KR880700064A - 고무 폴리머라제 및 이의 제법과 용도 - Google Patents
고무 폴리머라제 및 이의 제법과 용도Info
- Publication number
- KR880700064A KR880700064A KR860700866A KR860700866A KR880700064A KR 880700064 A KR880700064 A KR 880700064A KR 860700866 A KR860700866 A KR 860700866A KR 860700866 A KR860700866 A KR 860700866A KR 880700064 A KR880700064 A KR 880700064A
- Authority
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- South Korea
- Prior art keywords
- mentioned
- rubber
- polymerase
- latex
- rubber polymerase
- Prior art date
Links
- 229920001971 elastomer Polymers 0.000 title claims 35
- 238000000034 method Methods 0.000 claims 46
- 239000000243 solution Substances 0.000 claims 17
- 239000004816 latex Substances 0.000 claims 15
- 229920000126 latex Polymers 0.000 claims 15
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 12
- 239000011541 reaction mixture Substances 0.000 claims 10
- 244000043261 Hevea brasiliensis Species 0.000 claims 9
- 239000000872 buffer Substances 0.000 claims 8
- 229920003052 natural elastomer Polymers 0.000 claims 8
- 229920001194 natural rubber Polymers 0.000 claims 8
- 238000011534 incubation Methods 0.000 claims 7
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims 6
- 230000000087 stabilizing effect Effects 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- 230000006641 stabilisation Effects 0.000 claims 5
- 238000011105 stabilization Methods 0.000 claims 5
- -1 sulfhydryl compound Chemical class 0.000 claims 5
- 241000196324 Embryophyta Species 0.000 claims 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims 4
- 229920001577 copolymer Polymers 0.000 claims 4
- CBIDRCWHNCKSTO-UHFFFAOYSA-N prenyl diphosphate Chemical compound CC(C)=CCO[P@](O)(=O)OP(O)(O)=O CBIDRCWHNCKSTO-UHFFFAOYSA-N 0.000 claims 4
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims 3
- 241000221020 Hevea Species 0.000 claims 3
- 239000002250 absorbent Substances 0.000 claims 3
- 230000002745 absorbent Effects 0.000 claims 3
- HGLAYHSJJRYIBI-UHFFFAOYSA-N allyl diphosphate Chemical compound OP(O)(=O)OP(O)(=O)OCC=C HGLAYHSJJRYIBI-UHFFFAOYSA-N 0.000 claims 3
- 238000013375 chromatographic separation Methods 0.000 claims 3
- 229920003211 cis-1,4-polyisoprene Polymers 0.000 claims 3
- 150000001875 compounds Chemical class 0.000 claims 3
- 239000003456 ion exchange resin Substances 0.000 claims 3
- 229920003303 ion-exchange polymer Polymers 0.000 claims 3
- NUHSROFQTUXZQQ-UHFFFAOYSA-N isopentenyl diphosphate Chemical compound CC(=C)CCO[P@](O)(=O)OP(O)(O)=O NUHSROFQTUXZQQ-UHFFFAOYSA-N 0.000 claims 3
- 229910021645 metal ion Inorganic materials 0.000 claims 3
- 239000002245 particle Substances 0.000 claims 3
- 229920001184 polypeptide Polymers 0.000 claims 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims 3
- QLBHNVFOQLIYTH-UHFFFAOYSA-L dipotassium;2-[2-[bis(carboxymethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [K+].[K+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O QLBHNVFOQLIYTH-UHFFFAOYSA-L 0.000 claims 2
- 229940058180 edetate dipotassium anhydrous Drugs 0.000 claims 2
- 230000000855 fungicidal effect Effects 0.000 claims 2
- 239000000417 fungicide Substances 0.000 claims 2
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 2
- 238000004255 ion exchange chromatography Methods 0.000 claims 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims 2
- 238000002156 mixing Methods 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- 239000011347 resin Substances 0.000 claims 2
- 229920005989 resin Polymers 0.000 claims 2
- 238000000926 separation method Methods 0.000 claims 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims 2
- PUZPDOWCWNUUKD-UHFFFAOYSA-M sodium fluoride Chemical group [F-].[Na+] PUZPDOWCWNUUKD-UHFFFAOYSA-M 0.000 claims 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims 1
- 108010024636 Glutathione Proteins 0.000 claims 1
