KR20240063265A - Manufacturing method of oriental medicine pills containing lactic acid bacteria fermented kelp extract - Google Patents

Manufacturing method of oriental medicine pills containing lactic acid bacteria fermented kelp extract Download PDF

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KR20240063265A
KR20240063265A KR1020220142333A KR20220142333A KR20240063265A KR 20240063265 A KR20240063265 A KR 20240063265A KR 1020220142333 A KR1020220142333 A KR 1020220142333A KR 20220142333 A KR20220142333 A KR 20220142333A KR 20240063265 A KR20240063265 A KR 20240063265A
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kelp
lactic acid
acid bacteria
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엄수현
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    • A23L3/10Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by heating materials in packages which are not progressively transported through the apparatus
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    • A23L3/00Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
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    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23P10/00Shaping or working of foodstuffs characterised by the products
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Abstract

본 발명은 유산균 발효 다시마추출물이 함유된 한방환의 제조방법에 관한 것으로 바다에 풍부한 해조류인 다시마를 이용하여 유산균 발효시켜 건강에 유익한 유산균 발효 다시마추출물을 제공함과 아울러, 섭취 용이하면서 한방효과를 가지기 위해 한방환으로 제조토록 하기 위해, 준비된 다시마를 수세하고, 탈염한 후, 분쇄공정을 거쳐 분쇄기에 투입 분쇄하여 분쇄다시마를 획득하는 단계(S1); 상기 분쇄다시마를 가열용기에 넣은 다음, 다시마 부피의 15∼17배 되는 정제수를 첨가한 후, 93∼98℃에서 4∼5.5시간 가열하면서 열수 추출하여 열수추출물을 획득하는 단계(S2); 상기 열수추출물에 분해효소인 Viscozyme L과 당화효소인 Saczyme을 투입 첨가한 후 pH 4.5, 60∼63℃에서 4∼4.5시간 교반하여 효소다시마추출물을 획득하는 단계(S3); Lactobacilli MRS agar plate에 접종된 유산균을 37∼39℃에서 2일간 배양하여 성장시킨 후, 상기 성장된 유산균 Bifidobacterium longum의 loop는 전 배양을 위한 배지에 접종하여 37∼39℃에서 18∼22시간 배양하여 전배양액을 만든 다음, 상기 전배양액과 효소다시마추출물을 진탕배양하여 다시마유산균발효물을 획득하는 단계(S4); 상기 배양 종료된 다시마유산균발효물을 70∼80mesh로 된 망체로 여과한 다음, 상기 여과된 다시마유산균발효물을 120∼125℃에서 15∼30분 동안 고온가압 멸균하는 단계(S5); 상기 고온가압 멸균된 다시마유산균발효물을 19∼20brix로 감압농축하여 농축액을 얻은 다음, 상기 농축액을 트레이에 옮긴 후 -20∼-22℃ 상에서 24∼26시간 동결하는 단계(S6); 상기 동결 완료된 다시마유산균발효물을 동결건조기에 투입하여 5∼6일간 건조하는 단계(S7); 상기 건조된 다시마유산균발효물을 분쇄기에 투입 분쇄한 후, 58∼60mesh로 된 망체에 통과시켜 다시마유산균발효물분말을 획득하는 단계(S8); 상기 획득된 다시마유산균발효물분말 1∼2중량%, 홍삼분말 6∼9중량%, 복령분말 13∼16중량%, 생지황분말 43∼47중량%, 꿀 31∼35중량%를 배합하여 혼합하여 한방환혼합물을 획득하는 단계(S9); 상기 한방환혼합물을 제환기를 통해 한방환을 제조한 후, 건조시켜 한방환을 획득하는 단계(S10);을 포함하여 이루어짐을 특징으로 한다.The present invention relates to a method of manufacturing an herbal medicine pill containing lactic acid bacteria-fermented kelp extract. It provides a lactic acid bacteria-fermented kelp extract that is beneficial to health by fermenting lactic acid bacteria using kelp, a seaweed abundant in the sea, and provides an herbal medicine pill that is easy to consume and has an herbal medicine effect. In order to manufacture it into rings, the prepared kelp is washed, desalted, and then put into a crusher through a crushing process to obtain crushed kelp (S1); Putting the pulverized kelp into a heating container, adding purified water 15 to 17 times the volume of the kelp, and performing hot water extraction while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract (S2); Adding Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, to the hot water extract and stirring for 4 to 4.5 hours at pH 4.5, 60 to 63°C to obtain an enzymatic kelp extract (S3); After growing the lactic acid bacteria inoculated on a Lactobacilli MRS agar plate by culturing at 37-39°C for 2 days, the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and cultured at 37-39°C for 18-22 hours. Creating a pre-culture solution and then cultivating the pre-culture solution and the enzymatic kelp extract with shaking to obtain a kelp lactic acid bacteria fermentation product (S4); Filtering the cultured kelp lactic acid bacteria fermented product through a mesh sieve of 70 to 80 mesh, and then sterilizing the filtered kelp lactic acid bacteria fermented product at 120 to 125°C for 15 to 30 minutes under high temperature and pressure (S5); Concentrating the high-temperature autoclaved fermented kelp lactic acid bacteria under reduced pressure to 19 to 20 brix to obtain a concentrate, then transferring the concentrate to a tray and freezing it at -20 to -22°C for 24 to 26 hours (S6); Inserting the frozen fermented kelp lactic acid bacteria into a freeze dryer and drying it for 5 to 6 days (S7); Step (S8) of pulverizing the dried fermented kelp lactic acid bacteria in a grinder and then passing it through a mesh sieve of 58 to 60 mesh to obtain fermented kelp lactic acid bacteria powder (S8); 1-2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6-9% by weight of red ginseng powder, 13-16% by weight of Bokryeong powder, 43-47% by weight of raw rehmannia glutinosa powder, and 31-35% by weight of honey were mixed and mixed to prepare an herbal medicine. Obtaining a ring mixture (S9); It is characterized in that it comprises a step (S10) of manufacturing the oriental medicine ring mixture through a ventilation machine and then drying it to obtain the oriental medicine ring.

