KR900017052A - 미생물 세포벽 용해 효소와 그의 생산 미생물 - Google Patents

미생물 세포벽 용해 효소와 그의 생산 미생물 Download PDF

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Publication number
KR900017052A
KR900017052A KR1019890004661A KR890004661A KR900017052A KR 900017052 A KR900017052 A KR 900017052A KR 1019890004661 A KR1019890004661 A KR 1019890004661A KR 890004661 A KR890004661 A KR 890004661A KR 900017052 A KR900017052 A KR 900017052A
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KR
South Korea
Prior art keywords
cell wall
microbial cell
genus
kfcc
producing microorganisms
Prior art date
Application number
KR1019890004661A
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English (en)
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KR910004363B1 (ko
Inventor
유주현
정명호
권익부
이윤수
Original Assignee
유주현
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 유주현 filed Critical 유주현
Priority to KR1019890004661A priority Critical patent/KR910004363B1/ko
Priority to JP2090252A priority patent/JPH0367579A/ja
Priority to DK088090A priority patent/DK88090A/da
Publication of KR900017052A publication Critical patent/KR900017052A/ko
Application granted granted Critical
Publication of KR910004363B1 publication Critical patent/KR910004363B1/ko
Priority to US07/974,326 priority patent/US5236839A/en

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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01C—RESISTORS
    • H01C10/00—Adjustable resistors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Microelectronics & Electronic Packaging (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

내용 없음.

Description

미생물 세포벽 용해 효소와 그의 생산 미생물
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제 1 도는 본 균주의 효소를 기질 균체가 도말된 플레이트(plate)에 스포팅(spotting)하여 용균 여부를 나타낸 사진이다.
제 2 도는 미생물 세포벽에 본 균주의 효소를 작용시켜 얻어진 반응 생성물을 분석한 도면이다.
제 3 도는 미생물 세포벽에 용해 효소를 생산하는 미생물의 전자 현미경 사진 도해이다.

Claims (3)

  1. 미생물 세포벽 용해 효소를 생산하는 호알칼리성 비실러스 속 KFCC 10671의 미생물.
  2. 호알칼리성 바실러스 속 KFCC 10671의 미생물을 pH 7.5∼11.5의 영양배지에서 배양하여 배양액으로부터 미생물 세포벽 용해 효소를 회수함을 특징으로 하는, 미생물 세포벽 용해 효소의 제조방법.
  3. 호알칼리성 바실러스 속 KFCC 10671가 생산하는, 하기의 특성을 갖는 미생물 세포벽 용해 효소.
    분자량 : 약 27,000 달톤
    최적 활성 pH : pH 10 근처
    반응 온도 : 40∼80℃
    최적반응 온도 : 50℃
    안정 pH : pH 5∼11
    최적안정 pH : pH 10
    내알칼리성
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890004661A 1989-04-08 1989-04-08 미생물 세포벽 용해 효소와 그의 생산 미생물 KR910004363B1 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1019890004661A KR910004363B1 (ko) 1989-04-08 1989-04-08 미생물 세포벽 용해 효소와 그의 생산 미생물
JP2090252A JPH0367579A (ja) 1989-04-08 1990-04-06 微生物細胞壁溶解酵素とその生産微生物
DK088090A DK88090A (da) 1989-04-08 1990-04-06 Mikrobielt lytisk cellevaegsenzym, dets fremstilling og ved fremstillingen anvendt mikroorganisme
US07/974,326 US5236839A (en) 1989-04-08 1992-11-10 Microbial cell wall lytic enzyme from Bacillus FERM BP-2841

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019890004661A KR910004363B1 (ko) 1989-04-08 1989-04-08 미생물 세포벽 용해 효소와 그의 생산 미생물

Publications (2)

Publication Number Publication Date
KR900017052A true KR900017052A (ko) 1990-11-15
KR910004363B1 KR910004363B1 (ko) 1991-06-26

Family

ID=19285213

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019890004661A KR910004363B1 (ko) 1989-04-08 1989-04-08 미생물 세포벽 용해 효소와 그의 생산 미생물

Country Status (3)

Country Link
JP (1) JPH0367579A (ko)
KR (1) KR910004363B1 (ko)
DK (1) DK88090A (ko)

Also Published As

Publication number Publication date
KR910004363B1 (ko) 1991-06-26
DK88090A (da) 1990-10-09
DK88090D0 (da) 1990-04-06
JPH0367579A (ja) 1991-03-22

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