KR20240063265A - Manufacturing method of oriental medicine pills containing lactic acid bacteria fermented kelp extract - Google Patents

Abstract

The present invention relates to a method of manufacturing an herbal medicine pill containing lactic acid bacteria-fermented kelp extract. It provides a lactic acid bacteria-fermented kelp extract that is beneficial to health by fermenting lactic acid bacteria using kelp, a seaweed abundant in the sea, and provides an herbal medicine pill that is easy to consume and has an herbal medicine effect. In order to manufacture it into rings, the prepared kelp is washed, desalted, and then put into a crusher through a crushing process to obtain crushed kelp (S1); Putting the pulverized kelp into a heating container, adding purified water 15 to 17 times the volume of the kelp, and performing hot water extraction while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract (S2); Adding Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, to the hot water extract and stirring for 4 to 4.5 hours at pH 4.5, 60 to 63°C to obtain an enzymatic kelp extract (S3); After growing the lactic acid bacteria inoculated on a Lactobacilli MRS agar plate by culturing at 37-39°C for 2 days, the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and cultured at 37-39°C for 18-22 hours. Creating a pre-culture solution and then cultivating the pre-culture solution and the enzymatic kelp extract with shaking to obtain a kelp lactic acid bacteria fermentation product (S4); Filtering the cultured kelp lactic acid bacteria fermented product through a mesh sieve of 70 to 80 mesh, and then sterilizing the filtered kelp lactic acid bacteria fermented product at 120 to 125°C for 15 to 30 minutes under high temperature and pressure (S5); Concentrating the high-temperature autoclaved fermented kelp lactic acid bacteria under reduced pressure to 19 to 20 brix to obtain a concentrate, then transferring the concentrate to a tray and freezing it at -20 to -22°C for 24 to 26 hours (S6); Inserting the frozen fermented kelp lactic acid bacteria into a freeze dryer and drying it for 5 to 6 days (S7); Step (S8) of pulverizing the dried fermented kelp lactic acid bacteria in a grinder and then passing it through a mesh sieve of 58 to 60 mesh to obtain fermented kelp lactic acid bacteria powder (S8); 1-2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6-9% by weight of red ginseng powder, 13-16% by weight of Bokryeong powder, 43-47% by weight of raw rehmannia glutinosa powder, and 31-35% by weight of honey were mixed and mixed to prepare an herbal medicine. Obtaining a ring mixture (S9); It is characterized in that it comprises a step (S10) of manufacturing the oriental medicine ring mixture through a ventilation machine and then drying it to obtain the oriental medicine ring.

Description

Manufacturing method of oriental medicine pills containing lactic acid bacteria fermented kelp extract}

The present invention relates to a method for manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract. More specifically, it provides a lactic acid bacteria-fermented kelp extract that is beneficial to health by fermenting lactic acid bacteria using kelp, a seaweed abundant in the sea, and provides an herbal medicine that is easy to consume. It relates to a method of manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract, which is manufactured into an herbal pill in order to be effective.

As living standards have improved due to recent modernization, the intake of fat and sugar has increased and the intake of fiber has decreased. In particular, with the establishment of a dining out culture, the preference for high-protein diets is becoming more prominent. However, physical activity is decreasing and the number of obese people is rapidly increasing. As the consumption of high-calorie foods increases and fiber intake decreases, obesity, especially abdominal obesity, is emerging as a serious social problem.

Meanwhile, kelp, which belongs to the Kelp family of the brown algae plant Kelp order, is a perennial plant distributed along the coasts of the boreal and subarctic regions. There are approximately 20 or more species growing only on the Pacific coast, and it has been used as food in Korea, Japan, and China since ancient times. It's a sea horse.

The composition of the kelp varies depending on the type, but is generally about 16% moisture, 7% protein, 1.5% fat, 49% carbohydrate, and 26.5% inorganic salts. 20% of the carbohydrate is fiber, and the rest is physiological functional ingredients such as alginic acid and naminarin. It is a polysaccharide, and is also rich in minerals such as iodine, potassium, and calcium, various vitamins including vitamin B2, and amino acids, especially glutamic acid. In particular, indigestible polysaccharides, which are difficult to digest, are in the spotlight as dietary fibers as ingredients for various health foods, such as having an intestinal function, preventing obesity, anti-cancer, lowering cholesterol levels, and excreting heavy metals. Oligosaccharides made from these polysaccharides are generally indigestible, act as bifidus growth factors, and are known to be effective in preventing tooth decay. Recently, research has been actively conducted to produce oligosaccharides from alginic acid.

Because kelp contains many beneficial ingredients, various types of research using these active ingredients are being conducted.

