KR20240041518A - Method for cultivating bean sprouts with enhanced functionality and its system - Google Patents

Abstract

The present invention relates to a method and system for growing functional bean sprouts, and more specifically, to a first step of selecting bean sprouts and then washing them with ozonated water and removing moisture; A second step of soaking the washed bean sprouts in ozonated water and general sterilized water to soak the beans; A third step of producing cultivated water in which chitosan, selenium (Se), and calcium (Ca) are dissolved and lactic acid bacteria are added; A fourth step of cultivating bean sprouts by cross-spraying cultivation water and ozonated water; This relates to a method and system for cultivating bean sprouts with enhanced functionality, which includes the fifth step of washing the cultivated bean sprouts with ozonated water, removing moisture, and packaging them.
[index word]
Functional bean sprouts, bean sprout cultivation system, ozonated water, water treatment system

Description

Method for cultivating bean sprouts with enhanced functionality and its system {Method for cultivating bean sprouts with enhanced functionality and its system}

The present invention relates to a method of cultivating bean sprouts with enhanced functionality and a system thereof, specifically comprising the steps of selecting raw soybeans and washing them with micro bubbles in ozonated water; A step of soaking the raw soybeans by first immersing them in ozonated water and then immersing them in general sterilized water; Creating cultivation water for bean sprouts in which chitosan, selenium (Se) and calcium (Ca) are dissolved and lactic acid bacteria are added; Cultivating bean sprouts by cross-spraying soaking water and cultivation water; This relates to a method and cultivation system for cultivating bean sprouts with enhanced functionality that improves storage during the distribution process by washing the cultivated bean sprouts with ozonated water.

Soybeans contain all three major nutrients (41.3% protein, 26.1% carbohydrates, and 17.6% lipids), but they are lacking in minerals. Bean sprouts are grown in high humidity and dark conditions, and large amounts of beans are piled up in layers to germinate. As a result, a lot of respiratory heat is generated, and the temperature inside the cultivation container rises due to the respiratory heat, which leads to the proliferation of microorganisms such as Baciiius subtilis, Escherichia coli, Pseudomonas aeruginosa, and Fusarium sp. There is a problem that occurs actively and causes bean sprouts to rot.

Therefore, Patent Document 1 shortens the cultivation period of bean sprouts by about 1 day and prevents rot and deterioration of bean sprouts during the cultivation process by cross-irrigating the cultivation water at appropriate and low temperatures, and Patent Document 4 uses plasma electrodes. Put it in the water, sterilize the cultivation water by submersing the discharge gas for 3 to 7 minutes at a rate of 0.5 to 50 L/min, and reuse the cultivation water. Due to the reuse of the cultivation water, the bean sprouts are discharged into the cultivation water during growth. It is said that the growth of bean sprouts can be improved by using various active ingredients.

In addition, in order to enhance the functionality of bean sprouts, Patent Document 2 discloses pollution-free bean sprouts with improved calcium and nutrients using a peptide binding solution, and a cultivation method thereof, which involves the cultivation of bean sprouts with improved calcium using a peptide binding solution. As a method, Patent Document 3 discloses a method of cultivating calcium-fortified bean sprouts using calcium-fortified bean sprouts and starfish, in which calcium is fortified in bean sprouts using starfish as a natural calcium material.

In addition, in order to strengthen selenium (Se), Patent Document 5 discloses a selenium-enhanced bean sprout processing method, which increases the organic selenium (Se) content by absorbing the Na salt of the inorganic selenium (Se) compound into the bean sprouts and promotes the growth of bean sprouts. After all of this is completed, cultivation is completed using the time when absorption is strong, and the roots of the bean sprouts just before shipment are immersed in a high concentration selenium (Se) solution for several tens of minutes to dry the selenium (Se)-enhanced bean sprouts and use them as processing materials. It is for.

However, the present invention increases the germination rate of soybeans by taking advantage of the characteristic that during the process of soybean germination and growth into bean sprouts, decomposition metabolism mainly occurs in the cotyledons and synthetic metabolism occurs in the hypocotyl, so that the nutritional components of the bean sprouts are different from those of the soybeans. , provides a cultivation method and system for bean sprouts that increase the growth rate, enhance various functional substances in the cultivation water, and adjust the cultivation environment to enhance the functionality of bean sprouts as they grow into bean sprouts.

In addition, the environment such as cultivation temperature, irrigation amount, irrigation method, irrigation time, water quality, etc. of bean sprouts affect the yield and occurrence of rot of bean sprouts.

Therefore, in the present invention, since it is important to maintain a sterile condition in the cultivation of bean sprouts, the overall process of bean sprout production and the cultivation system, from selection, washing, and sterilization of the raw soybeans, cultivation of bean sprouts, and washing and packaging of bean sprouts, are maintained in a sterile state. We provide a cultivation method and system for bean sprouts with enhanced functionality that fundamentally blocks rot and deterioration, which are the most problematic problems in bean sprout cultivation, increases the germination rate, increases self-production of functionality during the growth process of bean sprouts, and increases yield.

