KR20230155121A - Novel burkholderia contaminans strain and composition for controlling plant diseases using the strain or culture filtrate thereof - Google Patents

Abstract

The present invention relates to the Burkholderia contaminans AY001 strain (accession number: KACC92414P), and to a composition for controlling plant diseases using the AY001 strain (accession number: KACC92414P) and its culture filtrate. The strain or culture filtrate according to the present invention improves the stress resistance of plants, thereby enabling sustainable agriculture and eliminating or minimizing the use of chemical pesticides.

Description

New Burkholderia contaminans strains and compositions for controlling plant diseases using strains or their culture filtrates {NOVEL BURKHOLDERIA CONTAMINANS STRAIN AND COMPOSITION FOR CONTROLLING PLANT DISEASES USING THE STRAIN OR CULTURE FILTRATE THEREOF}

The present invention relates to a composition for controlling plant diseases using a novel microorganism or its culture filtrate, and more specifically, to a composition for controlling plant diseases using a novel Burkholderia contaminans strain or its culture filtrate.

In modern agriculture, there is a need to develop a biological control agent that can replace or supplement chemical pesticides due to problems such as environmental pollution and pesticide residues caused by the continuous use of chemical pesticides. The development of biological control agents using various microorganisms existing in nature is essential for sustainable agriculture by reducing the use of chemical pesticides.

US registered patent US-10092622 (2018.10.09) discloses the antifungal effect against Fusarium oxysporum using Occidiofungin, one of the extracts of Burkholderia contaminans. I'm doing it. In other words, it discloses a limited use in disclosing the control effect against specific fungi.

Tomato is a major vegetable crop, and there are cases where it is not easy to suppress pathogens through chemical control. Taking tomatoes as an example, there are about 27 known diseases that occur in domestic tomatoes, and phytopathogenic bacteria and fungi are involved in them. Diseases that mainly occur in tomatoes and cause problems during cultivation include gray mold, leaf mold, powdery mildew, leaf blight, and green blight. These diseases are a major cause of reduced yield when left untreated in tomato cultivation, but can be controlled by applying appropriate chemicals. Tomato wilt disease (or Tomato Fusarium wilt disease) is an important soil-borne disease that occurs widely in tomato growing areas across the country and causes a lot of damage. Tomato wilt pathogens overwinter as mycelia or thick spores in the tissues or soil of diseased plants and become a primary source of infection again the following year. The distance traveled by water is very short, and it is mainly buried in soil particles and transported long distances through agricultural equipment or people. Pathogens mainly enter through fine roots or wounds, and infections often occur immediately after planting in the field. Tomato wilt disease is less common in cool regions and has the characteristic of not showing symptoms even when infected, but showing symptoms when the temperature rises in the middle or late growth period. Specifically, the temperature at which tomato wilt disease appears is about 24-30℃, and it rarely occurs at temperatures below about 16℃ or above about 35℃. In addition, it generally occurs in acidic soil (pH 4.5-5.5) and sandy loam soil, because the plant becomes stressed and weakened due to an imbalance in soil moisture, making it easy for pathogens to invade. The pathogen is widely distributed in the soil, and the overwintering thick membrane spores survive in the soil for several years without a host, making it a very difficult disease to control.

The purpose of the present invention is to provide a composition for controlling plant diseases using the new Burkholderia contaminans AY001 strain (accession number: KACC92414P) or its culture filtrate, which can be prescribed at the pre-growth stage.

In addition, the purpose of the present invention is to provide a method of eliminating or minimizing the use of chemical pesticides by improving the stress resistance of plants and thereby enabling sustainable agriculture.

The object of the present invention is not limited to what has been mentioned above, and other objects not mentioned will be clearly understood by those skilled in the art from the description below.

In order to solve the above technical problem, according to one aspect of the present invention, Burkholderia contaminans AY001 strain (Accession number: KACC92414P) is provided.

Burkholderia contaminans strain AY001 (accession number: KACC92414P).

Here, it may be provided in a composition for promoting tomato plant growth containing the strain or the culture filtrate of the strain.

Here, a composition for inducing systemic resistance to tomato plant pathogens containing the strain or a culture filtrate of the strain may be provided.

Here, the plant pathogen may be tomato bacterial black spot disease.

Here, a composition for controlling plant diseases for controlling peach fruit rot, pepper anthracnose, pepper late blight, cabbage sclerotia, or tomato wilt disease may be provided, containing the strain or the culture filtrate of the strain as an active ingredient.

