KR920702415A - 안정화 고정화 효소 - Google Patents
안정화 고정화 효소Info
- Publication number
- KR920702415A KR920702415A KR1019920700925A KR920700925A KR920702415A KR 920702415 A KR920702415 A KR 920702415A KR 1019920700925 A KR1019920700925 A KR 1019920700925A KR 920700925 A KR920700925 A KR 920700925A KR 920702415 A KR920702415 A KR 920702415A
- Authority
- KR
- South Korea
- Prior art keywords
- enzyme
- stabilizing
- substance
- immobilized
- immobilized enzyme
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 시험예 1에 있어서의 결과를 나타낸다.는 실시예 1에서 얻은 본 발명물질을 나타내고,는 같은 방법에 의해 모라노린만을 첨가하지 않고 조제한 물질을 각기 나타낸다. 종축은 용액의 흡광도, 횡축은 경과시간(시간)을 각기 나타낸다. 제2도는 시험예 2에 있어서의 결과를 나타낸다.는 실시예 1에서 얻은 본 발명물질을 나타내고,는 같은 방법에 의해 모라노린만을 첨가하지 않고 조제한 물질을 각기 나타낸다. 종축은 활성 잔존율, 횡축은 온도(℃)를 각기 나타낸다.
Claims (3)
- 담체의 표면에 효소를 고정시켜서 이루어진 고정화 효소이며 그 대상이 당질 관련 효소인 것에 있어서, 당해 효소를 안정화시키는 작용이 있는 물질을 동시에 담체에 고정화시키는 것을 특징으로 하는 안정화 고정화 효소.
- 제1항에 있어서, 안정화시키는 작용이 있는 물질이 다음의 일반식(Ⅰ)로 표시되는 화합물 또는 이들의 글루코스올리고머인 안정화 고정화 효소:(상기 식중, R은 수소, 저급알킬, 하이드록시알킬, 페닐알킬, 페닐알케닐, 페닐알키닐, 페녹시알킬, 페녹시알케닐 또는 페녹시알키닐을 표시한다. 여기에서의 페닐이란 치환 또는 무치환의 것을 포함하는 것으로 한다. X는 소수 또는 축방향의 적도 방향의 수산기를 표시한다).
- 제1항에 있어서, 안정화 시키는 작용이 있는 물질이 α-시클로덱스트린인 안정화 고정화 효소.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP28704989 | 1989-11-01 | ||
JP89-287049 | 1989-11-01 | ||
JP90-185919 | 1990-07-13 | ||
JP18591990 | 1990-07-13 | ||
PCT/JP1990/001395 WO1991006640A1 (fr) | 1989-11-01 | 1990-10-31 | Enzyme immobilisee stabilisee |
Publications (1)
Publication Number | Publication Date |
---|---|
KR920702415A true KR920702415A (ko) | 1992-09-04 |
Family
ID=26503417
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019920700925A KR920702415A (ko) | 1989-11-01 | 1990-10-31 | 안정화 고정화 효소 |
Country Status (7)
Country | Link |
---|---|
EP (1) | EP0498889A4 (ko) |
KR (1) | KR920702415A (ko) |
CN (1) | CN1051587A (ko) |
AU (1) | AU643469B2 (ko) |
CA (1) | CA2072616A1 (ko) |
HU (1) | HUT63876A (ko) |
WO (1) | WO1991006640A1 (ko) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
TW383336B (en) * | 1993-06-24 | 2000-03-01 | Consortium Elektrochem Ind | Cyclodextrin glycosyl transferases for the preparation of gama-cyclodextrin |
WO2001004631A1 (en) * | 1999-07-08 | 2001-01-18 | Radiometer Medical A/S | A sensor comprising a hydrophilic matrix material |
DE102006006439B4 (de) † | 2006-02-13 | 2008-11-27 | Wabco Gmbh | Ventileinrichtung zur manuellen Veränderung der Niveaulage eines luftgefederten Fahrzeuges |
CN108918625B (zh) * | 2018-07-27 | 2020-11-24 | 三诺生物传感股份有限公司 | 一种生物传感膜的制备方法、生物传感膜及监测装置 |
CN110283809A (zh) * | 2019-06-28 | 2019-09-27 | 苏州大学 | 一种β-葡糖苷酶固定化方法及应用 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5215890A (en) * | 1975-07-24 | 1977-02-05 | Unitika Ltd | Method for immobilizing fibrinolysokinases |
EP0039104B1 (fr) * | 1980-04-25 | 1983-05-11 | Etat belge représenté par l'Exécutif de la Région Wallonne | Procédé d'immobilisation de la bêta-galactosidase et produits ainsi obtenus |
US4652524A (en) * | 1980-10-02 | 1987-03-24 | Ivan E. Modrovich | Soluble stabilized enzymes |
DE3265377D1 (en) * | 1981-05-11 | 1985-09-19 | Tate & Lyle Plc | Immobilized enzymes |
SE454597B (sv) * | 1984-04-02 | 1988-05-16 | Erik Dahlgren | Enzympapper for indikering av kolinesterashemmare der enzymet immobiliserats av en anjonbytare, forfarande for dess framstellning samt anvendning av pappret med ett substrat |
JPS61170389A (ja) * | 1985-01-21 | 1986-08-01 | Fuji Electric Co Ltd | 固定化酵素膜の製造法 |
JPS62248487A (ja) * | 1986-04-22 | 1987-10-29 | Dentaru Kagaku Kk | グルカナーゼを固定化したアパタイトおよびその製造法 |
US5256788A (en) * | 1989-02-13 | 1993-10-26 | Nippon Shinyaku Co. Ltd. | Moranoline derivatives and their production and the use of moranoline and its derivatives as a stabilizing agent for enzymes |
-
1990
- 1990-10-31 AU AU66296/90A patent/AU643469B2/en not_active Ceased
- 1990-10-31 KR KR1019920700925A patent/KR920702415A/ko not_active Application Discontinuation
- 1990-10-31 EP EP19900916059 patent/EP0498889A4/en not_active Ceased
- 1990-10-31 CN CN90108886A patent/CN1051587A/zh active Pending
- 1990-10-31 WO PCT/JP1990/001395 patent/WO1991006640A1/ja not_active Application Discontinuation
- 1990-10-31 HU HU9200989A patent/HUT63876A/hu unknown
- 1990-10-31 CA CA002072616A patent/CA2072616A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
WO1991006640A1 (fr) | 1991-05-16 |
AU6629690A (en) | 1991-05-31 |
HUT63876A (en) | 1993-10-28 |
AU643469B2 (en) | 1993-11-18 |
HU9200989D0 (en) | 1992-06-29 |
CA2072616A1 (en) | 1991-05-02 |
EP0498889A1 (en) | 1992-08-19 |
CN1051587A (zh) | 1991-05-22 |
EP0498889A4 (en) | 1993-05-05 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |