KR910700347A - 프로모터, 그를 함유하는 플라스미드 및 재조합 아비폭스비루스 - Google Patents

프로모터, 그를 함유하는 플라스미드 및 재조합 아비폭스비루스 Download PDF

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KR910700347A
KR910700347A KR1019900701934A KR900701934A KR910700347A KR 910700347 A KR910700347 A KR 910700347A KR 1019900701934 A KR1019900701934 A KR 1019900701934A KR 900701934 A KR900701934 A KR 900701934A KR 910700347 A KR910700347 A KR 910700347A
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nucleotide sequence
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virus
abipoxvirus
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료헤이 오가와
노보루 야나기다
사끼꼬 사에끼
세쓰꼬 오오까와
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다끼시와 다께시
닛뽕 제온 가부시끼가이샤
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Abstract

내용 없음.

Description

프로모터, 그를 함유하는 플라스미드 및 재조합 아비폭스비루스
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1,2,3도는 본 발명에 따라 프로모터 활성을 갖는 단편 14-6, 49-22, 41-3 및 37-1의 뉴클레오티드 서열을 나타낸다.

Claims (12)

  1. 프로모터 활성을 갖는 제1,2,3 또는 4도에 나타낸 뉴클레오티드 서열의 일부 또는 전부를 함유하는 뉴클레오티드 서열.
  2. 제1항에 있어서, 뉴클레오티드 서열이 제4도에 나타낸 뉴클레오티드 서열중 404~515 뉴클레오티드의 일부 또는 전부를 함유하는 것인 뉴클레오티드 서열.
  3. 제2항에 있어서, 뉴클레오티드 서열이 제4도에 나타낸 뉴클레오티드 서열중 489~505 뉴클레오티드의 일부 또는 전부를 함유하는 것인 뉴클레오티드 서열.
  4. 제1항 내지 3항중 어느 한 항의 뉴클레오티드 서열, 및 상기 뉴클레오티드 서열 지배하에 있는, 폴리펩티드를 코팅하는 외인성 유전자를 함유하는 키메라9chimera) 유전자.
  5. 제4항에 있어서, 상기 외인성 유전자가 뉴카슬병(New-castle disease) 비루스 항원을 코딩하는 유전자인 키메라 유전자.
  6. 제5항에 있어서, 상기 뉴카슬병 비루스 항원이 헤마글루미닌 뉴라미니다제(hemagglutinin neuraminidase)인 키메라 유전자.
  7. 제4항 내지 6항중 어느 한 항에 따른 키메라 유전자를 함유하며 에스케리키아콜리(Escherichia coli)내에서 증식할 수 있는 플라스미드.
  8. 제7항에 있어서, 상기 키메라 유전자가 아비폭스비루스(Avipoxvirus)의 증식에 비-필수적인 게놈 영역에서 유래된 뉴클레오티드 서열내에 삽입된 플라스미드.
  9. 아비폭스비루스의 증식에 비-필수적인 게놈 영역내에 삽입된, 제4항 내지 6항중 어느 한 항에 따른 키메라 유전자를 함유하는 재조합 아비폭스비루스.
  10. 제9항에 있어서, 상기 아비폭스비루스가 파울폭스비루스(fowlpoxvirus)인 재조합 아비폭스비루스.
  11. 프로모터 지배하에 있지 않은 마커 유전자를 함유하는 플라스미드의 마키 유전자의 상류에 비루스-유래 DNA 단면을 삽입하고, 상기 플라스미드를 사용하여 재조합 비루스를 제작하고, 재조합 비루스로 마커 유전자-삭제 세포를 감염시키고, 마키 유전자가 발현되는 세포를 선택하고, 및 삽입된 DNA 단면의 뉴클레오티드 서열을 분석함을 특징으로 하는, 프로모터 활성을 갖는 뉴클레오티드 서열의 선택방법.
  12. 제10항에 있어서, 상기 마커 유전자가 1acz 유전자인 프로모터 활성을 갖는 뉴클레오티드 서열의 선택방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019900701934A 1988-12-29 1989-12-28 프로모터, 그를 함유하는 플라스미드 및 재조합 아비폭스비루스 KR940001265B1 (ko)

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JP33560588 1988-12-29
JP63-335605 1988-12-29
JP1-76025 1989-03-28
JP7602589 1989-03-28
PCT/JP1989/001330 WO1990007581A1 (fr) 1988-12-29 1989-12-28 Promoteur, plasmide le contenant, et avipoxvirus de recombinaison

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KR910700347A true KR910700347A (ko) 1991-03-14
KR940001265B1 KR940001265B1 (ko) 1994-02-18

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EP (1) EP0419666A4 (ko)
KR (1) KR940001265B1 (ko)
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WO (1) WO1990007581A1 (ko)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0353851B1 (en) * 1988-06-24 1994-01-19 Btg International Limited Fowlpox virus non-essential regions
JP2781605B2 (ja) * 1989-06-22 1998-07-30 日本ゼオン株式会社 組み換えアビポックスウイルス
KR930701588A (ko) * 1990-07-02 1993-06-12 스튜어트 알. 슈터 재조합 구두 바이러스 백신
BE1004877A3 (fr) * 1991-05-27 1993-02-16 Solvay Virus de l'avipox recombinant, culture de cellules infectees par ce virus et vaccins pour la volaille derives de ce virus.

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61170393A (ja) * 1985-01-21 1986-08-01 Nippon Zeon Co Ltd 組換え体ベクタ−及びその製造法
JPS61271990A (ja) * 1985-05-27 1986-12-02 Agency Of Ind Science & Technol 改造ジヒドロ葉酸還元酵素遺伝子を含む特定ｄｎａ配列
WO1988002022A1 (en) * 1986-09-22 1988-03-24 Commonwealth Scientific And Industrial Research Or Recombinant poxviruses
DE3853088T2 (de) * 1987-03-27 1995-10-19 Nippon Zeon Co Rekombinantes Avipoxvirus.
CA1340203C (en) * 1987-09-16 1998-12-15 Noboru Yanagida Recombinant apivoxvirus
EP0353851B1 (en) * 1988-06-24 1994-01-19 Btg International Limited Fowlpox virus non-essential regions
CA2001001A1 (en) * 1988-10-21 1990-04-21 Matthew M. Binns Fowlpox virus promoter

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EP0419666A1 (en) 1991-04-03
WO1990007581A1 (fr) 1990-07-12
EP0419666A4 (en) 1991-05-15
KR940001265B1 (ko) 1994-02-18
AU623476B2 (en) 1992-05-14
AU4824090A (en) 1990-08-01

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