KR900006520A - 결실 탐지를 위한 게놈성 dna다중 증폭방법 - Google Patents

결실 탐지를 위한 게놈성 dna다중 증폭방법 Download PDF

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KR900006520A
KR900006520A KR1019890014542A KR890014542A KR900006520A KR 900006520 A KR900006520 A KR 900006520A KR 1019890014542 A KR1019890014542 A KR 1019890014542A KR 890014542 A KR890014542 A KR 890014542A KR 900006520 A KR900006520 A KR 900006520A
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티.캐스케이 찰스
에스.챔버레인 제프리
에이.엘.깁스 리차드
이.레이너 조엘
느가 느구엔 피
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Abstract

내용 없음

Description

결실 탐지를 위한 게놈성 DNA다중 증폭방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 근사치의 좌우크기, 증폭된 단편의 위치, 및 클론화되고 서열분석된 게놈성 영역의 위치가 도해된 DMD유전자의 개요도이다.
제2도는 산전 진단을 위해 태아의 DNA에서의 결실을 탐지하는데 사용된 PCR반응의 예시도이다.
제3도는 관련이 없는 남자 DMD환자로부터 유래된 림프아세포의 DNA다중 증폭을 나타낸 것이다. A 및 B는 10개 샘플로 된 두세트를 보여준다. 각 DRL#는 알.제이.클레버그 센터프 휴먼제네틱스 다이아그노스틱 리써치 래버러토리(R.J.Kleberg Center for Fuman Genetics Diagnostic Research Laboratory)의 가계수를 의미한다. MW : Hae Ⅲ-절단한 ø X 174 DNA. (-) : 주형 DNA가 반응에 첨가되지 않았음. 증폭된 영역과 유전자상의 영역의 관계는 A의 우측에 나타낸다. 문자는 제1도에서와 상응하는 것이다.

Claims (17)

  1. 다수의 DNA서여을 처리하여 단일 상보본쇄를 형성하고 : 다수의 쌍을 이룬 올리고 뉴크레오티드 프라이머(primer)를 첨가하고(여기에서, 각 쌍은 다른 서열에 의해 특이적이며, 각 쌍의 한쪽 프라이머는 센스(sense)-본쇄내의 서열의 한부분에 실질적으로 상보적이고 각 쌍의 다른쪽 프라이머는 안티-센스(anti-sense) 상보본쇄 내의 동일 서열의 다른 부분에 실질적으로 상보적이다) ; 다수의 프라이머를 이들의 상보적인 서열에 어닐링(annealing)시키고 ; 다수의 어닐링된 프라이머를 각 프라이머의 3´말단으로부터 동시에 연장시켜 각 프라이머 어닐링된 본쇄에 상보적인 연장 생성물을 합성하고(여기에서, 여장 생성물은 이들의 상보본쇄로부터 분리된 후 각 쌍의 다른 프라이머로부터 연장 생성물의 합성을 위한 주형으로서 작용한다) ; 연장 생성물을 이들의 주형으로부터 분리하여 단일 본쇄 분자를 생성하고 ; 단일 본쇄 분자를 상기의 어닐링 단계, 연장 단계 및 분리 단계를 적어도 1회 반복하여 증폭시키고 ; 각각의 상이한 서열로부터 상기의 증폭된 연장 생성물을 동정하는 단계를 포함함을 특징으로 하는, 다수의 DNA서열에서의 결실을 동시에 탐지하는 방법.
  2. 제1항에 있어서, 언급된 서열이 X 및 Y염색체상의 서열의 그룹으로부터 선택되는, 다수의 게놈성 DNA서열에서의 결실을 탐지하는 방법.
  3. 제2항에 있어서, 게놈성 DNA서열이 유전병을 일으키는 결실을 포함하는 X-관련 유전병을 탐지하는 방법.
  4. 제3항에 있어서, 오르니틴 트랜스카바밀라제 결핍증, 하이포크산틴 포스포리보실트랜스퍼라제 결핍중, 스테로이드 설페이타제 결핍증 및 X-관련 근위축증으로 구성된 그룹중에서 선택되는 X-관련 유전병을 탐지하는 방법.
  5. 제4항에 있어서, 다수의 쌍을 이룬 프라이머가 디스트로핀(dystrophin)단백질을 암호화하는 유전자내의 상이한 서열에 상보적인, X-관련 근위축증을 탐지하는 방법.
  6. 제5항에 있어서, 다수의 쌍을 이룬 프라이머가
    로 구성된 그룹중에서 선택되는 방법.
  7. 제3항에 있어서, 게놈성 DNA가 태아의 조직으로부터 유래된 방법.
  8. 제1항에 있어서, 다수의 쌍을 이룬 프라이머가
    로 구성된 그룹중에서 선택되는, 다수의 게놈성 DNA서열에서의 결실을 탐지하는 방법.
  9. 하기 서열식의 DNA서열 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  10. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  11. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  12. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  13. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  14. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  15. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  16. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
  17. 하기 서열식의 DNA서열, 및 디스트로핀을 암호화하는 유전자의 센스 및 안티-센스 본쇄에 상보적이며 디스트로핀 유전자의 상기 본쇄에 어닐링시키고 디스트로핀 서열을 증폭시킬 수 있는 그의 단편 및 유도체 :
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019890014542A 1988-10-12 1989-10-11 결실 탐지를 위한 게놈성 dna다중 증폭방법 KR900006520A (ko)

