KR20240003972A - Method for the production of extract with increased resveratrol derivatives contents and the cosmetic composition - Google Patents

Abstract

The present invention relates to a method for producing a fermented extract of lactic acid bacteria from barberry with an increased content of resveratrol derivatives and a cosmetic composition containing the same, which provides excellent effects in preventing skin aging and improving wrinkles.

Description

Method for producing an extract with increased resveratrol derivatives contents and a cosmetic composition containing the same {Method for the production of extract with increased resveratrol derivatives contents and the cosmetic composition}

The present invention relates to a method for producing a fermented lactic acid bacteria extract of barberry lactic acid and a cosmetic composition containing the same. More specifically, it relates to a method for producing a fermented lactic acid bacteria extract from barberry lactic acid with increased resveratrol derivative content and a cosmetic composition containing the same.

Resveratrol is a stilbene-based substance and is a phytochemical mainly found in vegetables and fruits (pine trees, peanuts, grapevines, etc.). It is effective in preventing cardiovascular disease and metabolic syndrome due to its strong antioxidant properties, and various effects for improving human health have been confirmed, such as cognitive dysfunction, inhibition of cancer cell growth, and cancer prevention efficacy. In addition, resveratrol is a phytoalexin produced to protect itself from physical, chemical, and biological stresses in the external environment such as mold, bacteria, and UV irradiation, so it protects the plant resources themselves from external stimuli such as UV. Various studies have been attempted to improve antibacterial properties and increase the resveratrol content through treatment. However, resveratrol is unstable in environments exposed to light and oxygen or with strong pH conditions, which limits its use.

Meanwhile, viniferin is one of the derivatives or dimers of resveratrol. Similar to resveratrol, it has various physiological activities such as anti-cancer, anti-viral, anti-inflammatory, anti-aging, and antioxidant, and is active with strong antioxidant activity. It is known to prevent oxygen from changing into melanin-forming cells, stop the circulatory reaction of oxidative stress, and prevent cell inflammation in the skin.

Meanwhile, Scirpus fluviatilis is a perennial herb that grows well at the edges of ponds and swamps. It belongs to the Cyperaceae family and is distributed in Korea, Japan, China, and Taiwan. The height is 80 ~ 150 cm, the stem is triangular, and comes from a thick underground stem. The leaves hang on the lower part of the stem, are longer and flatter than the stem, are 5 ~ 11 mm wide, and the leaf sheath is sometimes brown. Flowers bloom from July to October, and branches with 1 to 4 spikelets line up in a corymb at the end of the stem. The fruit is an achene that ripens in October, is triangular, long oval shaped, and is grayish brown. As the underground stem extends, tubers with a diameter of 3 to 4 cm are formed. They are collected in the fall, dried, and used as medicine. The rhizome is called Gyeongsamneung (京三稜), and in addition, there are Samneung, Gwangsamneung, and Heuksamneung. It mainly relieves stagnant blood and is widely used for bruises, postpartum abdominal pain, and menstrual pain. In addition, boiling it in water has the effect of improving blood flow and improving digestion. Barberry tubers are known to contain astringenin, betulin, betulinaldehyde, betulic acid, lupeol, resveraterol, and scirpusin A and B.

Republic of Korea Patent Publication No. 10-2020-0004134 (January 13, 2020) discloses a skin whitening, anti-aging, wrinkle improvement, regeneration, elasticity, moisturizing, UV protection, skin damage relief, and atopic dermatitis containing grape callus culture fluid as an active ingredient. A composition for improvement is disclosed. Republic of Korea Patent Publication No. 10-2020-0011238 (2020.02.03.) discloses a method for mass producing viniferine and terostilbene from blueberry callus. Republic of Korea Patent Publication No. 10-2020-0011239 (2020.02.03.) discloses a method for producing a callus extract containing increased ε-viniferin content from blueberries.

As a result of researching the applicability of various hitherto unknown natural products as cosmetics, the present inventors confirmed that when barberry was fermented with lactic acid bacteria, resveratrol was converted to produce ε-viniferin, and based on this, it was found that it was used as an anti-aging agent for skin aging or wrinkle improvement. After discovering that it could be developed into a cosmetic composition, the present invention was completed.

Accordingly, the present invention seeks to develop and provide a method for producing a fermented extract of Lactobacillus barberry with increased resveratrol derivative content and a cosmetic composition containing the same for preventing skin aging and improving wrinkles.

