KR900004088B1 - Cosmetic compositions - Google Patents

Abstract

Cosmetic compsns. comprise (a) liposome suspended soln. (or aq. soln.) of superoxide dismutase 0.1-50 wt.%, (b) stabilizer 1-20 wt.% and (c) additive bal. wt.%. Mix aq. components (80-90 deg. C) and lipo components (75-85 deg.C) slowly, cool to 38-45 deg. C, add liposome supended soln. (or aq. soln.) of Cu.Znsuperoxide dismutase extd. from cow liver and cool to room temp. to give the cosmetic compsns.

Description

Cosmetic composition

TECHNICAL FIELD This invention relates to a cosmetic composition. Specifically, It is related with the cosmetic composition containing the superoxayido dismutase which is an enzyme.

Superoxide dismutase (“SOD”) is an enzyme that exists in aerobic tissues and organs of a living body, and specifically refers to O ₂ − (super-oxide) among free radicals in the living body. It is an enzyme that consumes and serves as a catalyst for the next reaction.

O ₂ ^_ + O ₂ ^_ + 2H + → H ₂ O ₂ + O ₂

As a technical document related to such SOD, Korean Patent Publication No. 87-11244 (Application No. 87-4794, Inoue Masayasu et al.) Discloses a method for producing a SOD derivative.

Such SOD is an enzyme component having a pharmacological effect useful for preventing inflammation and ischemic diseases. In the past, SOD has been mainly used for medicaments administered to prevent sequelae after surgery such as heart disease.

In general, such SOD is known to be extracted from bovine red blood cells or from E. coli, and recently, a method for extracting SOD from marine bacteria has also been developed.

The present inventors have been studying on the fact that using the effect of SOD as a component when forming cosmetics in the cosmetic field, not in the pharmaceutical field, will show desirable effects such as preventing skin aging. When it is used in the composition, the original effect is lost to the extent that can not be expected at all, it was found that it can not be easily used in cosmetic composition.

However, after the endless research to stabilize the unstable SOD in the appropriate conditions and used in the cosmetic composition, it was found that the present invention can produce a cosmetic composition of the desired composition while showing the efficacy of the original SOD to complete the present invention.

Accordingly, an object of the present invention is to provide a cosmetic composition having a new composition containing SOD, which is useful as a skin cosmetic or hair cosmetic.

Hereinafter, the present invention will be described in detail.

In the cosmetic composition, the liposome suspension or aqueous solution of Cu.Zn-SOD is contained in 0.1 to 50% by weight of the entire cosmetic composition, while the stabilizer is contained in 1 to 20% by weight, and the remaining amount is other known additives. It is characterized by a cosmetic composition consisting of.

Here, as the stabilizer, for example, one or a mixture of two or more selected from glycerin, sorbitol, polyethylene glycol, ethanol, 1, 3-butylene glycol, propylene glycol and nuylene glycol may be used.

Referring to the present invention in more detail as follows.

First, looking at the preparation method of the SOD-containing cosmetic composition according to the present invention, a mixture of 0.1 to 50% by weight of liposome suspension or aqueous solution of Cu.Zn-SOD or 1 to 20% by weight of stabilizer, and other known additives, Among the stabilizers and other known additives, the aqueous phase component is heated and mixed at 80-90 ° C., the oil phase component is heated and mixed at 75-85 ° C., these are mixed again, and the mixture is slowly cooled to 38-48 ° C., When the liposome suspension or aqueous solution of Cu.Zn-SOD is added to the mixture slowly and cooled to room temperature, a cosmetic composition containing SOD that maintains the original efficacy of SOD can be prepared.

In particular, the present invention relates to a cosmetic composition for skin and hair containing a liposome suspension or aqueous solution of Cu · Zn-SOD made by encapsulating copper and zinc-superoxide dismutase (CuZn-SOD) extracted from bovine liver into liposomes. , The SOD used in the present invention was extracted from the liver of a cow by a conventional method. For the extraction, first, a protein having a large molecular weight was separated and removed, and the SOD-containing extract was purified using a multistage drop technique. That is, purification was performed by primary ion exchange chromatography, secondary ion exchange chromatography, and gel filtration. SOD purified by the above method contains copper zinc in its active site. Is an enzyme.

