KR830006421A - 비글리코실화된 인터페론 및 이에 대한 용융된 암호 - Google Patents

비글리코실화된 인터페론 및 이에 대한 용융된 암호 Download PDF

Info

Publication number
KR830006421A
KR830006421A KR1019810002120A KR810002120A KR830006421A KR 830006421 A KR830006421 A KR 830006421A KR 1019810002120 A KR1019810002120 A KR 1019810002120A KR 810002120 A KR810002120 A KR 810002120A KR 830006421 A KR830006421 A KR 830006421A
Authority
KR
South Korea
Prior art keywords
interferon
gene
molten
unglycosylated
start position
Prior art date
Application number
KR1019810002120A
Other languages
English (en)
Inventor
피. 구아렌테 레오나드
프타슌 마크
엠. 로버츠 토마스
다니구치 타다쭈구
Original Assignee
히로시 안자이
캔서인스티튜트 어브 재피니스 파운데이숀 포캔서 리서치
프란시스 엠. 가브론
프레지던트 앤드 펠로우스 이브하버드 칼리지
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 히로시 안자이, 캔서인스티튜트 어브 재피니스 파운데이숀 포캔서 리서치, 프란시스 엠. 가브론, 프레지던트 앤드 펠로우스 이브하버드 칼리지 filed Critical 히로시 안자이
Publication of KR830006421A publication Critical patent/KR830006421A/ko

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

내용 없음

Description

비글리코실화된 인터페론 및 이에 대한 용윤된 암호
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
제1-4도는 본 발명의 비글리코실화된 인간섬유아세포 인터페론의 제조법의 여러 단계에 사용된 용융된 유전자의 도식임.
제3도와 4도는 인터페론에 대한 암호가 될 수 있는 본 발명의 용융된 유전자임.
제5-9도는 본 발명의 다른 인터페론제조에 사용된 용융된 유전자의 도식임.

Claims (8)

  1. 비글리코실화된 인간섬유 아세포 인터페론.
  2. 하기아미노산 서열은 지닌 청구범위 1의 인터페론:
  3. 제약학적으로 알맞는 무독성 매개물 및 효과적인 양의 청구범위 1에 따르는 단백질로 구성되는 제약학적 조성물.
  4. 가변성 촉진자, 2에서 14염기쌍, 번역시작위치 및 숙성한비글리코실화된 섬유 아세포 인터페론에 대한 암호가 되는 유전자로 구성되는 용융된 유전자.
  5. 가변성 촉진자, 2에서 14염기쌍, 번역시작위치 및 비글리코실화된 인간섬유 아세포 프리인터페론에 대한 암호가 되는 유전자로 구성되는 용융된 유전자.
  6. 비글리코실화된 인간백혈구 인터페론.
  7. 하기 아미노산서열을 지닌 청구범위 6의 인터페론:
  8. 가변성 촉진자, 2에서 14염기쌍, 번역시작위치 및 비글리코실화된 인간백혈구 프리인터페론에 대한 암호가 되는 유전자로 구성되는 용융된 유전자.
    참고사항 : 최초출원 내용에 의하여 공개하는 것임.
KR1019810002120A 1980-06-12 1981-06-12 비글리코실화된 인터페론 및 이에 대한 용융된 암호 KR830006421A (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15872180A 1980-06-12 1980-06-12
US158721 1980-06-12

Publications (1)

Publication Number Publication Date
KR830006421A true KR830006421A (ko) 1983-09-24

Family

ID=22569403

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019810002120A KR830006421A (ko) 1980-06-12 1981-06-12 비글리코실화된 인터페론 및 이에 대한 용융된 암호

Country Status (10)

