KR20240105664A - Cosmetic composition for scalp soothing comprising mixture of Camellia japonica pericarp extract and Cynanchum atratum extract as an active ingredient - Google Patents

Abstract

The present invention relates to a cosmetic composition for soothing the scalp containing camellia pericarp extract and white rice flower extract as active ingredients.
The composition according to the present invention is free from skin irritation and cytotoxicity and has excellent human safety, while suppressing histamine secretion from mast cells, promoting filaggrin production from keratinocytes, and suppressing the expression of pro-inflammatory cytokines from keratinocytes. It is useful as a cosmetic composition for soothing the scalp as it can relieve scalp itching, strengthen the scalp barrier, and improve scalp inflammation to soothe problematic scalp.

Description

Cosmetic composition for scalp soothing comprising mixture of Camellia japonica pericarp extract and Cynanchum atratum extract as an active ingredient}

The present invention relates to a composition for soothing the scalp containing camellia pericarp extract and white rice flower extract as active ingredients. More specifically, it relates to a cosmetic composition for soothing the scalp containing camellia pericarp extract and white rice flower extract as active ingredients, which can suppress scalp itchiness from external stimuli and soothe the scalp by strengthening the scalp barrier function.

The scalp of people living in modern society is exposed to various environmental pollution such as fine dust and yellow dust. In addition, cosmetic procedures such as dyeing, bleaching, perming, and drying cause irritation and damage to the scalp. In addition, scalp-related diseases are accelerating due to fatigue and stress caused by excessive work, irregular lifestyle habits, etc.

The scalp is a skin tissue that protects the head and has developed hair roots and sweat glands. The hair root of the scalp is the hair area within the skin and has sebaceous glands that secrete sebum to prevent moisture evaporation and bacterial infection, while sweat glands regulate body temperature by producing sweat. Proper sebum secretion in the skin protects the skin and gives it a beautiful glow, while excessively produced sebum causes chronic inflammation such as shiny skin, acne, and seborrheic dermatitis. Sebum secretion is known to be involved in hypersecretion of androgens, which are sex hormones, PPARγ (peroxidome proliferator activated receptor γ), which regulates the expression of retinoid and lipogenic genes, Wnt (Wingless) signal, and insulin. and is ultimately regulated by lipid synthesis or sebum cell differentiation mechanisms. In particular, if sebum is excessively produced on the scalp, there is a high possibility of developing seborrheic dermatitis. Seborrheic dermatitis is accompanied by erythema, scaling, and dandruff, creating an environment that causes itching and inflammation. Therefore, if seborrheic dermatitis is not treated properly, it can cause not only chronic scalp inflammatory disease but also hair loss.

Malasezia, which exists on the scalp, is reported to have a relatively large amount of lipolytic enzymes that decompose sebum on the scalp compared to other pathogenic fungi. Malassezia decomposes diglycerides, which are composed of triglycerides and esters, into monoglycerides and free fatty acids. Malassezia is the saturated glass produced in this process. We eat fatty acids (saturated free fatty acids) and leave the rest as metabolic residue. Among these metabolic residues, saturated free fatty acids are known to cause dandruff by destroying the scalp epithelial cell layer and abnormally promoting the formation of the stratum corneum. This process causes damage to the scalp's barrier function, making it difficult to maintain homeostasis of the scalp barrier, making it sensitive to external stimuli, and disrupting the balance of oil and moisture in the scalp. Oleic acid, an unsaturated fatty acid, stimulates mast cells in the scalp to secrete histamine and protease, causing itching and inflammation, and physical stimulation such as scratching due to itching may lead to secondary bacterial infection. . Additionally, studies have shown that palmitic acid, a saturated fatty acid, promotes lipid synthesis in sebum cells and causes inflammatory reactions.

The general characteristics of problematic scalp appear in the stratum corneum, the superficial layer of the skin. The stratum corneum serves as a barrier against pathogenic invasion by microorganisms, toxins, oxidants, and ultraviolet rays. At the same time, the most important role of the stratum corneum is to act as a barrier against moisture loss. The loss of this most basic function means loss of function as an epidermal permeability barrier. An essential factor that helps maintain moisture balance within the stratum corneum is natural moisturizing factor (NMF), which is produced by the breakdown of filaggrin during the differentiation of skin keratinocytes. Filaggrin is a substance that helps the keratin of keratinocytes to come together. It is a protein that forms a skin barrier and holds the structure firmly together. Therefore, strengthening this barrier function has become a target for the development of scalp soothing materials that can protect the scalp from external stimuli and prevent scalp inflammation.

