KR850000524A - 세포증식 촉진작용을 가지는 단백질의 제조방법 - Google Patents

세포증식 촉진작용을 가지는 단백질의 제조방법 Download PDF

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KR850000524A
KR850000524A KR1019840001951A KR840001951A KR850000524A KR 850000524 A KR850000524 A KR 850000524A KR 1019840001951 A KR1019840001951 A KR 1019840001951A KR 840001951 A KR840001951 A KR 840001951A KR 850000524 A KR850000524 A KR 850000524A
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protein
cell proliferation
producing
molecular weight
promoting protein
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세쓰로우 후지이 (외 3)
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다까기 지로우
마루호 가부시기 가이샤
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Abstract

내용 없음.

Description

세포증식 촉진작용을 가지는 단백질의 제조방법
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1도는 세포증식에 대한 WHS의 작용을 표시하는 그래프이고 0인 표시의 그래프는 대조균이고 표시의 그래프는 WHS첨가균을 표시한다. 종좌표는 세포수, 횡자표는 배양일수이다.

Claims (6)

  1. 본 발명은 세포증식촉진 단백질을 생산할 수 있는 균을 배양하고 또한 누클에오시드 풔스풔트란스 레라아제를 실질적으로 포함유하지 않는 것으로서 형성되는 단백질을 추출 분리하는 것으로 구성되는 단백질 제조방법인데 하기 특징을 가진다. (a) 상술한 배양균체 또는 배양액에서 분자량이 리오글로빈(분자량 670,000) 소 r-글로불린(분자량 158,000) 말미오글로빈(분자량 17,000) 비타민 12(분자량 1,350)을 마아카아 단백질로서 고속크로마토그래피로 얻어지는 용출량과 분자량의 관계선으로 부터 구했을때에 약 5,000-160,000이고, (b) 산성 및 중성아미노산에 의해서 주로 구성되고 또한 산성 아미노산으로서 글루타민산과 아스파라긴산이 비교적 다량으로 함유되고, (c) 세포증식촉진 작용을 가지는 것을 특징으로 하는 세포증식 촉진 단백질을 생산하는 방법.
  2. 제1항에 있어서 클로스트리듐속, 스타필로콕커스속, 사리시나속, 바틸루스속, 아에로콕커스속, 스트랩토콕커스속, 아시네트백터속, 코리네박테륨속, 락토바칠루스속, 세라티아속, 에세리커아속, 살모넬라속, 프로튜스속, 슈도모나스속, 아에로모나스속, 플라보박테륨속 등에서 전술한 균체가 선정되서 세포증식촉진단백질을 생산할 수 있는 것을 특징으로 하는 세포증식 촉진 단백질을 생산하는 방법.
  3. 제2항에 있어서, 세포증식 촉진 단백질을 생산할 수 있는 균이 클로스트리듐, 페리프린겐스인 청구 범위 제2항에 기재한 세포증식 촉진 단백질을 생산하는 방법.
  4. 제2항에 있어서, 세포 증식 촉진 단백질을 생산할 수 있는 균이 스타필로 콕커스·아우류스, 스타필로콕커스·에피더미디스, 사리시나·루테아, 바칠루스·섭틸리스, 아에로콕커스·비리단스, 스트랩토콕커스·페카리스, 아시네트백터·칼코아세티크스, 코리네박테륨·패시안스, 세라디아·마르세센스, 에세리키아·콜리, 살모넬라·엔테리티디스, 프로튜스 불가리스, 프로튜스·미라빌리스, 슈와도모나스·말토필라, 아에로모나스·히드로필라, 플라보박테늄·루테스센스인 세포 증식 촉진 단백질을 생산하는 방법.
  5. 제1항 내지 제3항에 있어서, 추출 분리공정에 있어서 단백질 분해 효소처리를 함유하는 세포 증식 촉진 단백질을 생산하는 방법.
  6. 제5항에 있어서, 단백질 가수분해 효소가 트리프신 혹은 그것과 기타의 단백질 가수분해 효소와의 혼합물인 세포 증식 촉진 단백질을 생산하는 방법.
    ※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019840001951A 1983-06-30 1984-04-13 세포증식 촉진작용을 가지는 단백질의 제조방법 KR850000524A (ko)