The activity measurement of the enzyme SOD extracted and purified was measured by the epinephrine method. That is, 0.3 ml of Epinephrine solution at pH 2.0, 3 ml of sodium carbonate buffer at pH 10.2 containing ethylenediaminetetraacetic acid, and 0.3 ml of SOD solution were added to each solution to give a final concentration of 3 × 10 ⁻⁴ mol epinephrine, 1 The reaction was carried out in a 25 ° C. cuvette (CUVETTE) for 3 minutes while measuring the thorax intensity at 480 nm as a spectrophotometer with 10 × ⁴ mol ethylenediaminetetraacetic acid and 0.05 mol buffer.

Here, the control group used the SOD solution treated for 5 minutes at 100 ℃ instead of the SOD solution, the reaction mechanism at this time is the substrate of this SOD due to the presence of O ₂ ^- SOD generated during the oxidation of epinephrine It is based on inhibiting the automatic oxidation of epinephrine to adrenochrome (which absorbs 480 nm of light). At this time, the unit (UNIT) was defined as the amount of enzyme that inhibits 50% of the absorbance value of the control group after the reaction for 3 minutes, and the purity of the purified enzyme was recognized to be almost purified when a single band appeared using disc gel electrophoresis. .

In addition, the following test solution is used to detect the presence or absence of copper in the SOD.

SOD copper-containing test solution

Test solution (1): 0.2 mol of L-cysteine hydrochloric acid solution

Test solution (2): 0.1% of 2.2'-biquinoline solution

That is, 100 ml of the test solution (1) and 300 ml of the test solution (2) were added to 300 ml of the SOD solution, and when shaken appropriately, a pink color with red color was added. It is determined as Cu · Zn-SOD.

The molecular weight of Cu.Zn-SOD used in the present invention was determined by gel filtration using a known standard protein lysozyme, Ovalbumin, alpha-chymotrypsinogen, albumin, etc. to prepare a standard curve and to measure from the standard curve. The molecular weight of the Cu.Zn-SOD was found to be about 31,000 to 33,000.

The pH stability of the SOD enzyme was measured in accordance with the above-described experimental method after adjusting the purified enzyme solution at various pHs for 48 hours, and the pH was stable in the range of 5.5 to 7.8. In addition, the temperature stability of the SOD enzyme was adjusted to pH 7.0 and heat-treated at each temperature for 20 minutes, and after measuring the residual activity was stable up to 55 ℃.

In the composition of the present invention, both SOD aqueous solution and SOD liposome suspension can be used, but in order to maximize the stability of the SOD enzyme, liposomes can be liposomes in multiple layers. Here's how.

Soy phospholipid, phosphatidylcholine, is made into a film (FILM), and superoxide dismutase solution is added thereto and then suspended. The suspension is vortexed for 10 minutes and the dispersion is allowed to equilibrate for 2 hours at room temperature.

The multi-layer liposomes containing SOD are then obtained by centrifuging the dispersion above at 13,500 rpm for 15 minutes in a SORVALL RC 2B freeze centrifuge to separate the unencapsulated enzyme. The liposomes containing SOD are separated, washed with 8 mM calcium chloride solution, the supernatant is discarded, and the remaining concentrated portion is resuspended in 8 mM calcium chloride solution to obtain the final SOD liposome material.

How to determine whether liposomes are formed can be seen by a phase contrast microscope. In order to investigate the efficiency of liposomes containing SOD, 0.025 ml of SOD liposomes on the forearm are treated in advance for 1 to 2 minutes to absorb into the skin. Liposomes without SOD encapsulation and pure SOD solutions are used as controls. Along with the liposomes encapsulated in SOD, 0.05 ml of sodium lauryl sulfate, an inflammation-inducing agent, is applied to the cloth and securely taped to the previously treated area. In this condition, remove and observe the cloth after 2 to 4 hours and immediately record the degree of erythema. At this time, lauric acid may be used as an inflammatory agent.

On the other hand, according to the present invention, both SOD aqueous solution and SOD-encapsulated liposomes and septic products containing them have alleviating inflammation and preventing irritation of the skin. That is, since the SOD aqueous solution in terms of efficacy and the SOD liposome in terms of stability are more preferably used, both the SOD aqueous solution and the liposome suspension in which the SOD is encapsulated can be used in the present invention.

