원자이다)
    (위의 식에서 R8∼R15는 같거나 상이하며, 수소 원자, 할로겐 원자, 니트로 그룹, 알킬 그룹, 아릴 그룹, 아릴킬 그룹, 아미노 그룹, 술폰산 그룹 또는 카르복실 그룹이고, R8과 R9혹은 R10과 R11은 함께 결합하여 축합 방향족환을 형성하여도 좋으며, R9및 R10및/또는 R13과 R14는 각각 공통의 산소 원자가 되어 축합 에테르환을 형성하여도 좋고, Z는 질소 원자 또는 N→0이다).
  3. 제1항에 있어서, 2-클로로-4-니트로페닐 65-아지도-65-데옥시-D-말토펜타오시드, 2-클로로-4-니트로페닐 65-아지도-66-데옥시-45-0-메실-D-말토펜타오시드, 2-클로로-4-니트로페닐 55-에노-D-말토펜타오시드, 2-클로로-4-니트로페닐 55-에노-45-0-메실-D-말토펜타오시드, 4-니트로페닐 57-에노-47-0-메톡시메틸-D-말토펜타오시드, 4-니트로페닐 67-아지도-67-데옥시-D-말토헵타오시드, 2, 4-디클로로페닐 67-아지도-67-데옥시-47-0-토실-D-말토헵타오시드, 페놀인도-3′-클로로페닐 65-아지도-65-데옥시-45-0-메틸-D-말토펜타오시드, 4-메틸움벨리페로닐 65-아지도-65-데옥시-D-말토펜타오시드, 레자주리닐 56-에노-D-말토헥사오시드, 루시페리닐 67-아지도-67-데옥시-47-0-알릴-D-말토헵타오시드 또는 페놀인도-3′-클로로페닐 55-에노-45-0-(2-메톡시)에톡시메틸-D-말토펜타오시드인 말토펜타오시드 유도체.
  4. 제1항에 있어서, 2-클로로-4-니트로페닐 65-아지도-65-데옥시-D-말토펜타오시드 또는 2-클로로-4-니트로페닐 55-에노-45-0-메실-D-말토펜타오시드인 말토펜타오시드 유도체.
  5. 제1항에 의한 말토올리고시드 유도체를 유효 성분으로 함유하는 α-아밀라아제 활성 측정용 시약.
  6. α-아밀라아제 함유 시료에 제1항에 의한 말토올리고시드 유도체의 α-아노머와 α-글루코시다아제 및/또는 글루코아밀라아제를 첨가하여 효소 반응을 시켜 유리하는 방향족 발색성 화합물을 정량함을 특징으로 하는 α-아밀라아제의 활성 측정 방법.
  7. α-아밀라아제 함유 시료에 제1항에 의한 말토올리고시드 유도체의 β-아노머 또는 α-아노머와 β-아노머와의 혼합물과 α-글루코시다아제 및/또는 글루코아밀라아제 및 β-글루코시다아제를 첨가하여 효소 반응을 시켜 유리하는 방향족 발색성 화합물을 정량함을 특징으로 하는 α-아밀라아제의 활성 측정 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019920011239A 1991-06-26 1992-06-26 말토올리고시드 유도체, 이것을 유효 성분으로 함유하는 알파-아밀라아제 활성측정용 시약 및 이것을 사용한 알파-아밀라아제 활성의 측정방법 KR0168864B1 (ko)

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JP91-180473 1991-06-26
JP3180473A JP2770891B2 (ja) 1991-06-26 1991-06-26 マルトオリゴシド誘導体、これを有効成分とするα‐アミラーゼ活性測定用試薬及びこれを用いたα‐アミラーゼ活性の測定方法

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JP (1) JP2770891B2 (ko)
KR (1) KR0168864B1 (ko)
DE (1) DE4220984A1 (ko)
GB (1) GB2257139B (ko)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101038939B1 (ko) * 2008-07-30 2011-06-03 정영환 알카리수 제조기

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JP3124435B2 (ja) * 1993-10-20 2001-01-15 キッコーマン株式会社 α‐アミラーゼアイソザイム活性の分別定量法
AU2001285392A1 (en) * 2000-07-31 2002-02-13 The Government of United States of America, as reprtesented by The Secretary, Department of Health And Human Services Macromolecular enzyme substrates
JP4553181B2 (ja) * 2004-03-25 2010-09-29 株式会社サタケ 残留農薬分析方法

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101038939B1 (ko) * 2008-07-30 2011-06-03 정영환 알카리수 제조기

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GB9212529D0 (en) 1992-07-22
US5302514A (en) 1994-04-12
DE4220984A1 (de) 1993-01-14
JP2770891B2 (ja) 1998-07-02
GB2257139A (en) 1993-01-06
JPH051091A (ja) 1993-01-08
KR0168864B1 (ko) 1999-01-15

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