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Families Citing this family (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3346953A1 (de) * 1983-12-24 1985-08-14 Organogen Medizinisch-Molekularbiologische Forschungsgesellschaft mbH, 6900 Heidelberg Cardiodilatin, ein neues peptidhormon und verfahren zu seiner herstellung
FR2565985B1 (fr) * 1984-06-19 1987-09-25 Rhone Poulenc Sante Nouvelles substances biologiquement actives obtenues a partir de la caseine bovine, leur procede de preparation et les compositions qui les contiennent
US4746729A (en) * 1986-07-30 1988-05-24 Kuettner Klaus E Cartilage-derived leukocyte elastase-inhibitor
SE8604587D0 (sv) * 1986-10-28 1986-10-28 Pharmacia Ab Lekemedelssats for sarlekningsstimulering samt sett att anvenda och tillverka satsen
US5019559A (en) * 1986-11-14 1991-05-28 President And Fellows Of Harvard College Wound healing using PDGF and IGF-II
US4861757A (en) * 1986-11-14 1989-08-29 Institute Of Molecular Biology Wound healing and bone regeneration using PDGF and IGF-I
ATE80307T1 (de) * 1986-12-11 1992-09-15 British Tech Group Verbesserungen im gebrauch oder in bezug auf den gebrauch von egf.
GB8713169D0 (en) * 1987-06-05 1987-07-08 Peters T J Uptake of iron by body
JPH03503117A (ja) * 1988-02-12 1991-07-18 ハイテク　レセプター　アーベー 免疫グロブリン結合性物質そのサブフラグメント、それらの製造方法、試薬キットおよび免疫グロブリン結合性物質
US4983581A (en) * 1988-05-20 1991-01-08 Institute Of Molecular Biology, Inc. Wound healing composition of IGF-I and TGF-β
US5256644A (en) * 1988-05-20 1993-10-26 Institute Of Molecular Biology, Inc. Wound healing using IGF-II and TGF
US5006509A (en) * 1988-06-24 1991-04-09 Harald Waago Use of human growth hormone in treating topical ulcers
US5034375A (en) * 1988-08-10 1991-07-23 Institute Of Molecular Biology, Inc. Process of wound healing using PDGF and EGF
US5175147A (en) * 1988-08-19 1992-12-29 Takeda Chemical Industries, Ltd Acid-resistant fgf composition and method of treating ulcerating diseases of the gastrointestinal tract
US5051443A (en) * 1988-10-12 1991-09-24 Eye Research Institute Of Retina Foundation, Inc. Method for enhancing healing of corneal endothelial wounds
US5036046A (en) * 1988-10-12 1991-07-30 Eye Research Institute Of Retina Foundation, Inc. Method for enhancing healing of corneal endothelial wounds
US5032575A (en) * 1988-10-12 1991-07-16 Eye Research Institute Of Retina Foundation, Inc. Method for enhancing healing of corneal endothelial wounds
FR2647784B1 (fr) * 1989-06-06 1991-10-04 Grimberg Georges Association de vitamine a a dose physiologique et de differents principes actifs ayant une activite therapeutique
US5206023A (en) * 1991-01-31 1993-04-27 Robert F. Shaw Method and compositions for the treatment and repair of defects or lesions in cartilage
US5149691A (en) * 1991-03-12 1992-09-22 Creative Biomolecules, Inc. Issue repair and regeneration through the use of platelet derived growth factor (pdgf) in combination with dexamethasone
US5270300A (en) * 1991-09-06 1993-12-14 Robert Francis Shaw Methods and compositions for the treatment and repair of defects or lesions in cartilage or bone
US5618544A (en) * 1992-08-12 1997-04-08 Bays-Brown Dermatologics, Inc. Method of decreasing cutaneous senescence
EP0624642B1 (en) * 1993-05-12 1999-01-20 Indian Council For Medical Research Novel thrombolytic agent, process for preparing same and its use for the preparation of a thrombi dissolving medicament
EP0661293A3 (en) * 1993-12-29 1999-05-19 Sanwa Kagaku Kenkyusho Co., Ltd. Protein having cell growth-stimulating and macrophage chemotactic actions, preparative method therefor and use thereof
GB9401689D0 (en) * 1994-01-28 1994-03-23 Univ Manchester Diagnosis and treatment of endocarditis
US6541447B1 (en) 1999-09-01 2003-04-01 B & M Healthcare Technologies, Inc. Wound healing composition and method for use thereof
JP4972412B2 (ja) * 2004-02-04 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション Ｅ．コリ発酵によるプラスミドｄｎａの大規模生産のための方法
KR101324578B1 (ko) 2006-02-03 2013-11-01 제이알 켐, 엘엘씨 구리 및 아연 조성물을 사용하는 노화 방지 치료법
US7687650B2 (en) 2006-02-03 2010-03-30 Jr Chem, Llc Chemical compositions and methods of making them
US7897800B2 (en) 2006-02-03 2011-03-01 Jr Chem, Llc Chemical compositions and methods of making them
CL2007002615A1 (es) * 2006-09-08 2008-04-18 Wyeth Corp Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto.
US7867522B2 (en) 2006-09-28 2011-01-11 Jr Chem, Llc Method of wound/burn healing using copper-zinc compositions
US8273791B2 (en) 2008-01-04 2012-09-25 Jr Chem, Llc Compositions, kits and regimens for the treatment of skin, especially décolletage
US20160184354A1 (en) 2009-01-23 2016-06-30 Jr Chem, Llc Rosacea treatments and kits for performing them
US8952057B2 (en) 2011-01-11 2015-02-10 Jr Chem, Llc Compositions for anorectal use and methods for treating anorectal disorders

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2944940A (en) * 1956-01-16 1960-07-12 Armour & Co Adrenocorticotrophin and zinc composition
NL268104A (ko) * 1961-08-10
SE393532B (sv) * 1974-05-02 1977-05-16 Draco Ab Sett att framstella en farmaceutisk zinkberedning for astadkommande av en smaklig, fordragbar, peroral zinklosning innehallande ett zinkkomplex
FR2269962B1 (ko) * 1974-05-06 1978-02-03 Anvar
JPS6019733B2 (ja) * 1977-06-10 1985-05-17 科研製薬株式会社 蛋白質性活性物質
JPS5424801A (en) * 1977-07-28 1979-02-24 Karupisu Shiyokuhin Kougiyou K Novel antibiotics cc3603 and process for preparing same
US4287184A (en) * 1977-11-23 1981-09-01 The Massachusetts General Hospital Process for healing wounds
AU517061B2 (en) * 1978-04-12 1981-07-09 Hoechst Aktiengesellschaft Alpha-amylase inhibitor
JPS5767591A (en) * 1980-10-11 1982-04-24 Kaken Pharmaceut Co Ltd Protein element substance and its preparation
US4436816A (en) * 1982-01-22 1984-03-13 Pitman-Moore, Inc. Cell growth promoting material
JPS58222026A (ja) * 1982-06-18 1983-12-23 Masahiro Yoshimura 抗ガン性物質
US4444760A (en) * 1983-06-17 1984-04-24 Merck & Co., Inc. Purification and characterization of a protein fibroblast growth factor

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ATE56729T1 (de) 1990-10-15

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