KR890000661A - 담체 결합 효소의 제조방법 - Google Patents

담체 결합 효소의 제조방법 Download PDF

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Publication number
KR890000661A
KR890000661A KR1019880006932A KR880006932A KR890000661A KR 890000661 A KR890000661 A KR 890000661A KR 1019880006932 A KR1019880006932 A KR 1019880006932A KR 880006932 A KR880006932 A KR 880006932A KR 890000661 A KR890000661 A KR 890000661A
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South Korea
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carrier
binding enzyme
producing
carrier binding
polyethylene imine
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KR1019880006932A
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KR970004939B1 (ko
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뮈케 잉거
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비. 마이어, 라우어
칼리-케미 악티엔게젤샤프트
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
    • C12N11/089—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/24—Preparation of compounds containing saccharide radicals produced by the action of an isomerase, e.g. fructose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

내용 없음

Description

담체 결합 요소의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음

Claims (16)

  1. 공유결합을 형성할 수 있는 관능기를 가진 담체를 효소로 피복시킨 담체 결합 효소의 제조방법에 있어서, 그 담체 결합 효소를 이관능기 교차 결합제와 폴리아민으로 임의의 순서로 부가 처리함을 특징으로 하는 담체 결합 효소의 제조방법.
  2. 제1항에 있어서, 담체 결합 효소를 먼저 폴리아민으로 처리한 다음 교차 결합제로 처리함을 특징으로 하는 담체 결합 효소의 제조방법.
  3. 제1항 또는 제2항 중의 어느 한 항에 있어서, 이관능기 교차 결합제로서 글루타릭 디알데히드를 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
  4. 제3항에 있어서, 건조 촉매 물질 1그램당 글루타릭 디알데히드를 5-500mg, 바람직하게는 25-250mg사용하여 처리함을 특징으로 하는 담체 결합 효소의 제조방법.
  5. 제3항 또는 제4항중 어느 한 항에 있어서, 글루타릭 디알데히드를 0.1-10％(W/V) 수용액으로, 바람직하게는 0.5-5％(W/V) 수용액으로서 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
  6. 제3항 또는 제5항중 어느 한 항에 있어서, 글루타릭 디알데히드 처리를 pH 4-9, 바람직하게는 pH 5-8에서 수행함을 특징으로 하는 담체 결합 효소의 제조방법.
  7. 제3항 또는 제6항중 어느 한 항에 있어서, 글루타릭 디알데히드 처리를 15-120분동안, 바람직하게는 30-60분동안 수행함을 특징으로 하는 담체 결합 효소의 제조방법.
  8. 제1항 또는 제2항중 어느 한 항에 있어서, 폴리아민으로서 폴리에틸렌 이민을 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
  9. 제8항에 있어서, 분자량이 2,000,000 달톤이하인 폴리에틸렌 이민을 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
  10. 제9항에 있어서, 분자량이 600-1,000,000 달톤 사이인 폴리에틸렌 이민을 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
  11. 제8항 또는 제10항중 어느 한 항에 있어서, 건조 촉매 물질 1그램당 폴리에틸렌 이민을 3-600mg, 바람직하게는 15-240mg 사용하여 처리함을 특징으로 하는 담체 결합 효소의 제조방법.
  12. 제8항 또는 제11항중 어느 한 항에 있어서, 폴리에틸렌 아민을 0.1-20％(W/V) 수용액으로, 바람직하게는 0.5-8％(W/V) 수용액으로서 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
  13. 제8항 또는 제12항중 어느 한 항에 있어서, 폴리에틸렌 이민 처리를 pH 4-9, 바람직하게는 pH 5-8에서 수행함을 특징으로 하는 담체 결합 효소의 제조방법.
  14. 제8항 또는 제13항중 어느 한 항에 있어서, 폴리에틸렌 이민 처리를 적어도 5분, 바람직하게는 적어도 15분간 수행함을 특징으로 하는 담체 결합 효소의 제조방법.
  15. 전항중 어느 한 항에 있어서, 산화 알루미늄 또는 규산에 기초한 다공질 무기 담체를 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
  16. 전항중 어느 한 항에 있어서, 글루코스 이소머라제를 효소로 사용함을 특징으로 하는 담체 결합 효소의 제조방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019880006932A 1987-06-10 1988-06-09 담체 결합 효소의 제조방법 KR970004939B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DEP3719324.4 1987-06-10
DE3719324A DE3719324C1 (de) 1987-06-10 1987-06-10 Verfahren zur Herstellung traegergebundener Enzyme