Here, peach fruit rot is caused by Fusarium avenaceum, pepper anthracnose is caused by Colletotrichum acutatum , and pepper blight is caused by Phytophthora capsici . Cabbage sclerotia may be caused by Sclerotinia sclerotiorum , and tomato wilt may be caused by Fusarium oxysporum .

Here, a method of inducing systemic resistance to a tomato plant pathogen is provided, comprising treating the strain or the culture filtrate of the strain to a tomato plant, an area around the tomato plant, or both.

Here, the method for enhancing systemic induced resistance may be treating one or more selected from the group consisting of seeds, roots, stems, leaves, and the entire plant of the tomato plant.

Here, a method for promoting tomato plant growth is provided, comprising treating the strain or the culture filtrate of the strain to a tomato plant, an area around the tomato plant, or both.

According to the present invention, a composition for controlling tomato wilt disease using the new Burkholderia contaminans AY001 strain (accession number: KACC92414P), which can be prescribed at the pre-growth stage, and its culture filtrate can be provided.

According to another embodiment of the present invention, the stress resistance of plants is improved, thereby enabling sustainable agriculture and eliminating or minimizing the use of chemical pesticides.

The effects of the present invention are not limited to those mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description below.

Figure 1 shows the activity of Burkholderia contaminans AY001 strain to inhibit the growth of plant pathogenic pepper anthracnose ( Colletotrichum acutatum ), leaf spot fungus ( Alternaria alternata ), and tomato wilt fungus ( Fusarium oxysporum ) on medium. This is the drawing shown.
Figure 2 is a diagram showing that the strain Burkholderia contaminans AY001 belongs to Burkholderia contaminans molecularly through ITS (internal transcribed spacer) sequencing.
Figure 3 shows the AY001 strain suppressing tomato wilt disease caused by Fusarium oxysporum in tomato plants.

The purpose and effects of the present invention, and technical configurations for achieving them, will become clear by referring to the embodiments described in detail below along with the accompanying drawings. In describing the present invention, if it is determined that a detailed description of a known function or configuration may unnecessarily obscure the gist of the present invention, the detailed description will be omitted. And the terms described below are terms defined in consideration of donation in the present invention, and may vary depending on the intention or custom of the user or operator.

However, the present invention is not limited to the embodiments disclosed below and may be implemented in various different forms. These embodiments are merely provided to ensure that the disclosure of the present invention is complete and to fully inform those skilled in the art of the present invention of the scope of the invention, and that the present invention is defined by the scope of the claims. It just becomes. Therefore, the definition should be made based on the contents throughout this specification.

Hereinafter, embodiments of the present invention will be described in detail.

As used herein, “plant” refers to any living organism belonging to the plant kingdom (i.e., any genus/species within the plant kingdom), such as, but not limited to, trees, herbs, shrubs, grasses, vines, ferns, mosses, and green algae. does not Therefore, representative plants to which the composition can be applied include brassicas, bulb vegetables, grains, citrus, cotton, cucurbits, fruit vegetables, leafy vegetables, legumes, oil seed crops, peanuts, pomegranates, root vegetables, tuber vegetables, and corm vegetables. , stone fruits, tobacco, strawberries and other berries, and various ornamental plants. The plant herein may be pepper, tomato, or paprika.

In this specification, “culture medium” or “culture filtrate” refers to the result obtained by inoculating a strain into a medium and culturing it for a certain period of time. The culture medium itself is obtained by culturing the strain according to the present invention in a suitable liquid medium, and the culture medium is filtered or centrifuged to isolate the strain. A filtrate (filtrate or centrifuged supernatant) from which Including but not limited to this. In addition, the probiotic or antibacterial composition may contain a culture such as a solid medium culture or a dried product or extract of the culture, but is not limited thereto, and may further include a suitable excipient or carrier.

The composition of the present invention can be prepared in the form of a liquid fertilizer, and can be used in the form of powder by adding an extender, or can be formulated and granulated. However, there is no particular limitation to the formulation.

The composition for inducing systemic induced resistance in plants of the present invention can increase plant resistance to one or more stresses selected from the group consisting of moisture, temperature, salt, pH, nutrients, and pathogens, inhibit disease development, and promote growth.