- 108010065958 Isopentenyl-diphosphate Delta-isomerase Proteins 0.000 claims 1
- 102000004317 Lyases Human genes 0.000 claims 1
- 108090000856 Lyases Proteins 0.000 claims 1
- 229940122907 Phosphatase inhibitor Drugs 0.000 claims 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 claims 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 claims 1
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical group [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims 1
- 229930006000 Sucrose Natural products 0.000 claims 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 claims 1
- 150000001450 anions Chemical class 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 239000001506 calcium phosphate Substances 0.000 claims 1
- 229910000389 calcium phosphate Inorganic materials 0.000 claims 1
- 235000011010 calcium phosphates Nutrition 0.000 claims 1
- 239000002738 chelating agent Substances 0.000 claims 1
- 239000002131 composite material Substances 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- 235000011180 diphosphates Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000001747 exhibiting effect Effects 0.000 claims 1
- 238000001914 filtration Methods 0.000 claims 1
- 238000001641 gel filtration chromatography Methods 0.000 claims 1
- 229960003180 glutathione Drugs 0.000 claims 1
- 229910001385 heavy metal Inorganic materials 0.000 claims 1
- 239000003112 inhibitor Substances 0.000 claims 1
- 239000012528 membrane Substances 0.000 claims 1
- 239000000178 monomer Substances 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 claims 1
- 235000013024 sodium fluoride Nutrition 0.000 claims 1
- 239000011775 sodium fluoride Substances 0.000 claims 1
- 239000000600 sorbitol Substances 0.000 claims 1
- 239000003381 stabilizer Substances 0.000 claims 1
- 239000005720 sucrose Substances 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F136/00—Homopolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds
- C08F136/02—Homopolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds
- C08F136/04—Homopolymers of compounds having one or more unsaturated aliphatic radicals, at least one having two or more carbon-to-carbon double bonds the radical having only two carbon-to-carbon double bonds conjugated
- C08F136/08—Isoprene
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/098—Esters of polyphosphoric acids or anhydrides
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
- C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
- C07F9/02—Phosphorus compounds
- C07F9/06—Phosphorus compounds without P—C bonds
- C07F9/08—Esters of oxyacids of phosphorus
- C07F9/09—Esters of phosphoric acids
- C07F9/113—Esters of phosphoric acids with unsaturated acyclic alcohols
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08C—TREATMENT OR CHEMICAL MODIFICATION OF RUBBERS
- C08C1/00—Treatment of rubber latex
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
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- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
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- Y10S435/00—Chemistry: molecular biology and microbiology
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Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (55)
- 거의 순수한 형태의 고무 폴리머라제(polymerae).
- 제1항에 있어서, 언급된 폴리머라제가 헤베아(Hevea)속의 식물로부터 회수되는 고무 폴리머라제.
- 제1항에 있어서, 언급된 폴리머라제가 헤베아 브라질린시스(Hevea brasiliensis) 라텍스로부터 회수되는 고무 폴리머라제.
- 제1항에 있어서, 언급된 폴리머라제가 단량체성이며, 나트륨도데실술페이트폴리아크릴아미드 겔전기 영동에 의해 측정된 것으로서 약 36,000 내지 약 44,000의 분자량을 갖는 고무 폴리머라제.
- 고무 폴리머라제 활성을 나타내는 하나이상의 활성부위를 갖는 거의 순수한 폴리펩티드로 이루어진 고무 폴리머라제.
- 제5항에 있어서, 언급된 폴리펩티드가 헤베아브라질린시스 라텍스로부터 회수된 고무폴리머라제와 거의 동종성을 나타내는 고무 폴리머라제.
- 제5항에 있어서, 언급된 하나이상의 활성 부위가 헤베아브라질린시스 라텍스로부터 회수된 고무 폴리머라제의 활성부위에 대해 효소학적으로 상당하게 작요하는 고무 폴리머라제.
- 제5항에 있어서, 언급된 하나이상의 활성부위가 헤베아 브라질린시스 라텍스로부터 회수된 고무 폴리머라제상의 상응하는 부위로부터 변화되어 증간된 고무 폴리머라제합성을 갖는 폴리펩티드를 형성하는 고무 폴리머라제.