Meanwhile, research on GABA has been actively underway since it was first discovered in mammalian brain extract by Florey and Robert in 1950. GABA has a molecular weight of 103.2 daltons and is called piperic acid. It has a melting point of 202°C, which is stable to heat. It has a molecular formula of C4H9NO2 and is highly soluble in water.

In general, GABA (Gamma Amino Butyric Acid) is a type of non-protein amino acid widely distributed in nature, exists in the brain and spinal cord of mammals, and is an inhibitory neurotransmitter. GABA is known to be involved in the regulation of many physiological mechanisms in the human body, activating blood flow to the brain and increasing oxygen supply to enhance the metabolic function of brain cells.

Recently, it has been announced that GABA not only significantly enhances learning ability and contributes to the promotion of long-term memory, but also suppresses the rise in blood pressure and acts as a factor in controlling appetite and satiety, making it one of the substances that is attracting attention around the world as an oral prescription drug. There are 5 and 10 mg pills on the market, but synthetic GABA preparations are said to have side effects on the digestive system, such as loss of appetite, constipation, and diarrhea.

Due to this role of GABA, interest in GABA not only as a medicine but also as a functional food ingredient has recently been increasing. GABA is found in large quantities in grains such as sprouted brown rice, sprouted grains, green tea, and cabbage roots, so it can be taken orally. However, the amount of GABA contained in them is not large, so it is not easy to consume the necessary amount from food to exert pharmacological action.

GABA, which has recently been proven to have excellent physiological effects in lowering blood pressure and promoting brain cell metabolism through clinical trials around the world, has been found to be produced through the precursor glutamic acid through the decarboxylation effect of GAD. It has been found to be produced worldwide, including in Japan. Although technology to increase the content of GABA is being developed, it mainly results in a fermentation technology that artificially increases the amount of chemical MSG and then converts it to GABA through fermentation, going against the current trend of requiring stability and naturalness. there is. To overcome this problem, research is actively being conducted to discover food materials containing large amounts of GABA and to utilize these plant materials industrially with high yields.

Registered Patent No. 10-1848373

Accordingly, the present invention was created to eliminate the above-mentioned problems, and provides a lactic acid bacteria-fermented kelp extract beneficial to health by fermenting lactic acid bacteria using kelp, which is an abundant seaweed in the sea. In addition, it is easy to consume and can be used as an herbal medicine pill to have an herbal medicine effect. It was completed as a technical task with a focus on the manufacturing method of oriental medicine pills containing lactic acid bacteria-fermented kelp extract.

In order to achieve the above technical problem, the present invention constitutes a method for manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract, in which the prepared kelp is washed, desalted, and then put into a grinder through a grinding process and crushed to obtain ground kelp. Step (S1); Putting the pulverized kelp into a heating container, adding purified water 15 to 17 times the volume of the kelp, and performing hot water extraction while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract (S2); Adding Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, to the hot water extract and stirring for 4 to 4.5 hours at pH 4.5, 60 to 63°C to obtain an enzymatic kelp extract (S3); After growing the lactic acid bacteria inoculated on a Lactobacilli MRS agar plate by culturing at 37-39°C for 2 days, the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and cultured at 37-39°C for 18-22 hours. Creating a pre-culture solution and then cultivating the pre-culture solution and the enzymatic kelp extract with shaking to obtain a kelp lactic acid bacteria fermentation product (S4); Filtering the cultured kelp lactic acid bacteria fermented product through a mesh sieve of 70 to 80 mesh, and then sterilizing the filtered kelp lactic acid bacteria fermented product at 120 to 125°C for 15 to 30 minutes under high temperature and pressure (S5); Concentrating the high-temperature autoclaved fermented kelp lactic acid bacteria under reduced pressure to 19 to 20 brix to obtain a concentrate, then transferring the concentrate to a tray and freezing it at -20 to -22°C for 24 to 26 hours (S6); Inserting the frozen fermented kelp lactic acid bacteria into a freeze dryer and drying it for 5 to 6 days (S7); Step (S8) of pulverizing the dried fermented kelp lactic acid bacteria in a grinder and then passing it through a mesh sieve of 58 to 60 mesh to obtain fermented kelp lactic acid bacteria powder (S8); 1-2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6-9% by weight of red ginseng powder, 13-16% by weight of Bokryeong powder, 43-47% by weight of raw rehmannia glutinosa powder, and 31-35% by weight of honey were mixed and mixed to prepare an herbal medicine. Obtaining a ring mixture (S9); It provides a method for producing an herbal ring containing lactic acid bacteria fermented kelp extract, comprising the step of manufacturing the herbal ring mixture through a ventilation machine and then drying it to obtain the herbal ring (S10). .

At this time, in the shaking culture (S4) step, 5 to 7 parts by weight of the preculture solution was inoculated with respect to 100 parts by weight of the enzyme kelp extract, and then cultured with shaking at 37 to 39 ° C. for 48 to 52 hours at 150 to 170 rpm. It is characterized by composition.