Republic of Korea Patent Registration No. 10-1042335 Bean Sprout Cultivation Method (June 10, 2011) Republic of Korea Patent Registration No. 10-2067480 Pollution-free bean sprouts with improved calcium and nutrients using peptide bond solution, and their cultivation method (January 13, 2020) Republic of Korea Patent Registration No. 10-0586704 Calcium-fortified bean sprouts and cultivation method of calcium-fortified bean sprouts using starfish (May 29, 2006) Republic of Korea Patent Registration No. 10-1782565 Bean sprout cultivation method using plasma discharge water (September 21, 2017) Republic of Korea Patent Registration No. 10-0558385 Selenium-enhanced bean sprout processing method (February 28, 2006)

The present invention solves all the problems of the prior art as described above, and was created with an advanced technology different from existing patents. It automatically cleans the spirit of the raw material, bean sprouts, and cleans it with micro bubbles in an ozone water tank. Washed with micro bubbles, immersed in ozone water that promotes germination and inhibits the growth of spoilage microorganisms, and saponin, isoflavone, and gamma-aminobutyric acid produced during the growth process of bean sprouts. : Promotes functional metabolism such as GABA), manufactures cultivation water that enhances calcium (Ca) and selenium (Se), and develops a method and system for cultivating functionally enhanced bean sprouts grown with functionally enhanced cultivation water. to provide.

The bean sprout cultivation method and system with enhanced functionality of the present invention for achieving the above object include a washing step of selecting bean sprouts, washing them with micro bubbles in an ozone water tank, and removing moisture; It is characterized by first immersing washed raw soybeans in ozone water, which can promote germination and inhibiting the growth of putrefactive microorganisms, and secondly immersing them in general sterilized water to soak the soybeans to promote germination and prevent spoilage.

In addition, the bean sprout cultivation water is prepared by dissolving chitosan, calcium (Ca), and selenium (Se) in sterilized water, and the cultivation water prepared by adding lactic acid bacteria bred by the present inventor is cross-sprayed with ozonated water to cultivate bean sprouts. It is characterized by

In addition, it is characterized by being equipped with an ozonated water production device capable of quickly producing the ozonated water, maintaining all cultivation environments of the bean sprouts in a sterile state, and automatically controlling all operations.

In addition, the cultivation container is equipped with an open bottom so that irrigation water drains out, and the cultivation container is placed on a cultivation bed equipped with a shelf to fundamentally block contamination from the bottom.

In addition, the cultivation bed is equipped with a shelf, so that the cultivation container can be placed on the cultivation bed to make wide use of the narrow cultivation area. The bottom of the cultivation bed is sealed, and the sealed floor is equipped with a drain that collects and discharges the cultivation water that drains from the cultivation container to one place. By supplying sterilized air with ozone (O ₃ ) between the sealed bottom of the bed and the cultivation container above the cultivation bed, it suppresses the rise in temperature of bean sprouts during cultivation, supplies oxygen (O ₂ ), and prevents the growth of putrefactive bacteria. It is characterized by

The present invention has the effect of improving health through simple food consumption of bean sprouts with improved quality, improved productivity, and enhanced various functionality, and increases the income of farmers through pesticide-free and clean bean sprouts produced in sterile conditions in all processes.

Figure 1 is a flow chart for cultivating bean sprouts according to the present invention.
Figure 2a is a chart showing the electron spin resonance spectrum of micro ozone (O3) bubbles and micro air bubbles discharged into distilled water for 20 seconds, Figure 2b is a table showing bubble names according to the size of the bubbles, and Figure 2c is a general air bubble. This is a picture showing the extinction process of micro bubbles.
Figure 3 is a conceptual diagram of a washing machine for raw soybeans of the present invention,
Figure 4a is a general ozonated water production facility, Figure 4b is an ozonated water production facility of the present invention, Figure 4c is a side view showing the diaphragm of the ozonated water production facility of the present invention, and Figure 4d is a view of the diffuser and the microbubbles generated from the diffuser. It's a photo.
Figure 5a is a graph showing the yield of bean sprouts when grown for 7 days with the cultivation water and ozonated water according to the present invention, and Figure 5b is a graph showing the crude ponin content in bean sprouts according to time during cultivation.
Figure 6a is a photograph of the external appearance of the container cultivation center of the present invention, Figure 6b is a photograph of a cultivation bed equipped with multiple shelves, and Figure 6C is a photograph of the piping at the bottom of the cultivation bed that collects cultivation water discharged from the material container.
Figure 7a is a photograph of a plastic cultivation container placed on a cultivation bed of the present invention, and Figure 7b is a photograph of ozone water and cultivation water being automatically sprayed into a plastic cultivation container.
Figure 8 is a diagram showing the length of bean sprouts according to the watering method used in the present invention,
Figure 9a is the base sequence of the lactic acid bacterium Lactobacillus plantarum EK1 developed by the present inventor, and Figure 9b is a classification table of the lactic acid bacterium Lactobacillus plantarum EK1.

Hereinafter, embodiments of the present invention will be described in detail so that those skilled in the art can easily implement the present invention.

The present invention may be implemented in many different forms and is not limited to the embodiments described herein.

In general, the biggest problem with bean sprout cultivation is that it continues to secrete sugar, protein, and mucus in the early stages of germination, and respiratory heat is generated during growth, leading to rot due to the proliferation of various pathogens and other microorganisms. In particular, bean sprout cultivation has high humidity and microorganisms. This favorable temperature for growth and the nutrients of soybeans provide all three conditions for microbial growth, moisture, nutrients, and temperature, which leads to a decrease in the production yield and quality of bean sprout cultivation and causes enormous losses during distribution. Pesticides are used to prevent this. There is an example of this.