In addition, SOD is a metal-containing protein having a redox metal in its active site, and is separated into three types according to the type of metals contained therein, and each has a unique color. That is, CuZn-SOD, Mn-SOD, Fe-SOD, in particular Cu.Zn-SOD, have a turquoise color, and are mainly distributed in the cytoplasm of eukaryotic cells, human brain and liver, bovine, horse, rat, etc. Mn-SOD and Fe-SOD are widely distributed in pronuclear cells.

On the other hand, free radicals such as SUPEROXIDE ANION are biochemical metabolites that are produced during cellular metabolism by the action of oxygen and are also unstable as they are produced by extracellular physical and chemical environmental factors. Reacts easily with other substances, especially unsaturated fatty acids. However, since most of the cell membranes are composed of fatty acids and lipids, when the free radicals are attacked, the cell membranes are easily destroyed and infiltrated, resulting in impairment of cell metabolism, as well as lipids and oxidation of the cell membranes in vivo. Lipid peroxide is produced with a toxic effect. These free radicals also multiply themselves. In other words, when a free radical interacts with a molecule, it creates a new substance that can cause injury.

From this point of view, O ₂ ^_ is not a powerful damage in itself, but it is directly toxic as it is transformed into a more powerful oxidizer, hydroxyl (OH). This hydroxyl group (.OH) is only generated when hydrogen peroxide is present.

However, SOD can reduce the possibility of hydroxyl formation by eliminating O ₂ ^_ , and can reduce the damage caused by free radicals resulting from tissue damage or inflammation, irritation, acne, blemishes, freckles, and aging that occur during the process. have. Therefore, SOD can be used in cosmetics.

However, such SOD has the above advantages in terms of efficacy, but also has a problem in that it is unstable and easily loses its efficacy as described above. Therefore, the present invention maintains the efficacy of the SOD by properly using a stabilizer, etc., thereby preventing the degeneration of collagen, which is a collagen component in the skin tissue, and the destruction of moisturizers such as hyaluronic acid (HYALURONICACID), thereby improving the skin's moisturizing ability. It is intended to be used.

On the other hand, the progress of skin aging by free radicals, ie, free radicals, leads to lipid peroxidation of cell membranes, resulting in tissue damage, vitamin destruction, and deactivation of other bioenzymes due to its toxicity. In addition, the SOD also prevents the above phenomenon by removing the free group O ₂ ^_ .

In addition, since damage or stimulation to cells is caused by a group derived from O ₂ ^_ or O ₂ ^_ (RADICAL), it is possible to protect normal cellular tissues by using SOD. In other words, O ₂ ^_ can harm biological membranes, either directly or indirectly, so that if its formation is not properly balanced by enzymatic degradation, it can cause an inflammatory reaction, thereby reducing or preventing skin inflammation. You know that you can use.

As described above, according to the present invention, SOD reduces harmful effects on the skin of lauric acid and acne-producing substance SQUALENE, so that SOD can be used as a preventive agent for acne pylori. In addition, SOD may be added to hair products in order to prevent frequent use of hair-containing products containing surfactants, irritation of hair and scalp by perm or dye, or destruction of collagen and hyaluronic acid, which are collagen fibers in the scalp tissue.

As described above, according to the present invention, the SOD may be used in cosmetic products for cosmetics and hair cosmetics, and in order to achieve the above-mentioned efficacy, the SOD is used in an amount of 0.1 to 50% by weight, preferably 0.1 to 100% by weight of the injectable composition. Weight percent to 20 weight percent can be used. If at this time, the use of less than 0.1% by weight is difficult to expect the effect of the added use, if more than 50% by weight is used as a cosmetic composition is impossible.

In addition, the SOD used in the composition of the present invention can use various SODs as described above, but it is particularly preferable to use Cu · Zn-SOD extracted from bovine liver, and a liposome of SOD aqueous solution, SOD suspension, and SOD. Both may be used, but in terms of efficacy, it is preferable to use SOD aqueous solution, and stability in terms of liposomes.

In preparing the cosmetic composition according to the present invention by using such SOD enzyme, in particular, a mixture of liposome suspension or aqueous solution of Cu.Zn-SOD, an aqueous solution, a stabilizer, and other known additives generally used in cosmetic composition is used. First of all, the aqueous phase and the oil phase components of the components except for SOD must be separately heated to form a heterogeneity so that each component can be mixed more preferably. In this way, after mixing the respective components, these mixed components are cooled to 38-45 ° C, where sudden cooling causes unevenness in cosmetic composition, which is undesirable.