Currently, antifungal agents, steroids, and immunomodulatory substances are used to treat scalp inflammation and itching, but there are few treatments that are safe, effective, and can be used for a long time, and market demand is increasing. Therefore, attempts are being made to improve or alleviate scalp problems by suppressing inflammation and itching caused by internal and external stimuli or strengthening the skin barrier function.

Camellia japonica L. is an evergreen tree belonging to the Camellia family. Numerous horticultural varieties have been created, and most of them have been used as ornamental trees or as camellia oil extracted from seeds. Camellia flowers contain leucoanthocyanin and anthocyanin, which have excellent physiological activity, and the fruit contains fatty oil, camellin, and thachysaponin. Camellia oil, which has been widely used as hair oil since ancient times, contains about 80% oleic acid, an omega-9, and also contains polyphenols, vitamins A, C, D, E and other antioxidants. It is known that they are doing it. These secondary metabolites are known to have effects such as cardiotonic effect, hemostasis, hemostasis, live fish, and small intestine.

Regarding the efficacy of camellia tree, Republic of Korea Patent No. 10-1672431 discloses the cardiovascular disease prevention effect of camellia tree extract, and Republic of Korea Patent No. 10-1408019 discloses the antioxidant and whitening effect of camellia fruit extract, Republic of Korea No. 10-1452320 discloses the hair loss improvement effect of camellia oil, but nothing has yet been revealed about the scalp improvement effects of Camellia japonica pericarp extract, such as relieving scalp itchiness and strengthening the scalp barrier.

White rice flower ( Cynanchum atratum Bunge) is a perennial herbaceous plant of the genus Cynanchum in the family Cucurbitaceae. It blooms dark purple flowers and is used for ornamental and medicinal purposes. The root has an antipyretic effect and is known to be effective in pulmonary tuberculosis, premature fever, blood fever, ischemic fever, hematemesis, nephritis, and urinary tract infections. Biphenylneolignan and pregnane glycosides found in the roots of white rice flowers are reported to have anti-inflammatory, anti-atopic, anti-fungal, and melanin production-inhibiting effects.

Regarding the efficacy of white rice flowers, Republic of Korea Patent No. 10-1285234 discloses the efficacy of white rice flower extract in preventing and treating arthritis, and Republic of Korea Patent No. 10-1047588 discloses the whitening effect of white rice flower extract, and Korea Patent No. 10- No. 1493461 discloses the tuberculosis treatment effect of white rice flower extract, and Republic of Korea Patent No. 10-1179966 discloses the anti-inflammatory effect of a complex extract containing white rice flower extract, but there is still no evidence for white rice flower extract, such as suppressing scalp itching and strengthening the scalp barrier. Nothing has been revealed about its effectiveness in improving the scalp.

Accordingly, the present inventors conducted research to find a material for improving problematic scalp by suppressing itching of the scalp caused by external stimuli and strengthening the barrier function of the scalp, and found that a composite extract of camellia pericarp extract and white rice flower extract is superior to any other material. It was discovered that it has excellent efficacy in improving problematic scalp compared to other products, and thus the present invention was completed.

Republic of Korea Patent No. 10-1672431 (announced on November 4, 2016) Republic of Korea Patent No. 10-1408019 (announced on June 18, 2014) Republic of Korea Patent No. 10-1452320 (announced on October 22, 2014) Republic of Korea Patent No. 10-1285234 (announced on July 11, 2013) Republic of Korea Patent No. 10-1047588 (announced on July 8, 2011) Republic of Korea Patent No. 10-1493461 (announced on February 16, 2015) Republic of Korea Patent No. 10-1179966 (announced on August 30, 2012)

Inui et al., J Dermatol Sci., 61(1):1-6, 2011 Jin et al., Bioorg Med Chem Lett. 2018, 28(7):1252-1256. Choi et al., Biomed Pharmacother. 2017, 90:321-327. Chen at al., Evid Based Complement Alternat Med. 2020, 2020:7259686. Xin et al., Postharvest Biol Technol. 2019, 157:110961

The purpose of the present invention is to provide a cosmetic composition for soothing the scalp by relieving scalp itching, strengthening the function of the scalp barrier, and improving scalp inflammation, comprising camellia pericarp extract and white rice flower extract as active ingredients.