In addition, another problem with bean sprout cultivation is that the germination rate of raw soybeans is low. The germination rate of raw soybeans is affected by the storage method and storage period of raw soybeans, and various requirements including cultivation water, cultivation environment, and irrigation conditions are affected. It is caused by changes in nutrients and functional substances due to respiration and metabolism during the germination process of raw soybeans, resulting in differences between the nutrients and functional substances of soybeans and those of bean sprouts.

Therefore, the bean sprout cultivation method and system with enhanced functionality of the present invention fundamentally prevents rot and deterioration of bean sprouts during the process of cultivating raw soybeans, increases the germination rate of soybeans, increases the saponin content that changes during the growth process, The isoflavone content is increased, Gamma-Aminobutyric acid (GABA) is produced, functional bean sprouts with enhanced selenium (Se) and calcium (Ca) are grown, and the entire cultivation process is automatically controlled to save labor. It relates to a method and system for cultivating bean sprouts with enhanced functionality that reduces production and increases productivity.

In order to implement this, the present invention includes a sorting device (100) for selecting good beans by selecting diseased beans and foreign substances from raw beans; A raw soybean washing device (200) that cleans the selected raw soybeans and simultaneously kills spoilage bacteria; an ozonated water production device 300 that supplies ozonated water to each process; A immersion tank 400 in which washed and sterilized raw soybeans are placed in water; A cultivation device (500) for germinating raw soybeans soaked in an immersion tank (400) and cultivating them into bean sprouts; A bean sprout washing device (600) for washing and sterilizing cultivated bean sprouts; It is provided with a packaging device (700) for packaging washed and sterilized bean sprouts in a sterile state.

The washing device 200 includes a cylinder 210 containing raw soybeans and sterilizing washing water, as shown in Figure 3; A door 220 equipped with a locking device 221 to insert and remove washing water and raw soybeans and prevent the contents from leaking out during rotation; a rotating shaft 230 provided as a pipe at the center of both sides of the cylinder 210 and rotating the cylinder 210; An ozone (O 3 ) supply pipe 240 provided on the inner lower wall of the cylinder 210 and provided with _{a plurality of fine holes through which ozone (O 3 )} is supplied; An ozone (O ₃ ) generator 250 that supplies ozone (O ₃ ) to the ozone (O ₃ ) supply pipe 240; It is characterized in that a driving device 260 is connected to the rotation shaft 230b on the other side and rotates the rotation shaft 230b.

In addition, the raw soybeans are washed and sterilized by ozone washing water and ozone (O ₃ ) aerated for 5 to 10 minutes through the ozone (O 3 ) supply pipe ₂₄₀ , and the aerated ozone (O ₃ ) As it passes through the ozone water in the cylinder 210, a large amount of OH ^- radicals are generated through a process of contraction and extinction, decomposing toxic and difficult-to-decompose substances, and the surface of the raw soybean is evenly cleaned and sterilized due to its high sterilizing power. .

In addition, the ozone (O ₃ ) supply pipe 240 is provided close to the inner lower wall of the cylinder 210, and the ozone (O ₃ ) generator 250 and the ozone (O ₃ ) supply pipe 240 are connected to each other. To this end, a connection pipe 241 is provided, and the connection pipe 241 is fixed to the outside of the cylinder 210 so that the connected ozone (O ₃ ) supply pipe 240 is fixed, so that even if the cylinder 210 is rotated, the connection pipe 241 is fixed to the outside of the cylinder 210. The ozone (O ₃ ) supply pipe 240 does not rotate, and blocks the flow of raw soybeans that are about to rotate according to the rotating cylinder 210, resulting in the effect of stirring the raw soybeans evenly and supplying them from the ozone (O ₃ ) supply pipe 240. The fine ozone (O ₃ ) rises to the top in the form of air bubbles and evenly contacts the surface of the raw soybeans through the mechanism shown in Figure 2, increasing the cleaning and sterilization effect.

In addition, washing water for raw soybeans with various sterilizing properties, such as chlorine dioxide water, electrolytic oxidation water, chlorine water, electrolytic water, and electric discharge water, is generally used. The washing method involves soaking raw soybeans in such washing water, It is cleaned and sterilized by stirring with a spatula or shovel.

However, the washing water of the present invention has 6 to 7 times the oxidizing power and 20 times the sterilizing power compared to chlorine (Cl ₂ ), and does not generate additional by-products, so it is recognized as an eco-friendly sterilization method. Unlike chlorine-based disinfectants, it does not leave any residual substances after treatment. Ozone water, which does not cause secondary pollution, is used as the washing water, and the washing method uses the washing device 200 as shown in FIG. 3, so the washing labor is reduced, the washing is fast, and the washing is evenly cleaned. It is characterized by increasing the sterilization effect.

The ozonated water production device 300 includes a diffuser 310 that generates micro bubbles of 50㎛ or less as shown in FIG. 4; A blower 320 that blows ozone (O ₃ ) generated from the ozone (O ₃ ) generator 250 to the diffuser 310; A diaphragm 330 provided with irregularities like an egg carton to retain ozone (O ₃ ) in the water and increase the dissolution rate of ozone (O ₃ ) dissolved in the water; It is characterized by rapidly producing ozonated water by increasing the number of diaphragms 330.