Description

유산균 발효 다시마추출물이 함유된 한방환의 제조방법{Manufacturing method of oriental medicine pills containing lactic acid bacteria fermented kelp extract}Manufacturing method of oriental medicine pills containing lactic acid bacteria fermented kelp extract}

본 발명은 유산균 발효 다시마추출물이 함유된 한방환의 제조방법에 관한 것으로서, 보다 상세하게는 바다에 풍부한 해조류인 다시마를 이용하여 유산균 발효시켜 건강에 유익한 유산균 발효 다시마추출물을 제공함과 아울러, 섭취 용이하면서 한방효과를 가지기 위해 한방환으로 제조토록 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조방법에 관한 것이다.The present invention relates to a method for manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract. More specifically, it provides a lactic acid bacteria-fermented kelp extract that is beneficial to health by fermenting lactic acid bacteria using kelp, a seaweed abundant in the sea, and provides an herbal medicine that is easy to consume. It relates to a method of manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract, which is manufactured into an herbal pill in order to be effective.

최근 현대화에 따라 생활 수준이 향상됨으로써 지방과 당분의 섭취는 증가하고 섬유질의 섭취는 감소하였다. 특히, 외식문화의 정착으로 고단백 위주의 식단 선호 현상은 두드러지고 있다. 하지만, 육체적인 활동은 감소하여 비만 인구가 급속히 늘고 있는 실정이다. 고열량 식품의 섭취가 증가하고 섬유질 섭취가 줄어들면서 비만, 특히 복부 비만이 사회적으로 심각한 문제로 떠오르고 있는 것이다.As living standards have improved due to recent modernization, the intake of fat and sugar has increased and the intake of fiber has decreased. In particular, with the establishment of a dining out culture, the preference for high-protein diets is becoming more prominent. However, physical activity is decreasing and the number of obese people is rapidly increasing. As the consumption of high-calorie foods increases and fiber intake decreases, obesity, especially abdominal obesity, is emerging as a serious social problem.

한편, 갈조식물 다시마목 다시마과에 속하는 다시마는 한대, 아한대의 연안에 분포하는 한해성 식물로서, 태평양 연안에만 대략 20여 종 이상이 자생하고 있으며, 예로부터는 우리나라와 일본, 중국 등에서 식용으로 사용해 온 바닷말이다.Meanwhile, kelp, which belongs to the Kelp family of the brown algae plant Kelp order, is a perennial plant distributed along the coasts of the boreal and subarctic regions. There are approximately 20 or more species growing only on the Pacific coast, and it has been used as food in Korea, Japan, and China since ancient times. It's a sea horse.

상기 다시마의 성분은 종류에 따라서 다르지만, 대체로 수분 16%, 단백질 7%, 지방 1.5%, 탄수화물 49%, 무기염류 26.5% 정도이며, 탄수화물의 20%는 섬유소이고 나머지는 알긴산과 나미나린 등 생리기능성이 있는 다당류이며, 그 외에, 요오드, 칼륨, 칼슘 등의 무기질, 비타민 B2를 비롯한 각종 비타민, 아미노산, 특히 글루탐산의 함량이 풍부하다. 특히 소화가 어려운 난소화성의 다당류는 식이섬유로서 정장작용과 비만방지, 항암, 콜레스테롤치 저하, 중금속의 배출 등 각종 건강식품의 소재로서 각광을 받고 있다. 이러한 다당류로부터 만들어지는 올리고당은 일반적으로 난소화성이고, 비피더스 증식인자로 작용하며, 충치예방에도 효과가 있는 것으로 알려지고 있어, 최근에는 알긴산으로부터 올리고당을 제조하려는 연구가 활발히 이루어지고 있다.The composition of the kelp varies depending on the type, but is generally about 16% moisture, 7% protein, 1.5% fat, 49% carbohydrate, and 26.5% inorganic salts. 20% of the carbohydrate is fiber, and the rest is physiological functional ingredients such as alginic acid and naminarin. It is a polysaccharide, and is also rich in minerals such as iodine, potassium, and calcium, various vitamins including vitamin B2, and amino acids, especially glutamic acid. In particular, indigestible polysaccharides, which are difficult to digest, are in the spotlight as dietary fibers as ingredients for various health foods, such as having an intestinal function, preventing obesity, anti-cancer, lowering cholesterol levels, and excreting heavy metals. Oligosaccharides made from these polysaccharides are generally indigestible, act as bifidus growth factors, and are known to be effective in preventing tooth decay. Recently, research has been actively conducted to produce oligosaccharides from alginic acid.