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KR890000661A true KR890000661A (ko) 1989-03-16
KR970004939B1 KR970004939B1 (ko) 1997-04-10

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US (1) US4940664A (ko)
EP (1) EP0294711B1 (ko)
JP (1) JPS63317085A (ko)
KR (1) KR970004939B1 (ko)
AT (1) ATE86667T1 (ko)
DE (2) DE3719324C1 (ko)
DK (1) DK175038B1 (ko)
ES (1) ES2053623T3 (ko)
FI (1) FI101400B1 (ko)
HU (1) HU204084B (ko)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
KR20010097881A (ko) * 2000-04-26 2001-11-08 김길용 1자형 고체 비료의 제조방법

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DE4024491A1 (de) * 1990-08-02 1992-02-06 Kali Chemie Ag Enzymtraeger auf anorganischer basis und traegergebundene enzyme
JP3125428B2 (ja) * 1991-03-28 2001-01-15 和光純薬工業株式会社 修飾酵素
US6033502A (en) * 1996-11-13 2000-03-07 Kimberly-Clark Worldwide, Inc. Process and apparatus for registering continuously moving stretchable layers
WO2002070646A2 (en) * 2001-02-16 2002-09-12 Ibex Technologies Matrix stabilized enzyme crystals and methods of use
EP1657298A1 (de) * 2004-11-12 2006-05-17 Cognis IP Management GmbH Feste Zubereitungen
US9303256B2 (en) * 2005-09-30 2016-04-05 Novozymes A/S Immobilization of enzymes
CN104418297B (zh) * 2013-09-11 2017-03-01 中国科学院理化技术研究所 含有高分子聚乙烯亚胺修饰的多羰基二铁二硫簇化合物的光催化产氢体系、制法及制氢方法

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US3796634A (en) * 1970-03-19 1974-03-12 Us Health Education & Welfare Insolubilized biologically active enzymes
US4141857A (en) * 1976-04-30 1979-02-27 Uop Inc. Support matrices for immobilized enzymes
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US4251631A (en) * 1978-02-23 1981-02-17 Research Products Rehovot Ltd. Cross-linked enzyme membrane
US4337172A (en) * 1981-01-15 1982-06-29 Uop Inc. Enhanced immobilization of a glucose isomerase
DE3148603C1 (de) * 1981-12-09 1983-07-21 Kali-Chemie Ag, 3000 Hannover Verfahren und Anlage zur Herstellung von Isomerose
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US4504582A (en) * 1982-07-20 1985-03-12 Genex Corporation Vermiculite as a carrier support for immobilized biological materials
JPS5945883A (ja) * 1982-09-06 1984-03-14 Nitto Electric Ind Co Ltd 固定化酵素及びその製造方法
JPS5945884A (ja) * 1982-09-06 1984-03-14 Nitto Electric Ind Co Ltd 固定化酵素及びその製造方法
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010097881A (ko) * 2000-04-26 2001-11-08 김길용 1자형 고체 비료의 제조방법

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DE3878993D1 (de) 1993-04-15
FI101400B (fi) 1998-06-15
FI882732A0 (fi) 1988-06-09
HUT50211A (en) 1989-12-28
DK175038B1 (da) 2004-05-10
FI882732A (fi) 1988-12-11
JPS63317085A (ja) 1988-12-26
KR970004939B1 (ko) 1997-04-10
HU204084B (en) 1991-11-28
EP0294711B1 (de) 1993-03-10
FI101400B1 (fi) 1998-06-15
ATE86667T1 (de) 1993-03-15
ES2053623T3 (es) 1994-08-01
EP0294711A1 (de) 1988-12-14
DE3719324C1 (de) 1988-12-15
DK313288A (da) 1988-12-11
DK313288D0 (da) 1988-06-09
US4940664A (en) 1990-07-10

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