In addition, in the shaking culture (S4) step, the Lactobacilli MRS contains 0.23 to 0.25% by weight of ammonium citrate, 0.58 to 0.60% by weight of sodium acetate, 1.16 to 1.17% by weight of protease peptone, 1.16 to 1.17% by weight of beef extract, and 0.58% by weight of yeast extract. ∼0.60% by weight, dextrose 2.34∼2.36% by weight, magnesium sulfate 0.0058∼0.0060% by weight, polysorbate 93.67∼93.69% by weight, magnesium sulfate 0.010∼0.015% by weight, dipotassium phosphate 0.230∼0.235% by weight It consists of %, and the above ingredients It is characterized in that it further contains 1.5 to 2.5 parts by weight of Agar based on 100 parts by weight of Lactobacilli MRS.

According to the present invention, kelp, which is an abundant seaweed in the sea, is fermented with lactic acid bacteria to provide a lactic acid bacteria-fermented kelp extract that is beneficial to health. In addition, it is easy to consume and can be manufactured into oriental medicine pills to have oriental medicine effects. there is.

Figure 1 is an illustration of the process sequence of the method for manufacturing an herbal medicine pill containing lactic acid bacteria fermented kelp extract according to the present invention.

Hereinafter, specific details for implementing the present invention will be described in more detail with reference to the attached drawings.

The present invention provides a lactic acid bacteria-fermented kelp extract that is beneficial to health by fermenting lactic acid bacteria using kelp, which is an abundant seaweed in the sea, as well as providing an herbal pill containing fermented lactic acid kelp extract, which is manufactured into an herbal pill for easy ingestion and oriental medicine effects. Referring to FIG. 1, the manufacturing method includes steps S1 to S10, which will be described later.

The present invention is a method of manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract. To implement this, the prepared kelp is first washed, desalted, and then put through a grinding process and placed in a grinder to obtain ground kelp. (Step S1)

Then, the pulverized kelp is placed in a heating container, purified water 15 to 17 times the volume of the kelp is added, and hot water extraction is performed while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract. (S2 step)

The pretreatment process of the kelp prepared through steps S1 and S2 is completed.

Then, referring to [Table 1] below, Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, were added to the hot water extract and stirred at pH 4.5, 60-63°C for 4-4.5 hours to obtain an enzymatic kelp extract. (Step S3)

[Table 1]

The above process completes the enzyme treatment of the kelp hot water extract.

Meanwhile, the lactic acid bacteria inoculated on the Lactobacilli MRS agar plate were grown by culturing at 37-39°C for 2 days, and then the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and grown at 37-39°C for 18-22 hours. After culturing to create a pre-culture solution, the pre-culture solution and the enzymatic kelp extract are shaken and cultured to obtain kelp lactic acid bacteria fermentation product (step S4).

At this time, 5 to 7 parts by weight of the preculture solution is inoculated with respect to 100 parts by weight of the enzyme kelp extract, and then cultured with shaking at 37 to 39°C for 48 to 52 hours at 150 to 170 rpm.

In addition, the Lactobacilli MRS contains ammonium citrate 0.23-0.25% by weight, sodium acetate 0.58-0.60% by weight, protease peptone 1.16-1.17% by weight, beef extract 1.16-1.17% by weight, yeast extract 0.58-0.60% by weight, and dextrose 2.34-2. .36 % by weight, magnesium sulfate 0.0058-0.0060 wt%, polysorbate 93.67-93.69 wt%, magnesium sulfate 0.010-0.015 wt%, dipotassium phosphate 0.230-0.235 wt%, and Lactobacilli MRS 100 containing the above ingredients foster husband As a standard, it should be composed of an additional 1.5 to 2.5 parts by weight of Agar.

Through the above process, the lactic acid bacteria fermentation process of the kelp hot water extract is completed.

Then, the cultured kelp lactic acid bacteria fermented product is filtered through a mesh sieve of 70 to 80 mesh, and then the filtered kelp lactic acid bacteria fermented product is sterilized under high temperature and pressure at 120 to 125°C for 15 to 30 minutes. (Step S5)

Afterwards, the high-temperature autoclaved fermented kelp lactic acid bacteria is concentrated under reduced pressure to 19 to 20 brix to obtain a concentrate, and then the concentrate is transferred to a tray and frozen at -20 to -22°C for 24 to 26 hours. (Step S6)

Then, the frozen kelp lactic acid bacteria fermentation product is placed in a freeze dryer and dried for 5 to 6 days (step S7).

In the next process, the dried kelp lactic acid bacteria fermentation product is put into a grinder and pulverized, and then passed through a mesh sieve of 58 to 60 mesh to obtain kelp lactic acid bacteria fermentation powder (step S8).