The immersion tank 400 includes a water supply device 410 that fills water; A drainage device 420 for removing water; The immersion tank 400 is equipped with a micro bubble supply device 430 that supplies micro bubbles of 50㎛ or less, and the water supply device 410 includes a water pump 411; Water supply pipe 412; It is equipped with a solenoid valve (413) that supplies and blocks water, and the drainage device (420) includes a drainage pump (421); Drain pipe 422; It is equipped with a solenoid valve (423) that drains and blocks water, and the micro bubble supply device (430) includes a diffuser (431) provided at the bottom of the immersion tank (400); It is characterized in that a blower 432 that blows sterilized air is provided in the diffuser 431.

The immersion of the raw soybeans in the immersion tank 400 is to soak the raw soybeans in order to promote germination by supplying moisture to the raw soybeans. In ozone water with an ozone (O ₃ ) concentration of 0.25 to 0.3 ppm at 16 to 20 ° C, 40 a first soaking step of ~50 minutes; By immersing in sterilized general water at 16~20℃ for 5~8 hours, the germination rate of raw soybeans is high, the yield of bean sprouts, hypocotyl weight, average length, and root weight are high, and the decay rate is low. It is characterized by

The bean sprout cultivation device 500 includes a grower 510 for cultivating bean sprouts; A cultivation bed (520) provided with multi-level shelves (521); A cultivation container 530 for cultivating bean sprouts containing soaked raw soybeans, and a spray device 540 for automatically watering the cultivation container 530; It is equipped with heating equipment (550) and cooling equipment (560) that can control the cultivation room (510) and the water temperature required for cultivation, and is equipped with a ventilation port (570) and an intake port (580) that can ventilate indoor air. Do it as

The bean sprout cultivation bed 520 is equipped with multi-level shelves 521 to accommodate many cultivation containers 530, as shown in FIGS. 6 and 7, allowing a large amount of bean sprouts to be grown in a small area, Each shelf 521 is provided with a frame 522 and a floor 523. The upper frame 522 is provided with a spray nozzle 541 as shown in Figure 8b, and the floor 523 is provided in the form of a sealed box. , a drain 525 is provided on one side of the floor 523 to collect the received ozonated water and cultivation water, and a water supply port 526 is provided on the other side to supply sterilized air.

In addition, as shown in Figure 7b, the spray nozzle 541 provided at the top of each shelf 521 is provided to spray water downward on the pipe, and the water supply pipe is supplied with an electromagnetic valve (soienoia vaive: 527). It opens and closes alternately to automatically supply ozonated water and cultivation water, and the pipe equipped with the spray nozzle 541 is equipped to automatically move back and forth forward and backward.

In addition, the bean sprout cultivation container 530 is preferably made of plastic, the top is open, the sides are closed, and the bottom has a large number of small holes large enough to prevent the soaked raw beans from falling out, and is sprayed evenly on the bottom. The ozonated water and the cultivation water are provided to be discharged naturally, and the sterilized air supplied between the sealed bottom 523 of the shelf 521 and the cultivation container 530 passes through the bottom hole of the cultivation container 530, thereby providing the respiratory heat of the bean sprouts during the cultivation process. By cooling the bean sprouts, the temperature of the bean sprouts increases during cultivation, thereby preventing the propagation of spoilage bacteria. It also functions to supply oxygen to the bean sprouts, suppressing the development of fine roots, and producing high-quality bean sprouts.

In the bean sprout cultivation, many cultivation containers 530 are placed on a multi-level shelf 521 provided in a dark cultivation room 510 blocked from sunlight, as shown in FIG. 6b, and the cultivation water is cultivation water and ozone water at 21 to 23 ° C. Cross-spraying is performed, and the spraying method is characterized by cross-spraying at 3-4 hour intervals for 1-5 minutes at a time for 5-7 days.

In the bean sprout cultivation, improving the decay rate and germination rate of the raw material beans and the decay rate and growth of the bean sprouts are important factors for the success of bean sprout cultivation, and it is very important to solve these problems.

Therefore, in order to increase the germination rate of raw soybeans, as shown in Table 1, ozone (O ₃ ) is naturally decomposed within 20 minutes at the bean sprout cultivation temperature of 21-23°C, and ozone (O) is decomposed as shown in Chemical Formulas (1) and (2). ₃ ) By utilizing oxygen (O ₂ ), the germination rate of raw soybeans is increased by supplying oxygen (O ₂ ), and oxygen (O ₂ ) is supplied to growing bean sprouts to enhance the growth of bean sprouts. do.

O ₃ + OH- → HO ₂ - + O ₂ ................... (1)

O ₃ + HO ₂ - → ·OH + O ₂ ·- + O ₂ ............ (2)

Table 1 Ozone decomposition rate of ozonated water (pH 7)

In addition, the World Health Organization (WHO)'s ozone (O ₃ ) disinfection standard sets the standard for maintaining a concentration of 0.4 ppm or higher for at least 4 minutes, but the optimal ozone (O ₃ ) of ozone water for immersing raw soybeans in the cultivation of bean sprouts is In order to confirm the concentration, ozone water immersion time, and optimal ozone (O ₃ ) concentration of ozonated water sprayed for bean sprout cultivation, the same experiment as Example 1 was performed.

Effect of ozone (O ₃ ) concentration and ozone water immersion time on bean sprout cultivation

In general, the biggest problems in growing bean sprouts are the germination of raw soybeans and the decay of bean sprouts. Therefore, methods to increase the germination rate of raw soybeans, prevent spoilage of bean sprouts should be considered, and increase the growth rate of bean sprouts. Several factors were investigated through experiments.