- 라텍스중의 고무 입자에 결합된 고무 폴리머라제 및 용액중에 유리된 고무 폴리머라제를 함유하는 헤베아 속의 식물로부터 화학적으로 안정화된 라텍스를 제조하고, 라텍스로부터 용액중에 유리된 고무 폴리머라제를 분리시킨 다음, 분리된 고무 폴리머라제를 정제하여 거의 순수한 형태의 고무 폴리머라제를 수득함을 특징으로하여, 거의 순수한 형태의 고무 폴리머라제를 제조하는 방법.
- 제9항에 있어서, 언급된 식물이 헤베아 브라질린시스인 방법.
- 제9항에 있어서, 언급된 안정화된 라텍스가, 폴리하이드록시 화합물, 살균제, 및 완충제로 이루어진 안정화 용액 약 1 내지 약 10부피를 언급된 라텍스 약 1부피에 첨가하는 방법에 의해 제조되는 방법.
- 제11항 있어서, 언급된 안정화 용액이 추가로 술프하이드릴 화합물을 함유하는 방법.
- 제11항에 있어서, 언급된 폴리하이드록시 화합물이 글리세롤, 소르비톨, 및 수크로스 중에서 선택되는 방법.
- 제11항에 있어서, 언급된 안정화 용액이 글리세롤 약 10 내지 약 80중량/부피%, 나트륨 아지드 약 0.001 내지 약 1중량/부피%, 및 완충제로 이루어지며, 언급된 용액이 약 7 내지 약 12의 pH를 갖는 방법.
- 제14항에 있어서, 언급된 안정화 용액이 글리세롤 약 50중량/부피%, 나트륨 아지드 약 0.3중량/부피%, 및 중탄산나트륨 약 0.1M로 이루어지는 방법.
- 제15항에 있어서, 언급된 안정화 용액이 추가로 약 5mM 시스테인을 함유하는 방법.
- 제9항에 있어서, 언급된 분리가 크로마토그라피분리로 이루어지는 방법.
- 제17항에 있어서, 언급된 크로마토그라피 분리가 이온 교환 크로마토그라피로 이루어지는 방법.
- 제18항에 있어서, 이온 교환크로마토그라피를 사용한 크로마토그라피 분리가, 용액중에 유리된 고무폴리머라제가 수지에 결합하여 폴리머라제-수지 복합체를 형성하도록 이온 교환수지를 라텍스에 혼합하고, 복합체를 분리시킨 다음, 복합체로부터 고무폴리머라제를 회수함으로써 이루어지는 방법.
- 제19항에 있어서, 언급된 이온 교환수지가 음이온 이온교환수지인 방법.
- 제9항에 있어서, 언급된 분리가 0 내지 4℃에서 수행되는 방법.
- 제9항에 있어서, 언급된 정제가, 분리된 고무 폴리머라제를 투석시켜 투석물을 생성시키고, 투석물에 겔을 첨가하여 겔폴리머라제 복합체를 형성시킨 다음, 복합체를 투석물로부터 분리시키고, 폴리머라제를 복합체로부터 회수한 다음, 회수된 폴리머라제를 겔여과 크로마토그라피시켜 거의 순수한 형태의 고무 폴리머라제를 생성시킴으로써 이루어지는 방법.
- 제22항에 있어서, 언급된 투석물을 단백질에 대한 흡수제와 혼합하고 투석물에 겔을 첨가하기 전에 흡수제를 제거하는 추가의 단계를 포함하는 방법.
- 제23항에 있어서, 언급된 흡수제가 알루미나 C 감마인 방법.
- 제22항에 있어서, 언급된 겔이 칼슘포스페이트 겔인방법.
- 제9항에 있어서, 언급된 정제가, 용액중에 유리된 고무폴리머라제를 고무 폴리머라제에 대한 부동화된 항체와 접촉시켜, 고무 폴리머라제-항체 복합체를 형성시키고, 용액으로부터 복합체를 분리시킨 다음, 복합체로부터 고무 폴리머라제를 회수하는 단계들로 이루어지는 방법.
- 헤베아속의 식물로부터 화학적으로 안정화된 라텍스를 제조하고, 화학적으로 안정화된 라텍스를 고무 폴리머라제에 대한 부동화된 항체와 잡촉시켜 고무 폴리머라제-항체 복합체를 형성시킨 다음, 라텍스로부터 복합체를 분리시키고, 복합체로부터 고무폴리머라제를 거의 순수한 형태로 회수하는 단계들로 이루어진 거의 순수한 형태의 고무 폴리머라제를 제조하는 방법.
