KR850008099A - 모노클로날 항체의 정제방법 - Google Patents
모노클로날 항체의 정제방법 Download PDFInfo
- Publication number
- KR850008099A KR850008099A KR1019850002671A KR850002671A KR850008099A KR 850008099 A KR850008099 A KR 850008099A KR 1019850002671 A KR1019850002671 A KR 1019850002671A KR 850002671 A KR850002671 A KR 850002671A KR 850008099 A KR850008099 A KR 850008099A
- Authority
- KR
- South Korea
- Prior art keywords
- monoclonal antibody
- sample
- essentially
- silica gel
- microns
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S424/00—Drug, bio-affecting and body treating compositions
- Y10S424/806—Drug, bio-affecting and body treating compositions involving IgM
- Y10S424/807—Monoclonal
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/863—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof involving IgM
- Y10S530/864—Monoclonal
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Medicinal Chemistry (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (5)
- 크로마로그라피 충전은 풀리에틸렌이핀 기능이 비-가교 공유 결합형태로, 또는 흡착되 가교결합 형태로 결합된 평균 입경이 약 3 내지 약 40미크론이고 평균 기공크기가 약 50 내지 약 1000옹스트롬(Å)단위인 미립사상 실리카겔로 필수적으로 구성되는 액체크로마토그라피 방법을 사용함으로써 시료로부터 모노클로날 항체를 분리시키고 회수하는 것으로 이루어지는 것을 특징으로 하는 필수적으로 균일한 모노클로날 항체 IgM 형을 이 모노클로날 항체를 포함하는 쥐 복수액 시료로부터 얻는 방법.
- 크로마토그라피 충전은 평균 입경이 약 3내지 약 40미크론이고 평균 기공크기가 약 50 내지 약 1000옹스트롬(Å)단위인 미립자상 실리카겔과, 평균 분자량이 약 400 내지 약 1800인 폴리에틸렌 이미노프로필 트리메특시 실란과의 반응생성물로 필수적으로 구성되는 액체 크로마토그라피 방법을 사용함으로써 시료로부터 모노클로날 항체를 분리시키고 회수하는 것으로 이루어지는 것을 특징으로 하는 필수적으로 균일한 모노클로날 항체 IgM 형을 상기 모노클로날 항체로 포함하는 쥐 복수액 시료로부터 얻는 방법.
- a. 잇다른 크로마토그라피 분리와 회수단계에서 기울기 용리에 사용된 낮은 이온강도 완충액의 이온강도와 같거나 보다 낮은 이온강도, 그리고 모노클로날 항체의 pI보다 더 큰 pH로 무-미립자 쥐복수액의 시료를 평형화 시키는 단계와, b. 크로마토그라피 충전은 평균 입경이 약 3 내지 약 40 미크론이고 평균 기공크기가 약 50 내지 1000옹 스트롬(Å)단위인 미립자상 실리카겔과, 평균 분자량이 약 400 내지 약 1800인 폴리에틸텐이미노프로필 트리메톡시 실탄과의 반응생성물로 필수적으로 구성되는 액체 크로마토그라피 방법을 사용함으로써 시료로부터 모노클로날 항체 IgM 형을 분리시키고 회수하는 단계로, 이루어지는 것을 특징으로 하는 필수적으로 정제된 모노클로날 항체 IgM 형을 상기 모노클로날 항체를 포함하는 무-미립자 쥐 복수액의 시료로부터 얻는 방법.
- 크로마토그라피 충전은 플리에틸렌이민 기능이 흡착된 가교결합 형태로 결합된 평균입경이 약 3 내지 약 40 미크론이고 평균 기공크기가 약 50 내지 약 1000 옹스트롬(Å)단위인 미립자상 실리카겔로 필수적으로 구성되는 액체 크로마토그라피 방법을 사용함으로써 시료로부터 모노클로날 항체를 분리시키고 회수하는 것으로 이루어지는 것을 특징으로 하는 필수적으로 균일한 모노클로날 항체 IgM 형을 이 모노클로날 항체를 포함하는 쥐 복수액 시료로부터 얻는 방법.