이와 같이 다시마에는 각종 유익한 성분이 많이 함유되어 있기 때문에, 이러한 유효성분을 이용하는 다양한 형태의 연구가 진행되고 있다.Because kelp contains many beneficial ingredients, various types of research using these active ingredients are being conducted.

한편, GABA는 1950년 Florey와 Robert에 의해 포유류의 뇌 추출액에서 처음 발견된 이래 연구가 활발하게 진행되고 있다. GABA는 분자량이 103.2 달톤 (dalton)으로 피페리인산으로 불리며 용융점이 202℃로 열에 안정한 편이며 C4H9NO2의 분자식을 가지며 물에 대한 용해성이 크다.Meanwhile, research on GABA has been actively underway since it was first discovered in mammalian brain extract by Florey and Robert in 1950. GABA has a molecular weight of 103.2 daltons and is called piperic acid. It has a melting point of 202°C, which is stable to heat. It has a molecular formula of C4H9NO2 and is highly soluble in water.

일반적으로 GABA (Gamma Amino Butyric Acid)는 자연계에 널리 분포하는 비단백질 아미노산의 일종으로 포유동물의 뇌나 척수에 존재하며 흥분 억제성 신경전달물질 (Inhibitory Neurotransmitter)이다. GABA는 인체의 많은 생리적인 메커니즘의 조절에 관여하여 뇌의 혈류를 활발하게 하고 산소 공급량을 증가시켜 뇌세포의 대사기능을 항진시키는 작용을 하는 것으로 알려져 있다.In general, GABA (Gamma Amino Butyric Acid) is a type of non-protein amino acid widely distributed in nature, exists in the brain and spinal cord of mammals, and is an inhibitory neurotransmitter. GABA is known to be involved in the regulation of many physiological mechanisms in the human body, activating blood flow to the brain and increasing oxygen supply to enhance the metabolic function of brain cells.

최근에는 GABA가 학습능력을 유의적으로 증강시키고 장기 기억 촉진에 기여할 뿐만 아니라 혈압상승을 억제하며 식용과 포만감을 조절하는 요소로 작용한다고 발표되어 전 세계적으로 주목을 받고 있는 물질의 하나로서 경구적 처방약으로서는 5, 10mg의 환제가 시판되고 있으나 의약품의 합성 GABA 제제의 경우 식욕부진, 변비, 설사 등의 소화기계의 부작용이 있다고 한다.Recently, it has been announced that GABA not only significantly enhances learning ability and contributes to the promotion of long-term memory, but also suppresses the rise in blood pressure and acts as a factor in controlling appetite and satiety, making it one of the substances that is attracting attention around the world as an oral prescription drug. There are 5 and 10 mg pills on the market, but synthetic GABA preparations are said to have side effects on the digestive system, such as loss of appetite, constipation, and diarrhea.

이러한 GABA의 역할로 인해 의약품으로서 뿐만 아니라, 최근에는 기능성 식품소재로서의 GABA에 대한 관심이 고조되고 있다. GABA는 발아현미를 비롯한 발아곡류, 녹차, 배추 뿌리 등 곡물에서도 많이 검출되고 있으므로 경구적으로 섭취하는 것이 가능하다. 그러나 이들에 함유된 GABA의 양은 많지 않아 약리작용을 발휘하기 위한 필요량을 식품에서 섭취하는 것은 용이하지 않다.Due to this role of GABA, interest in GABA not only as a medicine but also as a functional food ingredient has recently been increasing. GABA is found in large quantities in grains such as sprouted brown rice, sprouted grains, green tea, and cabbage roots, so it can be taken orally. However, the amount of GABA contained in them is not large, so it is not easy to consume the necessary amount from food to exert pharmacological action.

최근 전 세계적으로 임상시험을 통한 혈압저하, 뇌세포 대사촉진 등에 탁월한 생리활성 효과가 있는 것으로 검증된 GABA의 경우 GAD의 탈탄산작용에 의하여 전구물질인 글루탐산을 통하여 생성됨이 규명되었는데 일본을 비롯하여 전세계적으로 GABA의 함유량을 증대시키기 위한 기술이 개발 중이지만 주로 화학적 MSG를 인위적으로 첨가 증량한 이후 이의 발효를 통하여 GABA로 전환시키는 발효기술로 귀결되고 있는 실정이어서 현재 안정성 및 천연성을 요구하는 흐름을 거스르고 있다. 이를 극복하기 위해 GABA가 다량 함유된 식품소재를 발굴하여 이러한 식물소재로부터 높은 수율의 산업적으로 활용하려는 연구가 활발히 진행되고 있는 실정이다.GABA, which has recently been proven to have excellent physiological effects in lowering blood pressure and promoting brain cell metabolism through clinical trials around the world, has been found to be produced through the precursor glutamic acid through the decarboxylation effect of GAD. It has been found to be produced worldwide, including in Japan. Although technology to increase the content of GABA is being developed, it mainly results in a fermentation technology that artificially increases the amount of chemical MSG and then converts it to GABA through fermentation, going against the current trend of requiring stability and naturalness. there is. To overcome this problem, research is actively being conducted to discover food materials containing large amounts of GABA and to utilize these plant materials industrially with high yields.