After the cooling, the SOD is added thereto. If the temperature is 38 ° C. or lower, the SOD is poorly kneaded with other components, and the uniformity thereof becomes very uneven, resulting in poor efficacy and usability. In addition, if the temperature is more than 45 ℃ SOD is also not preferred because of the loss of activity as an enzyme due to excessive high temperature.

The cosmetic composition thus prepared is finally cooled to room temperature and can be used as a cosmetic as in the known cosmetic composition.

On the other hand, the form of the cosmetic composition applied to the skin according to the present invention is most cosmetic formulations, such as lotion and milk, cream, gel. Specifically, face cream or lotion, massage cream, skin lotion, milk crochet, nourishing cream, pack, essence, base cream, foundation cream, body lotion, sunscreen cream or lotion, whitening cream, whitening lotion , Acne cream or lotion.

The cosmetic composition for skin of the present invention contains a surfactant, a moisturizer, a pigment, a fragrance, a preservative, water, a natural oil, a synthetic oil, a wax, a resin, etc., including SOD. Among the components used in the oil phase are stearic acid, mono-stearic acid glycerin, liquid paraffin, myristic acid isopropyl, squalane, beeswax, ceresin, microcrystalline lead, aliphatic alcohols such as lanolin, petrolatum and cetanol, and meals such as avocado oil. Induction is included. The components used in the water phase are water-soluble active ingredients such as furophilene glycol, sorbitol liquid, glycerin, 1.3-butylene glycol, polyoxyethylene methyl glucoside, ethanol, hyaluronic acid and collagen.

In addition, the cosmetic composition for hair is a surfactant, a cleaning agent, a pigment, a preservative, a hair restorer, a metal ion blocking agent, a thickener, etc., including SOD. The main raw materials used in hair products are detergents such as sodium lauryl sulfate, lauryl triethanolamine, lauric acid diethanolamide, tocopherol, menthol, protein, ethanol, sodium ethylenediaminetetraacetate, paraoxybenzoate, Pigments, including dazolidinyl urea and the like, are also included.

The form of the hair product according to the present invention is included in hair shampoo, hair rinse, hair treatment cream, hair setting gel, hair styling mousse, hair spray, hair tonic, hair liquid and the like.

The composition of the present invention exhibits the effects of skin aging prevention, skin irritation prevention, inflammation prevention and alleviation, acne, blemishes, freckles prevention and inhibition, lipid peroxidation of cell membrane, anti-irritant hair protection, etc.

Hereinafter, in order to explain the present invention in more detail, the following is an example of the effect test and the cosmetic composition.

[Test Example 1]

SOD prevention and mitigation effect test

The test was conducted on 10 healthy male volunteers. Cu-Zn-SOD (20,000 UNITS / ml) solution from bovine liver was sampled. The process is as follows.

First, 0,1,5,10 part aqueous solution of Cu * Zn-SOD was created. Next, a 60% aqueous solution of sodium lauryl sulfate (soaked as SLS under sodium) was prepared as an irritant. Then, a mixture A, B, C, and D were prepared by mixing each of 0,1,5,10 parts aqueous solution of Cu.Zn-SOD with an aqueous SLS solution at 50:50. A part of the subject's back was washed with 70 parts of isopropyl alcohol solution, and mixtures A, B, C, and D were applied to this part. Application of the mixture was carried out using a patching method, and the amount applied was 0.2 gram each. After one day, the skin of the test site was observed. The results are shown in Table 1 below.

TABLE 1

-Is negative, +? Is suspicious positive, + is weak erythema, ++ is pronounced erythema, +++ is swelling and severe erythema with blisters.

As can be seen in Table 1 above, it can be seen that in the mixture containing SOD, the erythema positive reaction is significantly reduced. That is, SOD prevents and alleviates irritation to skin erythema.

[Test Example 2]

Anti-inflammatory and Acne Inhibitory Effect Test of SOD

Cosmetic creams A and B containing 0 parts and 5 parts of the same Cu · Zn-SOD solution as in Example 1 were prepared. The makeup cream composition at this time excludes fragrance or other additives as a kind of excipient. Ten males and ten females with severe acne vulgaris were selected for the forehead. The test process is as follows.

