KR950005994A - 곤충세포를 이용한 씨(C)형 간염바이러스의 핵(Core22) 단백질의 제조 - Google Patents

곤충세포를 이용한 씨(C)형 간염바이러스의 핵(Core22) 단백질의 제조 Download PDF

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KR950005994A
KR950005994A KR1019930016866A KR930016866A KR950005994A KR 950005994 A KR950005994 A KR 950005994A KR 1019930016866 A KR1019930016866 A KR 1019930016866A KR 930016866 A KR930016866 A KR 930016866A KR 950005994 A KR950005994 A KR 950005994A
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core22
virus
expression vector
hepatitis
insect cells
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KR100321444B1 (ko
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조중명
김성택
소홍섭
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최근선
주식회사 럭 키
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8294—Auxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N2710/14011—Baculoviridae
    • C12N2710/14041—Use of virus, viral particle or viral elements as a vector
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    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24211—Hepacivirus, e.g. hepatitis C virus, hepatitis G virus
    • C12N2770/24222—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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Abstract

본 발명은 한국형 C형 간염바이러스 Core22 유전자 (KHCV LBC1의 346번째에서 915번째 염기까지의 유전자)를 곤충세포에서 발현시키기 위한 발현벡터, 이 발현벡터로 형질전화된 숙주세포 및 이를 이용하여 한국형 C형 감염바이러스의 Core22핵 단백질을 제조하는 방법에 관한 것이다.

Description

곤충세포를 이용한 씨(C)형 간염바이러스의 핵(Core22) 단백질의 제조
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 한국형의 C형 간염바이러스의 Core22핵 단백질의 뉴클레오티드 서열 및 아미노산 서열을 나타낸 것이고,
제2도는 한국형 C형 간염바이러스의 Core22핵 단백질 유전자를 베큘로 바이러스 발현베터 pAC360에 접합시키기 위한, 아미노 및 카복시 말단 프라이머를 나타낸 것이고,
제3도는 한국형 C형 간염바이러스의 Core22핵 단백질 유전자의 접합전략 및 발현벡터의 제조 과정을 나타낸 것이고,
제4도는 Core22 유전자를 함유하는 재조합 베큘로바이러스를 곤충세포에 감염시켜 핵단백질의 발현을 유도시킨 후 웨스턴 블롯팅에 의해 확인한 결과이다.

Claims (7)

  1. 한국형 C형 간염 바이러스의 Core22 유전자를 포함하는 재조합 베큘로바이러스 발현 벡터.
  2. 제1항에 있어서, 야생형 베큘로바이러스 DNA의 폴리히드린 유전자를 상기 Core22 유전자로 치환시킨 발현 벡터.
  3. 제2항에 있어서, Core22 단백질 유전자를 포함하는 재조합 베큘로바이러스 클로닝 벡터를 야생형 베큘로바이러스 DNA와 함께 곤충세포에 공형질감염시켜 제조한 발현 벡터.
  4. 제3항에 있어서, 상기 재조합 베큘로바이러스 클로닝 벡터가 pVC360 Core22인 발현 벡터.
  5. 제1항 내지 제4한 중 어느 한 항의 발현 벡터를 포함하는 재조합 베큘로바이러스.
  6. 제5항에 있어서, BVLUC-Core22(ATCC VR 2419)인 재조합 베큘로바이러스.
  7. 제5항 또는 제6항의 재조합 베큘로바이러스로 곤충세포를 감염시켜 배양함을 포함하는, 한국형 C형 간염 바이러스의 Core22핵 단백질의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019930016866A 1993-08-27 1993-08-27 곤충세포를이용한씨(C)형간염바이러스의핵(Core22)단백질의제조 KR100321444B1 (ko)

Priority Applications (1)

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KR1019930016866A KR100321444B1 (ko) 1993-08-27 1993-08-27 곤충세포를이용한씨(C)형간염바이러스의핵(Core22)단백질의제조

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KR1019930016866A KR100321444B1 (ko) 1993-08-27 1993-08-27 곤충세포를이용한씨(C)형간염바이러스의핵(Core22)단백질의제조

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KR950005994A true KR950005994A (ko) 1995-03-20
KR100321444B1 KR100321444B1 (ko) 2002-08-13

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