JPS5777654A - Nonglycosylated interferon - Google Patents

Nonglycosylated interferon

Info

Publication number
JPS5777654A
JPS5777654A JP56089725A JP8972581A JPS5777654A JP S5777654 A JPS5777654 A JP S5777654A JP 56089725 A JP56089725 A JP 56089725A JP 8972581 A JP8972581 A JP 8972581A JP S5777654 A JPS5777654 A JP S5777654A
Authority
JP
Japan
Prior art keywords
plasmid
interferon
nonglycosylated
plg56
plg45
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP56089725A
Other languages
Japanese (ja)
Inventor
Pii Garente Renaado
Putashiyune Maaku
Emu Robaatsu Tomasu
Koreaki Taniguchi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Japanese Foundation for Cancer Research
Harvard College
Harvard University
Original Assignee
Japanese Foundation for Cancer Research
Harvard College
Harvard University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japanese Foundation for Cancer Research, Harvard College, Harvard University filed Critical Japanese Foundation for Cancer Research
Publication of JPS5777654A publication Critical patent/JPS5777654A/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/72—Expression systems using regulatory sequences derived from the lac-operon
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Polyesters Or Polycarbonates (AREA)

Abstract

NEW MATERIAL:A nonglycosylated interferon of human fibroblasts and nonglycosylated interferon of human leukocytes.
EXAMPLE: An interferon having an amino acid sequence of the formula.
USE: A powerful antiviral agent.
PROCESS: Four DNA fragments are linked to prepare a new plasmid pLG1. A BamHI linker having a length of 10 base pairs is inserted into the EcoRI site of the resultant pLG1 to give a plasmid pLG45. Coliform bacilli are then transformed by the resultant plasmid pLG45, and fragments selected from the growth in a culture medium containing amplicillin are linked by two DNA frahments to give a plasmid pLG56. Two plasmids pLG104 and pLG117 are obtained from the plasmid pLG56, and a plasmid 104R or 117R containing a reconstructing gene capable of coding the nonglycosylated preinterferon of human fibroblasts or matured interferon is obtained and used to produce the aimed interferon.
COPYRIGHT: (C)1982,JPO&Japio
JP56089725A 1980-06-12 1981-06-12 Nonglycosylated interferon Pending JPS5777654A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US15872180A 1980-06-12 1980-06-12

Publications (1)

Publication Number Publication Date
JPS5777654A true JPS5777654A (en) 1982-05-15

Family

ID=22569403

Family Applications (1)

Application Number Title Priority Date Filing Date
JP56089725A Pending JPS5777654A (en) 1980-06-12 1981-06-12 Nonglycosylated interferon

Country Status (10)

Country Link
EP (1) EP0042246B1 (en)
JP (1) JPS5777654A (en)
KR (1) KR830006421A (en)
AT (1) ATE25985T1 (en)
AU (1) AU544218B2 (en)
DE (1) DE3176011D1 (en)
DK (1) DK254681A (en)
ES (2) ES8300476A1 (en)
IL (1) IL63063A0 (en)
NO (1) NO811973L (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57140793A (en) * 1980-09-25 1982-08-31 Genentech Inc Microbial production of human fibroblast interferon
JPS5860998A (en) * 1981-07-16 1983-04-11 イエダ・リサ−チ・アンド・デベロツプメント・コンパニ−・リミテツド Production of interferon