Country Link
EP (1) EP0042246B1 (ko)
JP (1) JPS5777654A (ko)
KR (1) KR830006421A (ko)
AT (1) ATE25985T1 (ko)
AU (1) AU544218B2 (ko)
DE (1) DE3176011D1 (ko)
DK (1) DK254681A (ko)
ES (2) ES8300476A1 (ko)
IL (1) IL63063A0 (ko)
NO (1) NO811973L (ko)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK33181A (da) * 1980-02-06 1981-08-07 Searle & Co Fremgangsmaade til fremstilling af et interferonlignende protein
ES8302096A1 (es) 1980-04-03 1982-12-16 Biogen Nv Un metodo de producir un polipeptido que muestra una actividad immunologica obiologica de interferon de fibroblasto hu- mano.
CH651308A5 (de) * 1980-07-01 1985-09-13 Hoffmann La Roche Interferone und deren herstellung.
NZ198445A (en) * 1980-09-25 1984-05-31 Genentech Inc Production of human fibroblast interferon by recombinant dna technology
ZA816621B (en) * 1980-09-25 1982-09-29 Genentech Inc Microbial production of human fibroblast interferon
IL63327A (en) * 1981-07-16 1985-11-29 Yeda Res & Dev Production of interferon beta1 and alpha-phage recombinants for its production in host bacteria
IL66263A (en) * 1981-08-10 1985-12-31 Genex Corp Expression vectors for introducing a gene into a procaryotic organism
GB2108510B (en) * 1981-10-03 1984-12-12 Ciba Geigy Ag Dnas, recombinant dnas, hosts containing them, polypeptides and processes for the production thereof
US4973479A (en) * 1982-01-15 1990-11-27 Cetus Corporation Interferon-α61
US4975276A (en) * 1982-01-15 1990-12-04 Cetus Corporation Interferon-alpha 54
WO1983002460A1 (en) * 1982-01-15 1983-07-21 Cetus Corp Interferon-alpha 74
US5098703A (en) * 1982-01-15 1992-03-24 Cetus Corporation Interferon-alpha 76
US5827694A (en) * 1982-03-08 1998-10-27 Genentech, Inc. DNA encoding non-human animal interferons, vectors and hosts therefor, and recombinant production of IFN polypeptides
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
US6432677B1 (en) 1982-03-08 2002-08-13 Genentech, Inc. Non-human animal interferons
US5643566A (en) * 1982-09-23 1997-07-01 Cetus Corporation Formulation processes for lipophilic proteins
US4992271A (en) * 1982-09-23 1991-02-12 Cetus Corporation Formulation for lipophilic IL-2 proteins
US5702699A (en) * 1982-09-23 1997-12-30 Cetus Corporation Process for the recovery of lipophilic proteins
US4737462A (en) * 1982-10-19 1988-04-12 Cetus Corporation Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of interferon-β
US4588585A (en) * 1982-10-19 1986-05-13 Cetus Corporation Human recombinant cysteine depleted interferon-β muteins
US5831023A (en) * 1982-11-01 1998-11-03 Genentech, Inc. Recombinant animal interferon polypeptides
DE3247922A1 (de) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim Dna-sequenzen, deren herstellung, diese sequenzen enthaltende plasmide und deren verwendung zur synthese eukaryotischer genprodukte in prokaryoten
JPS59172496A (ja) * 1983-03-18 1984-09-29 Kyowa Hakko Kogyo Co Ltd モノクロ−ナル抗体
US4518584A (en) * 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins
NZ208309A (en) * 1983-06-01 1988-04-29 Hoffmann La Roche Recombinant leukocyte, fibroblast, or immune interferons (rifn-#a#, b or #g#) with one cys residue replaced
GB8412564D0 (en) * 1984-05-17 1984-06-20 Searle & Co Structure and properties
SU1364343A1 (ru) * 1984-07-13 1988-01-07 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Способ получени человеческого лейкоцитарного интерферона альфа-2
FI90990C (fi) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Rekombinantti-DNA-molekyyli, transformoitu isäntäorganismi ja menetelmä interferonin valmistamiseksi
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
WO1987004722A1 (en) * 1986-01-31 1987-08-13 Genetics Institute, Inc. Novel thrombolytic proteins
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
FR2598430B1 (fr) * 1986-05-06 1990-02-02 Roussel Uclaf Nouveaux vecteurs d'expression, leur procede d'obtention et leur application pour exprimer une proteine quelconque dans escherichia coli
US4839283A (en) * 1986-12-30 1989-06-13 Zymogenetics, Inc. Method of expressing alpha-1-antitrypsin in yeast