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Families Citing this family (129)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE61148B1 (en) * 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
CA1339731C (en) * 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection
US5192659A (en) * 1989-08-25 1993-03-09 Genetype Ag Intron sequence analysis method for detection of adjacent and remote locus alleles as haplotypes
FR2667079B1 (fr) * 1990-09-26 1993-08-13 Genset Sa Procedes, oligonucleotides amorces et oligonucleotides sondes pour la detection de bacteries pathogenes de la cavite buccale.
IE66125B1 (en) * 1991-01-31 1995-12-13 Zeneca Ltd Detection method for variant nucleotides
US5869252A (en) * 1992-03-31 1999-02-09 Abbott Laboratories Method of multiplex ligase chain reaction
US6100099A (en) 1994-09-06 2000-08-08 Abbott Laboratories Test strip having a diagonal array of capture spots
JPH07505293A (ja) * 1992-03-31 1995-06-15 アボツト・ラボラトリーズ 多重リガーゼ連鎖反応方法
US5633134A (en) * 1992-10-06 1997-05-27 Ig Laboratories, Inc. Method for simultaneously detecting multiple mutations in a DNA sample
US5470723A (en) * 1993-05-05 1995-11-28 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US5422252A (en) * 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
EP0630973A3 (en) * 1993-05-14 1995-04-26 Eastman Kodak Co Diagnostic compositions, elements, methods and kits for amplification and detection tests of two or more DNA's using primers at suitable melting temperatures.
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5928905A (en) * 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
NL9401082A (nl) * 1994-06-28 1996-02-01 Ingeny Bv Werkwijze voor het detecteren van mutaties.
US5550020A (en) * 1994-07-08 1996-08-27 Visible Genetics Inc. Method, reagents and kit for diagnosis and targeted screening for retinoblastoma
US20020055101A1 (en) 1995-09-11 2002-05-09 Michel G. Bergeron Specific and universal probes and amplification primers to rapidly detect and identify common bacterial pathogens and antibiotic resistance genes from clinical specimens for routine diagnosis in microbiology laboratories
JP4484967B2 (ja) * 1995-06-06 2010-06-16 ベス イスラエル ディーコネス メディカル センター Ｄｎａ診断試験の方法及び装置
US6838256B2 (en) 1996-02-12 2005-01-04 Gene Logic Inc. Coding sequences of the human BRCA1 gene
US20030022184A1 (en) * 1996-02-12 2003-01-30 Oncormed. Inc. Coding sequences of the human BRCA1 gene
JP3898228B2 (ja) * 1996-04-12 2007-03-28 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク 検出プローブ、キット及びアッセイ
US5811269A (en) * 1996-04-30 1998-09-22 Becton, Dickinson And Company Detection of mycobacteria by multiplex nucleic acid amplification
US6287763B1 (en) * 1996-06-10 2001-09-11 Millennium Pharmaceuticals, Inc. Screening methods for compounds useful in the regulation of body weight
US6124092A (en) 1996-10-04 2000-09-26 The Perkin-Elmer Corporation Multiplex polynucleotide capture methods and compositions
US20050202499A1 (en) 1996-10-31 2005-09-15 Billing-Medel Patricia A. Reagents and methods useful for detecting diseases of the breast
US20050153373A1 (en) * 1996-10-31 2005-07-14 Billing-Medel Patricia A. Reagents and methods useful for detecting diseases of the breast
US20030165971A1 (en) * 1996-10-31 2003-09-04 Billing-Medel Patricia A. Reagents and methods useful for detecting diseases of the breast
US20100267012A1 (en) 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
US20030049636A1 (en) 1999-05-03 2003-03-13 Bergeron Michel G. Species-specific, genus-specific and universal DNA probes and amplification primers to rapidly detect and identify common bacterial and fungal pathogens and associated antibiotic resistance genes from clinical specimens for diagnosis in microbiology laboratories