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK33181A (en) * 1980-02-06 1981-08-07 Searle & Co PROCEDURE FOR THE PREPARATION OF AN INTERFERONOUS PROTEIN
ES8302096A1 (en) * 1980-04-03 1982-12-16 Biogen Nv DNA sequences, recombinant DNA molecules and processes for producing human fibroblast interferon.
CH651308A5 (en) * 1980-07-01 1985-09-13 Hoffmann La Roche INTERFERON AND THEIR PRODUCTION.
FR2490675B1 (en) * 1980-09-25 1985-11-15 Genentech Inc MICROBIAL PRODUCTION OF HUMAN FIBROPLASTER INTERFERON
IL66263A (en) * 1981-08-10 1985-12-31 Genex Corp Expression vectors for introducing a gene into a procaryotic organism
GB2108510B (en) * 1981-10-03 1984-12-12 Ciba Geigy Ag Dnas, recombinant dnas, hosts containing them, polypeptides and processes for the production thereof
US4973479A (en) * 1982-01-15 1990-11-27 Cetus Corporation Interferon-α61
US5098703A (en) * 1982-01-15 1992-03-24 Cetus Corporation Interferon-alpha 76
EP0098864A1 (en) * 1982-01-15 1984-01-25 Cetus Corporation Interferon-alpha 74
US4975276A (en) * 1982-01-15 1990-12-04 Cetus Corporation Interferon-alpha 54
US6432677B1 (en) 1982-03-08 2002-08-13 Genentech, Inc. Non-human animal interferons
IE54592B1 (en) * 1982-03-08 1989-12-06 Genentech Inc Anumal interferons, processes involved in their production, compositions containing them, dna sequences coding therefor and espression vehicles containing such sequences and cells transformed thereby
US5827694A (en) * 1982-03-08 1998-10-27 Genentech, Inc. DNA encoding non-human animal interferons, vectors and hosts therefor, and recombinant production of IFN polypeptides
US4992271A (en) * 1982-09-23 1991-02-12 Cetus Corporation Formulation for lipophilic IL-2 proteins
US5702699A (en) * 1982-09-23 1997-12-30 Cetus Corporation Process for the recovery of lipophilic proteins
US5643566A (en) * 1982-09-23 1997-07-01 Cetus Corporation Formulation processes for lipophilic proteins
US4588585A (en) * 1982-10-19 1986-05-13 Cetus Corporation Human recombinant cysteine depleted interferon-β muteins
US4737462A (en) * 1982-10-19 1988-04-12 Cetus Corporation Structural genes, plasmids and transformed cells for producing cysteine depleted muteins of interferon-β
US5831023A (en) * 1982-11-01 1998-11-03 Genentech, Inc. Recombinant animal interferon polypeptides
DE3247922A1 (en) * 1982-12-24 1984-06-28 Boehringer Ingelheim International GmbH, 6507 Ingelheim DNA SEQUENCES, THEIR PRODUCTION, PLASMIDES CONTAINING THESE SEQUENCES AND THE USE THEREOF FOR THE SYNTHESIS OF EUKARYOTIC GENE PRODUCTS IN PROKARYOTS
JPS59172496A (en) * 1983-03-18 1984-09-29 Kyowa Hakko Kogyo Co Ltd Monoclonal antibody
US4518584A (en) * 1983-04-15 1985-05-21 Cetus Corporation Human recombinant interleukin-2 muteins
DK265384A (en) * 1983-06-01 1984-12-02 Hoffmann La Roche POLYPEPTIDES WITH INTERFERON ACTIVITY
GB8412564D0 (en) * 1984-05-17 1984-06-20 Searle & Co Structure and properties
SU1364343A1 (en) * 1984-07-13 1988-01-07 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Method of producing manъs leukocytic interferon alfa-2
FI90990C (en) * 1984-12-18 1994-04-25 Boehringer Ingelheim Int Recombinant DNA molecule, transformed host organism, and method for producing interferon
JP2527454B2 (en) * 1986-01-31 1996-08-21 ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド New thrombolytic protein
US5258298A (en) * 1986-01-31 1993-11-02 Genetics Institute, Inc. Deletion and glycosylation mutant of human tissue plasminogen activator
US5837518A (en) * 1986-01-31 1998-11-17 Genetics Institute, Inc. Thrombolytic proteins
FR2598430B1 (en) * 1986-05-06 1990-02-02 Roussel Uclaf NOVEL EXPRESSION VECTORS, PROCESS FOR OBTAINING SAME, AND APPLICATION THEREOF FOR EXPRESSING ANY PROTEIN IN ESCHERICHIA COLI
US4839283A (en) * 1986-12-30 1989-06-13 Zymogenetics, Inc. Method of expressing alpha-1-antitrypsin in yeast

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5685296A (en) * 1979-11-21 1981-07-11 Yeda Res & Dev Isolation of mrna convertible to interferon and corresponding dna and production of interferonpolypeptide and high purity interferon
JPS56131522A (en) * 1980-02-16 1981-10-15 Hoechst Ag Genetic manipulation and product thereby
JPS56150100A (en) * 1980-01-08 1981-11-20 Biogen Nv Manufacture of dna order,recombination dna molecule and human interferon-like polypeptide
JPS56154499A (en) * 1980-04-03 1981-11-30 Biogen Nv Manufacture of dna order,recombination dna molecule and human fibroblast interferon-like polypeptide

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK170236B1 (en) * 1980-01-08 1995-07-10 Biogen Inc Recombinant DNA molecules, method for preparing an IFN-alpha type polypeptide and method for selecting DNA sequences encoding IFN-alpha type polypeptides
GB2068970B (en) * 1980-02-06 1983-06-22 Searle & Co Recombinant dna technique for the preparation of a protein resembling human interferon
DE3005897A1 (en) * 1980-02-16 1981-09-03 Hoechst Ag, 6000 Frankfurt GENERAL PRODUCT OF A HIGHER ORGANISM FROM A MICROORGANISM CONTAINING THIS GENE
CH651308A5 (en) * 1980-07-01 1985-09-13 Hoffmann La Roche INTERFERON AND THEIR PRODUCTION.
FR2490675B1 (en) * 1980-09-25 1985-11-15 Genentech Inc MICROBIAL PRODUCTION OF HUMAN FIBROPLASTER INTERFERON

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5685296A (en) * 1979-11-21 1981-07-11 Yeda Res & Dev Isolation of mrna convertible to interferon and corresponding dna and production of interferonpolypeptide and high purity interferon
JPS56150100A (en) * 1980-01-08 1981-11-20 Biogen Nv Manufacture of dna order,recombination dna molecule and human interferon-like polypeptide
JPS56131522A (en) * 1980-02-16 1981-10-15 Hoechst Ag Genetic manipulation and product thereby
JPS56154499A (en) * 1980-04-03 1981-11-30 Biogen Nv Manufacture of dna order,recombination dna molecule and human fibroblast interferon-like polypeptide

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57140793A (en) * 1980-09-25 1982-08-31 Genentech Inc Microbial production of human fibroblast interferon
JPS5860998A (en) * 1981-07-16 1983-04-11 イエダ・リサ−チ・アンド・デベロツプメント・コンパニ−・リミテツド Production of interferon

Also Published As

Publication number Publication date
EP0042246B1 (en) 1987-03-18
AU7169481A (en) 1981-12-17
ES8303919A1 (en) 1983-02-16
ES502970A0 (en) 1982-11-01
EP0042246A2 (en) 1981-12-23
DE3176011D1 (en) 1987-04-23
DK254681A (en) 1981-12-13
AU544218B2 (en) 1985-05-23
ATE25985T1 (en) 1987-04-15
IL63063A0 (en) 1981-09-13
EP0042246A3 (en) 1982-10-13
NO811973L (en) 1981-12-14
ES512619A0 (en) 1983-02-16
KR830006421A (en) 1983-09-24
ES8300476A1 (en) 1982-11-01

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