KR20230123790A - Cosmetic composition containing bio-cellulose - Google Patents

Abstract

The present invention relates to a cosmetic composition containing bio-cellulose, and specifically, contains bio-cellulose, dextran, gum karaya, gum arabic, and Mexican chia seed extract as active ingredients, and thus has excellent stability, excellent skin moisturizing and anti-aging It relates to a cosmetic composition exhibiting activity.

Description

Cosmetic composition containing bio-cellulose {Cosmetic composition containing bio-cellulose}

The present invention relates to a cosmetic composition containing bio-cellulose, and specifically, contains bio-cellulose, dextran, karaya gum, gum arabic, and Mexican chia seed extract as active ingredients, and thus has excellent formulation stability, excellent skin moisturizing and anti-aging properties. It relates to a cosmetic composition exhibiting aging activity.

The skin plays a very important role as a barrier function that protects the object from the outside. The barrier function is a protection against various stimuli from the outside, such as chemicals, air pollutants, dry environment, and ultraviolet rays, and a protective function that prevents excessive release of water in the body through the skin. Its function can be maintained only when the stratum corneum and horney layer) are normally formed. Skin is histologically composed of three layers: epidermis, dermis, and subcutaneous fat. Being the outermost of these, it plays the most important role in terms of skin aging, and thus in terms of skin beauty. It is the epidermis that has been the subject of the most intensive research.

The components that make up the stratum corneum, the outermost layer of the epidermis, are keratinocytes and skin lipids, which are responsible for the barrier function of the skin. It can be said to be an important function to protect, prevent leakage of substances in the body, and prevent evaporation of skin moisture.

However, when exposed to external environments such as stress, sunlight, air pollution, and cigarettes, reactive oxygen species are generated, lipid peroxides are generated, and skin constituent proteins such as collagen and elastin are transformed. In addition, as the activity of skin cells declines with age, the ability to produce collagen and elastin decreases. formation is promoted. In addition, as the glycosaminoglycans (GAGs) responsible for skin moisturization in the dermis layer decrease due to skin aging, the skin becomes dry, and the characteristics of aging skin such as pigmentation and loss of elasticity appear.

Human skin undergoes an aging process as its physiological function declines over time, and the indicators of aging appear in the skin. The causes of wrinkle formation among skin aging phenomena can be largely divided into wrinkles caused by natural aging and wrinkles caused by photoaging caused by exposure to ultraviolet rays. According to the research results so far, it is known that the reduction and transformation of collagen and elastin, which are the main proteins in the dermis, are directly involved in the formation of wrinkles and the decrease in skin elasticity.

In particular, collagen is a major matrix protein produced by fibroblasts of the skin, and research results have been reported that the reduction of collagen is closely related to the formation of wrinkles in the skin. In other words, free radicals that affect collagen reduction are known to induce transformation of molecules related to connective tissue formation such as skin collagen, inhibition of cell membrane function, DNA mutation promotion, protein action, transformation of energy transfer between cells, and metabolism. The fact that it is involved means that the aging process can be delayed by using antioxidants that inactivate free radicals.

In particular, since the metabolism of the skin is not smooth as aging progresses, various inflammations and allergies to the skin are aggravated. In addition, the number of people whose skin becomes sensitive due to skin irritation caused by a dry indoor environment, pollution, ultraviolet rays, and misused cosmetics or detergents is increasing. Skin that has become sensitive due to external stimuli may only have mild symptoms such as erythema, but the moisture in the stratum corneum of the skin decreases, resulting in dry skin and roughened surface, resulting in cracks. When moisture is lost, dead skin cells are easily exposed to the outside as they are eliminated, causing inflammation and skin diseases due to irritation. Dry skin weakens the immune function, making it easy to cause allergies such as itching and atopic dermatitis. Therefore, it is important to prevent water loss from the skin.

As interest in beauty and health, such as skin aging, is rapidly increasing with the improvement of living standards, research to develop functional cosmetics is being actively conducted, and retinol (vitamin A), AHA (a-hydroxy acid), adenosine, etc. are widely used as substances that increase the synthesis of collagen, but are known to be unstable to light and heat and cause irritation to the skin.

