KR850003162A - 인체 γ-인터페론 단량체의 제조방법 - Google Patents

인체 γ-인터페론 단량체의 제조방법 Download PDF

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KR850003162A
KR850003162A KR1019840006083A KR840006083A KR850003162A KR 850003162 A KR850003162 A KR 850003162A KR 1019840006083 A KR1019840006083 A KR 1019840006083A KR 840006083 A KR840006083 A KR 840006083A KR 850003162 A KR850003162 A KR 850003162A
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interferon
human
gel
protein
compound
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KR1019840006083A
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KR910006603B1 (ko
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기요시 나라 (외 1)
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구라바야시 이꾸시로
다께다 야꾸힝 고오교 가부시기 가이샤
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    • C07—ORGANIC CHEMISTRY
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    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C07K1/1133—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by redox-reactions involving cystein/cystin side chains
    • A—HUMAN NECESSITIES
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    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
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    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/811—Interferon
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S930/00—Peptide or protein sequence
    • Y10S930/01—Peptide or protein sequence
    • Y10S930/14—Lymphokine; related peptides
    • Y10S930/142—Interferon

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Abstract

내용 없음

Description

인체 γ-인터페론 단량체의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 rIFN-γ의 일예로서 폴리펩티드(Ⅰ)의 아미노산 서열을 나타낸 것이고,
제2도는 참고예 2(I)에 기재된 플라스미드 pHIThp 1101-d2의 형성도식을 나타낸 것이다.

Claims (15)

  1. 조인체γ-인터페론을 환원 황화합물 및 단백질 반성제의 공존하에 겔여과함을 특징으로 하는 인체γ-인터페론 단량제의 제조방법.
  2. 제1항에 있어서, 환원한 화합물이 유기황 화합물인 방법.
  3. 제1항에 있어서, 유기황 화합물이 시스테인, N-아세틸시스테인, N-아세킬호모시스테인, 글루타티온(환원형), 티오에탄올아민, 모노티오글리세롤, 디티오트레이톨, 1∼7 탄소원자를 갖는 티오 알칸 및 포름알테히드 소듐 술폭실레이트 디히드레이트의 균에서 선택된 화합물인 방법.
  4. 제3항에 있어서, 유기황 화합물이 글루타티온(환원형)인 방법.
  5. 제1항에 있어서, 단백질 변성제가 구아니딘염, 우레아 및 티오시아네이트의 균에서 선택된 것인 방법.
  6. 제5항에 있어서, 단백질 변성제가 구아니딘염인 방법.
  7. 제1항에 있어서, 인체γ-인터페론이 제조합 인체γ-인터페론인 방법.
  8. 제1항에 있어서, 조인체 γ-인터페론이 인체γ-인터페론을 함유한 추출물을 항체컬럼을 통과시킨 용출물인 방법.
  9. 제1항에 있어서, 겔이 덱스트란, 폴리아크릴아미드 및 아가로즈의 군에서 선택된 것인 방법.
  10. 제9항에 있어서, 겔이 덱스트란인 방법.
  11. 제1항에 있어서,겔여과가 환원황화합물 및 단백질 변성제를 함유한 완충용액에 용해된 조인체γ-인터페론을 과립겔로 패팅된 컬럼에 부하시키고, 같은 완충용액으로 γ-인터페론을 용출시킴으로써 수행되는 방법.
  12. 제11항에 있어서, 완충용액이 각각 1∼100mM 및 0.1∼7M 농도의 환원황 화합물 및 단백질 변성제를 함유하는 방법.
  13. 제11항에 있어서, 완충 용액이 pH5.0∼8.0인 방법.
  14. 제11항에 있어서, 용출인 0.1∼10의 공감속도로 수행되는 방법.
  15. 제1항에 있어서, 생성된 인체γ-인터페론 단량체 용액을 저분자량 화합물을 제거하기에 적당한 겔을 사용하에 더 겔여과 함으로써 환원황 화합물 및 단백질 변성제가 없는 인체γ-인터페론 단량제를 수득하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840006083A 1983-10-04 1984-10-02 인체 γ-인터페론 단량체의 제조방법 KR910006603B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP186383/83 1983-10-04
JP58186383A JPS6079000A (ja) 1983-10-04 1983-10-04 ヒトγ型インタ−フエロン単量体の製造法
JP186383 1983-10-04

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KR850003162A true KR850003162A (ko) 1985-06-13
KR910006603B1 KR910006603B1 (ko) 1991-08-29