등록특허 제10-1848373호Registered Patent No. 10-1848373

이에 본 발명은 상기한 문제점을 일소하기 위해 창안한 것으로서, 바다에 풍부한 해조류인 다시마를 이용하여 유산균 발효시켜 건강에 유익한 유산균 발효 다시마추출물을 제공함과 아울러, 섭취 용이하면서 한방효과를 가지기 위해 한방환으로 제조토록 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조방법에 주안점을 두고 그 기술적 과제로서 완성한 것이다.Accordingly, the present invention was created to eliminate the above-mentioned problems, and provides a lactic acid bacteria-fermented kelp extract beneficial to health by fermenting lactic acid bacteria using kelp, which is an abundant seaweed in the sea. In addition, it is easy to consume and can be used as an herbal medicine pill to have an herbal medicine effect. It was completed as a technical task with a focus on the manufacturing method of oriental medicine pills containing lactic acid bacteria-fermented kelp extract.

위 기술적 과제를 달성하기 위한 본 발명은, 유산균 발효 다시마추출물이 함유된 한방환의 제조방법을 구성함에 있어서, 준비된 다시마를 수세하고, 탈염한 후, 분쇄공정을 거쳐 분쇄기에 투입 분쇄하여 분쇄다시마를 획득하는 단계(S1); 상기 분쇄다시마를 가열용기에 넣은 다음, 다시마 부피의 15∼17배 되는 정제수를 첨가한 후, 93∼98℃에서 4∼5.5시간 가열하면서 열수 추출하여 열수추출물을 획득하는 단계(S2); 상기 열수추출물에 분해효소인 Viscozyme L과 당화효소인 Saczyme을 투입 첨가한 후 pH 4.5, 60∼63℃에서 4∼4.5시간 교반하여 효소다시마추출물을 획득하는 단계(S3); Lactobacilli MRS agar plate에 접종된 유산균을 37∼39℃에서 2일간 배양하여 성장시킨 후, 상기 성장된 유산균 Bifidobacterium longum의 loop는 전 배양을 위한 배지에 접종하여 37∼39℃에서 18∼22시간 배양하여 전배양액을 만든 다음, 상기 전배양액과 효소다시마추출물을 진탕배양하여 다시마유산균발효물을 획득하는 단계(S4); 상기 배양 종료된 다시마유산균발효물을 70∼80mesh로 된 망체로 여과한 다음, 상기 여과된 다시마유산균발효물을 120∼125℃에서 15∼30분 동안 고온가압 멸균하는 단계(S5); 상기 고온가압 멸균된 다시마유산균발효물을 19∼20brix로 감압농축하여 농축액을 얻은 다음, 상기 농축액을 트레이에 옮긴 후 -20∼-22℃ 상에서 24∼26시간 동결하는 단계(S6); 상기 동결 완료된 다시마유산균발효물을 동결건조기에 투입하여 5∼6일간 건조하는 단계(S7); 상기 건조된 다시마유산균발효물을 분쇄기에 투입 분쇄한 후, 58∼60mesh로 된 망체에 통과시켜 다시마유산균발효물분말을 획득하는 단계(S8); 상기 획득된 다시마유산균발효물분말 1∼2중량%, 홍삼분말 6∼9중량%, 복령분말 13∼16중량%, 생지황분말 43∼47중량%, 꿀 31∼35중량%를 배합하여 혼합하여 한방환혼합물을 획득하는 단계(S9); 상기 한방환혼합물을 제환기를 통해 한방환을 제조한 후, 건조시켜 한방환을 획득하는 단계(S10);을 포함하여 이루어짐을 특징으로 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조방법을 제공한다.In order to achieve the above technical problem, the present invention constitutes a method for manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract, in which the prepared kelp is washed, desalted, and then put into a grinder through a grinding process and crushed to obtain ground kelp. Step (S1); Putting the pulverized kelp into a heating container, adding purified water 15 to 17 times the volume of the kelp, and performing hot water extraction while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract (S2); Adding Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, to the hot water extract and stirring for 4 to 4.5 hours at pH 4.5, 60 to 63°C to obtain an enzymatic kelp extract (S3); After growing the lactic acid bacteria inoculated on a Lactobacilli MRS agar plate by culturing at 37-39°C for 2 days, the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and cultured at 37-39°C for 18-22 hours. Creating a pre-culture solution and then cultivating the pre-culture solution and the enzymatic kelp extract with shaking to obtain a kelp lactic acid bacteria fermentation product (S4); Filtering the cultured kelp lactic acid bacteria fermented product through a mesh sieve of 70 to 80 mesh, and then sterilizing the filtered kelp lactic acid bacteria fermented product at 120 to 125°C for 15 to 30 minutes under high temperature and pressure (S5); Concentrating the high-temperature autoclaved fermented kelp lactic acid bacteria under reduced pressure to 19 to 20 brix to obtain a concentrate, then transferring the concentrate to a tray and freezing it at -20 to -22°C for 24 to 26 hours (S6); Inserting the frozen fermented kelp lactic acid bacteria into a freeze dryer and drying it for 5 to 6 days (S7); Step (S8) of pulverizing the dried fermented kelp lactic acid bacteria in a grinder and then passing it through a mesh sieve of 58 to 60 mesh to obtain fermented kelp lactic acid bacteria powder (S8); 1-2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6-9% by weight of red ginseng powder, 13-16% by weight of Bokryeong powder, 43-47% by weight of raw rehmannia glutinosa powder, and 31-35% by weight of honey were mixed and mixed to prepare an herbal medicine. Obtaining a ring mixture (S9); It provides a method for producing an herbal ring containing lactic acid bacteria fermented kelp extract, comprising the step of manufacturing the herbal ring mixture through a ventilation machine and then drying it to obtain the herbal ring (S10). .