- 제9항의 방법에 의해 제조된 거의 순수한 고무 폴리머라제.
- 폴리하이드록시 화합물, 살균제, 및 완충제로 이루어진 안정화 조성물을 라텍스에 첨가함으로써 라텍스를 안정화시키는 방법.
- 제29항에 있어서, 언급된 안정화 조성물이 술프하이드릴 화합물을 추가로 함유하는 방법.
- 제29항에 있어서, 안정화 조성물이 용액인 방법.
- 제31항에 있어서, 언급된 용액이 약 7내지 약 12의 pH를 갖는 방법.
- 제31항에 있어서, 안정화 용액 약 1 내지 약 10부피를 라텍스 약 1부피에 첨가하는 방법.
- 제31항에 있어서, 안정화 용액 약 2부피를 라텍스 약 1부피에 첨가하는 방법.
- 제31항에 있어서, 언급된 안정화 용액이 글리세롤 약 50중량/부피%, 나트륨 아지드 약 0.3중량/부피%, 및 중탄산나트륨 약 0.1M으로 이루어지는 방법.
- 이소펜테닐 피로포스페이트, 알릴 피로포스페이트, 및 거의 순수한 고무폴리머라제를 함유하는 반응 혼합물을 형성시키고, 천연고무를 생성시키기에 충분한 시간동안 반응혼합물을 항온 처리한 다음, 천연고무를 회수하는 단계들로 이루어진 천연고무의 제조방법.
- 제36항에 있어서, 언급된 천연고무가 고분자 천연 고무인 방법.
- 제36항에 있어서, 언급된 알릴 피로포스페이트가 디메틸알릴 피로포스페이트, 고분자 천연 고무 입자, 및 저분자 천연고무 입자중에서 선택되는 방법.
- 제36항에 있어서, 언급된 반응 혼합물이, 완충제, 2가 금속이온, 중금속 킬레이트제, 및 폴리머라 제용 안정화제를 함유하는 방법
- 제36항에 있어서, 언급된 반응 혼합물이, 완충제, 염화마그네숨, 이칼륨 EDTA, 및 술프하이드릴 화합물을 추가로 함유하는 방법.
- 제40항에 있어서, 언급된 완충제가 0.1 내지 0.6M 트리스 클로라이드로 이루어지는 방법.
- 제40항에 있어서, 염화마그네슘의 농도가 약 1mM 내지 약 10mM이고, 이칼륨 EDTA의 농도가 약 5mM 내지 약 10mM이며, 술프하이드릴 화합물이 약 0mM 내지 약 10mM 농도의 글루타티온인 방법.
- 제36항에 있어서, 언급된 반응혼합물의 pH가 약 5 내지 약 10인 방법.
- 제36항에 있어서, 언급된 반응혼합물의 pH가 약 7내지 약 7인 방법.
- 제36항에 있어서, 언급된 항온 처리를 약 4℃ 내지 약 60℃의 온도에서 수행하는 방법.
- 제36항에 있어서, 언급된 항온처리를 약 30℃의 온도에서 수행하는 방법.
- 제36항에 있어서, 언급된 항온처리를 약 1시간 내지 약 16시간동안 수행하는 방법.
- 제36항에 있어서, 언급된 항온처리를 약 4시간 내지 약 8시간동안 수행하는 방법.
- 제36항의 방법에 의해 제조된 고분자 천연고무.
- 이소펜테닐 피로포스페이트와 하나이상의 다른 공중합성단량체, 알릴 피로포스페이트, 및 거의 순수한 고무폴리머라제를 함유하는 반응 혼합물을 형성시키고, 공중합체를 생성시키기에 충분한 시간동안 반응혼합물을 항온처리한 다음, 공중합체를 회수하는 단계들로 이루어진, 공중합체의 제조방법.
- 제50항의 방법에 의해 제조된 공중합체.
- 이소펜테닐 피로포스페이트, 이소펜테닐 피로포스페이트아이소머라제, 2가 금속이온, 포스페이타제억제제, 및 완충제를 함유하는 반응 혼합물을 형성시키고, 디메틸알릴피로 포스페이트를 생성시키기에 충분한 시간동안 반응혼합물을 항온처리한 다음, 디메틸알릴 피로포스페이트를 회수하는 단계들로 이루어진, 디메티알릴 피로포스페이트의 제조방법.