Thirty minutes after the subject washes, cream A, which contains only excipients, was applied to the right side of the forehead, and cream B containing 5 parts of SOD was applied to the left side of the forehead. This treatment was continued twice a day for one week. Observation results for the state after one week is shown in Table 2 below.

[Table 2]

In Table 2, it can be seen that the number of acne on the left forehead using SOD-containing makeup cream B is significantly reduced, compared to SOD-free window cream A, and it can confirm the SOD relief and acne suppression effect.

Example 11

A superoxide dismutase aqueous solution is added to the soybean phospholipid (Phosphatidylcholine), suspended, the suspension is vortexed for 10 minutes and the dispersion is allowed to equilibrate at room temperature for two hours.

Then sorbbal it. Multiple layers of liposomes containing SOD are obtained by separating unencapsulated enzyme by centrifugation at 13,500 rpm for 15 minutes in a RC 2B frozen centrifuge. The liposomes containing the separated SOD are washed with 8 mM calcium chloride solution, the supernatant is removed and the remaining part is resuspended in 8 mM calcium chloride solution. Thus, the final liposomes can be obtained. Check with a phase contrast microscope to see if liposomes have formed.

Using a liposome suspension of Cu Zn-SOD treated as described above, the composition was prepared according to the following formulation to obtain a SOD-containing moisture lotion.

1.SOD liposome suspension 2 parts

2 . Stearic acid, part 5

3. Cetanol3

4. Part 10 of liquid paraffin

5. 1.5 parts wax

6.myristin isopropyl 3.7part

7. Butyl paraoxybenzoate 0.2 part

8. Monostearic acid sorbitan part 2

9. Spices 0.4 part

10. Propylene glycol 6 parts

11.paraoxybenzoic acid methyl0.2part

12.66 purified water

The raw material 2-9 is dissolved by heating to 80 ° C., added to 10-12 heated to 85 ° C., emulsified, cooled to 40 ° C., and then the raw material 1 is added, stirred, and cooled to room temperature.

Example 2

Cleansing cream containing SOD liposomed in the same manner as in Example 1.

1.SOD liposome suspension 1 part

2. 4 copies of beeswax

3. Ceresin 2.3

4. Paraffin 1.5 parts

5. Dibutylhydroxy toluene 0.02 parts

6. Butyl paraoxybenzoate 0.2 part

7. Sesquioleic acid sorbitan part 2

8. Liquid paraffin 50 parts

9. 0.5 parts fragrance

10.glycerine 3.5 parts

11.0.1 part of paraoxybenzoic acid methyl

12.imidazolidinyl urea0.1part

13. Pigmentation

14.34.78 parts of purified water

The raw material 10-14 is dissolved by heating to 85 ° C., then heated to 80 ° C., added to the dissolved 2-9, emulsified, cooled to 40 ° C., then the raw material 1 is added, stirred, and cooled to room temperature.

Example 3

Massage cream containing SOD liposomeized in the same manner as in Example 1.

0.5 part SOD liposome suspension

2. Vaseline Part 5

3. Ceresin Part 3

4 . Lanolin 2.5

5. Cetanol1.8part

6. 3.5 wax

7. Liquid paraffin 43

8. Polyoxyethylene stearyl ether 2 parts

9. 0.2 parts of butyl paraoxybenzoates

10.Fragrance 0.3 parts

11.Sorbitol solution 4 parts

12.imidazolidinylurea0.2part

13. Polyethylene glycol 1500 part 1

14. Pigmentation

15. 33 parts of purified water

The raw materials 11-15 are heated to 85 ° C. to be dissolved, added to 2-10 dissolved by heating to 80 ° C., emulsified, cooled to 40 ° C., and then the raw material 1 is added, stirred, and cooled to room temperature.

Example 4

A skin astringent containing SOD liposomes in the same manner as in Example 1.

1.5 parts of SOD liposome suspension

2. 10 parts ethanol

3. Paraoxybenzoic acid methyl0.1part

4. 1 part polyoxyethylene nonyl phenyl ether

5. Perfume 0.2 part

6. Citric acid 0.2 part

7.Sodium Citrate0.6part

8.Benzophenone-90.05part

9. Witch Hazel Extract Part 1

10.imidazolidinyl urea0.1part

11.pigmentation

12. 1, 3-butylene glycol 7 parts

13.78.25 parts of purified water

1-5 which was dissolved at room temperature and dissolved was added to 6-13, which was stirred and dissolved at room temperature, and uniformly mixed.