To achieve the above object, the present invention provides a cosmetic composition for soothing the scalp by alleviating scalp itching, strengthening the function of the scalp barrier, and improving scalp inflammation, comprising camellia pericarp extract and white rice flower extract as active ingredients. .

The composition containing camellia pericarp extract and white rice flower extract of the present invention as active ingredients has no skin irritation or cytotoxicity, so it has excellent human safety, suppresses histamine secretion from mast cells, relieves itching, and strengthens the scalp barrier function. Since it is very effective in maintaining the homeostasis of the scalp barrier and inhibiting the production of pro-inflammatory cytokines that cause scalp inflammation, it can be usefully used as a cosmetic composition for soothing the scalp.

Figure 1 is a diagram showing the inhibitory effect of histamine secretion in mast cells of each extract of camellia pericarp and white rice flower extract and the combined extract of camellia pericarp extract and white rice flower extract according to an embodiment of the present invention.
Figure 2 is a graph showing the effect of promoting filaggrin mRNA expression by a complex extract of camellia pericarp extract and white rice flower extract according to an embodiment of the present invention.
Figure 3 is a graph showing the inhibition of pro-inflammatory cytokine expression in keratinocytes by a complex extract of camellia pericarp extract and white rice flower extract according to an embodiment of the present invention.

The present invention relates to a cosmetic composition for soothing the scalp containing camellia pericarp extract and white rice flower extract as active ingredients. The complex extract of camellia pericarp extract and white rice flower extract according to the present invention has no skin irritation or cytotoxicity, so it has excellent human safety, suppresses histamine secretion from mast cells, promotes the production of filaggrin in keratinocytes, and promotes the production of filaggrin in keratinocytes. By suppressing the expression of pro-inflammatory cytokines, it can relieve scalp itching, strengthen the scalp barrier, and improve scalp inflammation to soothe problematic scalp.

More specifically, the present invention provides a cosmetic composition for soothing the scalp by alleviating scalp itching, strengthening the function of the scalp barrier, and/or improving scalp inflammation, comprising camellia pericarp extract and white rice flower extract as active ingredients.

The term " Camellia japonica L." in the present invention is an evergreen tree belonging to the Camellia family. Numerous horticultural varieties have been created and most of them are cultivated as ornamental trees or used for camellia oil squeezed from seeds.

The term "pericarp" in the present invention refers to the remaining part of the fruit excluding the seeds, and in the present invention, it refers to all parts remaining after removing the seeds from the fruit of the camellia tree.

The term " Cynanchum atratum Bunge" in the present invention is a perennial herb belonging to the genus Cynanchum atratum of the Cynanchum family. In producing the extract described below, various parts of the white rice flower, such as leaves, roots, stems, flowers, and above-ground parts, can be used, and the above-ground part is preferably used.

In the present invention, the "extract" refers to a liquid component obtained by immersing each or a mixture of camellia pericarp and white rice flower or a mixture thereof in various solvents and then extracting and fractionating them at room temperature or at a heated state for a certain period of time for the purpose of the present invention. , refers to the result, such as solid content, obtained by removing the solvent from the liquid component. In addition, in addition to the above results, it can be comprehensively interpreted to include dilutions of the results, concentrates thereof, crude preparations, purifications, etc. of the above results.

The solvent may be water or various organic solvents, and is not particularly limited as long as an extract having the effect of relieving scalp itching, strengthening the scalp barrier, and/or improving scalp inflammation can be obtained. More specifically, the solvent may be water, a polar solvent, or a non-polar solvent, and preferably may be water, a lower alcohol having 1 to 4 carbon atoms (ethanol, methanol, propanol, or butanol, etc.), or a mixed solvent thereof. and, most preferably, ethanol can be used.

In addition, the method for obtaining the extract is not particularly limited, as long as it is possible to obtain an extract that has the effect of alleviating scalp itching, strengthening the scalp barrier, and inhibiting the production of pro-inflammatory cytokines in the scalp. Extraction methods include cold needle extraction, ultrasonic extraction, or reflux cooling extraction, and it is preferable to use the ultrasonic extraction method.