Natural polymers such as cellulose, chitosan, and polysaccharide polymers are skin-friendly materials with biocompatibility and biodegradability. Among them, cellulose is not only the most abundant biopolymer on earth, but also can be used for various purposes because it has unique physical properties depending on its type.

Cellulose commonly used in cosmetics includes cellulose microparticles having a size of several tens of micrometers, cellulose derivatives such as carboxymethyl cellulose (CMC) and hydroxyethyl cellulose (HEC), and biocellulose.

Bio-cellulose is a bio-polymer synthesized directly from microorganisms such as Acetobacter xylinum (A. xylinum), and is also called microbial cellulose or bacterial cellulose (BC). Compared to plant-derived cellulose, bio-cellulose has various advantages such as high physical strength, excellent ability to absorb and retain moisture, and formation of a uniform fiber network.

Due to these advantages, it is attracting attention as a mask sheet, but since it is mainly manufactured in a sheet form, it is difficult to apply it to various formulations. In addition, even when applied in the form of powder, it was difficult to use due to reasons such as low water dispersibility and occurrence of precipitation.

The present inventors have paid attention to the excellent skin improvement activity of bio-cellulose during research on the development of anti-aging cosmetics and have studied to apply it as a cosmetic, and tested stability by applying various ingredients. As a result, a specific ratio with bio-cellulose The present invention was completed by finding that when rhodextran, gum karaya, gum arabic, and Mexican chia seed extract were mixed and used, stability in the formulation was improved and excellent skin moisturizing and anti-aging activities were exhibited.

(0001) Republic of Korea Patent Registration No. 10-1745326 (2017.06.02.) (0002) Republic of Korea Patent Publication No. 10-2017-0060744 (2017.06.02.)

An object of the present invention is to provide a cosmetic composition containing biocellulose, dextran, gum karaya, gum arabic, and Mexican chia seed extract to improve stability and exhibit excellent skin moisturizing and anti-aging effects.

According to the present invention to achieve the above object, a cosmetic composition containing 0.1 to 10% by weight of biocellulose, dextran, gum karaya, gum arabic, and Mexican chia seed extract based on the total weight of the composition is provided.

Preferably, the bio-cellulose is characterized in that it is prepared by filtering and extracting the fermented product of Gluconacetobacter xylinus. The dextran is characterized in that it is prepared by filtering the fermentation product of Leuconostoc mesenteroides.

Preferably, the Mexican chia seed extract is characterized in that it is prepared by treating the Mexican chia seed extract with a hydrolase, more preferably β-glucosidase.

Preferably, the biocellulose, dextran, gum karaya, and gum arabic are contained in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2, respectively, and the biocellulose, dextran, gum karaya, and gum arabic The mixture is contained in a weight ratio of 1 to 5: 1 to 5 with Mexican chia seed extract, respectively.

The cosmetic composition is characterized in that it is for moisturizing the skin and improving skin wrinkles.

The cosmetic composition of the present invention containing biocellulose, dextran, gum karaya, gum arabic, and Mexican chia seed extract has excellent formulation stability and exhibits excellent skin moisturizing and anti-aging effects due to its synergistic action.

Hereinafter, the present invention will be described in more detail.

The present invention is characterized by improving the stability of bio-cellulose having excellent moisturizing activity and providing a cosmetic composition having excellent anti-aging activity such as skin wrinkle improvement as well as excellent moisturizing activity through mixed use with other active ingredients. do.