AU6024598A (en) * 1997-01-10 1998-08-03 Pioneer Hi-Bred International, Inc. Hybridization-based genetic amplification and analysis
US6048689A (en) * 1997-03-28 2000-04-11 Gene Logic, Inc. Method for identifying variations in polynucleotide sequences
US20020137904A1 (en) * 1998-03-27 2002-09-26 Patricia A. Billing-Medel Reagents and methods useful for detecting diseases of the gastrointestinal tract
WO1998048052A2 (en) * 1997-04-18 1998-10-29 Abbott Laboratories Amplification based mutation detection
US20050208567A1 (en) * 1997-04-25 2005-09-22 Billing-Medel Patricia A Reagents and methods useful for detecting diseases of the prostate
US20090269814A1 (en) * 1998-05-22 2009-10-29 Murphy Patricia D Method of Analyzing a BRCA2 Gene in a Human Subject
US6492109B1 (en) * 1997-09-23 2002-12-10 Gene Logic, Inc. Susceptibility mutation 6495delGC of BRCA2
FR2775002B1 (fr) * 1998-02-19 2003-01-10 France Etat Procede de diagnostic d'agents pathogenes respiratoires par biologie moleculaire
US7090975B2 (en) 1998-03-13 2006-08-15 Promega Corporation Pyrophosphorolysis and incorporation of nucleotide method for nucleic acid detection
US6686163B2 (en) 1998-05-06 2004-02-03 Gene Logic Inc. Coding sequence haplotype of the human BRCA1 gene
US6037130A (en) * 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (en) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Pre-selection and isolation of single nucleotide polymorphisms
US6197510B1 (en) 1998-10-01 2001-03-06 Bio-Id Diagnostic Inc. Multi-loci genomic analysis
ATE456677T1 (de) * 1999-07-09 2010-02-15 Gen Probe Inc Hiv-1 detektion mittels nukleinsäureamplifizierung
EP1246935B1 (en) 1999-09-28 2013-08-14 Geneohm Sciences Canada Inc. Highly conserved genes and their use to generate probes and primers for detection of microorganisms
EP1233976A4 (en) * 1999-11-17 2003-06-11 Jiuping Ji SIMULTANEOUS DETECTION OF HBV, HCV AND HIV IN PLASMA SAMPLES WITH THE AID OF A MULTIPLEX FIXING TEST
US7250252B2 (en) * 1999-12-30 2007-07-31 David Aaron Katz Amplification based polymorphism detection
AU2001263226A1 (en) * 2000-05-17 2001-11-26 The Trustees Of Boston University Novel compositions and methods for carrying out multiple pcr reactions on a single sample
EP2351853A1 (en) 2000-06-06 2011-08-03 Life Technologies Corporation Method and devices for multiplexing amplification reactions
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US7087414B2 (en) 2000-06-06 2006-08-08 Applera Corporation Methods and devices for multiplexing amplification reactions
US6727355B2 (en) * 2000-08-25 2004-04-27 Jcr Pharmaceuticals Co., Ltd. Pharmaceutical composition for treatment of Duchenne muscular dystrophy
JP4836366B2 (ja) * 2000-08-25 2011-12-14 雅文 松尾 デュシェンヌ型筋ジストロフィー治療剤
US6582920B2 (en) * 2000-09-01 2003-06-24 Gen-Probe Incorporated Amplification of HIV-1 RT sequences for detection of sequences associated with drug-resistance mutations
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
US20030082605A1 (en) * 2000-09-06 2003-05-01 Hodge Timothy A. Genomic DNA detection method and system thereof
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
US20030087286A1 (en) * 2000-09-06 2003-05-08 Hodge Timothy A. Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles
US7011943B2 (en) * 2000-09-06 2006-03-14 Transnetyx, Inc. Method for detecting a designated genetic sequence in murine genomic DNA
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
EP1358345B1 (en) * 2001-02-07 2009-10-21 Krzysztof Kucharczyk Multitemperature single strand conformation polymorphism (msscp)
WO2002070751A1 (en) * 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
US6428964B1 (en) 2001-03-15 2002-08-06 Exact Sciences Corporation Method for alteration detection
US20040126760A1 (en) * 2001-05-17 2004-07-01 Natalia Broude Novel compositions and methods for carrying out multple pcr reactions on a single sample