The present invention used ozone (O ₃ ), which does not generate new secondary products to prevent corruption, and used an ozone (O ₃ ) generator from WooSung Hi-Thec Co., SP-100, Korea, and ozone (O ₃ ) ) Concentration was measured using a monitor (Ebara Jitsugyo Co., PL-320, Japan).

The present invention was designed using a central synthesis method to find the optimal ozone (O ₃ ) concentration for immersing raw soybeans in ozonated water. As a result of finding the optimal conditions using response surface analysis, the ozone (O ₃ ) concentration was set to 0.25 to 0.3 ppm, The ozonated water treatment time is immersion in ozonated water for 40-50 minutes, soaked raw soybeans in general sterilized water at 16-20℃ for 5-8 hours, and 0.3-0.5ppm ozonated water at about 20℃ and cultivation water every 3-4 hours. As a result of spraying and cultivation for 5 days, the germination rate increased by 16%, the hypocotyl weight increased by more than 69%, and the root weight decreased by more than 98% compared to the untreated group, as shown in Table 2.

According to the results of Example 1, raw soybeans were immersed in ozone water with an ozone (O ₃ ) concentration of 0.25 to 0.3 ppm for 40 to 50 minutes, then placed in water at 16 to 20°C and soaked for 5 to 8 hours. Put it in a cultivation box and spray it with 0.3-0.5 ppm ozone water adjusted to 21-23℃ and cultivation water mixed with chitosan, selenium, calcium, and microorganisms at 3-4 hour intervals for 1-5 minutes each time for 5 days. This prevents rotting of raw soybeans, increases germination rate, prevents rotting of bean sprouts, improves yield, and enhances various functionalities.

In addition, the same experiment as Example 2 was performed to confirm the chitosan content of the cultivation water sprayed for bean sprout cultivation.

Table 2 Germination rate and bean sprout weight and length comparison table when sprayed with ozonated water (% compared to control)

Effect of chitosan on changes in saponin content of bean sprouts

To confirm the effect of chitosan, which has not yet been identified among the secondary metabolites closely related to the growth resistance of bean sprouts, on changes in saponin content of bean sprouts, the growth rate of bean sprouts grown by treating chitosan was observed, and these were treated at different concentrations. The crude ponin content of bean sprouts was measured and compared with the control group not treated with chitosan.

Therefore, the calibration curve of crude soybeans, confirmed by Liberman-Burchard color reaction using roughasponin purchased from Wako as a standard, was Y=911.22X-0.033, the correlation coefficient (R ² ) was 0.998, and the Saponin was 4.59 mg/g, and after immersion in water for 5 hours, saponin was eluted into the immersion solution and decreased to 4.18 mg/g. As the soybeans germinated and grew, it gradually increased to 5.30-5.33 on the 5th to 6th day of cultivation. It was highest at mg/g, but the saponin content decreased on the 7th day.

In addition, the saponin content of each part during the growth of bean sprouts was 4.17 mg/g in the cotyledons, 7.46 mg/g in the stem, and 7.45 mg/g in the root, which was about twice higher in the stem and root than in the cotyledon. , the contents of stems and roots were similar.

In addition, in order to treat chitosan to bean sprouts, 30 CPS (M.W. = 75,000) of chitosan was purchased and used from Biotech Co., Ltd., and the deacetylation degree was 89%. The chitosan solution contained a small amount of chitosan to achieve concentrations of 250 ppm, 500 ppm, 1000 ppm, and 2000 ppm. After adding lactic acid and completely dissolving it, it was prepared by adjusting the pH to 6.0 with sodium hydroxide (NaOH). The control group was untreated with chitosan. The control group was also adjusted to pH 6.0, and bean sprouts were grown in the same manner as in the above experiment. They were grown for one day, harvested, and changes in the yield of bean sprouts were observed.

As a result, the control group was 18.57%, and in the chitosan-treated group, the 250ppm treatment group was 25.29%, the 500ppm treatment group was 25.54%, the 1000ppm treatment group was 21.14%, and the 2000ppm treatment group was 19.86%. The 500ppm treatment group was the highest at 25.54%, and the control group had 25.54%. Compared to the other groups treated with chitosan, the values were all higher.

The crude ponin content of bean sprouts was 7.96±0.29 mg/g in the 250ppm chitosan treatment group, 8.15±0.39 mg/g in the 500ppm treatment group, 7.61±0.22 mg/g in the 1000ppm treatment group, and 7.52±1.29 mg/g in the 2000ppm treatment group. The crude ponin content was the highest in bean sprouts, and was significantly (p<0.05) higher than the control.

In addition, to investigate the growth characteristics of bean sprouts treated with chitosan and grown for 5 days, the germination rate, yield, weight of hypocotyl and cotyledon, and thickness and length of hypocotyl were observed. As a result, bean sprouts treated with chitosan had the highest germination rate, yield, and per individual. Weight, weight of hypocotyl and cotyledons, and thickness of hypocotyl were significantly higher than that of the control.