The present invention provides a cosmetic composition for improving skin aging, which is obtained by fermenting barberry extract with lactic acid bacteria, and contains 'barberry lactic acid bacteria fermentation extract' containing ε-viniferin, a resveratrol derivative.

In addition, the present invention provides a cosmetic composition for improving skin wrinkles, which is obtained by fermenting barberry extract with lactic acid bacteria, and contains 'barberry lactic acid bacteria fermentation extract' containing ε-viniferin, a resveratrol derivative.

In the cosmetic composition of the present invention, the barberry extract is preferably an extract obtained by extracting one or more selected from the leaves, fruits, stems, roots, and flowers of barberry.

In the cosmetic composition of the present invention, the lactic acid bacteria are preferably microorganisms of the genus Lactobascillus ( Lactobascillus sp. ) or Leuconostoc sp .

In the cosmetic composition of the present invention, the 'barberry lactic acid bacteria fermentation extract' is preferably contained in an amount of 0.001 to 30% by weight based on the total weight of the cosmetic composition.

The present invention provides a fermented extract of lactic acid bacteria from barberry containing ε-viniferin, a resveratrol derivative. In addition, the present invention provides a cosmetic composition for preventing skin aging and improving wrinkles containing the fermented extract of lactic acid bacteria from barberry with an increased content of the resveratrol derivative of the present invention.

Figure 1 shows a comparison of the contents of resveratrol and ε-viniferin confirmed in Examples 1 and 2.
Figure 2 shows the results of comparing the antioxidant effects confirmed in Examples 1 and 2.
Figure 3 shows the results comparing the effects of Examples 1 and 2 on inhibiting MMP-1 production.

The present invention provides a method for producing a fermented lactic acid bacteria extract containing ε-viniferin, a resveratrol derivative, and a cosmetic composition containing the same for improving skin aging and wrinkles. According to the following experiment, it was confirmed that the fermented barberry lactic acid bacteria extract according to the present invention exhibits excellent antioxidant and MMP-1 inhibitory effects.

The present inventors attempted to discover a natural material that has anti-aging and wrinkle-improving effects and is free of toxicity and side effects by converting resveratrol from barberry into ε-viniferin, a resveratrol derivative, through lactic acid fermentation.

The barberry extract used in the present invention can preferably be extracted from one or more of the leaves, stems, flowers, fruits, and roots of barberry, but is most preferably extracted from the roots.

The method of obtaining the 'Barberry extract' used for lactic acid bacteria fermentation in the present invention is not limited to a special one, and various extraction methods commonly used in the technical field to which the present invention pertains can be used. For example, purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, and hexane can be extracted using each solvent alone or a mixture of two or more of these solvents as the extraction solvent. You can. Preferably, it is extracted with a 70% (v/v) ethanol aqueous solution at room temperature for 3 days.

In addition, the barberry extract used in the present invention includes not only extracts using the above-described extraction solvent but also extracts extracted by other methods. For example, extraction by ultrasonic extraction; Extraction by ultra-high pressure extraction method; Separation by floating density difference using an ultracentrifuge; Separation using an ultrafiltration membrane with a constant molecular weight cut-off value; It also includes extracts that have been extracted or separated/purified by additional methods, such as separation by various chromatographs, for example, chromatographs designed for separation based on size, charge, hydrophobicity, or affinity.

After forming a medium with the barberry extract extracted by the above method, inoculating and culturing lactic acid bacteria, the fermented barberry lactic acid bacteria extract of the present invention can be prepared. According to a preferred embodiment of the present invention, the lactic acid bacteria culture medium of the present invention may additionally contain a carbon source, a nitrogen source, trace elements, and a surfactant in addition to the extract.

In the medium of the present invention, at least one selected from the group consisting of dextrose, glucose, sucrose, maltose, fructose, lactose, xylose, galactose and arabinose can be used, and is preferred. Alternatively, dextrose may be used. The amount of carbon source used is preferably 1 to 20% by weight, more preferably 2 to 10% by weight, based on the total weight.

The nitrogen source that can be used in the medium of the present invention is at least one selected from the group consisting of yeast extract, soytone, peptone, beef extract, tryptone, and casitone, Preferably, a nitrogen source containing a mixture of yeast extract, beef extract, and peptone can be used. The amount of nitrogen source used is preferably 1 to 20% by weight, more preferably 1 to 10% by weight, based on the total weight.