이때, 상기 진탕배양(S4) 단계에서, 상기 효소다시마추출물 100중량부에 대해 전배양액 5∼7중량부를 접종한 후, 37∼39℃에서 48∼52시간 동안 150∼170rpm의 조건하에서 진탕배양되도록 구성함을 특징으로 한다.At this time, in the shaking culture (S4) step, 5 to 7 parts by weight of the preculture solution was inoculated with respect to 100 parts by weight of the enzyme kelp extract, and then cultured with shaking at 37 to 39 ° C. for 48 to 52 hours at 150 to 170 rpm. It is characterized by composition.

또한, 상기 진탕배양(S4) 단계에서, 상기 Lactobacilli MRS는 ammonium citrate 0.23∼0.25중량%, sodium acetate 0.58∼0.60중량%, protease peptone 1.16∼1.17중량%, beef extract 1.16∼1.17중량%, yeast extract 0.58∼0.60중량%, dextrose 2.34∼2.36중량%, magnese sulfate 0.0058∼0.0060중량%, polysorbate 93.67∼93.69중량%, magnesium sulfate 0.010∼0.015중량%, dipotassium phosphate 0.230∼0.235중량%를 포함하여 이루어지며, 상기 성분들이 포함된 Lactobacilli MRS 100중량부를 기준으로 Agar 1.5∼2.5중량부를 더 포함하여 구성됨을 특징으로 한다.In addition, in the shaking culture (S4) step, the Lactobacilli MRS contains 0.23 to 0.25% by weight of ammonium citrate, 0.58 to 0.60% by weight of sodium acetate, 1.16 to 1.17% by weight of protease peptone, 1.16 to 1.17% by weight of beef extract, and 0.58% by weight of yeast extract. ∼0.60% by weight, dextrose 2.34∼2.36% by weight, magnesium sulfate 0.0058∼0.0060% by weight, polysorbate 93.67∼93.69% by weight, magnesium sulfate 0.010∼0.015% by weight, dipotassium phosphate 0.230∼0.235% by weight It consists of %, and the above ingredients It is characterized in that it further contains 1.5 to 2.5 parts by weight of Agar based on 100 parts by weight of Lactobacilli MRS.

상기한 본 발명에 의하면 바다에 풍부한 해조류인 다시마를 이용하여 유산균 발효시켜 건강에 유익한 유산균 발효 다시마추출물을 제공함과 아울러, 섭취 용이하면서 한방효과를 가지기 위해 한방환으로 제조토록 할 수 있는 등의 효과가 있다.According to the present invention, kelp, which is an abundant seaweed in the sea, is fermented with lactic acid bacteria to provide a lactic acid bacteria-fermented kelp extract that is beneficial to health. In addition, it is easy to consume and can be manufactured into oriental medicine pills to have oriental medicine effects. there is.

도 1은 본 발명에 의한 유산균 발효 다시마추출물이 함유된 한방환의 제조방법의 공정순서 예시도Figure 1 is an illustration of the process sequence of the method for manufacturing an herbal medicine pill containing lactic acid bacteria fermented kelp extract according to the present invention.

이하 본 발명의 실시를 위한 구체적인 내용을 첨부한 도면을 참조하여 더욱 상세하게 설명한다.Hereinafter, specific details for implementing the present invention will be described in more detail with reference to the attached drawings.

본 발명은 바다에 풍부한 해조류인 다시마를 이용하여 유산균 발효시켜 건강에 유익한 유산균 발효 다시마추출물을 제공함과 아울러, 섭취 용이하면서 한방효과를 가지기 위해 한방환으로 제조토록 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조방법에 관한 것으로서 도 1을 참조하여 보면 후술되는 S1∼S10단계를 포함하여 이루어진다.The present invention provides a lactic acid bacteria-fermented kelp extract that is beneficial to health by fermenting lactic acid bacteria using kelp, which is an abundant seaweed in the sea, as well as providing an herbal pill containing fermented lactic acid kelp extract, which is manufactured into an herbal pill for easy ingestion and oriental medicine effects. Referring to FIG. 1, the manufacturing method includes steps S1 to S10, which will be described later.

본 발명은 유산균 발효 다시마추출물이 함유된 한방환의 제조방법으로서 이의 구현을 위해 먼저 준비된 다시마를 수세하고, 탈염한 후, 분쇄공정을 거쳐 분쇄기에 투입 분쇄하여 분쇄다시마를 획득한다.(S1단계)The present invention is a method of manufacturing an herbal pill containing lactic acid bacteria-fermented kelp extract. To implement this, the prepared kelp is first washed, desalted, and then put through a grinding process and placed in a grinder to obtain ground kelp. (Step S1)

그리고 난 후, 상기 분쇄다시마를 가열용기에 넣은 다음, 다시마 부피의 15∼17배 되는 정제수를 첨가한 후, 93∼98℃에서 4∼5.5시간 가열하면서 열수 추출하여 열수추출물을 획득한다.(S2단계)Then, the pulverized kelp is placed in a heating container, purified water 15 to 17 times the volume of the kelp is added, and hot water extraction is performed while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract. (S2 step)

상기 S1 및 S2단계를 거쳐 준비된 다시마의 전처리공정이 완료된다.The pretreatment process of the kelp prepared through steps S1 and S2 is completed.

