KR970001555A - Method for producing microbial cellulose from fruit juice - Google Patents

Method for producing microbial cellulose from fruit juice Download PDF

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Publication number
KR970001555A
KR970001555A KR1019960049979A KR19960049979A KR970001555A KR 970001555 A KR970001555 A KR 970001555A KR 1019960049979 A KR1019960049979 A KR 1019960049979A KR 19960049979 A KR19960049979 A KR 19960049979A KR 970001555 A KR970001555 A KR 970001555A
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South Korea
Prior art keywords
microbial cellulose
fruit juice
cellulose
producing
present
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KR1019960049979A
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Korean (ko)
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KR100202441B1 (en
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변유량
양영국
박상훈
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변유량
양영국
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 아세토박터(Acetobacter) 균주를 이용하여 초모(starter)를 제조하고, 그를 이용하여 과일쥬스로부터 미생물 셀룰로오스를 제조하는 방법에 관한 것이다. 본 발명의 미생물 셀룰로오스 제조방법은 (ⅰ) 초모(starter) 제조용 배지에 미생물 셀룰로오스를 생산하는 미생물을 접종하고, 진탕배양하여 초모를 수득하는 공정; (ⅱ) 전기에서 수득한 초모를 과일쥬스에 접종하고 정치배양하여, 미생물 셀룰로오스를 생산하는 공정; 및, (ⅲ) 전기에서 생산된 미생물 셀룰로오스를 후처리, 절단, 포장 및 살균하는 공정을 포함한다. 본 발명에 의해 제조된초모는 입자(particle) 또는 침전물(pellet)의 분산 형태로 된 미생물 셀룰로오스 배양액이므로, 과일로부터 생산되는 미생물 셀룰로오스 배양에의 접종균으로 사용이 용이하고, 동일 품질의 미생물 셀룰로오스를 얻을 수 있다. 또한, 본 발명의 방법으로 제조된 미생물 셀룰로오스는 인체내에서 소화 흡수되지 않는 섬유질을 주성분으로 하고, 물 흡수, 유기물 흡착, 금속이온 흡착 및 겔 형성과 같은 생리작용을 하기 때문에, 식물 섬유가 결핍된 식사 또는 적은 식사를 하는 현대인에게서 유발되는 장 질환인 변비, 맹장염과 대장암 등이나, 대사성 질환인 비만과 당뇨병 등에 예방 효과가 있을 뿐만 아니라, 다이어트 및 건강 식품으로도 유용하게 사용될 수 있다.The present invention relates to a method for producing a starter using acetobacter strain, and to produce microbial cellulose from fruit juice using the same. Microbial cellulose production method of the present invention is (i) inoculating the microorganisms producing microbial cellulose in a medium for preparing the starting hair (starter), shaking cultured to obtain a superfiber; (Ii) inoculating the seedlings obtained in the past into fruit juice and incubating them to produce microbial cellulose; And (iii) post-treatment, cutting, packaging, and sterilizing the microbial cellulose produced in the past. The superfiber prepared by the present invention is a microbial cellulose culture medium in the form of dispersed particles or pellets, so it is easy to use as an inoculation bacterium in the microbial cellulose culture produced from fruit, and microbial cellulose of the same quality is used. You can get it. In addition, the microbial cellulose produced by the method of the present invention is based on the fiber that is not digested and absorbed in the human body, and because the physiological action such as water absorption, organic material adsorption, metal ion adsorption and gel formation, the plant fiber is deficient As well as preventive effects such as constipation, appendicitis and colorectal cancer, or metabolic diseases such as obesity and diabetes, which are caused by modern people eating or eating less, it can be useful as a diet and health food.

Description

과일쥬스로부터 미생물 셀룰로오스의 제조방법Method for producing microbial cellulose from fruit juice

본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음Since this is an open matter, no full text was included.

