KR920004578A - Method for producing citric acid using citrus peel - Google Patents

Method for producing citric acid using citrus peel Download PDF

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Publication number
KR920004578A
KR920004578A KR1019900013739A KR900013739A KR920004578A KR 920004578 A KR920004578 A KR 920004578A KR 1019900013739 A KR1019900013739 A KR 1019900013739A KR 900013739 A KR900013739 A KR 900013739A KR 920004578 A KR920004578 A KR 920004578A
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KR
South Korea
Prior art keywords
citric acid
citrus peel
gfm
incubated
hours
Prior art date
Application number
KR1019900013739A
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Korean (ko)
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KR930001261B1 (en
Inventor
강신권
최장윤
권익부
Original Assignee
정보영
롯데제과 주식회사
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Application filed by 정보영, 롯데제과 주식회사 filed Critical 정보영
Priority to KR1019900013739A priority Critical patent/KR930001261B1/en
Publication of KR920004578A publication Critical patent/KR920004578A/en
Application granted granted Critical
Publication of KR930001261B1 publication Critical patent/KR930001261B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/48—Tricarboxylic acids, e.g. citric acid
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/913—Aspergillus
    • Y10S435/917—Aspergillus niger

Abstract

내용 없음No content

Description

감귤과피를 이용한 구연산의 제조 방법Method for producing citric acid using citrus peel

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음As this is a public information case, the full text was not included.

제1도는 pH가 구연산의 생산에 미치는 영향을 나타낸 도면,1 is a diagram showing the effect of pH on the production of citric acid,

제2도는 액체배양시 시간경과에 따른 구연산의 생산량을 나타낸 도면,2 is a view showing the amount of citric acid production according to the passage of time in liquid culture,

제3도는 고체배양시 시간경과에 따른 구연산의 생산량을 온도별로 나타낸 도면이다.3 is a diagram showing the amount of citric acid produced by temperature over time in solid culture.

Claims (3)

감귤과피를 발효시켜 구연산을 제조함에 있어서, 15∼30메쉬의 입자크기로 분쇄시킨 감귤과피에다, 이와 같은 중량%의 물을 가하고, 여기에 NH4NO20.2%, MgSO4·7H2O 0.1% 메탄올 2%를 첨가하여 pH4-5로 조절한 다음, 여기에다 분리균 아스퍼질루스 나이가 GFM-0014를 접종하여 10 내지 35℃온도에서 3일간 배양한 후, 그 배양물로부터 구연산을 분리 정제해내는 것을 특징으로 하는 감귤과피를 이용한 구연산의 제조방법.In preparing citric acid by fermenting citrus peel, to the citrus peel pulverized into a particle size of 15-30 mesh, such weight% of water is added, and NH 4 NO 2 0.2%, MgSO 4 · 7H 2 O 0.1 2% of methanol was added to adjust the pH to 4-5, followed by inoculation with the isolate B. Aspergillus AGE GFM-0014, incubated at 10-35 ° C. for 3 days, and the citric acid was separated and purified from the culture. Citric acid production method using citrus peel, characterized in that. 제1항에 있어서, 아스피질루스나이가 GFM-0014의 배양온도는 초기 20시간동안은 35℃, 중기 4시간은 10℃, 후기 48시간은 30℃의 온도에서 각각 배양함을 특징으로 하는 방법.The method according to claim 1, wherein the aspiryl scavenger cultivation temperature of GFM-0014 is incubated at a temperature of 35 ° C. for the first 20 hours, 10 ° C. for the middle 4 hours, and 30 ° C. for the latter 48 hours. . 감귤과피를 발효시켜 구연산을 제조함에 있어서, 미세하게 분쇄시킨 감귤과피 6중량%에다 100중량%의 물을 가하고, 여기에 NH4NO20.2%, MgSO4·7H2O 0.1%, 메탄올 3%를 첨가하여 pH4-5로 조절한 다음, 여기에다 분리균 아스피질루스나이가 GFM-0014를 접종하여 30℃온도에서 7일간 배양한후, 그 배양물로부터 구연산을 분리정제해내는 것을 특징으로 하는 감귤과피를 이용한 구연산의 제조방법.In preparing citric acid by fermenting citrus peel, 100% by weight of water is added to 6% by weight of finely ground citrus peel, and 0.2% NH 4 NO 2 , 0.1% MgSO 4 · 7H 2 O, and 3% methanol. It was adjusted to pH 4-5 by adding to it, and then isolated by inoculation with GFM-0014 and incubated for 7 days at 30 ° C., and then citric acid was separated and purified from the culture. Method for producing citric acid using skin. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019900013739A 1990-08-31 1990-08-31 Process for preparing citric acid by tangerin peelings KR930001261B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
KR1019900013739A KR930001261B1 (en) 1990-08-31 1990-08-31 Process for preparing citric acid by tangerin peelings

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019900013739A KR930001261B1 (en) 1990-08-31 1990-08-31 Process for preparing citric acid by tangerin peelings

Publications (2)

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KR920004578A true KR920004578A (en) 1992-03-27
KR930001261B1 KR930001261B1 (en) 1993-02-22

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Application Number Title Priority Date Filing Date
KR1019900013739A KR930001261B1 (en) 1990-08-31 1990-08-31 Process for preparing citric acid by tangerin peelings

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100892359B1 (en) * 2008-05-13 2009-04-08 (주)큐젠바이오텍 Method for production of scleroglucan through cultivation of sclerotium sp. in culture medium including mandarin peels as carbon sources
KR101406044B1 (en) * 2007-06-08 2014-06-12 한국생산기술연구원 Production of coenzyme Q10 through cultivation of Rhodotorulla sp. in culture medium adding mandarin peels as carbon source
KR102566260B1 (en) * 2022-08-01 2023-08-14 (주)바이오웍스 Manufacturing method of febric deodorant composition

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100888226B1 (en) * 2007-08-28 2009-03-12 주식회사농심 Fat soluble cirtic acid, the preparation method thereof and the oil containing the fat soluble citric acid

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101406044B1 (en) * 2007-06-08 2014-06-12 한국생산기술연구원 Production of coenzyme Q10 through cultivation of Rhodotorulla sp. in culture medium adding mandarin peels as carbon source
KR100892359B1 (en) * 2008-05-13 2009-04-08 (주)큐젠바이오텍 Method for production of scleroglucan through cultivation of sclerotium sp. in culture medium including mandarin peels as carbon sources
KR102566260B1 (en) * 2022-08-01 2023-08-14 (주)바이오웍스 Manufacturing method of febric deodorant composition

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Publication number Publication date
KR930001261B1 (en) 1993-02-22

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