그런 다음, 아래 [표 1]을 참고하여, 상기 열수추출물에 분해효소인 Viscozyme L과 당화효소인 Saczyme을 투입 첨가한 후 pH 4.5, 60∼63℃에서 4∼4.5시간 교반하여 효소다시마추출물을 획득한다.(S3단계)Then, referring to [Table 1] below, Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, were added to the hot water extract and stirred at pH 4.5, 60-63°C for 4-4.5 hours to obtain an enzymatic kelp extract. (Step S3)

[표 1][Table 1]

Figure pat00001
Figure pat00001

위 공정으로 다시마 열수추출물의 효소처리가 완료된다.The above process completes the enzyme treatment of the kelp hot water extract.

한편, Lactobacilli MRS agar plate에 접종된 유산균을 37∼39℃에서 2일간 배양하여 성장시킨 후, 상기 성장된 유산균 Bifidobacterium longum의 loop는 전 배양을 위한 배지에 접종하여 37∼39℃에서 18∼22시간 배양하여 전배양액을 만든 다음, 상기 전배양액과 효소다시마추출물을 진탕배양하여 다시마유산균발효물을 획득한다.(S4단계)Meanwhile, the lactic acid bacteria inoculated on the Lactobacilli MRS agar plate were grown by culturing at 37-39°C for 2 days, and then the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and grown at 37-39°C for 18-22 hours. After culturing to create a pre-culture solution, the pre-culture solution and the enzymatic kelp extract are shaken and cultured to obtain kelp lactic acid bacteria fermentation product (step S4).

이때, 상기 효소다시마추출물 100중량부에 대해 전배양액 5∼7중량부를 접종한 후, 37∼39℃에서 48∼52시간 동안 150∼170rpm의 조건하에서 진탕배양되도록 구성한다.At this time, 5 to 7 parts by weight of the preculture solution is inoculated with respect to 100 parts by weight of the enzyme kelp extract, and then cultured with shaking at 37 to 39°C for 48 to 52 hours at 150 to 170 rpm.

그리고, 상기 Lactobacilli MRS는 ammonium citrate 0.23∼0.25중량%, sodium acetate 0.58∼0.60중량%, protease peptone 1.16∼1.17중량%, beef extract 1.16∼1.17중량%, yeast extract 0.58∼0.60중량%, dextrose 2.34∼2.36중량%, magnese sulfate 0.0058∼0.0060중량%, polysorbate 93.67∼93.69중량%, magnesium sulfate 0.010∼0.015중량%, dipotassium phosphate 0.230∼0.235중량%를 포함하여 이루어지도록 하며, 상기 성분들이 포함된 Lactobacilli MRS 100중량부를 기준으로 Agar 1.5∼2.5중량부를 더 포함하여 구성되도록 한다.In addition, the Lactobacilli MRS contains ammonium citrate 0.23-0.25% by weight, sodium acetate 0.58-0.60% by weight, protease peptone 1.16-1.17% by weight, beef extract 1.16-1.17% by weight, yeast extract 0.58-0.60% by weight, and dextrose 2.34-2. .36 % by weight, magnesium sulfate 0.0058-0.0060 wt%, polysorbate 93.67-93.69 wt%, magnesium sulfate 0.010-0.015 wt%, dipotassium phosphate 0.230-0.235 wt%, and Lactobacilli MRS 100 containing the above ingredients foster husband As a standard, it should be composed of an additional 1.5 to 2.5 parts by weight of Agar.

위 공정으로 다시마 열수추출물의 유산균발효공정이 완료된다.Through the above process, the lactic acid bacteria fermentation process of the kelp hot water extract is completed.

그런 다음, 상기 배양 종료된 다시마유산균발효물을 70∼80mesh로 된 망체로 여과한 다음, 상기 여과된 다시마유산균발효물을 120∼125℃에서 15∼30분 동안 고온가압 멸균한다.(S5단계)Then, the cultured kelp lactic acid bacteria fermented product is filtered through a mesh sieve of 70 to 80 mesh, and then the filtered kelp lactic acid bacteria fermented product is sterilized under high temperature and pressure at 120 to 125°C for 15 to 30 minutes. (Step S5)

그 후, 상기 고온가압 멸균된 다시마유산균발효물을 19∼20brix로 감압농축하여 농축액을 얻은 다음, 상기 농축액을 트레이에 옮긴 후 -20∼-22℃ 상에서 24∼26시간 동결한다.(S6단계)Afterwards, the high-temperature autoclaved fermented kelp lactic acid bacteria is concentrated under reduced pressure to 19 to 20 brix to obtain a concentrate, and then the concentrate is transferred to a tray and frozen at -20 to -22°C for 24 to 26 hours. (Step S6)

그리고 나서, 상기 동결 완료된 다시마유산균발효물을 동결건조기에 투입하여 5∼6일간 건조한다.(S7단계)Then, the frozen kelp lactic acid bacteria fermentation product is placed in a freeze dryer and dried for 5 to 6 days (step S7).