Claims (7)

(ⅰ) 초모(starter) 제조용 배지에 미생물 셀룰로오스를 생산하는 미생물을 접종하고, 진탕배양하여 초모를 수득하는 공정; (ⅱ) 전기에서 수득한 초모를 과일쥬스에 접종하고 정치배양하여, 미생물 셀룰로오스를 생산하는 공정 ; 및, (ⅲ) 전기에서 생산된 미생물 셀룰로오스를 후처리, 절단, 포장 및 살균하는 공정을 포함하는 과일쥬스로부터의 미생물 셀룰로오스 제조방법.(Iii) inoculating microorganisms producing microbial cellulose in a medium for producing supernatant and shaking culture to obtain supercaps; (Ii) a step of inoculating the seedlings obtained in the past with fruit juice and incubating them to produce microbial cellulose; And (iii) post-treatment, cutting, packaging, and sterilizing the microbial cellulose produced in the past. 제1항에 있어서, 초모 제조용 배지는 초산을 포함한 과일쥬스 또는 펩톤(옥소이드) 5%, 효모추출액(옥소이드) 5%, Na2HPO42.7%, 구연산 1.15% 및 글루코오스 20.0%로 구성된 배지(pH 6.0)인 것을 특징으로 하는 과일쥬스로부터의 미생물 셀룰로오스 제조방법.The method of claim 1, wherein the culture medium for chondroitin is composed of 5% fruit juice or peptone (oxoid) including acetic acid, 5% yeast extract (oxoid), 2.7% Na 2 HPO 4 , 1.15% citric acid and 20.0% glucose. (pH 6.0) The method for producing microbial cellulose from fruit juice. 제1항에 있어서, 미생물 셀룰로오스를 생산하는 미생물은 아세토박터(Acetobacter) 속의 균주인 것을 특징으로 하는 과일쥬스로부터의 미생물 셀룰로오스 제조방법.The method of claim 1, wherein the microorganism producing microbial cellulose is a strain of the genus Acetobacter (Acetobacter) production method of microbial cellulose from fruit juice. 제1항에 있어서, 진탕배양은 생산되는 미생물 셀룰로오스가 입자(particle) 또는 침전물(pellet) 형태의분산 상태로 되도록 하는 것을 특징으로 하는 과일쥬스로부터의 미생물 셀룰로오스 제조방법.2. The method of claim 1, wherein the shaking culture is such that the microbial cellulose produced is dispersed in the form of particles or pellets. 제1항에 있어서, 초모는 진탕배양액 자체이거나, 진탕배양액에서 셀룰레이즈를 첨가하여 셀룰로오스를 제거하고 원심분리하여 수득한 균주로 하는 것을 특징으로 하는 과일쥬스로부터의 미생물 셀룰로오스 제조방법.The method for producing microbial cellulose from fruit juice according to claim 1, wherein the chondroitus is a shake culture solution itself, or a strain obtained by adding cellulose in the shake culture solution to remove cellulose and centrifuging. 제1항에 있어서, 초모를 과일쥬스에 3 내지 8%(v/v)로 접종하고, 25 내지 35℃에서 5 내지 20일 동안정치배양하는 것을 특징으로 하는 과일쥬스로부터의 미생물 셀룰로오스 제조방법.The method for producing microbial cellulose from fruit juice according to claim 1, wherein the seedlings are inoculated at 3 to 8% (v / v) in the fruit juice and cultured for 5 to 20 days at 25 to 35 ° C. 제1항에 있어서, 과일쥬스는 사과쥬스인 것을 특징으로 하는 과일쥬스로부터의 미생물 셀룰로오스 제조방법.2. The method of claim 1, wherein the fruit juice is apple juice. ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.※ Note: The disclosure is based on the initial application.
KR1019960049979A 1996-10-30 1996-10-30 Manufacturing process of microbial cellulose from fruit juice KR100202441B1 (en)

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KR970001555A true KR970001555A (en) 1997-01-24
KR100202441B1 KR100202441B1 (en) 1999-06-15

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Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020080802A (en) * 2001-04-17 2002-10-26 변유량 Method for Manufacturing Microbial Cellulose Employing Soybean Processed Product
KR101137766B1 (en) 2010-07-07 2012-06-27 대한민국 A novel strain of Gluconacetobacter sp. gel_SEA623-2 from citrus juice and cellulose gel by using it
KR101814538B1 (en) 2016-04-21 2018-01-03 휴코스코리아(주) A cosmentic composition comprising the bio-cellulose powder for reducing and preventing cellulite
KR101814537B1 (en) 2016-04-21 2018-01-03 휴코스코리아(주) A skin topical composition comprising the bio-cellulose powder for reducing and preventing cellulite

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