Example 5

Narisin cream containing SOD liposomes in the same manner as in Example 1.

1.2 parts of SOD liposome suspension

2 . Stearic acid part 3

3. Cetanol 2 parts

4 . 1.5 microcrystalline lead

5. lipophilic monostearate glycerine 5 parts

6. Liquid lanolin 4 parts

7. 15 Octyl Dodecane

8 . 3.5 parts isopropyl palmitate

9. Squalane Part 5

10. Myri Steel Pre-Part 2.3

11.Polyoxyethylene Cetyl Ether 1.8

12.Methyl polysiloxane0.5part

13.0.35 flavors

14. Polyoxyethylene methyl glucoceido part 2

15.glycerine 3 parts

16.Sorbitol part 5

17. paraoxybenzoic acid methyl 0.2 part

18. 44.65 parts of purified water

The raw material 14-18 is heated to 85 ° C. to be dissolved, added to 2-13 dissolved by heating to 80 ° C., emulsified, cooled to 40 ° C., and then the raw material 1 is added, stirred, and cooled to room temperature.

Example 6

Facial pack containing SOD liposomes in the same manner as in Example 1.

1.3 parts of SOD liposome suspension

2 . 0.5 parts of aminocaproic acid

3. Benzophenone-90.1part

4 . Carboxymethyl Cellulose Sodium 1 part

5. Polyvinyl pyriridone part 2

6. Carboxyvinyl polymer 0.5 parts

7.imidazolidinyl urea0.2part

8. Pigment Proper

9. Propylene glycol 14 parts

10. 70.3 parts of purified water

11.0.1 flavors

12 ethanol 10 parts

The raw materials 2-10 are uniformly dispersed at 80 ° C, 11-11 is added, cooled to 40 ° C, 1 is added and mixed.

Example 7

Nutrient essence containing SOD liposomed in the same manner as in Example 1.

1.SOD liposome suspension 3 parts

2 . Propylene glycol 30 parts

3. Glycerin 10 parts

4. 5 parts of sorbitol solution

5. paraoxybenzoic acid methyl 0.2 part

6. Pigment Proper

7. Higeshishichi cellulose 0.2 part

8. 46.45 parts of purified water

9. 0.15 parts fragrance

10.ethanol5parts

Raw materials 2-8 are heated to 80 ° C. to be uniformly dispersed, and then 9-10 is added, cooled to 40 ° C., 1 is added, and mixed.

Example 8

Bodiroshon containing SOD liposomes in the same manner as in Example 1.

1.SOD liposome suspension 0.7 parts

2 . Stearic acid part 3

3. Stearyl Alcohol Part 2

4. Ole Alcohol 5 parts

5. 2.5 parts of payment

6. Olive oil part 3

7. Myristic acid octyldodecyl part 6

8. 2.7 parts of sorbitan monostearate

9. Spices 0.45 parts

10. Carboxyvinyl Polymer 0.2 part

11.hexylene glycol 2 parts

12.glycerine 3 parts

13. Pigment amount

14.69.45 parts purified water

The raw material 2-9 is heated to 80 ° C. to dissolve it, added to 10-14 heated to 85 ° C., emulsified, cooled to 40 ° C., and then the raw material 1 is added, stirred, and cooled to room temperature.

Example 9

Creamed foundation containing SOD liposomed in the same manner as in Example 1.

0.4 part of SOD liposome suspension

2. Behenic acid part 3

3. Cetanol 2 parts

4 . Lanolin Fatty Acid Isopropyl 2.3parts

5. 1.5 parts of monostearic acid ethylene glycol

6. 1 part self-emulsifying glycerin monostearate

7. 0.3 parts fragrance

8.triethanolamine2part

9. paraoxybenzoic acid methyl0.2part

10. Propylene glycol 8 parts

11.0.5 parts of ferric oxide

12.Black iron oxide 0.1part

13.1 yellow iron oxide

14. Titanium Dioxide Part 5

15.72.7 parts purified water

8-15 heated to 85 ° C. is added to 2-7 heated to 80 ° C., emulsified, cooled to 40 ° C., raw material 1 is added, and stirred to room temperature.

Example 10

Sunscreen cream containing SOD liposomes in the same manner as in Example 1.