The ultrasonic extraction is a method of extracting useful components from various natural materials at room temperature using physical force rather than chemical force using a vibrating element with a frequency of more than 20,000 times per second. Solvent extraction, vegetable oil extraction, or steam distillation extraction Unlike other methods, it has the advantage of being able to extract high concentrations of active ingredients contained in natural materials at room temperature without destroying them.

As a specific example, the ultrasonic extraction can be performed on dried camellia bark powder, white rice flower powder, or a mixture thereof at room temperature for 24 to 72 hours using a 60% to 80% ethanol solution. there is. Preferably, it is carried out at room temperature for 36 to 60 hours using a 65% to 75% ethanol solution, and more preferably, it is carried out at room temperature for 48 hours using a 70% ethanol solution. If the value exceeds the lower limit of the above value, the active ingredient of the present invention contained in the camellia pericarp and white rice flower is not sufficiently extracted, and if the upper limit is exceeded, there is no significant difference in the amount of the active ingredient extracted, impurities other than the active ingredient are extracted, and process efficiency is increased. This decreases.

The obtained Camellia pericarp extract, White rice flower extract, and their complex extracts are not limited thereto, but may be concentrated under reduced pressure using a vacuum concentrator or vacuum rotary evaporator. Additionally, after concentration under reduced pressure, it may be dried through, but not limited to, freeze-drying, reduced-pressure drying, vacuum drying, boiling drying, or spray drying.

The composition of the present invention may contain the camellia pericarp extract and the white rice flower extract by mixing each at a certain weight ratio, or the camellia pericarp and the white rice flower extract may be mixed and extracted at a constant weight ratio, and this is also referred to as a “composite extract” for convenience. The "constant weight ratio" refers to a weight ratio of Camellia pericarp extract or Camellia pericarp and White rice flower extract or White rice flower of 0.8 to 1.2: 0.8 to 1.2, preferably 0.9 to 1.1: 0.9 to 1.1, most preferably 1: 1. is the weight ratio of The complex extract prepared at the above-mentioned constant weight ratio is significantly superior to the individual extracts in the scalp itching alleviation effect, scalp barrier strengthening effect, and production inhibition effect of pro-inflammatory cytokines that cause scalp inflammation, which are described below, compared to the individual extracts. In this case, there is a problem that the above effect is reduced.

The complex extract of the camellia pericarp extract and the white rice flower extract according to the present invention improves and treats problem scalps such as itchiness on the scalp, imbalance of oil and moisture in the scalp due to weakening of the scalp barrier, and increased inflammation of the scalp to a normal scalp. It is effective for At this time, the process of turning or treating a problematic scalp into a normal scalp, or improving or treating a problematic scalp into a normal scalp, is called “scalp soothing.”

As a specific example, the composite extract of Camellia pericarp extract and White rice flower extract according to the present invention has an excellent effect of suppressing histamine secretion from mast cells and is therefore effective in alleviating or suppressing scalp itching.

The above-mentioned “alleviating scalp itching” or “suppressing scalp itching” refers to stimulation of mast cells by metabolic residues caused by Malasezia present in the scalp, sebum or dandruff excessively produced in the scalp, or seborrheic dermatitis. By reducing the physiological phenomenon of scratching the scalp, it not only prevents wounds caused by scalp scratching, but also prevents secondary bacterial infection in wounds caused by scalp scratching.

As another specific example, the complex extract of camellia pericarp extract and white rice flower extract according to the present invention is effective in strengthening the scalp barrier because it promotes the production of filaggrin protein in the keratinocytes of the scalp.

The "scalp barrier" refers to the role of protecting the scalp from invasion of external pollutants such as external microorganisms, toxic substances, oxidants, ultraviolet rays, etc., and preventing the loss of moisture within the scalp. This strengthening of the scalp barrier is achieved by strengthening the scalp's dead skin cells. This can be achieved by stimulating the production of filaggrin protein within cells.

As another specific example, the complex extract of Camellia pericarp extract and White rice flower extract according to the present invention has an excellent effect of suppressing the expression of pro-inflammatory cytokines in keratinocytes of the scalp, and is therefore effective in suppressing or improving scalp inflammation.

The term “inhibition of scalp inflammation” or “improvement of scalp inflammation” refers to reducing the in vivo reaction of the scalp caused by excessively produced sebum or dandruff on the scalp, or infection of a wound due to itching of the scalp.