US20060014186A1 (en) * 2001-09-04 2006-01-19 Hodge Timothy A Methods for genotype screening of a strain disposed on an adsorbent carrier
DE60231543D1 (de) * 2001-11-19 2009-04-23 Parallele Bioscience Inc Multiplex-oligonukleotidaddition und zielamplifikation
US20110151438A9 (en) 2001-11-19 2011-06-23 Affymetrix, Inc. Methods of Analysis of Methylation
KR100446621B1 (ko) * 2001-12-18 2004-09-07 삼성전자주식회사 다중 중합효소 연쇄반응에 의한 ｍｏｄｙ３유전자의 증폭을 위한 프라이머 세트
US6977162B2 (en) 2002-03-01 2005-12-20 Ravgen, Inc. Rapid analysis of variations in a genome
MXPA04008477A (es) * 2002-03-01 2005-10-26 Ravgen Inc Metodos para deteccion de trastornos geneticos.
US7727720B2 (en) 2002-05-08 2010-06-01 Ravgen, Inc. Methods for detection of genetic disorders
US7442506B2 (en) 2002-05-08 2008-10-28 Ravgen, Inc. Methods for detection of genetic disorders
US9388459B2 (en) 2002-06-17 2016-07-12 Affymetrix, Inc. Methods for genotyping
US7108976B2 (en) * 2002-06-17 2006-09-19 Affymetrix, Inc. Complexity management of genomic DNA by locus specific amplification
KR100442832B1 (ko) * 2002-07-10 2004-08-02 삼성전자주식회사 다중 중합효소 연쇄반응에 의한 ｍｏｄｙ２ 유전자의증폭을 위한 프라이머 세트
US8304184B2 (en) * 2002-07-30 2012-11-06 Baback Gharizadeh Genotyping using multiple variant-specific primer pools
US20050202436A1 (en) * 2002-07-30 2005-09-15 Baback Gharizadeh Target-specific multiple sequencing primer pool for microbial typing and sequencing applications in DNA-sequencing technologies
US7459273B2 (en) * 2002-10-04 2008-12-02 Affymetrix, Inc. Methods for genotyping selected polymorphism
CA2410795A1 (en) * 2002-11-01 2004-05-01 University Of Ottawa A method for the amplification of multiple genetic targets
JP2006508662A (ja) 2002-12-04 2006-03-16 アプレラ コーポレイション ポリヌクレオチドの多重増幅
DE602004030114D1 (de) * 2003-05-20 2010-12-30 Investigen Inc System zum nachweis von polynukleotiden
WO2005003393A2 (en) * 2003-07-02 2005-01-13 Keygene N.V. Splice site aflp
US8114978B2 (en) * 2003-08-05 2012-02-14 Affymetrix, Inc. Methods for genotyping selected polymorphism
JP4476050B2 (ja) * 2004-06-30 2010-06-09 株式会社ニデック 視野計
KR20070085275A (ko) * 2004-09-20 2007-08-27 유니버시티 오브 피츠버그 오브 더 커먼웰쓰 시스템 오브 하이어 에듀케이션 멀티플 모드에 의한 멀티플렉스 반응 억제 방법
US7405044B2 (en) * 2004-10-08 2008-07-29 Reliagene Technologies Inc. Multiplex PCR for simultaneous quantitation of human nuclear, mitochondrial, and male Y-chromosome DNA
US8067175B2 (en) 2005-02-11 2011-11-29 Memorial Sloan-Kettering Cancer Center Methods and compositions for detecting a drug resistant EGFR mutant
US7452671B2 (en) * 2005-04-29 2008-11-18 Affymetrix, Inc. Methods for genotyping with selective adaptor ligation
US8785130B2 (en) * 2005-07-07 2014-07-22 Bio-Id Diagnostic Inc. Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material
US20070054301A1 (en) * 2005-09-06 2007-03-08 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US11306351B2 (en) 2005-12-21 2022-04-19 Affymetrix, Inc. Methods for genotyping
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
WO2007100711A2 (en) * 2006-02-24 2007-09-07 Investigen, Inc. Methods and compositions for detecting polynucleotides
WO2007106579A2 (en) 2006-03-15 2007-09-20 Micronics, Inc. Integrated nucleic acid assays
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US9150906B2 (en) * 2006-06-28 2015-10-06 Bio-Id Diagnostic Inc. Determination of variants produced upon replication or transcription of nucleic acid sequences
WO2008004691A1 (fr) * 2006-07-04 2008-01-10 Shimadzu Corporation appareil pour concevoir des amorces d'amplification d'acides nucléiques, programme pour concevoir des amorces et appareil de serveur pour concevoir des amorces
WO2008147382A1 (en) * 2006-09-27 2008-12-04 Micronics, Inc. Integrated microfluidic assay devices and methods
US20080096193A1 (en) * 2006-10-24 2008-04-24 Charles Robert Bupp Methods and compositions for detecting polynucleotides