In addition to the carbon source and nitrogen source, minerals such as minerals may be used, and at least one selected from the group consisting of dipotassium phosphate, magnesium sulfate, ammonium citrate, sodium acetate, manganic sulfate, and calcium chloride may be used, preferably A mineral mixture of dipotthesium phosphate, magnesium sulfate, ammonium citrate, and sodium acetate can be used. The amount of the trace element used is preferably 0.001 to 5% by weight, more preferably 0.01 to 2% by weight, based on the total weight.

As the lactic acid bacteria used in the present invention, it is better to use microorganisms of the genus Lactobascillus or Leuconostoc sp . Preferably, Lactobascillus sp. , Lactobacillus sakei , Lactobacillus brevis, Lactobacillus acidophillus , Lactobacillus rhamnosus , Lactobacillus At least one strain selected from the group consisting of Lactobacillus Plantarum and Lactobacillus bulgaricus can be used. The inoculation amount of the lactic acid bacteria is preferably such that the initial number of bacteria after inoculation is 10 ⁴ to 10 ⁷ cfu/ml. Fermentation proceeds for 24 to 36 hours under conditions of pH 5 to 7 and temperature of 30 to 40°C while stirring the medium containing barberry extract inoculated with lactic acid bacteria. After fermentation is complete, it is deactivated and distilled under reduced pressure to remove water to produce fermented barberry lactic acid bacteria extract.

The fermented barberry lactic acid bacteria extract prepared in this way can be usefully used in cosmetic compositions for preventing skin aging or improving wrinkles. Specifically, the barberry lactic acid bacteria fermentation extract prepared by removing the bacteria and insoluble components from the barberry lactic acid bacteria culture may be added to the cosmetic composition in an amount commonly used in the technical field to which this technology belongs, but preferably in the total weight. It is better to add 0.001 to 30% by weight, more preferably 0.1 to 10% by weight. If it is included in less than 0.001% by weight, the effectiveness of various skin functionalities identified in the present invention is minimal, and if it is included in more than 30.0% by weight, the efficiency is low compared to the amount of material input, making it uneconomical.

The present invention uses the fermented barberry lactic acid bacteria extract extracted or separated/purified as described above with a solvent selected from purified water, methanol, ethanol, glycerin, ethyl acetate, butylene glycol, propylene glycol, dichloromethane, hexane, and mixtures thereof. After reflux extraction and cold immersion, the filtered filtrate can be transferred to a concentration tank and freeze-dried. In addition, in the present invention, the extract, vacuum concentrate or freeze-dried product obtained in this way can be formulated into a liquid form using one or more solvents selected from purified water, ethanol, butylene glycol, and propylene glycol. It can also be used in this formulated form by adding it to a cosmetic composition.

The cosmetic composition of the present invention includes, for example, lotion, gel, water-soluble liquid, cream, essence, ointment, oil-in-water or water-in-oil makeup base, foundation, skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, It may be one or more selected from the group consisting of cheek color, eyebrow pencil, and scalp formulation.

The term “about” means 30, 25, 20, 15, 10, 9, 8, 7, 6, 5, 9, 8, 7, 6, 5, or means a quantity, level, value, number, frequency, percentage, dimension, size, volume, weight or length that varies by 4, 3, 2 or 1%.

Throughout this specification, unless the context otherwise requires, the words "comprise" and "comprising" include a given step or component, or group of steps or components, but any other step or component, or It should be understood to imply that no step or group of components is excluded.

In addition, the cosmetic composition according to the present invention may preferably contain, in addition to the extract, other ingredients that can provide a synergistic effect on the main effect within a range that does not impair the main effect aimed at by the present invention. For example, it may include conventional auxiliaries such as antioxidants, stabilizers, solubilizers, vitamins, pigments and perfumes, and carriers.

The cosmetic composition of the present invention can be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, ointment, powder, soap, It can be formulated into surfactant-containing cleansing, oil, pack, massage cream and spray, etc., but is not limited thereto. More specifically, water-soluble liquid, flexible lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or oil-in-water or water-in-oil type makeup base, foundation, It can be manufactured in one or more formulations selected from skin cover, lipstick, lip gloss, face powder, two-way cake, eye shadow, cheek color, eyebrow pencil, and scalp.

When the formulation of the cosmetic composition of the present invention is a paste, cream or gel, the carrier ingredient may include animal oil, vegetable oil, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide. This can be used.