다음 공정으로서, 상기 건조된 다시마유산균발효물을 분쇄기에 투입 분쇄한 후, 58∼60mesh로 된 망체에 통과시켜 다시마유산균발효물분말을 획득한다.(S8단계)In the next process, the dried kelp lactic acid bacteria fermentation product is put into a grinder and pulverized, and then passed through a mesh sieve of 58 to 60 mesh to obtain kelp lactic acid bacteria fermentation powder (step S8).

그리고, 상기 획득된 다시마유산균발효물분말 1∼2중량%, 홍삼분말 6∼9중량%, 복령분말 13∼16중량%, 생지황분말 43∼47중량%, 꿀 31∼35중량%를 배합하여 혼합하여 한방환혼합물을 획득한다.(S9단계)Then, 1 to 2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6 to 9% by weight of red ginseng powder, 13 to 16% by weight of Bokryeong powder, 43 to 47% by weight of raw rehmannia glutinosa powder, and 31 to 35% by weight of honey were mixed and mixed. Obtain the herbal pill mixture. (Step S9)

그런 후, 상기 한방환혼합물을 제환기를 통해 한방환을 제조한 후, 건조시켜 한방환을 획득한다.(S10단계)Then, the herbal pill mixture is manufactured into an herbal pill through a ventilation machine, and then dried to obtain an herbal pill (step S10).

상기한 S1∼S10단계를 통해 본 발명에서 구현하고자 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조가 완료되며, 상술된 본 발명에 의하면, 바다에 풍부한 해조류인 다시마를 이용하여 유산균 발효시켜 건강에 유익한 유산균 발효 다시마추출물을 제공함과 아울러, 섭취 용이하면서 한방효과를 가지기 위해 한방환으로 제조할 수 있게 된다.Through the above-described steps S1 to S10, the production of an herbal pill containing the lactic acid bacteria-fermented kelp extract to be implemented in the present invention is completed. According to the above-described invention, kelp, an abundant seaweed in the sea, is used to ferment lactic acid bacteria, which is beneficial to health. In addition to providing lactic acid bacteria-fermented kelp extract, it can be manufactured into herbal medicine pills to be easy to consume and have herbal medicine effects.

이상에서 설명한 본 발명은, 도면에 도시된 일실시 예를 참고로 설명되었으나 이는 예시적인 것에 불과하며, 본 기술 분야의 통상의 지식을 가진 자라면 이로부터 다양한 변형 및 균등한 타 실시 예가 가능하다는 점을 명확히 하여야 할 것이다. 따라서, 본 발명의 진정한 기술적 보호 범위는 첨부된 청구범위에 의하여 해석되어야 하며, 그와 동등한 범위 내에 있는 모든 기술적 사상은 본 발명의 권리범위에 포함되는 것으로 해석되어야 할 것이다.The present invention described above has been described with reference to an embodiment shown in the drawings, but this is merely illustrative, and various modifications and other equivalent embodiments can be made by those skilled in the art. should be clarified. Therefore, the true technical protection scope of the present invention should be interpreted in accordance with the attached claims, and all technical ideas within the equivalent scope should be interpreted as being included in the scope of the present invention.

Claims (3)