As used herein, “stress” may be biotic, abiotic, or biotic stress on plants, and specifically, abiotic stress may be dryness, high temperature, low temperature, high salinity, or nutrient deficiency. The term "abiotic stress" is used herein in its usual sense as the negative effect of abiotic factors on living organisms in a particular environment, and therefore in relation to plants, the negative effects of abiotic factors on plants in a particular environment. It means influence. Biotic stresses include living disturbances such as fungi or harmful insects, while abiotic stressors can be naturally occurring or man-made and include temperature, dry soil, osmotic stress, drought, salt or nutrient deficiencies. These include, all of which can cause harm to plants in the affected area. For example, salt stress may include increased salt concentrations or drought. Nutrient deficiency may be a lack of soil nutrients such as potassium, phosphate or iron in the soil area surrounding the plant. (Soil) Increased stress resistance to nutrient deficiencies, such as phosphate, can be provided by improved solubilization of nutrients lacking in the soil.

The term “resistance (or tolerance) to salt” is used herein in its ordinary sense, which refers to the resistance (tolerance) of plants to salt concentrations. Thus, “increasing the salt tolerance or tolerance of a plant” usually refers to the practice of increasing salt concentrations (in their environment, such as soil or water) when a plant is exposed to salt concentrations higher than what is physiologically acceptable for the plant. It means increasing or improving a plant's ability to endure or endure.

The term “moisture resistance (or tolerance)” or “desiccation resistance (or tolerance)” refers to the ability of a plant to avoid stress and its consequences by maintaining a desirable moisture balance and swelling even when exposed to normally dry conditions. It is also used herein in the meaning of. Therefore, “increasing the water (or desiccation) resistance” of a plant will maintain a more desirable water balance and expansion when exposed to drought conditions where the plant does not receive the required amount of water on a regular basis to maintain water balance and expansion. It means increasing or improving a plant's ability to

The term “plant pathogen” refers to pathogens that invade plants and cause loss or damage to the leaves, stems, roots or fruits of plants, such as fungi, bacteria, viruses and phytoplasma. ) includes.

The term "plant pathogen resistance" or "bacterial resistance" generally refers to the ability to enhance the growth of plants by avoiding infection and growth of pathogens, and enhancing resistance to such pathogens refers to the control, sterilization, and other methods used in the field. Includes the meaning of insect repellent.

The composition for inducing systemic resistance in plants according to the present invention is a plant's resistance to pathogen stress that causes one or more plant diseases selected from the group consisting of Ralstonia solanacearum and Phytophthora capsici, and disease occurrence. It can inhibit and promote growth.

The composition for inducing systemic resistance in plants of the present invention is effective for inhibiting resistance, disease development, and It can promote growth.

The present invention promotes or promotes plant growth by inducing systemic resistance in plants. In the above, 'plant growth' refers to the germination of plants, differentiation of each organ (roots, stems, leaves, fruits, etc.), flowering or development, and increase in size and weight, and increase in yield.

The composition for inducing systemic induced resistance of plants of the present invention includes the strain of the present invention, the strain culture medium, or a mixture thereof, and any other ingredients used in the field to enhance systemic induced resistance or promote growth of plants. may additionally be included. For example, it may further include one or more selected from the group consisting of plant growth regulators, plant growth stimulators, and plant nutrients.

The plant growth regulator, plant growth stimulant or plant nutrient may be a chemical or another strain of bacteria, a herbicide, fungicide, pesticide, fertilizer or any combination thereof.

The composition of the present invention that promotes plant growth may include other beneficial microorganisms, microbial active substances, organic fertilizers, and chemical fertilizers. The other beneficial microorganisms include Bacillus species known to promote plant growth. spp.), Azotobacter spp., Trichoderma spp. and Saccharomyces spp.

The microbial active substances may include enzyme precursors, microbial metabolites, organic acids, carbohydrates, enzymes, and/or trace elements. For example, microbial active substances may include processed enzyme products such as yeast autolysates, humic materials, seaweed extracts, starch, amino acids, and trace elements such as zinc, iron, copper, manganese, bromine, and molybdenum. .

Other beneficial microorganisms, microbial active substances and organic fertilizers may be the same as described above. Chemical fertilizers can contain a variety of chemicals that can provide nitrogen, phosphorus and potassium nutrients to support plant growth. For example, chemical fertilizers may include urea, calcium phosphate, potassium phosphate, and mixed nitrogen-phosphate-potassium (N-P-K) fertilizers. Chemical fertilizers may also include other materials known in the art.

The present invention relates to one or more selected from the group consisting of Burkholderia contaminans AY001 strain (Accession number: KACC92414P), a culture medium of the strain, a composition containing the strain, and a composition containing the culture medium of the strain. It provides a method of inducing systemic resistance in a plant comprising treating the plant, the area surrounding the plant, or both.