- a. 잇다른 크로마토그라피 분리와 회스단계에서 기울기 용리에 사용된 낮은 이온강도 완충액의 이온강도와 같거나 보다 낮은 이온강도, 그리고 모노클로날 항체의 pI 보다 더 큰 pH로 무-미립자 쥐 복수액의 시료를 평형화시키는 단계와, b. 크로마토그라피 충전은 플리에틸렌이민 기능이 흡착된 가교결합 형태로 결합된 평균입경이 약 3 내지 약 40미크론이고 평균 기공크기가 약 50 내지 약 1000 옹스트롬(Å)단위인 미립자상 실리카겔로 필수적으로 구성되는 액체 크로마토그라피 방법을 사용함으로써 시료로부터 모노클로날 항체 IgM 형을 분리시키고 회수하는 단계로 이루어지는 것을 특징으로 하는 필수적으로 정제된 모노클로날 항체 IgM 형을 이 모노클로날 항체를 포함하는 무-미립자 쥐 복수액의 시료로부터 얻는 방법.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US613248 | 1984-05-23 | ||
| US06/613,248 US4604235A (en) | 1984-05-23 | 1984-05-23 | Purification of monoclonal antibodies |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| KR850008099A true KR850008099A (ko) | 1985-12-13 |
| KR870001434B1 KR870001434B1 (ko) | 1987-08-06 |
Family
ID=24456492
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| KR1019850002671A KR870001434B1 (ko) | 1984-05-23 | 1985-04-20 | 모노클로날 항체의 정제방법 |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US4604235A (ko) |
| EP (1) | EP0162462B1 (ko) |
| JP (1) | JPS60260593A (ko) |
| KR (1) | KR870001434B1 (ko) |
| AT (1) | ATE48615T1 (ko) |
| AU (1) | AU576713B2 (ko) |
| CA (1) | CA1218948A (ko) |
| DE (1) | DE3574709D1 (ko) |
| IL (1) | IL75006A (ko) |
| NZ (1) | NZ211813A (ko) |
| ZA (1) | ZA852803B (ko) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2571745B1 (fr) * | 1984-10-17 | 1988-10-28 | Trest Juzhvodoprovod | Anticorrosif pour proteger la surface interne d'une canalisation contre la corrosion, procede d'obtention de cet anticorrosif et procede de protection de la surface interne d'une canalisation contre la corrosion utilisant cet anticorrosif |
| US4606825A (en) * | 1985-04-22 | 1986-08-19 | J. T. Baker Chemical Company | Purification of immunoglobulin G |
| JPS6419024A (en) * | 1987-07-15 | 1989-01-23 | Tosoh Corp | Separation of immunoglobulin g subclass |
| JPH01503808A (ja) * | 1987-07-16 | 1989-12-21 | イー・アイ・デユポン・ド・ネモアース・アンド・コンパニー（インコーポレイテツド） | 固定化された凝集剤を用いるアフイニテイ分離 |
| US5157113A (en) * | 1987-08-10 | 1992-10-20 | Miles Inc. | Removal of nucleic acids from monoclonal antibody preparations |
| ES2045027T3 (es) * | 1987-08-10 | 1994-01-16 | Miles Inc | Igm purificadas. |
| IL90281A (en) * | 1988-06-06 | 1994-10-07 | Miles Inc | Preparations containing MGI antibodies |
| DE3825429C2 (de) * | 1988-07-27 | 1994-02-10 | Biotest Pharma Gmbh | Verfahren zur Herstellung eines intravenös verabreichbaren polyklonalen Immunglobulin-Präparates mit hohem IgM-Gehalt |
| US5126250A (en) * | 1988-09-28 | 1992-06-30 | Eli Lilly And Company | Method for the reduction of heterogeneity of monoclonal antibodies |
| US5759864A (en) * | 1995-06-23 | 1998-06-02 | Cedars Sinai Medical Center | Methods for reducing background binding in antibody preparations |
| JP4728140B2 (ja) * | 2005-02-21 | 2011-07-20 | 国立大学法人九州大学 | タンパク質の固定化方法 |
| KR100795945B1 (ko) * | 2005-08-24 | 2008-01-21 | 가부시키가이샤 엔.티.티.도코모 | 송신 전력 제어 방법 및 무선 네트워크 제어국 |