Specifically, the germination rate of bean sprouts grown for 5 days was 69 to 85% in the control group and 88 to 94% in the chitosan treatment group, which was 10.5 to 27.5% higher than the control group. The yield was 638.6% in the control bean sprouts and the chitosan treatment group was 638.6%. It was 702.8%, 10% compared to the control, and the weight of bean sprouts per individual in the control was 0.75g and the chitosan treatment was 0.79g, which was 0.04g heavier in the chitosan treatment than in the control, and the weight of the cotyledons was 2.5 to 2.7 in the control. g, the weight of the treatment group was 2.3~2.6g, which was 3.8~8.7% higher in the control group, and the weight of the hypocotyl was 0.9~3.6g in the control group, and 1.5~4.5g in the treatment group, which was 25~66.7% higher in the treatment group, and the weight of the hypocotyl was 25~66.7% higher in the control group. The length of the control group was 16.51 to 19.87 cm, and the treatment group was 17.67 to 22.38, which was 7 to 12.6% higher. The hypocotyl thickness was 1.89 mm in the control group and 2.03 mm in the chitosan treatment group, which was 7.4% higher in the treatment group. .

Therefore, as in Example 2, the chitosan solution is diluted to 200 to 300 ppm in sterilized water to increase the germination rate of bean sprouts, increase the saponin content of bean sprouts, and increase the productivity of bean sprouts.

In addition, the same experiment as in Example 3 was performed to confirm the selenium (Se) content of the cultivation water sprayed for bean sprout cultivation.

Effect of selenium (Se) fortification on bean sprouts

According to recent research on selenium (Se), monomethylated selenium metabolites are known to be crucial in preventing cancer, and all selenium (Se) contained in grains is organically bound selenium (Se), with more than half of it being selenomethionine (Se). It exists in the form of Se-methionine).

Therefore, in order to enhance selenium (Se) in the bean sprouts of the present invention and to investigate the content of organically bound selenium (Se) in the bean sprouts cultivated in this way, selenium (Se) was added to sterilized water at 0, 1, 3, 5, and 7, respectively. , an aqueous solution of selenium (Se) was prepared to a concentration of 10ppm, and this was used as bean sprout cultivation water while maintained at 21-23°C. This cultivation water was sprayed for 1-5 minutes once at 3-4 hour intervals for 5 days. Bean sprouts were grown, and the total content of selenium (Se) contained in the bean sprouts was pretreated as shown in Figure 9 and analyzed using ICPMS.

As a result, the control group without adding selenium (Se) contained about 1.182 ppb selenium (Se), and the group treated with 1 ppm selenium (Se) contained 5.108 ppb selenium (Se), about 4 times more than the control group. The 3ppm treatment group showed a 14-fold increase in selenium (Se), and the 5ppm treatment group showed a 30-fold increase in selenium (Se). However, high-concentration selenium (Se) solutions of more than 5ppm resulted in reduced growth of bean sprouts. The degree of selenium (Se) reinforcement does not increase significantly compared to the treatment with 5ppm or less, and some show a tendency to decrease, so treatment with 5ppm or less is preferable.

Table 3 Concentration of selenium (Se) aqueous solution and selenium content of bean sprouts

Selenium (Se), which shows a high strengthening phenomenon in bean sprouts, is an important active ingredient of glutathione peroxidase, an antioxidant enzyme. As in Example 3, the aqueous selenium (Se) solution for strengthening selenium (Se) has a pH of 6.5~7.5 It is diluted to 1~5ppm in sterilized water and is characterized by enhancing selenium (Se) in bean sprouts by up to 30 times.

In addition, it is a flavonoid-based substance that has a similar structure and activity to the female hormone estrogen, increasing the isoflavone content, also known as phytoestrogen, and having the ability to produce Gamma-Aminobutyric acid (GABA) 1.4. It is characterized by using ×10 ¹⁰ CFU/g of Lactobacillus plantarum EK1 ( Lactobacillus plantarum EK1).

Therefore, in order to increase the saponin content as the bean sprouts grow, chitosan solution is diluted to 200 to 300 ppm in the cultivation water, and to strengthen calcium (Ca), selenium (Se) aqueous solution is diluted to 0.1 to 1%. In addition, to strengthen selenium (Se), a selenium (Se) aqueous solution was added diluted to 1 to 5 ppm, and 1.4 × 10 ¹⁰ CFU/g Lactobacillus plantarum EK1 ( Lactobacillus plantarum EK1) was used as cultivation water. It is characterized by:

In order to confirm the effect of cultivation temperature and cultivation water temperature on bean sprout cultivation during the bean sprout cultivation process, the same experiment as in Example 4 was performed.

Effect of cultivation temperature and cultivation water temperature on bean sprout cultivation

If the cultivation temperature is low, the bean sprouts will not rot, but if the cultivation temperature is high, the growth of the bean sprouts will be slow, and if the cultivation temperature is high, the sprouts will rot, so it is important to cultivate them at the optimal temperature. The growth of bean sprouts according to the cultivation temperature is important. As a result of investigating the degree of corruption, there was no corruption at 20℃, and the results are shown in Table 2.

Table 2. Degree of spoilage of bean sprouts according to temperature

In addition, in order to check the growth and shape changes of bean sprouts when the cultivation room temperature was changed, the cultivation room temperature was varied during the cultivation period, and the growth and germination levels of the bean sprouts were classified by hypocotyl length, and the cultivation room temperature was set to 22°C. When maintained, the percentage of hypocotyl length of 7 cm or more, which is the most marketable, was the highest, and the weight was also high for each part, but the frequency of appearance of black spots was high, which lowered marketability, and the results are shown in Table 3.