When the formulation of the cosmetic composition of the present invention is a solution or emulsion, a solvent, solubilizing agent, or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, and propylene. There are fatty acid esters of glycol, 1,3-butylglycol oil, glycerol aliphatic esters, polyethylene glycol or sorbitan.

When the formulation of the cosmetic composition of the present invention is a suspension, the carrier component includes water, a liquid diluent such as ethanol or propylene glycol, and a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester, and polyoxyethylene sorbitan ester. , microcrystalline cellulose, aluminum metahydroxide, bentonite, agar, or tracant, etc. can be used.

When the formulation of the cosmetic composition of the present invention is powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder can be used as the carrier ingredient. In particular, when the cosmetic composition is a spray, chlorofluorohydride may be used as a carrier ingredient. May contain propellants such as carbon, propane/butane or dimethyl ether.

When the formulation of the cosmetic composition of the present invention is a surfactant-containing cleansing agent, the carrier ingredients include aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, Fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester can be used.

When the cosmetic composition of the present invention is a soap, a surfactant-containing cleansing formulation, or a surfactant-free cleansing formulation, it can be applied to the skin and then wiped off, removed, or washed with water. As a specific example, the soap is liquid soap, powdered soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is cleansing foam, cleansing water, cleansing towel, and cleansing pack, and the surfactant-free cleansing formulation is cleansing cream. , cleansing lotion, cleansing water, and cleansing gel, but are not limited thereto.

All technical terms used in the present invention, unless otherwise defined, have the following definitions and correspond to the meaning as generally understood by a person skilled in the art in the relevant field of the present invention. In addition, although preferred methods and samples are described in this specification, similar or equivalent methods are also included in the scope of the present invention.

Hereinafter, the contents of the present invention will be described in detail through the following examples and experimental examples. However, the scope of the present invention is not limited to the following examples and experimental examples, and includes modifications of the technical idea equivalent thereto.

[Example 1: Preparation of barberry extract]

After drying the roots of barberry in the shade and crushing them, 5 times the amount of 70% (v/v) ethanol aqueous solution was added to 100 g of the crushed material, and extraction was performed at room temperature for 3 days. The extract obtained by extraction was filtered through Whatman #5 filter paper. The filtered extract was concentrated under reduced pressure and freeze-dried below 50°C.

A barberry extract was prepared by dispersing and dissolving the concentrated under reduced pressure and freeze-dried product using purified water to contain 10.0%.

[Examples 2 to 7: Preparation of barberry lactic acid bacteria fermentation extract]

Fermentation of the barberry extract prepared in Example 1 was carried out as follows.

First, 1 L of purified water, 20.0 g of dextrose, 10.0 g of beef extract, 10.0 g of peptone, 5.0 g of yeast extract, 5.0 g of sodium acetate, 2.0 g of ammonium citrate, 0.1 g of magnesium sulfate, 0.05 g of manganic sulfate, 2.0 g of dipotassium phosphate. MRS medium was prepared by mixing and then sterilized at 121°C for 15 minutes. After cooling the medium to 35°C, mycelium of the Lactobacillus genus was inoculated at 1×10 ⁵ cfu/ml each and cultured for about 3 days at 37°C and pH 5 conditions.

In the barberry extract, the strains Lactobascillus sp. , Lactobacillus sakei , Lactobacillus brevis , Lactobacillus acidophillus and Leuconostoc were cultured under the above conditions. ( Leuconostoc sp. ) Inoculate 10 ml each of Leuconostoc mesenteroides , Leuconostoc citreum, and Leuconostoc lactis and incubate approximately 10 ml at 37°C and pH 5. Cultured for 3 days.

After fermentation was completed, 2 ml of 1mM hydrogen peroxide was added, reacted for 5 minutes, and then deactivated. After Whatman filter, concentrated under reduced pressure and freeze-dried, a fermented extract of Lactic Acid bacteria was prepared.

Lactic acid bacteria used in Examples 2 to 7 classification strain Example 2 Lactobacillus sakei (KCTC3603) Example 3 Lactobacillus brevis (KCTC3498) Example 4 Lactobacillus acidophillus (KCTC3145) Example 5 Leuconostoc mesenteroides (KCTC3722) Example 6 Leuconostoc citreum (KCTC.3526) Example 7 Leuconostoc lactis (KCTC3528)

[Experimental Example 1: Change in resveratrol derivative content by fermented barberry lactic acid bacteria extract]

Changes in the content of resveratrol and the resveratrol derivative ε-viniferin were confirmed in the barberry extract of Example 1 and the barberry lactic acid bacteria fermentation extract obtained in Examples 2 to 7.