Therefore, the composition containing the camellia pericarp extract and the white rice flower extract according to the present invention as active ingredients is excellent in suppressing histamine secretion, strengthening the barrier function, and suppressing the production of pro-inflammatory cytokines, thereby soothing the scalp. It is useful as a composition for

In the composition of the present invention, the active ingredient of the complex extract of Camellia pericarp extract and White rice flower extract is 0.0001 to 10.0% by weight, preferably 0.001 to 10.0% by weight, more preferably 0.01 to 10.0% by weight, based on the total weight of the composition. It may include weight%, more preferably 0.1 to 10.0 weight. If the above content of the complex extract is less than 0.0001% by weight, the effect of relieving inflammation and itchiness of the scalp and strengthening the scalp barrier is too weak to be useful for soothing the scalp, and if it exceeds 10.0% by weight, the effect increases as the content increases. There is a problem that the increase is insufficient and the stability of the formulation is not secured.

The cosmetic composition of the present invention contains ingredients commonly used in cosmetic compositions in addition to the active ingredients, Camellia pericarp extract and White rice flower extract, such as antioxidants, solubilizers, stabilizers, vitamins, pigments, and fragrances. It may contain conventional auxiliaries and carriers.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing, It may be formulated as oil, powder foundation, emulsion foundation, wax foundation, spray, etc., but is not limited thereto. In addition, it can be manufactured in the form of nutritional cream, astringent lotion, softening lotion, lotion, essence, shampoo, nutritional gel, or massage cream.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivatives, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide are used as carrier ingredients. It can be.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the formulation is a spray, chlorofluorohydrocarbon and propane may be used as carrier ingredients. /May contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 , 3-butyl glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, the carrier ingredients include water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester, and microcrystals. Cellulose, aluminum metahydroxide, bentonite, agar, or tragacanth can be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, and fatty acid amide. Ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

In particular, the cosmetic composition of the present invention can be provided as a hair care cosmetic composition that relieves inflammation and itching of the scalp by applying it to the scalp and soothes the scalp by strengthening the scalp barrier.

The cosmetic composition for hair care includes, for example, hair shampoo, hair rinse, hair conditioner, hair treatment, hair tonic, scalp treatment, hair lotion, hair cream, hair mist, hair ampoule, hair pack, hair essence, hair There are oils, scalp scalers, hair nourishing lotions, and hair dyes.

Meanwhile, the active ingredient of the present invention, a complex extract of camellia pericarp extract and white rice flower extract, was found to be a substance harmless to the human body as a result of a cumulative skin irritation test. Therefore, the composite extract of the camellia pericarp extract and the white rice flower extract of the present invention has almost no toxicity and side effects, so it can be used safely even for long-term use, and can be safely used in cosmetics.

Hereinafter, to aid understanding of the present invention, it will be described in detail through examples. However, the embodiments according to the present invention may be modified into various other forms, and the scope of the present invention should not be construed as being limited to the following embodiments. Embodiments of the present invention are provided to more completely explain the present invention to those skilled in the art.

Example 1: Selection of natural products with scalp soothing and improving effects

Based on the results of previous research on improving problematic scalp, camellia pericarp and white rice flower were selected, and the extracts obtained from them were verified for their efficacy in suppressing inflammatory factor production, histamine secretion, and strengthening barrier function.

1-1. Preparation of Camellia pericarp extract

The seeds were separated from the camellia fruit, and the remaining skin was washed thoroughly to completely remove foreign substances and impurities, dried in the shade at 20°C to 35°C, and then ground to a particle size of 1 mm or less. Afterwards, 1 kg of the pulverized camellia rind powder was immersed in 70% ethanol solvent and subjected to ultrasonic extraction for 48 hours, and the obtained extract was filtered using filter paper (Advantec, No. 2). The filtrate was concentrated under reduced pressure to remove the ethanol solvent and then freeze-dried to prepare camellia rind extract powder.

1-2. Preparation of white rice flower extract

The aerial parts of white rice flowers were thoroughly washed to completely remove foreign substances and impurities, dried in the shade at 20°C to 35°C, and then ground to a particle size of 1 mm or less. Thereafter, 1 kg of the pulverized white rice flower powder was immersed in 70% ethanol solvent and subjected to ultrasonic extraction for 48 hours, and the obtained extract was filtered using filter paper (Advantec, No. 2). The filtrate was concentrated under reduced pressure to remove the ethanol solvent and then freeze-dried to prepare white rice flower extract powder.