Bio-cellulose included as an active ingredient in the cosmetic composition of the present invention can be obtained by a conventional method in the art, and its preparation method is not particularly limited. The bio-cellulose of the present invention is preferably prepared by filtering and extracting the fermented product of Gluconacetobacter xylinus (Acetobacter xylinus), A. pasteurianus ( A. pasteurinanus ), Acetobacter hansenii ( A. hansenii ), as well as other Acetobacter sp. microorganisms such as Agrobacterium sp. , Rhizobium sp. , Pseudomonas sp. , Sarcina sp. Genus microorganisms and the like can also be used. The carbon source of the microorganism to be inoculated is well known in the art, and therefore, any material known in the art may be used, usually oligosaccharide, lactose, glucose, fructose, sucrose, molasses, dextrose, mixtures thereof, and the like may be used. . In addition, various natural extracts such as plants may be mixed with the culture medium to improve skin cosmetic functional ingredients or impart flavoring and/or coloring effects.

Dextran, which is included as another active ingredient of the cosmetic composition of the present invention, is a natural polymer produced by Leuconostoc strains and is used as a thickener, binder, and bulking agent in cosmetic formulations. It can be obtained by a conventional method in the art, and the manufacturing method is not particularly limited. In the present invention, it is preferably prepared by filtering the fermented product of Leuconostoc mesenteroides .

Karaya gum, another active ingredient of the cosmetic composition of the present invention, is dried gummy secretion of Sterculia urens Roxburgh, and is a polysaccharide mainly composed of galactose, rhamnose and galacturonic acid. It is used as an emulsifier, thickener, stabilizer, etc. The pH of a 1% (w/v) aqueous solution is 4.5-5.0.

Gum arabic (Acacia Senegal Gum), which is an active ingredient of the cosmetic composition of the present invention, is also named acacia Senegal gum, and what is not pulverized is white-yellowish-white round lumps of various sizes, angular pieces, or white-yellowish-white It is in the form of flakes, granules or powder. It is obtained by drying or desalting the secretion of black arabic beans, Acacia senegal WILLDENOW or other plants of the same genus, and the main component is polysaccharide. When water is added, it dissolves slowly and shows acidity. It becomes a tasteless thick viscous liquid up to 50% aqueous solution, and the viscosity decreases over time. In particular, stable emulsions can be obtained in a wide range of pH, and o/w emulsification is possible even in the presence of electrolytes such as table salt.

Mexican chia seeds (Salvia hispanica seeds) are the seeds of chia (Salvia hispanica), which is a species of flowering plant belonging to the mint family native to southern central Mexico and Guatemala. It is cultivated for its seeds and has been a common food and nutraceutical for centuries in South America, western Mexico, and the American Southwest. Mexican chia seeds have hydrophilic properties and are capable of absorbing about 12 times as much water. Looking at the nutritional content per 100 g of Mexican chia seeds, it contains about 42 g of carbohydrates, 30 g of fat, 16 g of protein, and other minerals, and contains about 25-30% of omega-3 fatty acids such as linolenic acid. In addition, Mexican chia seeds contain a relatively high protein content, unlike other grains such as wheat, corn, rice, and oats.

According to the present invention, a cosmetic composition containing 0.1 to 10% by weight of bio-cellulose, dextran, gum karaya, gum arabic, and Mexican chia seed extract based on the total weight of the composition is provided.

Preferably, the bio-cellulose is produced by filtration and extraction of fermented product of Gluconacetobacter xylinus . In addition, the dextran is characterized in that it is prepared by filtering the fermentation product of Leuconostoc mesenteroides .

The Mexican chia seed extract is prepared by treating Salvia hispanica Seed extract with a hydrolase, more preferably β-glucosidase. In this case, it exhibits more excellent moisturizing and anti-wrinkle activity.

The biocellulose, dextran, gum karaya, and gum arabic are contained in a weight ratio of 1 to 2: 1 to 2: 1 to 2: 1 to 2, respectively, and are contained in a weight ratio of 2: 1: 1: 2, respectively It is preferable because it has more excellent stability, skin moisturizing, and anti-wrinkle activity of bio-cellulose.