Each extract was dissolved in methanol, passed through a 0.45 μm PVDF syringe filter (Whatman, Maidstone, England), and then used for high-performance liquid chromatography (HPLC, Alliance HPLC System e2695/2998, USA). The column was Optima Pak. C 18 (250 mm * 4.6 um), detector is UV (280 nm), sample injection amount is 10 ul, temperature is 35℃, mobile phase is acetonitrile for HPLC (A): water (B) = 30:70. was confirmed.

Resveratrol and ε-viniferin contents of Examples 1 to 7 sample Resveratrol (㎍/g) ε-viniferin (㎍/g) Example 1 45 N.D. Example 2 8.1 18.45 Example 3 40.5 2.25 Example 4 41.4 1.8 Example 5 42.705 1.1475 Example 6 43.515 0.7425 Example 7 44.46 0.27

As a result of the experiment, as shown in Figure 1 and Table 2, it was confirmed that the ε-viniferin content of Example 2 was the highest at 18.45 ㎍/g, and it was confirmed that the resveratrol of Example 1 was converted to ε-viniferine through fermentation. did.

[Experimental Example 2: Antioxidant effect of barberry lactic acid bacteria fermentation extract using DPPH method]

In this experimental example, the antioxidant effect of Examples 1 and 2 was attempted to be confirmed.

Among antioxidant measurement methods, the representative DPPH (2,2-diphenyl-1-picrylhydrazyl) radical has a maximum absorbance at 560 nm when dissolved in an organic solvent. At this time, the antioxidant eliminates the DPPH radical and loses its original color and turns yellow. I order it. The reagent used is 2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl free radical (Aldrich Chem. Co., MW=618.76). 61.88 mg of 0.1 mM solution is dissolved in methanol to make 100 ml. 0.15 ml of sample solution was added to 0.15 ml of 0.1 mM DPPH solution in a 96-well plate, stirred quickly, and incubated at 25°C for 10 minutes, and then the absorbance St at 560 nm was measured. The blank of the sample solution was used to measure the absorbance So in the same manner as when measuring the absorbance St, except that methanol was used instead of the 0.1 mM DPPH solution. In the blank test, the absorbance Bt was measured in the same manner as when measuring the absorbance St, except that distilled water was used instead of the sample solution. The blank of the blank test was operated in the same manner as when measuring the absorbance St, except that methanol was used instead of the 0.1 mM DPPH solution and distilled water was used instead of the sample solution, and the absorbance Bo was measured.

The results of the free radical scavenging rate (%) were calculated according to Equation 1, and the results are shown in Figure 2.

[Equation 1]

St: Absorbance at 560 nm after free radical scavenging of the sample

Bt: Absorbance at 560 nm after free radical scavenging of blank test solution

So: Absorbance at 560 nm before reaction in the absence of free radicals in the sample

Bo: Absorbance at 560 nm before reaction in the blank test solution without addition of free radicals

As shown in Figure 2, it can be seen that the antioxidant effect increases in a concentration-dependent manner in Example 2. In addition, it was found that at a concentration of 100 μg/ml, the effect was almost similar to the effect of 100 μM L-ascorbic acid in the positive control group.

Therefore, it was confirmed that the composition containing the fermented lactic acid bacteria extract of barberry of the present invention has an excellent skin antioxidant effect and has an anti-skin aging effect.

[Experimental Example 2: Measurement of MMP-1 production inhibition ability of barberry lactic acid bacteria fermentation extract]

In this experimental example, the effect of Examples 1 and 2 on suppressing MMP-1 production was sought to be confirmed.

Human dermal fibroblasts (HDFn) were distributed in a 24 well-plate at a concentration of 5×10 ⁴ cells/well and stabilized in an incubator at 37°C and 5% CO ₂ . After rinsing twice with PBS, UVA was irradiated at 1 J/cm ² in the presence of PBS. Simultaneously with changing the medium to which 2% FBS was added, Examples 1, 2, and 5 were diluted and treated according to concentration, and 400 μM adenosine (an anti-wrinkle functional notice raw material) was used as a positive control. After 48 hours, the supernatant was collected, and the remaining cells were treated with MTT solution to confirm cytotoxicity. The R&D systems kit (DY901) was used to check the expression level of MMP-1 using the collected supernatant. After MMP-1 ELISA assay, the absorbance was measured at a wavelength of 450 nm to calculate the amount of MMP-1 produced, and then the inhibition rate (%) of MMP-1 expression was calculated compared to the control group. The results are shown in Figure 3.