US8153372B2 (en) * 2006-12-19 2012-04-10 The Board Of Regents For Oklahoma State University Method for simultaneously determining in a single multiplex reaction gender of donors and quantities of genomic DNA and ratios thereof, presence and extent of DNA degradation, and PCR inhibition within a human DNA sample
US8198027B2 (en) 2006-12-21 2012-06-12 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US7862999B2 (en) * 2007-01-17 2011-01-04 Affymetrix, Inc. Multiplex targeted amplification using flap nuclease
US7771947B2 (en) * 2007-02-23 2010-08-10 Investigen, Inc. Methods and compositions for rapid light-activated isolation and detection of analytes
EP2137523A1 (en) 2007-04-04 2009-12-30 Network Biosystems, Inc. Plastic microfluidic separation and detection platforms
US9388457B2 (en) 2007-09-14 2016-07-12 Affymetrix, Inc. Locus specific amplification using array probes
JP5523327B2 (ja) 2007-10-12 2014-06-18 レオニックス，インコーポレイテッド 統合型マイクロ流体デバイスおよび方法
US20090233809A1 (en) * 2008-03-04 2009-09-17 Affymetrix, Inc. Resequencing methods for identification of sequence variants
US9074244B2 (en) * 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
JP2012529908A (ja) 2009-06-15 2012-11-29 ネットバイオ・インコーポレーテッド 法医学的ｄｎａの定量化のための改善された方法
WO2011003020A1 (en) 2009-07-01 2011-01-06 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
AU2010300475A1 (en) 2009-09-30 2012-05-03 The Regents Of The University Of California Cofactors and methods for use for individuals
CN102740976B (zh) 2010-01-29 2016-04-20 精密公司 取样-应答微流体盒
US20130059762A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US20130059738A1 (en) 2011-04-28 2013-03-07 Life Technologies Corporation Methods and compositions for multiplex pcr
US9957558B2 (en) 2011-04-28 2018-05-01 Life Technologies Corporation Methods and compositions for multiplex PCR
CA3106132C (en) 2011-05-12 2023-08-29 Ande Corporation Methods and compositions for rapid multiplex amplification of str loci
EP2744916A4 (en) 2011-07-13 2015-06-17 Primeradx Inc MULTIMODAL METHODS FOR SIMULTANEOUS DETECTION AND QUANTIFICATION OF MULTIPLE NUCLEIC ACIDS IN A SAMPLE
JP6498125B2 (ja) 2012-12-21 2019-04-10 マイクロニクス， インコーポレイテッド 流体回路および関連する製造方法
US20150346097A1 (en) 2012-12-21 2015-12-03 Micronics, Inc. Portable fluorescence detection system and microassay cartridge
JP6935167B2 (ja) 2012-12-21 2021-09-15 ペルキネルマー ヘルス サイエンシーズ， インコーポレイテッド マイクロ流体使用のための低弾性フィルム
WO2014182844A1 (en) 2013-05-07 2014-11-13 Micronics, Inc. Microfluidic devices and methods for performing serum separation and blood cross-matching
AU2014262726B2 (en) 2013-05-07 2019-09-19 Perkinelmer Health Sciences, Inc. Device for preparation and analysis of nucleic acids
CN105189750B (zh) 2013-05-07 2020-07-28 珀金埃尔默健康科学有限公司 使用粘土矿物和碱性溶液制备含核酸样品的方法
BR112016007391A2 (pt) 2013-10-03 2017-08-01 Oklahoma Med Res Found biomarcadores da atividade, da intensidade e da fase aguda da doença lúpus eritematoso sistêmico
AU2018213315A1 (en) 2017-01-26 2019-07-25 Oklahoma Medical Research Foundation Biomarkers for systemic lupus erythematosus disease activity, and intensity and flare
CA3116539C (en) 2018-10-18 2023-10-03 Oklahoma Medical Research Foundation Biomarkers for a systemic lupus erythematosus (sle) disease activity immune index that characterizes disease activity
WO2022144432A1 (en) 2020-12-31 2022-07-07 Ingenierie Et Analyse En Genetique Environnementale Pathogen detection in liquid matrix

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US4965190A (en) * 1986-07-31 1990-10-23 Howard Hughes Medical Institute Methods for the identification of mutations in the human phenylalanine hydroxylase gene using DNA probes
CA1339731C (en) * 1988-10-12 1998-03-17 Charles T. Caskey Multiplex genomic dna amplification for deletion detection

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