| US8580935B2 (en) | 2007-02-26 | 2013-11-12 | Alltech Associates, Inc. | Ultra-fast chromatography |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4196265A (en) * | 1977-06-15 | 1980-04-01 | The Wistar Institute | Method of producing antibodies |
| CA1142466A (en) * | 1979-01-09 | 1983-03-08 | Cesar Milstein | Cell lines |
| US4381295A (en) * | 1979-04-26 | 1983-04-26 | Ortho Pharmaceutical Corporation | Monoclonal antibody to human helper T cells and methods of preparing same |
| US4271145A (en) * | 1979-10-22 | 1981-06-02 | The Massachusetts General Hospital | Process for producing antibodies to hepatitis virus and cell lines therefor |
| US4361550A (en) * | 1979-12-04 | 1982-11-30 | Ortho Pharmaceutical Corporation | Complement-fixing monoclonal antibody to human suppressor T cells and methods of preparing same |
| US4423147A (en) * | 1980-04-11 | 1983-12-27 | Secher David S | Monoclonal antibody to interferon-α |
| US4427653A (en) * | 1981-01-26 | 1984-01-24 | President And Fellows Of Harvard College | Method of making monoclonal antibodies |
| US4361509A (en) * | 1981-12-14 | 1982-11-30 | Scripps Clinic And Research Foundation | Ultrapurification of factor VIII using monoclonal antibodies |
| JPS5929622A (ja) * | 1982-08-10 | 1984-02-16 | Meiji Seika Kaisha Ltd | モノクロ−ナル抗体、その製造法およびその用途 |
| US4469630A (en) * | 1983-11-25 | 1984-09-04 | J. T. Baker Chemical Co. | Purification of monoclonal antibodies |
-
1984
- 1984-05-23 US US06/613,248 patent/US4604235A/en not_active Expired - Lifetime
-
1985
- 1985-04-15 CA CA000479140A patent/CA1218948A/en not_active Expired
- 1985-04-15 ZA ZA852803A patent/ZA852803B/xx unknown
- 1985-04-16 NZ NZ211813A patent/NZ211813A/xx unknown
- 1985-04-19 AU AU41446/85A patent/AU576713B2/en not_active Ceased
- 1985-04-20 KR KR1019850002671A patent/KR870001434B1/ko not_active IP Right Cessation
- 1985-04-24 IL IL75006A patent/IL75006A/xx unknown
- 1985-05-21 JP JP60107097A patent/JPS60260593A/ja active Granted
- 1985-05-22 EP EP85106307A patent/EP0162462B1/en not_active Expired
- 1985-05-22 DE DE8585106307T patent/DE3574709D1/de not_active Expired - Lifetime
- 1985-05-22 AT AT85106307T patent/ATE48615T1/de not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| AU4144685A (en) | 1985-11-28 |
| JPH0144315B2 (ko) | 1989-09-27 |
| US4604235A (en) | 1986-08-05 |
| ATE48615T1 (de) | 1989-12-15 |
| DE3574709D1 (de) | 1990-01-18 |
| AU576713B2 (en) | 1988-09-01 |
| IL75006A0 (en) | 1985-08-30 |
| JPS60260593A (ja) | 1985-12-23 |
| CA1218948A (en) | 1987-03-10 |
| EP0162462B1 (en) | 1989-12-13 |
| EP0162462A2 (en) | 1985-11-27 |
| IL75006A (en) | 1989-09-28 |
| KR870001434B1 (ko) | 1987-08-06 |
| NZ211813A (en) | 1988-08-30 |
| EP0162462A3 (en) | 1987-05-13 |
| ZA852803B (en) | 1986-02-26 |
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