The parts of the plant applied to the embodiments of the present invention are not limited. Accordingly, it includes both the whole plant as well as parts of the plant, such as seeds, shoots, stems, roots, leaves, and fruits, as well as the whole plant and parts thereof.

Examples of the present invention can be carried out by immersing or drenching, that is, spraying, the strain, the culture medium culturing the strain, and a composition using the strain into seeds or plants. In the case of the immersion method, the culture solution and preparation can be drenched into the soil around the plant, or the seeds can be immersed in the culture solution and composition and shaken for inoculation.

The surrounding area applied by embodiments of the present invention refers to the range in which the composition of the present invention can exert its effect, and includes both above ground and underground areas. For example, it may be an area within about 2 m, within about 1 m, within about 70 cm, within about 50 cm, within about 25 cm, within about 10 cm, or within about 5 cm around the plant, plant part, or fruit.

Methods according to embodiments of the present invention include applying a composition comprising Burkholderia contaminans AY001 strain at any time during the life cycle of a plant, during one or more stages of the life cycle of the plant, or during the life cycle of the plant. involves application at regular intervals or continuously throughout the life of the plant. Therefore, the composition can be applied as needed. The composition may, for example, be applied to the plant during growth, before and/or during flowering and/or before and/or during seed development. In one example, the composition may be applied before, during and/or immediately after plants are transplanted from one location to another location, such as from a greenhouse or hotbed to the field. In another example, each composition may be applied to the plant multiple times at desired intervals.

The method for inducing systemic resistance of the present invention can improve plant resistance, disease development, and growth against one or more stresses selected from the group consisting of moisture, temperature, salt, pH, nutrients, and pathogens.

The method for inducing systemic resistance in plants according to the present invention can suppress disease development against tomato bacterial disease (Pseudomonas syringae pv. Tomato DC3000: Tomato bacterial black spot disease). Specific details about pathogens are as described above.

The method for enhancing the stress resistance of plants of the present invention is to inhibit the resistance, disease development, or growth of any one or more plants selected from the group consisting of trees, herbs, shrubs, grasses, vines, ferns, moss, green algae, monocots, and dicots. can promote.

The present invention relates to one or more selected from the group consisting of Burkholderia contaminans AY001 strain (Accession number: KACC92414P), a culture medium of the strain, a composition containing the strain, and a composition containing the culture medium of the strain. Provided is a method for promoting plant growth comprising treating the plant, the area around the plant, or both.

The plant growth promotion method of the present invention can promote the growth of any one or more plants selected from the group consisting of trees, herbs, shrubs, grasses, vines, ferns, moss, green algae, monocots, and dicots.

For the present invention, soil located in Andong Agi Mountain (Sugok-ri, Imdong-myeon, Andong-si, Gyeongsangbuk-do) was collected and various bacteria present in the soil were isolated, and among these, plant pathogenic fungi ( Fusarium oxysporum , Fusarium avenaceum , Fusarium solani , Colletotrichum acutatum , Phytophthora capsici , As a result of research to discover a strain exhibiting antibacterial activity against Sclerotinia sclerotiorum, the Burkholderia contaminans AY001 strain of the present invention was newly discovered.

The Burkholderia contaminans AY001 strain of the present invention is capable of producing six types of plant pathogenic fungi ( Fusarium oxysporum (tomato wilt fungus), Fusarium avenaceum (peach fruit rot), Fusarium solani (wilt fungus), and Colletotrichum acutatum (pepper anthracnose) on the medium. ), Phytophthora capsici (red pepper blight), and Sclerotinia sclerotiorum (cabbage sclerotiorum) showed a direct antagonistic effect ( Figure 1 ).

As shown in the dual culture assay results, the mycelial growth of six plant pathogenic fungi was suppressed when cultured simultaneously with the AY001 strain, and mycelial growth of the pathogen was also inhibited in a medium containing the culture filtrate of the AY001 strain. has been shown to be able to suppress.

The Burkholderia contaminans AY001 strain of the present invention was identified through 16S ribosomal RNA (16S rRNA) sequencing and phylogenetic analysis ( Fig. 2 ).

Burkholderia contaminans AY001 strain showed PGP (phosphate and zinc solubilization, protease production, siderophore production, ammonia production, IAA production, nitrogen fixation) activity on the medium ( Figure 3 ).