- 제52항에 있어서, 2가 금속 이온이 Mgtt이고, 포스페이타제억제제가 플루오르화 나트륨이며, 완충제가 중탄산나트륨이고, 항온처리를 약 1 내지 2시간 동안 수행하는 방법.
- 제52항에 있어서, 언급된 항온처리를 약 35 내지 40℃의 온도에서 수행하는 방법.
- 제52항에 있어서, 언급된 디메틸알릴 피로포스페이트를, 10,000분자량 컷 오프(cut off)를 갖는 막을 통해 항온 처리된 혼합물을 여과시킴으로써 회수하는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US720740 | 1985-04-08 | ||
| US06/720,740 US4638028A (en) | 1985-04-08 | 1985-04-08 | Rubber polymerases and methods for their production and use |
| PCT/US1986/000480 WO1986006095A1 (en) | 1985-04-08 | 1986-03-07 | Rubber polymerases and methods for their production and use |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| KR880700064A true KR880700064A (ko) | 1988-02-15 |
Family
ID=24895111
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR860700866A KR880700064A (ko) | 1985-04-08 | 1986-03-07 | 고무 폴리머라제 및 이의 제법과 용도 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US4638028A (ko) |
| EP (1) | EP0216855A4 (ko) |
| JP (1) | JPS62502797A (ko) |
| KR (1) | KR880700064A (ko) |
| CN (2) | CN1004145B (ko) |
| AU (2) | AU601147B2 (ko) |
| BR (1) | BR8606536A (ko) |
| IL (1) | IL78337A (ko) |
| IN (1) | IN170383B (ko) |
| WO (1) | WO1986006095A1 (ko) |
| ZA (1) | ZA861928B (ko) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016178553A1 (ko) * | 2015-05-07 | 2016-11-10 | 한국생명공학연구원 | 천연고무 중합효소 유전자 및 이의 용도 |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4983729A (en) * | 1989-10-19 | 1991-01-08 | The Goodyear Tire & Rubber Company | DNA fragment encoding a rubber polymerase and its use |
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5563241A (en) * | 1995-03-15 | 1996-10-08 | Guthrie Foundation For Education And Research | Methods to remove proteins from natural rubber latex |
| US6054525A (en) * | 1996-09-16 | 2000-04-25 | The University Of Akron | Hypoallergenic natural rubber latex and a process for making the same |
| US5962147A (en) * | 1996-11-26 | 1999-10-05 | General Latex And Chemical Corporation | Method of bonding with a natural rubber latex and laminate produced |
| US6316695B1 (en) * | 1999-04-22 | 2001-11-13 | Korea Kumho Petrochemical Co., Ltd. | Isopentenyl diphosphate isomerase from Hevea brasiliensis and rubber producing method using the same |
| US6380283B1 (en) | 1999-11-23 | 2002-04-30 | Tillotson Healthcare Corporation | Enzyme, stabilizer and antioxidant treated natural rubber latex product and method of processing same |
| US20090099309A1 (en) * | 2007-10-16 | 2009-04-16 | Yulex Corporation | Guayule resin multipolymer |
| ES2447371T3 (es) | 2008-04-14 | 2014-03-11 | Bridgestone Corporation | Proceso para recuperar caucho a partir de látex de caucho natural |
| DE102008038000A1 (de) * | 2008-08-16 | 2010-02-18 | Continental Reifen Deutschland Gmbh | Verfahren zur Modifikation von Naturkautschuk und modifizierter Naturkautschuk |
| WO2010053553A1 (en) * | 2008-11-06 | 2010-05-14 | University Of Akron | Biosynthesis of polyisoprenoids |
| EP3412305B1 (en) | 2011-06-10 | 2021-01-06 | Baxalta GmbH | Treatment of coagulation disease by administration of recombinant vwf |
| JP6004249B2 (ja) * | 2011-12-28 | 2016-10-05 | 住友ゴム工業株式会社 | イソプレノイドの製造方法、及びイソプレノイド |
| JP5950253B2 (ja) * | 2012-02-13 | 2016-07-13 | 住友ゴム工業株式会社 | イソプレノイドの製造方法、及びイソプレノイド |
| CN104271606B (zh) | 2012-03-06 | 2019-07-09 | 株式会社普利司通 | 用于从经陈化的压块收取橡胶的方法和包含来自非三叶胶植物的植物物质的经陈化的压块 |