1-3. Manufacture of complex extract containing camellia rind extract and white rice flower extract

A composite extract was prepared by mixing the Camellia pericarp extract of 1-1 and the above-mentioned aerial part of white rice flower extract of 1-2 at a weight ratio of 1:1.

Example 2: In vitro ( in vitro ) Measurement of histamine secretion inhibition effect of complex extract

To determine the effect of the complex extract on histamine, which induces scalp itching, the ability to inhibit histamine release was measured.

Specifically, MC/9 cells (mouse mast cell line, ATCC), which are mouse mast cells, were cultured in DMEM medium containing 10% Rat T-STIM, 10% FBS, and 0.05mM 2-mercaptoethanol. MC/9 cells were recovered, suspended in Tyrode HEPES buffer, and distributed at 1 × 10 ⁶ cells per well in a 24-well plate. The composite extract, white rice flower extract, and camellia pericarp extract were each pretreated for 1 hour, then treated with oleic acid at a concentration of 100 μM and incubated again for 3 hours. After 3 hours of incubation, samples were prepared by centrifuging and collecting only the cell culture supernatant. The amount of histamine released was measured using a histamine measurement kit (Histamine ELISA kit, Enzo, USA). The detailed implementation method was performed according to Enzo's kit instructions. The level of liberated histamine was converted to the amount compared to the histamine standard curve, and the results are shown in Figure 1.

As shown in Figure 1, histamine release from mast cells increased by oleic acid was suppressed by about 7.3% when treated with 100 ppm of white rice flower extract alone, and by about 63.2% when treated with 100 ppm of Camellia pericarp extract alone. It was inhibited, and when the complex extract was treated at 100 ppm, the inhibition effect was about 82.5%. Therefore, it was found that the composite extract showed a very superior efficacy in inhibiting histamine release from mast cells compared to the individual extracts.

Example 3: In vitro ( in vitro ) Inhibitory effect on the production of pro-inflammatory cytokines derived from sodium lauryl sulfate of the complex extract.

To determine the effect of the complex extract on pro-inflammatory cytokines that cause scalp inflammation, the ability to inhibit pro-inflammatory cytokine production was measured.

Specifically, human epidermal keratinocytes (HEKn, Thermo Fisher, USA) were cultured in EpiLife Medium (Thermo Fisher, USA) containing Human Keratinocyte Growth Supplement (HKGS, Thermo Fisher, USA). HEKn were seeded in a 24-well plate at 2 × 10 ⁴ cells per well and cultured until more than 70% of the wells were covered. HEKn, which was exchanged for EpiLife Medium without HKGS, was pretreated with the complex extract for 1 hour, then treated with 100 μM sodium lauryl sulfate (SLS) and cultured again for 24 hours. After 24 hours of incubation, cell culture fluid was collected and samples were prepared. The amount of TNF-α and IL-1β produced from sodium lauryl sulfate treatment was measured using an ELISA kit for measuring TNF-a and IL-1b (R&D Systems, USA). The detailed implementation method was carried out according to the kit instructions from R&D Systems. The levels of free TNF-α and IL-1β were converted to each standard curve, and the results are shown in Figure 2.

As shown in Figure 2, the production of pro-inflammatory cytokines (TNF-α, IL-1β) in HEKn, which was increased by sodium lauryl sulfate, was suppressed in a concentration-dependent manner by the complex extract, when the complex extract was treated at 100 ppm. When TNF-α is about 76.3% and IL-1β is about 75% It showed a certain level of inhibitory efficacy.

Example 4: In vitro ( in vitro ) Effect of complex extract on promoting filaggrin expression

To determine the effect of the complex extract on strengthening scalp barrier function, the ability to promote filaggrin production was measured.