[Equation 2]

Looking at Figure 3, it was confirmed that Example 2 of the present invention had excellent efficacy in inhibiting MMP-1 production. In addition, Example 2 was confirmed to exhibit similar efficacy (approximately 55.5% inhibition of MMP-1 production) to 100 μg/ml of adenosine, a substance known to have the effect of inhibiting MMP-1 production. From the above results, it was confirmed that the fermented barberry lactic acid bacteria extract of the present invention exhibits an excellent skin wrinkle improvement effect.

[Formulation Example 1: Preparation of cosmetics containing barberry lactic acid bacteria fermentation extract]

A cosmetic composition containing 5.0% by weight of the fermented lactic acid bacteria extract of Example 2 and a comparative formulation were prepared with the compositions shown in Table 3 below.

Cosmetic composition containing fermented barberry lactic acid bacteria extract (% by weight) division ingredient Formulation Example 1 Comparative formulation example 1 A Example 2 5.0 - EDTA-2Na 0.02 0.02 Purified water up to 100 up to 100 B Cetostearyl alcohol 2.0 2.0 Glyceryl Stearate 1.5 1.5 microcrystalline 0.7 0.7 squalane 5.0 5.0 liquid paraffin 3.0 3.0 Trioctanoin 5.0 5.0 polysorbate 1.2 1.2 Sorbitan Stearate 0.5 0.5 Tocopheryl Acetate 0.2 0.2 Cyclomethicone 3.0 3.0 BHT 0.05 0.05 C Appropriate amount Appropriate amount Appropriate amount

[Experimental Example 3: Skin wrinkle improvement effect of fermented barberry lactic acid bacteria extract]

In this experimental example, a human application test was conducted on the skin wrinkle improvement effect of cosmetics containing fermented extract of lactic acid bacteria from barberry. The test was evaluated through actual use tests of each cosmetic.

The human application test confirmed and compared the skin wrinkle improvement effect caused by the use of cosmetics containing the cosmetic composition of Formulation Example 1, which contains the fermented lactic acid bacteria extract of barberry, and Comparative Formulation Example 1, which does not contain the fermented lactic acid bacteria extract of barberry.

Experimenter (female aged 20 - 35 years old) Cosmetic composition containing fermented extract of lactic acid bacteria of barberry on 20 women Example 19 Comparative Example 1, which is an experimental group and a control group, was applied to the right side of the face and Example 19 to the left side of the face, respectively. The experiment was conducted by applying the product. The effect of improving skin wrinkles was evaluated for each subject before and after using the product for 8 weeks through visual observation by an experienced doctor and instrumental measurement (cutometer SEM 575, C+K Electronic Co., Germany), and the experimental results were It is shown in Table 4 below.

Wrinkle improvement effect of a cosmetic composition containing fermented extract of lactic acid bacteria from Barberry (visual evaluation and device evaluation) Wrinkle improvement effect Improvement rate (%) Great a little doesn't exist Formulation Example 1 14 2 4 75.0 Comparative formulation example 1 0 11 9 27.5

As can be seen from the results in Table 4, the cosmetic composition of Formulation Example 1 containing the fermented lactic acid bacteria extract of barberry of the present invention showed a high improvement rate compared to Comparative Formulation Example 1, which was the control group.

Claims (5)

A cosmetic composition for improving skin aging containing 'Barberry lactic acid bacteria fermentation extract', which is obtained by fermenting barberry extract with lactic acid bacteria and contains ε-viniferin, a resveratrol derivative.
A cosmetic composition for improving skin wrinkles, which is obtained by fermenting barberry extract with lactic acid bacteria, and contains 'barberry lactic acid bacteria fermentation extract' containing ε-viniferin, a resveratrol derivative.
According to claim 1 or 2,
The extract of barberry,
A cosmetic composition characterized in that it is an extract obtained by extracting one or more selected from the leaves, fruits, stems, roots, and flowers of barberry.
According to claim 1 or 2,
The lactic acid bacteria,
A cosmetic composition characterized in that it is a microorganism of the genus Lactobascillus ( Lactobascillus sp. ) or Leuconostoc sp .
According to claim 1 or 2,
The ‘barberry lactic acid bacteria fermentation extract’,
A cosmetic composition characterized in that it contains 0.001 to 30% by weight based on the total weight of the cosmetic composition.