In addition, bean sprouts are prone to rot when the water temperature of the cultivation water is above 23℃, and when it is above 17℃, rot does not occur, but the growth of bean sprouts is delayed due to the difference between the cultivation temperature and the water temperature of the cultivation water, making bean sprout cultivation difficult. Cultivating bean sprouts with the cultivation temperature and cultivation water temperature maintained uniformly around 20°C can reduce the occurrence of rot, and the results are shown in Table 4.

Table 3. Growth and germination level of bean sprouts according to temperature changes in the cultivation room

Table 4. Degree of rotting of bean sprouts according to cultivation water temperature

In addition, the development of fine roots and the thickness and length of the hypocotyl have a significant impact on the product of bean sprouts. However, in order to produce pesticide-free and clean bean sprouts, this problem must be solved in the cultivation method, and as a solution, cultivation by using different watering methods is required. did.

The interval and time of watering the bean sprouts is watered for 3 minutes every 3 hours from the first day to the 3rd day, and from the 4th day when the bean sprouts have grown to a certain degree, watered for 4 minutes every 2 hours to provide sufficient moisture, the ratio of the hypocotyls of the bean sprouts increases. It increases by 54-65%, increasing the marketability of bean sprouts.

For the above bean sprout cultivation, the cultivation room temperature is set at 20~23℃, the water temperature of the cultivation water is set at 20~23℃ for both ozone water and cultivation water, and the spraying method is 5~5 minutes at 3~4 hour intervals for 1~5 minutes at a time. Cross-spraying for 7 days prevents rot, improves growth, and increases the marketability of bean sprouts.

The automatic washing machine 600 for washing the cultivated bean sprouts is equipped with an air diffuser 620 at the bottom of the washing tank 610 for washing the bean sprouts, and micro bubbles with a diameter of 10 to 50 μm are generated through the air diffuser 620. As the micro bubbles are aerated and rise, they prevent the bean sprouts being cleaned from sinking to the bottom. The micro bubbles evenly contact the surface of the bean sprouts, cleaning them without breaking them, and sterilizing them with the high sterilizing power of OH ^- (OH radical). It is characterized by increasing the storability of bean sprouts after packaging and during distribution.

The packaging device 700 includes an automatic vinyl packaging machine 710; It is equipped with an automatic box packaging machine (720), and the washed bean sprouts are weighed and packaged in plastic wrappers (711). The bean sprouts packed in plastic wrappers (711) are placed in a certain amount in a packaging box (721), and are then packaged and shipped. , This entire process is characterized by being sterile.

Although specific embodiments of the bean sprout cultivation method and system with enhanced functionality of the present invention have been described, it is obvious that various implementation modifications are possible without departing from the scope of the present invention.

Therefore, the scope of the present invention should not be limited to the described embodiments, but should be determined by the claims and equivalents thereof as well as the claims described later.

That is, the above-described embodiments should be understood in all respects as illustrative and not restrictive, and the scope of the present invention is indicated by the claims to be described later rather than the detailed description, and the meaning and scope of the claims and All changes or modified forms derived from the equivalent concept should be construed as falling within the scope of the present invention.

100: Sorting device
200: raw soybean washing device 210: cylinder
220: door 221: lock
230: Rotating shaft 240: Ozone (O ₃ ) supply pipe
241: Connection pipe 250: Ozone (O ₃ ) generator
260: driving device
300: Ozonated water production device 310: Diffuser
320: blower 330: diaphragm
400: Immersion tank 410: Water supply device
411: water pump 412: water supply pipe
413: solenoid valve
420: Drainage device 421: Drainage pump
422: drain pipe 423: solenoid valve (soienoid valve)
430: Micro bubble supply device 431: Diffuser
432: Blower
500: Cultivation device 510: Cultivation plant
520: cultivation bed 521: multi-level shelf
522: Frame 523: Floor
525: Drain 526: Supply port
527: soienoid valve
530: Cultivation container 540: Spray device
541: spray nozzle 550: heating equipment
560: Cooling equipment 570: Ventilation opening
580: intake port
600: Bean sprout washing device 610: Washing tank
620: diffuser
700: Packaging device 710: Automatic vinyl packaging machine
711: Plastic wrapping paper 720: Automatic box packaging machine
721: Packaging box

Claims (12)