In addition, the mixture of biocellulose, dextran, gum karaya, and gum arabic has a weight ratio of 1 to 5: 1 to 5, more preferably 1: 5, respectively, with the Mexican chia seed extract, which is another active ingredient. is contained with

It was confirmed that the stability of biocellulose in the formulation was excellent when it was contained in the above composition and ratio (Test Example 7). In addition, the cosmetic composition has an excellent hyaluronic acid synthase (HAS-3) expression increasing effect (Test Example 3), hyaluronidase activity inhibitory effect (Test Example 4), MMP-1 production inhibitory effect (Test Example 5) , showed collagen biosynthesis promoting effect (Test Example 6).

The cosmetic composition of the present invention can be prepared in any formulation that is conventionally prepared, and examples thereof include lotion, cream, essence, cleansing foam, cleansing water, pack, body lotion, body oil, body gel, shampoo, rinse, and hair. Conditioner, hair gel, foundation, lipstick, mascara, makeup base, etc. may be mentioned.

[Example]

Hereinafter, the present invention will be described in detail through the following examples and test examples, but the present invention is not limited by these examples.

Preparation Example 1: Preparation of bio-cellulose

A nutrient medium (1L) was sterilized with the composition shown in Table 1 below.

Furtherance content yeast extract 5g/L bacto peptone 3g/L mannitol 25g/L glycerol 2g/L Coconut Juice 5g/L Distilled water balance

Gluconacetobacter xylinus strain was inoculated into a sterilized medium in an amount of OD600 = 0.1, and then cultured for 10 days in a constant temperature incubator at 30°C. The stationary culture solution containing the microbial cellulose produced from the Gluconacetobacter strain was centrifuged at 3,000 rpm for 30 minutes to remove the supernatant, and a 0.3N NaOH solution was added thereto. dissolved in NaOH solution. After removing the NaOH solution by centrifugation at 4,000 rpm for 20 minutes, a 5% acetic acid solution was added and washed. After removing the acetic acid solution by centrifugation at 4,000 rpm for 20 minutes, it was neutralized by washing with a sufficient amount of distilled water and dried in an oven at 65 ° C until there was no change in weight. The resulting cellulose was pulverized to prepare biocellulose powder.

Preparation Example 2: Preparation of Dextran

Nutrient medium (1L) of the composition shown in Table 2 below was sterilized.

Furtherance content bactopeptone 10g/L beef extract 10g/L yeast extract 5g/L glucose 20g/L Dibasic Potassium Phosphate 2g/L Coconut Juice 5g/L glycerol 2g/L twin 80 1g/L Ammonium Citrate 2g/L sodium acetate 5g/L manganese sulfate 0.1g/L magnesium sulfate 0.05g/L Distilled water balance

Leuconostoc mesenteroides was inoculated into a sterilized medium in an amount of OD600 = 0.1 and inoculated and cultured for 10 days in a constant temperature incubator at 30 ° C. The stationary culture solution was centrifuged at 3,000 rpm for 30 minutes to remove the supernatant and dried to prepare dextran.

Examples 1 to 5: Preparation of mixtures of biocellulose, dextran, gum karaya and gum arabic

Biocellulose and dextran prepared in Preparation Examples 1 and 2, gum karaya (Naturganic, produced in India), and gum arabic (ES food raw material, produced in France) were mixed in the weight ratio shown in Table 3 below to obtain a mixture. (100 g) was prepared.

weight ratio bio cellulose Dextran karaya sword gum arabic Example 1 One One One One Example 2 One 2 2 One Example 3 2 One 2 2 Example 4 2 2 One One Example 5 2 One One 2

Preparation Example 3: Preparation of Mexican Chia Seed Extract

After washing with purified water, add 5 times the weight of 70% water-containing ethanol as an extraction solvent to 1Kg of dried Mexican chia seeds and heat them at 80-100℃ in an extractor with a cooling condenser (Cosmos-660, Gyeongseo Machinery) for 2 hours in total. Extracted twice.