유산균 발효 다시마추출물이 함유된 한방환의 제조방법을 구성함에 있어서,
준비된 다시마를 수세하고, 탈염한 후, 분쇄공정을 거쳐 분쇄기에 투입 분쇄하여 분쇄다시마를 획득하는 단계(S1);
상기 분쇄다시마를 가열용기에 넣은 다음, 다시마 부피의 15∼17배 되는 정제수를 첨가한 후, 93∼98℃에서 4∼5.5시간 가열하면서 열수 추출하여 열수추출물을 획득하는 단계(S2);
상기 열수추출물에 분해효소인 Viscozyme L과 당화효소인 Saczyme을 투입 첨가한 후 pH 4.5, 60∼63℃에서 4∼4.5시간 교반하여 효소다시마추출물을 획득하는 단계(S3);
Lactobacilli MRS agar plate에 접종된 유산균을 37∼39℃에서 2일간 배양하여 성장시킨 후, 상기 성장된 유산균 Bifidobacterium longum의 loop는 전 배양을 위한 배지에 접종하여 37∼39℃에서 18∼22시간 배양하여 전배양액을 만든 다음, 상기 전배양액과 효소다시마추출물을 진탕배양하여 다시마유산균발효물을 획득하는 단계(S4);
상기 배양 종료된 다시마유산균발효물을 70∼80mesh로 된 망체로 여과한 다음, 상기 여과된 다시마유산균발효물을 120∼125℃에서 15∼30분 동안 고온가압 멸균하는 단계(S5);
상기 고온가압 멸균된 다시마유산균발효물을 19∼20brix로 감압농축하여 농축액을 얻은 다음, 상기 농축액을 트레이에 옮긴 후 -20∼-22℃ 상에서 24∼26시간 동결하는 단계(S6);
상기 동결 완료된 다시마유산균발효물을 동결건조기에 투입하여 5∼6일간 건조하는 단계(S7);
상기 건조된 다시마유산균발효물을 분쇄기에 투입 분쇄한 후, 58∼60mesh로 된 망체에 통과시켜 다시마유산균발효물분말을 획득하는 단계(S8);
상기 획득된 다시마유산균발효물분말 1∼2중량%, 홍삼분말 6∼9중량%, 복령분말 13∼16중량%, 생지황분말 43∼47중량%, 꿀 31∼35중량%를 배합하여 혼합하여 한방환혼합물을 획득하는 단계(S9);
상기 한방환혼합물을 제환기를 통해 한방환을 제조한 후, 건조시켜 한방환을 획득하는 단계(S10);을 포함하여 이루어짐을 특징으로 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조방법.
In constructing a method for manufacturing an herbal medicine pill containing lactic acid bacteria fermented kelp extract,
Step (S1) of washing the prepared kelp, desalting it, going through a grinding process, putting it in a grinder, and pulverizing it to obtain ground kelp;
Putting the pulverized kelp into a heating container, adding purified water 15 to 17 times the volume of the kelp, and performing hot water extraction while heating at 93 to 98° C. for 4 to 5.5 hours to obtain a hot water extract (S2);
Adding Viscozyme L, a decomposition enzyme, and Saczyme, a saccharification enzyme, to the hot water extract and stirring for 4 to 4.5 hours at pH 4.5, 60 to 63°C to obtain an enzymatic kelp extract (S3);
After growing the lactic acid bacteria inoculated on a Lactobacilli MRS agar plate by culturing at 37-39°C for 2 days, the loop of the grown lactic acid bacteria Bifidobacterium longum was inoculated into the medium for pre-culture and cultured at 37-39°C for 18-22 hours. Creating a pre-culture solution and then cultivating the pre-culture solution and the enzymatic kelp extract with shaking to obtain a kelp lactic acid bacteria fermentation product (S4);
Filtering the cultured kelp lactic acid bacteria fermented product through a mesh sieve of 70 to 80 mesh, and then sterilizing the filtered kelp lactic acid bacteria fermented product at 120 to 125°C for 15 to 30 minutes under high temperature and pressure (S5);
Concentrating the high-temperature autoclaved fermented kelp lactic acid bacteria under reduced pressure to 19 to 20 brix to obtain a concentrate, then transferring the concentrate to a tray and freezing it at -20 to -22°C for 24 to 26 hours (S6);
Inserting the frozen fermented kelp lactic acid bacteria into a freeze dryer and drying it for 5 to 6 days (S7);
Step (S8) of pulverizing the dried fermented kelp lactic acid bacteria in a grinder and then passing it through a mesh sieve of 58 to 60 mesh to obtain fermented kelp lactic acid bacteria powder (S8);
1-2% by weight of the obtained kelp lactic acid bacteria fermentation powder, 6-9% by weight of red ginseng powder, 13-16% by weight of Bokryeong powder, 43-47% by weight of raw rehmannia glutinosa powder, and 31-35% by weight of honey were mixed and mixed to prepare an herbal medicine. Obtaining a ring mixture (S9);
A method for producing an herbal ring containing lactic acid bacteria-fermented kelp extract, comprising: manufacturing the herbal ring mixture through a ventilation machine and then drying it to obtain the herbal ring (S10).
제1항에 있어서,
상기 진탕배양(S4) 단계에서, 상기 효소다시마추출물 100중량부에 대해 전배양액 5∼7중량부를 접종한 후, 37∼39℃에서 48∼52시간 동안 150∼170rpm의 조건하에서 진탕배양되도록 구성함을 특징으로 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조방법.
According to paragraph 1,
In the shaking culture (S4) step, 5 to 7 parts by weight of the pre-culture solution is inoculated with respect to 100 parts by weight of the enzyme kelp extract, and then cultured with shaking at 37 to 39°C for 48 to 52 hours at 150 to 170 rpm. A method of manufacturing an herbal pill containing lactic acid bacteria fermented kelp extract, characterized by:
제2항에 있어서,
상기 진탕배양(S4) 단계에서, 상기 Lactobacilli MRS는 ammonium citrate 0.23∼0.25중량%, sodium acetate 0.58∼0.60중량%, protease peptone 1.16∼1.17중량%, beef extract 1.16∼1.17중량%, yeast extract 0.58∼0.60중량%, dextrose 2.34∼2.36중량%, magnese sulfate 0.0058∼0.0060중량%, polysorbate 93.67∼93.69중량%, magnesium sulfate 0.010∼0.015중량%, dipotassium phosphate 0.230∼0.235중량%를 포함하여 이루어지며,
상기 성분들이 포함된 Lactobacilli MRS 100중량부를 기준으로 Agar 1.5∼2.5중량부를 더 포함하여 구성됨을 특징으로 하는 유산균 발효 다시마추출물이 함유된 한방환의 제조방법.
According to paragraph 2,
In the shaking culture (S4) step, the Lactobacilli MRS contains 0.23 to 0.25% by weight of ammonium citrate, 0.58 to 0.60% by weight of sodium acetate, 1.16 to 1.17% by weight of protease peptone, 1.16 to 1.17% by weight of beef extract, and 0.58 to 0.60% by weight of yeast extract. % by weight, dextrose 2.34 to 2.36 wt%, magnesium sulfate 0.0058 to 0.0060 wt%, polysorbate 93.67 to 93.69 wt%, magnesium sulfate 0.010 to 0.015 wt%, and dipotassium phosphate 0.230 to 0.235 wt%. Lose,
A method for producing an herbal pill containing lactic acid bacteria-fermented kelp extract, characterized in that it further comprises 1.5 to 2.5 parts by weight of Agar based on 100 parts by weight of Lactobacilli MRS containing the above ingredients.
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