A sorting device that selects good soybeans by selecting diseased soybeans and foreign substances from raw soybeans;
A raw soybean washing device that cleans the selected raw soybeans and simultaneously kills spoilage bacteria;
An ozonated water production device that supplies ozonated water to each process;
A soaking tank in which washed and sterilized raw soybeans are placed in water;
A cultivation device for germinating soaked raw soybeans and cultivating them into bean sprouts;
A bean sprout washing device for washing and sterilizing cultivated bean sprouts;
A bean sprout cultivation method and system with enhanced functionality, characterized in that washed and sterilized bean sprouts are packaged in a sterile state. According to clause 1,
The washing device is
A cylinder containing raw beans and sterilizing washing water;
A door equipped with a locking device to insert and remove washing water and raw soybeans and prevent the contents from leaking out during rotation;
a rotating shaft provided as a pipe at the center of both sides of the cylinder to rotate the cylinder;
An ozone (O ₃ ) supply pipe 240 provided on the inner lower wall of the cylinder and having a plurality of fine holes through which ozone (O ₃ ) is supplied to the raw soybeans and the sterilizing washing water;
An ozone (O ₃ ) generator 250 that supplies ozone (O ₃ );
A method and system for growing bean sprouts with enhanced functionality, characterized in that it is provided with a driving device that is connected to the rotating shaft on the other side and rotates the rotating shaft. According to clause 2,
Cleaning of the cleaning device is
The ozone (O ₃ ) supply pipe blocks the flow of raw soybeans that are about to rotate along the rotating cylinder, resulting in the effect of stirring the raw soybeans evenly, and the ozone (O ₃ ) supplied through the ozone (O ₃ ) supply pipe into the ozone washing water in the cylinder. A method and system for growing bean sprouts with enhanced functionality, characterized in that a large amount of OH ^- radicals with high sterilizing power are generated while aerated for 5 to 10 minutes and are washed and sterilized while evenly contacting the surface of the raw soybeans. According to clause 2,
The washing water is
Compared to chlorine (Cl ₂ ), the oxidizing power is 6 to 7 times higher, and the sterilizing power is more than 20 times higher. No additional by-products are generated, so there is no secondary pollution. Ozone water, which has high sterilizing power, is used as washing water to improve cleaning and sterilizing effects. A method and system for growing bean sprouts with enhanced functionality, characterized by increased functionality. According to clause 1,
The ozonated water production device is
an ozone water tank;
Micro bubble ozone (O ₃ ) stays in the water to increase the dissolution rate of ozone (O ₃ ), which is provided with a diaphragm that is uneven like an egg carton,
A method and system for growing bean sprouts with enhanced functionality, characterized in that ozonated water can be quickly created by simply increasing the number of diaphragms. According to clause 1,
The immersion tank is equipped with a micro bubble supply device that supplies micro bubbles of 50㎛ or less to provide a stirring effect for the raw soybeans during immersion, and the ozone (O ₃ ) of the ozone water in the immersion tank is oxygen. A bean sprout cultivation method and system with enhanced functionality characterized by increasing the germination rate by changing to (O ₂ ). According to clause 6,
Soaking of the raw soybeans
A first immersion step in which raw soybeans are immersed in ozone water with an ozone (O ₃ ) concentration of 0.25 to 0.3 ppm at 16 to 20°C for 40 to 50 minutes; The secondary immersion step of immersion in sterilized general water at 16-20℃ for 5-8 hours is continuous, resulting in a high germination rate of raw soybeans, improved yield of bean sprouts, and low decay rate. Bean sprout cultivation method and system. According to clause 1,
The bean sprout cultivation device is
A cultivation bed equipped with multi-level shelves so that many cultivation containers can be placed in a narrow space;
On one side of the bottom of each sealed cultivation bed is a drain for collecting and discharging cultivation water;
The other side of the bottom of the cultivation bed is equipped with a supply port that supplies sterilized air to cool the respiratory heat of bean sprouts growing in the standing cultivation container, increase the yield of bean sprouts by supplying oxygen (O ₂ ), and promote the development of fine roots of bean sprouts. A method and system for cultivating bean sprouts with enhanced functionality, characterized in that they produce high-quality bean sprouts by inhibiting the production of high-quality bean sprouts. According to clause 8,
The cultivation water is
To prevent rot and deterioration of bean sprouts and to increase the yield and functional substances of bean sprouts, ozonated water at 21-23°C;
A method and system for growing bean sprouts with enhanced functionality, characterized in that cross-spraying cultivation water enriched with functional substances for 1 to 5 minutes at a time at 3 to 4 hour intervals for 5 to 7 days. According to clause 9,
The ozone water in the above cultivation water is
The ozone (O ₃ ) concentration is set to 0.3 to 0.5 ppm, and the water temperature is set to 21 to 23°C.
Cultivated water
chitosan, which increases the yield of bean sprouts and the saponin content of bean sprouts;
Selenium (Se) and calcium (Ca);
Enhanced functionality, characterized by using a mixture of one or more Lactobacillus plantarum EK1 ( Lactobacillus plantarum EK1), which enhances functional substances such as isoflavone and gamma-aminobutyric acid (GABA). Bean sprout cultivation method and system. According to clause 9,
Chitosan in the cultivation water is added to sterilized water by diluting the chitosan solution to 200-300 ppm,
Selenium (Se) is diluted to 1 to 5 ppm in sterilized water with a pH of 6.5 to 7.5.
Calcium (Ca) is added diluted to 0.1 to 1% in calcium (Ca) aqueous solution.
Bean sprout cultivation with enhanced functionality, characterized by using 1.4 × 10 ¹⁰ CFU/g Lactobacillus plantarum EK1 to produce isoflavone and gamma-aminobutyric acid (GABA). Method and its system. According to clause 1,
The automatic washing machine that cleans the bean sprouts whose cultivation has been completed is
a washing tank containing ozonated water as washing water;
A diffuser provided at the bottom of the washing tank;
Micro ozone (O ₃ ) bubbles with a diameter of 10 to 50㎛ are aerated through the diffuser and rise to prevent _the bean sprouts being washed from sinking to the bottom. A method and system for growing bean sprouts with enhanced functionality, which includes washing the bean sprouts so that they do not break while contacting the surface evenly, and sterilizing them with the high sterilizing power of OH ^- (OH radical) to increase the storability of the bean sprouts after packaging and during distribution.