Fusarium oxysporum f on tomato plants. sp. When inoculated with lycopersici strain KACC 40038 ( FOL ), wilt disease occurred and the plant died, but when inoculated simultaneously with a suspension (2x10 ⁷ cfu/ml or 2x10 ⁵ cfu/ml) of the Burkholderia contaminans AY001 strain of the present invention. The effect of protecting tomato plants from wilt disease caused by Fusarium was shown ( Fig. 4 ). When tomato plants were treated with only a suspension of the Burkholderia contaminans AY001 strain (10 ⁷ cfu/ml or 10 ⁵ cfu/ml), plant growth increased compared to Mock (non-inoculated experimental group), promoting the growth of tomato plants. It was found to be effective ( Figure 5 ).

In order to confirm the induced systemic resistance of the Burkholderia contaminans AY001 strain of the present invention, Pseudomonas syringae pv. tomato DC3000 ( Pst ) and tomato plants were simultaneously inoculated. Pseudomonas syringae pv. When only tomato DC3000 ( Pst ) was inoculated, bacterial black spot appeared on the plant leaves, but when simultaneously inoculated with a suspension ( ^2x107 cfu/ml) of the Burkholderia contaminans AY001 strain of the present invention, the leaves of the tomato plants showed bacterial infection. A protective effect against black spot disease was observed ( Fig. 6 ).

RT-PCR was performed to confirm the expression of genes related to induced systemic resistance of the Burkholderia contaminans AY001 strain of the present invention. PIN2 , LapA , and ACO1 genes were expressed in tomato plants treated with Burkholderia contaminans AY001 strain ( Figure 7 ). This indicates that the AY001 strain has the effect of increasing plant disease resistance in tomato plants.

Gas chromatography mass spectrometry (GC-MS/MS) analysis was performed to analyze substances exhibiting antibacterial activity in the culture filtrate of the Burkholderia contaminans AY001 strain of the present invention ( Fig. 8 ). As a result of the analysis, substances called Pyrrolo[1,2-a]pyrazine-1,4-dione and hexahydro-3- (phenylmethyl) were detected as the main ingredients.

In order to confirm the establishment rate of the Burkholderia contaminans AY001 strain of the present invention on tomato plant roots, it was immersed in AY001 suspension (2x10 ⁷ cfu/ml) and sampled for up to 14 days to show the establishment rate ( Fig. 9 ). This indicates that AY001 causes induced resistance in plants due to its excellent fixation ability in plant roots, making plants less susceptible to disease.

Hereinafter, the present invention will be described in more detail through examples. However, these are only for explaining the present invention in more detail and the scope of the present invention is not limited thereto.

Example 1: Selection and identification of AY001 strain

To classify and identify the AY001 strain, total gDNA was isolated using a gDNA isolation kit. Afterwards, the 16S rRNA portion was amplified through polymerase chain reaction (PCR) using primers 27F (5'-AGTTTGATCCTGGCTCAG-3') and 1492R (5'-GTTACCTTGTTACGACTT-3').

PCR conditions were 95°C once for 5 minutes, repeated 33 times at 94°C for 30 seconds, 58°C for 30 seconds, and 72°C for 40 seconds, and then maintained at 72°C for 10 minutes. After analyzing the base sequence of the amplified PCR part, species with similar base sequences were identified using the BLAST network service at NCBI (National Center for Biotechnology Information).

As a result of phylogenetic analysis, strain AY001 was found to have 100% similarity to Burkholderia contaminans JCK-CSHB12-R in the 16S rRNA base sequence (1393bp), confirming that it is a Burkholderia contaminans species (Figure 2).

Example 2: Evaluation of the inhibitory effect of the AY001 strain on Tomato Fusarium wilt disease

The inhibitory effect of the AY001 strain on tomato Fusarium wilt disease was evaluated. To evaluate the effect of the AY001 strain on suppressing Tomato Fusarium wilt disease, Tomato Fusarium wilt disease spore suspension (1×10 ⁶ conidia/ml) and AY001 suspension (2×10 ⁵ cfu/ml, 2×10 ⁷ cfu) were added to 2-week-old tomato plants. /ml) was treated (Figure 4). In plants treated with a spore suspension ^of the Tomato Fusarium wilt pathogen ( ¹ conidia/ml) and AY001 suspension (2×10 ⁵ cfu/ml, 2×10 ⁷ cfu/ml) simultaneously treated wilting symptoms (Figure 4, FOL + AY001). Looking at the experimental results, in the case of Tomato Fusarium wilt disease, a difference was shown as shown in Figure 4 after 2 weeks of treatment, confirming that there was a disease suppression effect when the AY001 strain was treated on the plant.

