KR20240019120A - 바이러스 벡터 생산 시스템, 바이러스 벡터 생산을 위한 조작된 세포 및 이의 사용 방법 - Google Patents

바이러스 벡터 생산 시스템, 바이러스 벡터 생산을 위한 조작된 세포 및 이의 사용 방법 Download PDF

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Publication number
KR20240019120A
KR20240019120A KR1020237042063A KR20237042063A KR20240019120A KR 20240019120 A KR20240019120 A KR 20240019120A KR 1020237042063 A KR1020237042063 A KR 1020237042063A KR 20237042063 A KR20237042063 A KR 20237042063A KR 20240019120 A KR20240019120 A KR 20240019120A
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South Korea
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nucleic acid
acid sequence
viral vector
vector production
expression cassette
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Pending
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KR1020237042063A
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English (en)
Korean (ko)
Inventor
제레미 제이. 갬
크리스토퍼 에스. 스타키
알렉 에이. 케이. 닐슨
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아시모브 인코포레이티드
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2740/00—Reverse transcribing RNA viruses
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    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/15051—Methods of production or purification of viral material
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16041—Use of virus, viral particle or viral elements as a vector
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    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/16011—Human Immunodeficiency Virus, HIV
    • C12N2740/16051—Methods of production or purification of viral material
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    • C12N2750/14011—Parvoviridae
    • C12N2750/14041—Use of virus, viral particle or viral elements as a vector
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14051—Methods of production or purification of viral material
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
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    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
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    • C12N2800/00—Nucleic acids vectors
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  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
KR1020237042063A 2021-05-18 2022-05-17 바이러스 벡터 생산 시스템, 바이러스 벡터 생산을 위한 조작된 세포 및 이의 사용 방법 Pending KR20240019120A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163189771P 2021-05-18 2021-05-18
US63/189,771 2021-05-18
PCT/US2022/029601 WO2022245803A2 (en) 2021-05-18 2022-05-17 Viral vector production systems, engineered cells for viral vector production, and methods of use thereof

Publications (1)

Publication Number Publication Date
KR20240019120A true KR20240019120A (ko) 2024-02-14

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KR1020237042063A Pending KR20240019120A (ko) 2021-05-18 2022-05-17 바이러스 벡터 생산 시스템, 바이러스 벡터 생산을 위한 조작된 세포 및 이의 사용 방법

Country Status (11)

Country Link
US (1) US20240254513A1 (https=)
EP (1) EP4352220A4 (https=)
JP (1) JP2024519090A (https=)
KR (1) KR20240019120A (https=)
CN (1) CN117651775A (https=)
AU (1) AU2022277418A1 (https=)
BR (1) BR112023024205A2 (https=)
CA (1) CA3220476A1 (https=)
IL (1) IL308569A (https=)
MX (1) MX2023013795A (https=)
WO (1) WO2022245803A2 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119662733B (zh) * 2024-12-09 2025-10-28 广州派真生物技术有限公司 一种高产rAAV的质粒系统及应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9388425B2 (en) * 2006-10-20 2016-07-12 Trustees Of Boston University Tunable genetic switch for regulating gene expression
WO2008134593A1 (en) * 2007-04-25 2008-11-06 President And Fellows Of Harvard College Molecular circuits
EP3288594B1 (en) * 2015-04-27 2022-06-29 The Trustees of The University of Pennsylvania Dual aav vector system for crispr/cas9 mediated correction of human disease
US11767539B2 (en) * 2017-05-12 2023-09-26 University Of Massachusetts Viral vector production
US11970720B2 (en) * 2017-08-22 2024-04-30 Salk Institute For Biological Studies RNA targeting methods and compositions

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AU2022277418A1 (en) 2023-11-30
EP4352220A2 (en) 2024-04-17
IL308569A (en) 2024-01-01
US20240254513A1 (en) 2024-08-01
BR112023024205A2 (pt) 2024-01-30
CA3220476A1 (en) 2022-11-24
WO2022245803A3 (en) 2022-12-29
JP2024519090A (ja) 2024-05-08
WO2022245803A2 (en) 2022-11-24
EP4352220A4 (en) 2025-09-24
MX2023013795A (es) 2024-02-15
CN117651775A (zh) 2024-03-05

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