After extraction by the above method, the precipitate was left at room temperature for 3 days, and the precipitate was filtered with 300 mesh filter paper, and the precipitate was filtered twice with Edvantech No. 5 filter paper and Whatman GFC 150mm filter paper. After concentrating at a temperature of 40℃~50℃ using a vacuum concentrator (Coolace CCA-1100, EYELA), use a spray dryer (B-290, BUCHI) at an inlet temperature of 180℃ and an aspirator. Drying under conditions of 100% efficiency (35cm3/hour), 25% pump efficiency (7.5ml/minute), Nozzle Cleaner 4 and Rotameter: 30mm (357 liters/hour) to obtain 100 g of the titled extracted powder.

Preparation Example 4: Preparation of enzyme-treated Mexican chia seed extract

10 g of the extracted powder prepared in Preparation Example 3 was added to 500 ml of acetate buffer (pH 4.0) and 1 ml of β-glucosidase enzyme and reacted at 50° C. for 20 hours. Next, after heating to 95 ℃ for 30 minutes to complete the enzymatic reaction, the reactant was concentrated in vacuo. The concentrate was dried at 60 °C for 30 hours. After extraction by the above method, the precipitate was left at room temperature for 3 days, and the precipitate was filtered with 300 mesh filter paper, and the precipitate was filtered twice with Edvantech No. 5 filter paper and Whatman GFC 150mm filter paper. After concentrating at a temperature of 40℃~50℃ using a vacuum concentrator (Coolace CCA-1100, EYELA), use a spray dryer (B-290, BUCHI) at an inlet temperature of 180℃ and an aspirator. Efficiency 100% (35cm3/hour), Pump efficiency 25% (7.5ml/min), Nozzle Cleaner 4 and Flowmeter Rotameter: Dry under conditions of 30mm (357 liters/hour) An enzyme-treated Mexican chia seed extract was prepared.

Test Example 1: Confirmation of the effect of increasing the expression of hyaluronic acid synthase (HAS-3)

Human fibroblast NHDF was adjusted to 5×10 ⁵ cells/well using DMDM medium supplemented with 10% FBS, and then inoculated into a 12 well plate and cultured for 18 hours. After culturing, the samples of Examples 1 to 5 and Preparation Example 4 were treated and cultured for 24 hours. RNA was extracted from the cultured cells and the increase in expression of Hyaluronan Synthase-3 (HAS-3) was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The HAS-3 expression increase rate was calculated by the following formula, and the results are shown in Table 4.

HAS-3 expression increase rate (%) = (sample HAS-3 expression amount / control group HAS-3 expression amount) × 100

division HAS-3 increase rate (%) 0.5% 1.5% 2 % Example 1 40.2 55.2 58.2 Example 2 42.5 56.5 59.2 Example 3 43.5 57.8 60.2 Example 4 44.5 54.2 56.2 Example 5 50.2 61.0 62.5 Production Example 4 38.3 50.7 51.3

Among the mixture samples, it was confirmed that the sample of Example 5 had the most excellent effect of increasing the expression of hyaluronic acid synthase (HAS-3).

Examples 6 to 8: Preparation of Complexes of Biocellulose, Dextran, Karaya Gum and Arabic Gum Mixtures and Mexican Chia Seed Extract

A composite was prepared by mixing the mixture of Example 5, which exhibits the highest hyaluronic acid synthase expression increasing effect, and the enzyme-treated Mexican chia seed extract of Preparation Example 4 in a weight ratio shown in Table 5 below.

weight ratio Example 5 Preparation Example 4 Example 6 One One Example 7 5 One Example 8 One 5

Comparative Example 1: Preparation of Complex of Biocellulose, Dextran, Karaya Gum and Arabic Gum Mixture and Mexican Chia Seed Extract

A composite was prepared by mixing the mixture of Example 5 and the Mexican chia seed extract of Preparation Example 3 at a weight ratio of 1:5, which exhibited the highest hyaluronic acid synthase expression increasing effect.