Then, 1 to 2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6 to 9% by weight of red ginseng powder, 13 to 16% by weight of Bokryeong powder, 43 to 47% by weight of raw rehmannia glutinosa powder, and 31 to 35% by weight of honey were mixed and mixed. Obtain the herbal pill mixture. (Step S9)

Then, the herbal pill mixture is manufactured into an herbal pill through a ventilation machine, and then dried to obtain an herbal pill (step S10).

Through the above-described steps S1 to S10, the production of an herbal pill containing the lactic acid bacteria-fermented kelp extract to be implemented in the present invention is completed. According to the above-described invention, kelp, an abundant seaweed in the sea, is used to ferment lactic acid bacteria, which is beneficial to health. In addition to providing lactic acid bacteria-fermented kelp extract, it can be manufactured into herbal medicine pills to be easy to consume and have herbal medicine effects.

The present invention described above has been described with reference to an embodiment shown in the drawings, but this is merely illustrative, and various modifications and other equivalent embodiments can be made by those skilled in the art. should be clarified. Therefore, the true technical protection scope of the present invention should be interpreted in accordance with the attached claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

Claims (3)

In constructing a method for manufacturing an herbal medicine pill containing lactic acid bacteria fermented kelp extract,
Step (S1) of washing the prepared kelp, desalting it, going through a grinding process, putting it in a grinder, and pulverizing it to obtain ground kelp;
Putting the pulverized kelp into a heating container, adding purified water 15 to 17 times the volume of the kelp, and performing hot water extraction while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract (S2);
Adding Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, to the hot water extract and stirring for 4 to 4.5 hours at pH 4.5, 60 to 63°C to obtain an enzymatic kelp extract (S3);
After growing the lactic acid bacteria inoculated on a Lactobacilli MRS agar plate by culturing at 37-39°C for 2 days, the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and cultured at 37-39°C for 18-22 hours. Creating a pre-culture solution and then cultivating the pre-culture solution and the enzymatic kelp extract with shaking to obtain a kelp lactic acid bacteria fermentation product (S4);
Filtering the cultured kelp lactic acid bacteria fermented product through a mesh sieve of 70 to 80 mesh, and then sterilizing the filtered kelp lactic acid bacteria fermented product at 120 to 125°C for 15 to 30 minutes under high temperature and pressure (S5);
Concentrating the high-temperature autoclaved fermented kelp lactic acid bacteria under reduced pressure to 19 to 20 brix to obtain a concentrate, then transferring the concentrate to a tray and freezing it at -20 to -22°C for 24 to 26 hours (S6);
Inserting the frozen fermented kelp lactic acid bacteria into a freeze dryer and drying it for 5 to 6 days (S7);
Step (S8) of pulverizing the dried fermented kelp lactic acid bacteria in a grinder and then passing it through a mesh sieve of 58 to 60 mesh to obtain fermented kelp lactic acid bacteria powder (S8);
1-2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6-9% by weight of red ginseng powder, 13-16% by weight of Bokryeong powder, 43-47% by weight of raw rehmannia glutinosa powder, and 31-35% by weight of honey were mixed and mixed to prepare an herbal medicine. Obtaining a ring mixture (S9);
A method for producing an herbal ring containing lactic acid bacteria-fermented kelp extract, comprising: manufacturing the herbal ring mixture through a ventilation machine and then drying it to obtain the herbal ring (S10).
According to paragraph 1,
In the shaking culture (S4) step, 5 to 7 parts by weight of the pre-culture solution is inoculated with respect to 100 parts by weight of the enzyme kelp extract, and then cultured with shaking at 37 to 39°C for 48 to 52 hours at 150 to 170 rpm. A method of manufacturing an herbal pill containing lactic acid bacteria fermented kelp extract, characterized by:
According to paragraph 2,
In the shaking culture (S4) step, the Lactobacilli MRS contains 0.23 to 0.25% by weight of ammonium citrate, 0.58 to 0.60% by weight of sodium acetate, 1.16 to 1.17% by weight of protease peptone, 1.16 to 1.17% by weight of beef extract, and 0.58 to 0.60% by weight of yeast extract. % by weight, dextrose 2.34 to 2.36 wt%, magnesium sulfate 0.0058 to 0.0060 wt%, polysorbate 93.67 to 93.69 wt%, magnesium sulfate 0.010 to 0.015 wt%, and dipotassium phosphate 0.230 to 0.235 wt%. Lose,
A method for producing an herbal pill containing lactic acid bacteria-fermented kelp extract, characterized in that it further comprises 1.5 to 2.5 parts by weight of Agar based on 100 parts by weight of Lactobacilli MRS containing the above ingredients.

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