Example 3: Evaluation of tomato plant growth promotion effect of AY001 strain

The tomato plant growth promotion effect of the AY001 strain was evaluated. To evaluate the effect of the AY001 strain on promoting tomato plant growth, 2-week-old tomato plants were treated with a suspension of the AY001 strain (2×10 ⁵ cfu/ml, 2×10 ⁷ cfu/ml) (FIG. 5). No difference was seen in plants treated with the suspension of the AY001 strain (2×10 ⁵ cfu/ml) compared to the untreated group (Figure 5, Control), but plants treated with the suspension of the AY001 strain (2×10 ⁷ cfu/ml) In plants, a growth promotion effect was observed when compared to the untreated group (Figure 5, Control). When looking at the experimental results, there was a difference as shown in Figure 5 after 2 weeks of treatment with the suspension of the AY001 strain (2×10 ⁷ cfu/ml), confirming that there was a growth promotion effect when the AY001 strain was treated on the plant.

Example 4: Evaluation of tomato bacterial black spot inhibition effect of strain AY001

In order to confirm the induced systemic resistance of the Burkholderia contaminans AY001 strain of the present invention, Pseudomonas syringae pv. tomato DC3000 ( Pst ) and tomato plants were simultaneously inoculated. Pseudomonas syringae pv. When only tomato DC3000 ( Pst ) was inoculated, bacterial black spot appeared on the plant leaves, but when simultaneously inoculated with a suspension ( ^2x107 cfu/ml) of the Burkholderia contaminans AY001 strain of the present invention, the leaves of the tomato plants showed bacterial infection. A protective effect against black spot disease was observed (Figure 6).

To confirm whether tomato bacterial black spot disease was suppressed by induced systemic resistance (ISR) of the AY001 strain, the expression of the induced systemic resistance (ISR) marker gene was confirmed. Reverse Transcription-Polymerase Chain Reaction was performed to confirm the expression of PIN2, LapA, and ACO1 genes. It was confirmed that all PIN2 , LapA , and ACO1 genes were expressed after 12 hours (Figure 7). Based on these results, it was confirmed that the AY001 strain was effective in increasing the disease resistance of tomato bacterial black spot disease in tomato plants.

In this specification and drawings, preferred embodiments of the present invention are disclosed, and although specific terms are used, these are merely used in a general sense to easily explain the technical content of the present invention and aid understanding of the present invention. There is no intention to limit the scope. It is obvious to those skilled in the art that in addition to the embodiments disclosed herein, other modifications based on the technical idea of the present invention can be implemented.

Rural Development Administration National Academy of Agricultural Sciences Microbial Bank (KACC) KACC92414P 20220418

Claims (9)

Burkholderia contaminans strain AY001 (accession number: KACC92414P).
A composition for promoting the growth of tomato plants comprising the strain according to claim 1 or a culture filtrate of the strain.
A composition for inducing systemic resistance to tomato plant pathogens, comprising the strain according to claim 1 or a culture filtrate of the strain.
According to paragraph 3,
A composition for inducing systemic resistance to a tomato plant pathogen, wherein the plant pathogen is tomato bacterial black spot disease.
A composition for controlling plant diseases for controlling peach fruit rot, pepper anthracnose, pepper late blight, cabbage sclerotia, or tomato wilt disease, comprising the strain according to claim 1 or a culture filtrate of the strain as an active ingredient.
According to paragraph 3,
The peach fruit rot is caused by Fusarium avenaceum, the pepper anthracnose is caused by Colletotrichum acutatum , and the pepper blight is caused by Phytophthora capsici . A composition for controlling plant diseases, wherein the cabbage sclerotiorum disease is caused by Sclerotinia sclerotiorum , and the tomato wilt disease is caused by Fusarium oxysporum .
A method of inducing systemic resistance to a tomato plant pathogen comprising treating a tomato plant, an area around the tomato plant, or both with the strain according to claim 1 or a culture filtrate of the strain.
In clause 7,
The resistance enhancement method is,
A method of inducing systemic resistance to tomato plant pathogens, comprising treating at least one selected from the group consisting of seeds, roots, stems, leaves, and the entire plant of the tomato plant.
A method for promoting the growth of a tomato plant comprising treating the strain according to claim 1 or a culture filtrate of the strain on a tomato plant, an area around the tomato plant, or both.