Test Example 2: Confirmation of cytotoxicity

In order to confirm cytotoxicity, fibroblasts were inoculated at a cell concentration of 5×10 ⁵ in IMDM medium supplemented with 10% FBS, and cultured for 24 hours in a 37° C. 5% CO ₂ incubator. [ 4,5-dimethylthiazol-2yl] -2,5-diphenyltetrazolium boromide, Sigma, USA) solution was added to each well at 100 ml (3 mg/ml), followed by further incubation for 4 hours. Thereafter, the supernatant was removed, 150 μl of dimethyl sulfoxide was added, shaken for 30 minutes to dissolve the generated formazan, and absorbance was measured at 540 nm using a multimicroplate reader (Molecular device Spectra max190). Cell viability was calculated according to the formula below, and the results are shown in Table 6 below.

Cell viability (%) = absorbance of sample added group / absorbance of control group × 100

division Cell viability (%) 0.5% 1.5% 2 % Example 1 100 101 100 Example 2 100 101 100 Example 3 100 100 100 Example 4 101 100 100 Example 5 100 100 101 Example 6 100 101 100 Example 7 100 100 101 Example 8 100 101 100

As confirmed in Table 6, it was confirmed that all samples did not affect cytotoxicity, and thus there was no problem with safety.

Test Example 3: Confirmation of the effect of increasing the expression of hyaluronic acid synthase (HAS-3)

Human fibroblast NHDF was adjusted to 5×10 ⁵ cells/well using DMDM medium supplemented with 10% FBS, and then inoculated into a 12 well plate and cultured for 18 hours. After culturing, the samples of Examples 6 to 8 and Comparative Example 1 were treated and cultured for 24 hours. RNA was extracted from the cultured cells and the increase in expression of Hyaluronan Synthase-3 (HAS-3) was confirmed through reverse transcription polymerase chain reaction (RT-PCR). The HAS-3 expression increase rate was calculated by the following formula, and the results are shown in Table 7. As a control, hyaluronic acid was used.

HAS-3 expression increase rate (%) = (sample HAS-3 expression amount / control group HAS-3 expression amount) × 100

division HAS-3 increase rate (%) 0.5% 1.5% 2 % Comparative Example 1 56.3 62.6 67.2 Example 6 58.5 59.5 62.4 Example 7 59.2 62.2 64.3 Example 8 75.5 78.0 80.2 Hyaluronic Acid 73.2 78.2 81.2

As shown in Table 7, in the composites of Examples 6 to 8, the mixture of Examples 1 to 5, the enzyme-treated Mexican chia seed extract of Preparation Example 4 (Table 4), or the non-enzyme-treated Mexican chia seeds Compared to Comparative Example 1, which is a composite using the extract, it was confirmed that the effect of increasing the expression of hyaluronic acid synthetase (HAS-3) was excellent. In particular, the complex of Example 8 exhibited an effect of increasing expression equivalent to that of the control hyaluronic acid.

Test Example 4: Confirmation of the activity inhibitory effect of hyaluronidase

The effect of inhibiting hyaluronidase activity was confirmed as follows by applying the Morgan-Elson method. The final enzyme activity of hyaluronidase was 400 NF unit/ml, the final concentration of HA was 0.4 mg/ml, and the active compound 48/80 buffer solution (0.1 mg/ml) was used to prepare inactive hyaluronic acid. Hyaluronidase activity was measured focusing on the inhibition of the activation step of the enzyme. The sample was dissolved in a buffer solution to form a sample solution, and the control group used the buffer solution instead of the sample solution. As a control, hyaluronic acid was used. The activity inhibition rate (%) of hyaluronidase was calculated by the following formula, and the results are shown in Table 8.

Hyaluronidase activity inhibition rate (%) = [(A-B) / A] × 100

A: Enzyme activity of wells to which no sample was added

B: enzyme activity of the well to which the sample was added

division Hyaluronidase activity inhibition rate (%) 0.5% 1.5% 2 % Example 1 34.2 36.1 54.6 Example 2 28.7 37.4 50.3 Example 3 22.5 34.2 42.2 Example 4 31.2 34.5 54.8 Example 5 29.3 32.2 57.3 Production Example 4 21.2 30.1 42.3 Comparative Example 1 50.3 59.2 65.9 Example 6 51.8 61.2 62.3 Example 7 53.4 55.7 61.1 Example 8 71.8 77.6 76.5 Hyaluronic Acid 72.1 72.2 74.4

The composites of Examples 6 to 8 exhibited an excellent hyaluronidase activity inhibitory effect compared to the mixtures of Examples 1 to 5 or the enzyme-treated Mexican chia seed extract of Preparation Example 4, and thus the synergistic effect of mixing I was able to confirm. In addition, it was confirmed that the hyaluronidase activity inhibition effect was superior to that of the composite using the Mexican chia seed extract without enzyme treatment (Comparative Example 1).