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL58765A (en) * 1979-11-21 1986-09-30 Yeda Res & Dev Process for the production of essentially pure messenger rna of human fibroblast interferon and process for the production of interferon beta
US4530901A (en) * 1980-01-08 1985-07-23 Biogen N.V. Recombinant DNA molecules and their use in producing human interferon-like polypeptides
IE54096B1 (en) * 1980-01-08 1989-06-21 Biogen Nv Dn sequences, recombinant dna molecules and processes for producing human interferon - like polypeptides
GB2068970B (en) * 1980-02-06 1983-06-22 Searle & Co Recombinant dna technique for the preparation of a protein resembling human interferon
DE3005897A1 (de) * 1980-02-16 1981-09-03 Hoechst Ag, 6000 Frankfurt Genprodukt eines hoeheren organismus aus einem dieses gen enhtaltenden mikroorganismus
DE3005843A1 (de) * 1980-02-16 1981-09-10 Hoechst Ag, 6000 Frankfurt Genprodukt eines hoeheren organismus aus einem dieses gen enthaltenden mikroorganismus
ES8302096A1 (es) * 1980-04-03 1982-12-16 Biogen Nv Un metodo de producir un polipeptido que muestra una actividad immunologica obiologica de interferon de fibroblasto hu- mano.
CH651308A5 (de) * 1980-07-01 1985-09-13 Hoffmann La Roche Interferone und deren herstellung.
NZ198445A (en) * 1980-09-25 1984-05-31 Genentech Inc Production of human fibroblast interferon by recombinant dna technology

Also Published As

Publication number Publication date
DK254681A (da) 1981-12-13
ES8303919A1 (es) 1983-02-16
ES502970A0 (es) 1982-11-01
EP0042246B1 (en) 1987-03-18
EP0042246A3 (en) 1982-10-13
ES8300476A1 (es) 1982-11-01
DE3176011D1 (en) 1987-04-23
IL63063A0 (en) 1981-09-13
ES512619A0 (es) 1983-02-16
AU544218B2 (en) 1985-05-23
AU7169481A (en) 1981-12-17
EP0042246A2 (en) 1981-12-23
NO811973L (no) 1981-12-14
ATE25985T1 (de) 1987-04-15
JPS5777654A (en) 1982-05-15

Similar Documents

Publication Publication Date Title
KR830006421A (ko) 비글리코실화된 인터페론 및 이에 대한 용융된 암호
ATE388963T1 (de) Homogene herstellungen von il-29
ES8207514A1 (es) Un procedimiento para la preparacion de interferon
FI874706A0 (fi) Menetelmä glykosyloimattoman yhdistelmäinterferoni- :n stabiilien, farmaseuttisten koostumusten valmistamiseksi
LV10507A (lv) Vektors sunas eritroproteins farmaceitiska kompozicija
DE69530374T2 (de) Blutbildendes fusionprotein mit il-3 varianten
DE69839812D1 (de) Analoge des menschlichen interferon-alpha mit niedriger toxizität
ES499261A0 (es) Procedimiento para la preparacion de un plasmido que codifica para la secuencia de aminoacidos del interferon de leuco- citos humanos.
DE3584198D1 (de) Neue genetische sequenzen, die durch sie codierten interferon-peptide vom typ i und diese sie produzierende organismen.
ZA924674B (en) Cysteine depleted il-6 mutein
NO173742C (no) Fremgangsmaate for fremstilling av et IFN- alpha-ekspresjonsplasmid og poly-peptid med IFN-aktivitet
NZ508816A (en) Bactericidal/permeability-increasing protein (BPI) deletion analogs
DE59108947D1 (de) Neues menschliches, rekombinantes interferon gamma
DE69227898D1 (de) Rekombinante hybride porinepitope
DE69106407T2 (de) Verfahren zur Herstellung von rekombinantem menschlichem gamma-Interferon ohne Cystein und ohne Methionin am N-Terminal.
IL88378A (en) Human interferon-gamma binding proteins their manufacture and pharmaceutical compositions comprising them
EP0072541A3 (en) Human leukocyte interferons, process for their microbial production, intermediates therefor and compositions containing them
NO983054L (no) Virale rekombinante pseudopartikler og anvendelse som vaksiner og som anti-tumormiddel
KATO et al. Fractionation of haemolymph protein of the silkworm, Bombyx mori L., by gel filtration and DEAE-cellulose column chromatography
KR890002399A (ko) 하이브리드 인터페론 및 재조합 dna기술을 이용한 그의 제조방법