| US10023660B2 (en) | 2012-05-16 | 2018-07-17 | Bridgestone Corporation | Compositions containing purified non-hevea rubber and related purification methods |
| US10138304B2 (en) | 2012-06-18 | 2018-11-27 | Bridgestone Corporation | Methods for increasing the extractable rubber content of non-Hevea plant matter |
| WO2013192182A1 (en) | 2012-06-18 | 2013-12-27 | Bridgestone Corporation | Systems and methods for the management of waste associated with processing guayule shrubs to extract rubber |
| WO2013192227A1 (en) | 2012-06-18 | 2013-12-27 | Bridgestone Corporation | Methods for desolventization of bagasse |
| WO2015038707A1 (en) | 2013-09-11 | 2015-03-19 | Bridgestone Corporation | Processes for the removal of rubber from tks plant matter |
| EP2848693A1 (de) * | 2013-09-12 | 2015-03-18 | LANXESS Deutschland GmbH | Verkettung von halogenierten Alkenyldiphosphat-Derivaten |
| CN107522913A (zh) * | 2017-09-19 | 2017-12-29 | 润益康地（上海）物资贸易有限公司 | 一种天然胶乳与白炭黑液相共沉混合胶的制备方法 |
| US10775105B2 (en) | 2018-11-19 | 2020-09-15 | Bridgestone Corporation | Methods for the desolventization of bagasse |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3214398A (en) * | 1961-06-19 | 1965-10-26 | Du Pont | Emulsion paint comprising tung oil and lead silicate |
| US3442845A (en) * | 1966-11-04 | 1969-05-06 | Borden Co | Hot and cold water redispersible polyvinyl acetate adhesives |
| US4055529A (en) * | 1975-04-29 | 1977-10-25 | Tile Council Of America, Inc. | Adhesive joint dressing compositions containing an alkali thickenable polymer |
| US4472540A (en) * | 1982-07-14 | 1984-09-18 | American Olean Tile Company | Wide joint latex grout composition |
-
1985
- 1985-04-08 US US06/720,740 patent/US4638028A/en not_active Expired - Fee Related
-
1986
- 1986-03-07 EP EP19860902137 patent/EP0216855A4/en not_active Ceased
- 1986-03-07 JP JP61501659A patent/JPS62502797A/ja active Pending
- 1986-03-07 BR BR8606536A patent/BR8606536A/pt not_active Application Discontinuation
- 1986-03-07 KR KR860700866A patent/KR880700064A/ko not_active Application Discontinuation
- 1986-03-07 WO PCT/US1986/000480 patent/WO1986006095A1/en not_active Application Discontinuation
- 1986-03-07 AU AU55812/86A patent/AU601147B2/en not_active Ceased
- 1986-03-14 ZA ZA861928A patent/ZA861928B/xx unknown
- 1986-03-14 IN IN242/DEL/86A patent/IN170383B/en unknown
- 1986-03-30 IL IL78337A patent/IL78337A/xx not_active IP Right Cessation
- 1986-04-08 CN CN86102341.2A patent/CN1004145B/zh not_active Expired
-
1988
- 1988-02-01 CN CN198888100484A patent/CN88100484A/zh active Pending
-
1990
- 1990-07-06 AU AU58763/90A patent/AU5876390A/en not_active Abandoned
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016178553A1 (ko) * | 2015-05-07 | 2016-11-10 | 한국생명공학연구원 | 천연고무 중합효소 유전자 및 이의 용도 |
Also Published As
| Publication number | Publication date |
|---|---|
| IL78337A0 (en) | 1986-07-31 |
| CN86102341A (zh) | 1986-10-15 |
| CN1004145B (zh) | 1989-05-10 |
| AU5581286A (en) | 1986-11-05 |
| ZA861928B (en) | 1986-10-29 |
| WO1986006095A1 (en) | 1986-10-23 |
| EP0216855A4 (en) | 1989-04-12 |
| IL78337A (en) | 1991-04-15 |
| JPS62502797A (ja) | 1987-11-12 |
| US4638028A (en) | 1987-01-20 |
| BR8606536A (pt) | 1987-08-04 |
| EP0216855A1 (en) | 1987-04-08 |
| CN88100484A (zh) | 1988-08-10 |
| IN170383B (ko) | 1992-03-21 |
| AU601147B2 (en) | 1990-09-06 |
| AU5876390A (en) | 1990-10-25 |
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