0.3 parts of SOD liposome suspension

2 . Shea Butter Part 3

3. Two beeswax

4. 1 part cetanol

5. Olive oil part 5

6. Octyldimethylmethyl part 6

7. Micromethicone Part 2

8. 0.25 parts of spices

9. Titanium Dioxide Part 1

10. 1, 3-butylene glycol 3 parts

11.76.45 parts of purified water

9-11 heated to 85 ° C. was added to 2-8 dissolved in 80 ° C., emulsified, cooled to 40 ° C., then raw material 1 was added, stirred, and cooled to room temperature.

Example 11

Whitening lotion containing SOD aqueous solution.

1.2.3 SOD aqueous solution

2. Ceresin Part 3

3. Shea butter part 1

4 . Lanolin 1.5 part

5. Polyoxyethylene lanolin 2 part

6. Octyl palmitate 10 parts

7. Part 4 Oil

8.butyl paraoxybenzoate 0.15 parts

9. 0.3 parts fragrance

10.ascorbic acid 0.4part

11.glycerol 5 parts

12.70.35 parts of purified water

The raw material 2-9 is dissolved by heating to 80 ° C., added to 10-12 heated to 85 ° C., emulsified, cooled to 40 ° C., and then the raw material 1 is added, stirred, and cooled to room temperature.

Example 12

Akneroshone containing SOD liposomed in the same manner as in Example 1.

1.SOD liposome suspension 3 parts

2. Almond oil part 5

3. Apricot kernel oil part 4

4 . Microcrystalline Lead Part 2

5. Polyoxyethylene Cetyl ether 1 part

6. 1.5 parts sorbitan monostearate

7. 0.1 parts fragrance

8. Glycerin Part 3

9. Allantoin 0.2 part

10. Chamomile Extract 1 copy

11.0.5 parts of collagen

12. Purified water 78.7 parts

The raw material 2-7 is dissolved by heating to 80 ° C., added to 8-12 dissolved in 85 ° C., emulsified, cooled to 40 ° C., and then the raw material 1 is added, stirred, and cooled to room temperature.

Example 13

Hair shampoo containing SOD liposomes in the same manner as in Example 1.

0.6 parts of SOD liposome suspension

2 . 10 parts sodium lauryl sulfate

3. 18 parts of lauryl sulfate triethanolamine

4. Palm oil fatty acid diethanolamide 5 parts

5.Mysteric Isopropyl 0.4part

6.0.2 parts of paraoxybenzoic acid methyl

7. 3 parts propylene glycol

8. Pigment Proper

9. 0.5 parts fragrance

10. 62.3 parts purified water

After dissolving 2-10 by heating it to 80 ° C, cooling it to 40 ° C, adding 1 and mixing, it is regarded as room temperature.

Example 14

Hair tonic with SOD aqueous solution.

1.SOD aqueous solution 0.9 parts

2. L-menthol 0.1 part

3. DL-Kampa 0.2 part

4 . Tocopherol acetate0.5part

5. 1 part aminocaproic acid

6. Resofcin 0.02

7. Propylene glycol 5 parts

8. 0.4 parts of fragrance

9.ethanol 91.88parts

Raw materials 1-9 are mixed by the method according to the invention and finally homogeneously mixed at room temperature.

Claims (3)

In the cosmetic composition, an effective amount of superoxide dismutase is contained, but liposome suspension or aqueous solution of superoxide dismutase is contained in 0.1 to 50% by weight of the entire cosmetic composition, and 1 to 20% by weight of a stabilizer is contained. The remaining amount is a cosmetic composition, characterized in that consisting of other known additives. The cosmetic composition according to claim 1, wherein the superoxide dismutase is a Cu.Zn-superoxido dismutase extracted from bovine liver and has a molecular weight of 31,000 to 33,000. In the preparation of the cosmetic composition, 0.1-50% by weight of a liposome suspension or aqueous solution of Cu.Zn-superoxide dismutase, 1-20% by weight of a stabilizer, and other known additives are mixed. Among other known additives, the water phase component is heated to 80-90 ° C., the oil phase component is heated to 75-85 ° C. and mixed with each other, and the mixture is slowly cooled to 38-45 ° C., and the Cu.Zn- is added thereto. A liposome suspension or aqueous solution of superoxide dismutase is added slowly to mix and cooled to room temperature.