Specifically, human epidermal keratinocytes (HEKn, Thermo Fisher, USA), which are human keratinocytes, were cultured in EpiLife Medium (Thermo Fisher, USA) containing Human Keratinocyte Growth Supplement (HKGS, Thermo Fisher, USA). HEKn were seeded in a 6-well plate at 2 × 10 ⁵ cells per well and cultured until more than 70% of the wells were covered. HEKn, which had been exchanged for EpiLife Medium without HKGS, was treated with the complex extract at different concentrations and cultured again for 60 hours. As a positive control, 1.5mM CaCl ₂ was treated. After culturing for 60 hours, cells were recovered and tRNA was extracted using the RNeasy kit (Qiagen, Germany). The extracted tRNA was synthesized into cDNA using AccuPower ^® RT PreMix & Master Mix (Bioneer, Korea). The synthesized cDNA was used to measure the mRNA expression level of filaggrin using quantitative real time PCR (Applied Biosystems, USA). The mRNA expression level of filaggrin was converted to fold by comparing it with the expression of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), a housekeeping gene, and the results are shown in Figure 2. It is shown in 3.

As shown in Figure 3, the production of filaggrin was promoted by about 3.7 fold when treated with 100 ppm of the complex extract compared to the untreated group, which was the negative control, and showed almost similar efficacy compared to CaCl ₂ , which was the positive control. It was.

Example 5: Safety confirmation test of complex extract on human skin

A skin safety verification experiment was performed to confirm whether the complex extract according to the present invention was safe for human skin. For this purpose, a skin cumulative irritation test was conducted.

A nutritious cream containing 0.1%, 0.5%, and 1% of the complex extract of Example 1, respectively, was prepared. Specifically, purified water, triethanolamine, and propylene glycol as aqueous phase were heated to 70°C and dissolved, and then beeswax, liquid paraffin, oil phase ingredients, emulsifier, and preservative were added and emulsified. After emulsification was completed, the solution was cooled to 45°C, 0.1%, 0.5%, and 1% of the composite extract were added and dispersed, respectively, and then cooled to 30°C. As the content of the complex extract was increased to 0.1%, 0.5%, and 1%, the nutritional cream was prepared by reducing the liquid paraffin content to 9.91%, 9.51%, and 9.01%, respectively.

Using the nutritive cream prepared as above, 30 healthy adults were applied to the upper forearm area 9 times a cumulative patch for 24 hours every other day to measure whether the complex extract irritates the skin.

The patch method used a Finn chamber (Epitest Ltd, Finland). 15 ㎕ of each skin external preparation was added dropwise to the chamber, and then a patch was applied. The degree of reaction on the skin each time was scored using the following [Equation 2], and the results are shown in Table 1 below.

[Equation 2]

Average reactivity = [[(response index × reactivity)/(total number of subjects × highest score (4 points))] × 100] ÷ number of tests (9)

In reactivity, ± is given a score of 1 point, + is 2 points, and ++ is given a score of 4 points, and when the average reactivity is less than 3, the composition is judged to be safe.

test substance Number of subjects who responded (people) average
Responsiveness 1 week 2 weeks 3 weeks Primary Secondary 3rd 4th 5th 6th 7th 8th 9th ±/+/++ ±/+/++ ±/+/++ ±/+/++ ±/+/++ ±/+/++ ±/+/++ ±/+/++ ±/+/++
control group
-/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- 0.00 complex extract
(0.1%) -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- 0.00 complex extract
(0.5%) -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- 0.00 complex extract
(One%) -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- -/-/- 0.00 Number of people examined 30 30 30 30 30 30 30 30 30 0.00

As shown in Table 1, in test groups 1, 2, and 3, the number of people corresponding to ±, +, and ++ was 0, and the average response was also 0.00. Since the test results showed an average reactivity of 3 or less, the camellia pericarp extract according to the present invention was determined to be a safe material for human skin without showing a clear cumulative irritation pattern.

The composition of the present invention can be prepared as a formulation example as follows, but is not limited thereto.

Formulation Example 1: Cosmetic formulation

1-1: Soft lotion

As shown in Table 2 below, a softening lotion containing a complex extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Complex extract of Example 1 0.1 glycerin 3.0 butylene glycol 2.0 propylene glycol 2.0 Carboxy vinyl polymer 0.1 ethanol 10.0 Triethanolamine 0.1 Preservatives, trace colors, trace fragrances and trace amounts of purified water 82.7 sum 100.0

1-2: Massage Cream

As shown in Table 3 below, a massage cream containing the complex extract of Example 1 as an active ingredient was prepared according to a conventional method.

ingredient weight% Complex extract of Example 1 0.1 beeswax 10.0 Polysorbate 60 1.5 Sorbitan sesquioleate 0.8 liquid paraffin 40.0 squalane 5.0 Caprylic/Capric Triglyceride 4.0 glycerin 5.0 butylene glycol 3.0 propylene glycol 3.0 Triethanolamine 0.2 Preservatives, trace pigments, trace fragrances and trace purified water 27.4 sum 100.0