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US (1) US4686284A (ko)
EP (1) EP0136694B1 (ko)
JP (1) JPS6079000A (ko)
KR (1) KR910006603B1 (ko)
CN (1) CN85101907A (ko)
AT (1) ATE49981T1 (ko)
AU (1) AU572196B2 (ko)
CA (1) CA1239094A (ko)
DE (1) DE3481193D1 (ko)
DK (1) DK474384A (ko)
ES (1) ES8506195A1 (ko)
GR (1) GR80545B (ko)
IL (1) IL73166A (ko)
NZ (1) NZ209758A (ko)
PH (1) PH20251A (ko)
PT (1) PT79298B (ko)
ZA (1) ZA847501B (ko)

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MX9203641A (es) * 1983-12-16 1992-07-01 Genentech Inc Interferones gamma recombinantes que poseen estabilidad mejorada y metodos biotecnologicos para su obtencion.
US4855238A (en) * 1983-12-16 1989-08-08 Genentech, Inc. Recombinant gamma interferons having enhanced stability and methods therefor
IL74094A0 (en) * 1984-01-23 1985-04-30 Takeda Chemical Industries Ltd Highly solubilised protein and production thereof
GB8414354D0 (en) * 1984-06-05 1984-07-11 Biogen Nv Purifying protein
GB8508340D0 (en) * 1985-03-29 1985-05-09 Creighton T E Production of protein
US4873312A (en) * 1985-04-25 1989-10-10 Amgen Method for purifying interferon and composition of matter produced thereby
US4766205A (en) * 1985-11-13 1988-08-23 Beatrice Companies, Inc. Method for isolation of recombinant polypeptides in biologically active forms
US4950470A (en) * 1986-10-06 1990-08-21 Board Of Regents, The University Of Texas System Methods and compositions employing interferon-gamma
ES2119779T3 (es) * 1990-09-05 1998-10-16 Southern Cross Biotech Pty Ltd Solubilizacion de proteinas en formas activas.
AU648214B2 (en) * 1991-12-31 1994-04-14 Lucky Limited Recombinant gene coding for human alpha interferon and expression vector thereof, etc.
US5434249A (en) * 1992-05-27 1995-07-18 Viragen Inc. Method for modulating specific activity of inteferon alpha
JP3452779B2 (ja) * 1997-11-28 2003-09-29 株式会社東海理化電機製作所 回転量制限装置及びウエビング巻取装置

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* Cited by examiner, † Cited by third party
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NL7404589A (nl) * 1974-04-03 1975-10-07 Stichting Rega V Z W Werkwijze voor het stabiliseren van interferon.
US4100150A (en) * 1975-11-04 1978-07-11 G. D. Searle & Co. Stabilization of interferon against mechanical stress using thioctic acid
US4278661A (en) * 1979-10-12 1981-07-14 E. I. Du Pont De Nemours And Company Purification of interferon
AU561343B2 (en) * 1981-10-19 1987-05-07 Genentech Inc. Human immune interferon by recombinant dna
DE3273597D1 (en) * 1981-11-28 1986-11-06 Sunstar Kk Pharmaceutical composition containing interferon in stable state
CA1210714A (en) * 1982-03-23 1986-09-02 Alan Sloma .alpha.-INTERFERON GX-1
US4432895A (en) * 1982-11-24 1984-02-21 Hoffmann-La Roche Inc. Monomeric interferons
US4476049A (en) * 1983-09-20 1984-10-09 Hoffmann-La Roche Inc. Method for the extraction of immune interferon

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AU572196B2 (en) 1988-05-05
EP0136694B1 (en) 1990-01-31
CA1239094A (en) 1988-07-12
GR80545B (en) 1985-02-05
ATE49981T1 (de) 1990-02-15
JPH0428279B2 (ko) 1992-05-13
PT79298B (en) 1986-11-13
JPS6079000A (ja) 1985-05-04
NZ209758A (en) 1988-02-29
DE3481193D1 (de) 1990-03-08
IL73166A (en) 1989-02-28
KR910006603B1 (ko) 1991-08-29
DK474384D0 (da) 1984-10-03
ZA847501B (en) 1986-05-28
PH20251A (en) 1986-11-10
PT79298A (en) 1984-11-01
IL73166A0 (en) 1985-01-31
EP0136694A3 (en) 1986-07-16
ES536471A0 (es) 1985-07-01
EP0136694A2 (en) 1985-04-10
AU3380484A (en) 1985-04-18
US4686284A (en) 1987-08-11
ES8506195A1 (es) 1985-07-01
DK474384A (da) 1985-04-05
CN85101907A (zh) 1987-01-10

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