KR20230157387A - Cas 효소를 이용한 다중 편집 - Google Patents

Cas 효소를 이용한 다중 편집 Download PDF

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Publication number
KR20230157387A
KR20230157387A KR1020237034371A KR20237034371A KR20230157387A KR 20230157387 A KR20230157387 A KR 20230157387A KR 1020237034371 A KR1020237034371 A KR 1020237034371A KR 20237034371 A KR20237034371 A KR 20237034371A KR 20230157387 A KR20230157387 A KR 20230157387A
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South Korea
Prior art keywords
sequence
rna
target
endonuclease
locus
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Pending
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English (en)
Korean (ko)
Inventor
브라이언 토마스
크리스토퍼 브라운
아우드라 데보토
크리스티나 버터필드
리사 알렉산더
다니엘라 에스 에이 골츠만
그레그 코스트
레베카 라모스
디에고 에스피노사
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메타지노미, 인크.
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Publication of KR20230157387A publication Critical patent/KR20230157387A/ko
Pending legal-status Critical Current

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
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    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
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    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1138—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against receptors or cell surface proteins
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
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KR1020237034371A 2021-03-19 2022-03-18 Cas 효소를 이용한 다중 편집 Pending KR20230157387A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202163163510P 2021-03-19 2021-03-19
US63/163,510 2021-03-19
US202163186506P 2021-05-10 2021-05-10
US63/186,506 2021-05-10
US202163241916P 2021-09-08 2021-09-08
US63/241,916 2021-09-08
PCT/US2022/021004 WO2022198080A1 (en) 2021-03-19 2022-03-18 Multiplex editing with cas enzymes

Publications (1)

Publication Number Publication Date
KR20230157387A true KR20230157387A (ko) 2023-11-16

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KR1020237034371A Pending KR20230157387A (ko) 2021-03-19 2022-03-18 Cas 효소를 이용한 다중 편집

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Country Link
US (1) US20250075233A1 (enExample)
EP (1) EP4308699A4 (enExample)
JP (1) JP7877351B2 (enExample)
KR (1) KR20230157387A (enExample)
AU (1) AU2022237663A1 (enExample)
BR (1) BR112023018948A2 (enExample)
CA (1) CA3210361A1 (enExample)
MX (1) MX2023010969A (enExample)
WO (1) WO2022198080A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021178933A2 (en) 2020-03-06 2021-09-10 Metagenomi Ip Technologies, Llc Class ii, type v crispr systems
US10913941B2 (en) 2019-02-14 2021-02-09 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
CN116096892A (zh) * 2020-05-08 2023-05-09 宏基因组学公司 具有RuvC结构域的酶
KR20230134543A (ko) * 2021-01-22 2023-09-21 메타지노미, 인크. 신규한 조작된 뉴클레아제 및 키메라 뉴클레아제
CN119452085A (zh) 2021-08-27 2025-02-14 宏基因组学公司 具有ruvc结构域的酶
KR20240110603A (ko) 2021-11-24 2024-07-15 메타지노미, 인크. 엔도뉴클레아제 시스템
WO2024127370A1 (en) * 2022-12-16 2024-06-20 LifeEDIT Therapeutics, Inc. Guide rnas that target trac gene and methods of use

Family Cites Families (14)

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Publication number Priority date Publication date Assignee Title
AU2003233634A1 (en) 2002-05-20 2003-12-12 Pharmacia Corporation Antisense modulation of glucocorticoid receptor expression
US20150017136A1 (en) 2013-07-15 2015-01-15 Cellectis Methods for engineering allogeneic and highly active t cell for immunotherapy
JP6608807B2 (ja) * 2013-05-29 2019-11-20 セレクティス Ｒｎａガイドｃａｓヌクレアーゼ系を用いることによって免疫療法のためにｔ細胞を操作するための方法
AU2016262093B2 (en) 2015-05-13 2022-08-18 Seattle Children' S Hospital (Dba Seattle Children 's Research Institute) Enhancing endonuclease based gene editing in primary cells
MX2018010484A (es) 2016-03-04 2019-01-10 Editas Medicine Inc Métodos, composiciones y componentes relacionados con crispr-cpf1 para la inmunoterapia contra el cáncer.
BR112019023608A2 (pt) 2017-05-12 2020-05-26 Crispr Therapeutics Ag Materiais e métodos para células manipuladas e seus usos em imuno-oncologia
EP3686275A4 (en) 2017-09-18 2021-09-29 Edigene Inc. GENE EDITING LYMPHOCYTE T AND RELATED USE
US12359223B2 (en) * 2018-01-30 2025-07-15 Editas Medicine, Inc. Systems and methods for modulating chromosomal rearrangements
US12275951B2 (en) 2018-02-06 2025-04-15 Ohio State Innovation Foundation Engineered guide RNA and uses thereof
BR112020015488A2 (pt) 2018-02-08 2020-12-08 Sangamo Therapeutics, Inc. Nucleases alvo-específicas modificadas
KR20240007322A (ko) * 2019-02-14 2024-01-16 메타지노미, 인크. Ruvc 도메인이 존재하는 효소
US10913941B2 (en) * 2019-02-14 2021-02-09 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
EP3938510A1 (en) 2019-03-11 2022-01-19 Sorrento Therapeutics, Inc. Improved process for integration of dna constructs using rna-guided endonucleases
GB201907733D0 (en) * 2019-05-31 2019-07-17 Governing Council Of The Univ Of Toronto Methods and compositions for multiplex gene editing

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CA3210361A1 (en) 2022-09-22
US20250075233A1 (en) 2025-03-06
EP4308699A4 (en) 2025-12-31
AU2022237663A9 (en) 2023-10-26
MX2023010969A (es) 2023-09-27
AU2022237663A1 (en) 2023-10-12
BR112023018948A2 (pt) 2023-10-17
JP7877351B2 (ja) 2026-06-22
JP2024515936A (ja) 2024-04-11
WO2022198080A1 (en) 2022-09-22
EP4308699A1 (en) 2024-01-24

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