1-3: Pack

As shown in Table 4 below, a pack containing the complex extract of Example 1 as an active ingredient was prepared according to a conventional method.

ingredient weight% Complex extract of Example 1 0.1 polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 allantoin 0.1 ethanol 5.0 Nonylphenyl ether 0.3 Preservatives, trace pigments, trace fragrances and trace purified water 81.3 sum 100.0

1-4: Shampoo

As shown in Table 5 below, shampoo containing the complex extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Complex extract of Example 1 0.1 Ammonum Lauryl Sulfate 17 Ammonium Laureth Sulfate 29 Cocamidopropyl Betaine 2 Cocamide DEA 2 Citric Acid 0.2 Polyquaternium-7 3 glycerin One sodium chloride 1.5 Fragrance, preservatives, purified water 44.2 sum 100.0

1-5: Rinse

As shown in Table 6 below, a rinse containing the complex extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Complex extract of Example 1 0.1 Guahydroxypropyltrimonium chloride 0.5 steartrimonium chloride 4.0 Cetearyl alcohol 5.0 beeswax 2.0 Dimethicone 3.0 Citric Acid 0.01 Preservatives, minor flavorings and purified water 85.39 sum 100.0

1-6: Pack

As shown in Table 7 below, a pack containing the complex extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Complex extract of Example 1 0.1 polyvinyl alcohol 13.0 Sodium Carboxymethyl Cellulose 0.2 allantoin 0.1 ethanol 5.0 Nonylphenyl ether 0.3 Preservatives, trace pigments, trace fragrances and trace purified water 81.3 sum 100.0

1-7: Hair Serum

As shown in Table 8 below, hair serum containing the complex extract as an active ingredient was prepared according to a conventional method.

ingredient weight% Complex extract of Example 1 0.1 menthol 0.1 Polysorbate 20 0.5 ethanol 5.0 panthenol 0.1 Niacinamide 0.3 Pyridoxine HCL 0.1 Dipropylene glycol 2.0 Preservatives, trace pigments, trace fragrances and trace purified water 91.8 sum 100.0

Formulation Example 2: Pharmaceutical formulation

2-1: Preparation of gel

ingredient weight% Complex extract of Example 1 0.1 Butylene glycol 2.00 glycerin 1.00 Disodium EDTA 0.02 PEG-60 Hydrogenated Castor Oil 1.00 Cocamidopropyl Betaine 4.00 Lauryl Glucoside 6.00 Polysorbate 20 1.00 Sodium Benzoate 0.40 caprylic glycol 0.20 Sodium Lactate 0.50 Hydroxyethylcellulose 0.7 Purified water 83.08 sum 100.0

2-2: Preparation of ointment

ingredient weight% Complex extract of Example 1 0.1 Diethanolamine 1.5 Polyvinylphtolidone 5.0 propylene glycol 30.0 Purified water 63.4 sum 100.0

Claims (6)

A cosmetic composition for soothing the scalp by relieving scalp itching, strengthening the function of the scalp barrier, or improving scalp inflammation, comprising camellia pericarp extract and white rice flower extract as active ingredients. The cosmetic composition for soothing the scalp according to claim 1, wherein the camellia pericarp is a portion remaining after seeds are removed from the fruit of the camellia tree. The cosmetic composition for soothing the scalp according to claim 1, wherein the white rice flower extract is an extract of the aerial part of white rice flowers. The cosmetic composition for soothing the scalp according to claim 1, wherein the camellia pericarp extract and the white rice flower extract are contained in a weight ratio of 0.8 to 1.2:0.8 to 1.2. The cosmetic composition for soothing the scalp according to claim 1, wherein the extract is extracted with water, C1 to C4 lower alcohol, or a mixture thereof. The method of claim 1, wherein the cosmetic composition includes hair shampoo, hair rinse, hair conditioner, hair treatment, hair tonic, scalp treatment, hair lotion, hair cream, hair mist, hair ampoule, hair pack, hair essence, and hair oil. A cosmetic composition for soothing the scalp, characterized in that it is a formulation selected from the group consisting of scalp scaler, hair nourishing lotion, and hair dye.