Test Example 5: Confirmation of MMP-1 production inhibitory effect

Fibroblasts, which are normal human skin cells (Korea Cell Line Bank, Korea), were inoculated into 48-well microplates (Nunc. Denmark) to be 1st 10 ⁶ cells per well, and DMEM medium (Sigma, United States of America) and 37°C conditions. After incubation for 24 hours, the concentration of the extracts of Preparation Examples and Examples was diluted in dimethyl sulfoxide to a concentration of 0.5%, 1.5%, and 2%, and then diluted solutions were added, followed by serum-free DMEM medium for 48 hours. cultured. After culturing, the supernatant of each well was collected and the amount (μg/ml) of newly synthesized MMP-1 was measured using an MMP-1 assay kit (Amersham, USA). As a positive control for the inhibition of MMP-1 production, TGF-β (10 mg/ml, Roche, USA) was used. The MMP-1 production inhibition rate was calculated according to the following formula, and the results are shown in Table 9 below.

MMP-1 production inhibition rate (%) = [1-(B/A)] x 100

A: amount of MMP-1 in the control group

B: Amount of MMP-1 in the experimental group

sample concentration
(μg/ml) MMP-1 production inhibition rate (%) 0.5% 1.5% 2 % Example 1 21.7 31.2 44.5 Example 2 22.2 31.8 45.7 Example 3 28.7 35.7 48.4 Example 4 32.9 36.4 50.3 Example 5 27.5 43.4 50.2 Production Example 4 26.5 32.5 40.3 Comparative Example 1 31.2 38.7 45.6 Example 6 33.2 47.2 67.0 Example 7 37.4 49.9 73.5 Example 8 45.4 76.5 92.1 TGF-β 48.5 77.6 94.6

As confirmed in Table 9, the composites of Examples 6 to 8 exhibited an excellent inhibitory effect on MMP-1 production compared to the mixtures of Examples 1 to 5 or the enzyme-treated Mexican chia seed extract of Preparation Example 4. 8 showed the best MMP-1 production inhibitory effect.

Test Example 6: Confirmation of collagen biosynthesis promoting effect

Fibroblasts were inoculated at a cell concentration of 5×10 ⁵ in IMDM medium supplemented with 10% FBS, and cultured for 24 hours in a 37° C., 5% CO ₂ incubator. After culturing for 24 hours, dilute solutions prepared by diluting the extracts of Preparation Examples and Examples in dimethyl sulfoxide to a concentration of 0.5, 1.0, and 2.0%, respectively, were added, cultured under the same conditions for 18 hours, and then irradiated with UV for 1 hour. to stress the cells. Thereafter, fibroblasts were recovered using Trizol reagent (Invitrogen, USA), mRNA was extracted, and cDNA was synthesized through a series of processes. The PROCOLLAGEN TYPE I gene region was amplified from the synthesized cDNA, and the gene expression level was confirmed through electrophoresis. Gene amplification was performed using a thermal cycler (GenePro, Hangzhou bioer tech., CHINA) in 10x taq polymerase buffer, 10 mM dNTP, 10 Mix pmol primer (F: AGC CAG CAG ATC GAG AAC AT, R: TCT TGT CCT TGG GGT TCT TG) and taq polymerase, add distilled water to adjust to 50 uL, and 95 degrees 5 minutes 1 cycle/95 degrees 1 minute/51 degrees 2 minutes / 72 degrees 1 minute 28 cycle amplification, was carried out under the condition of reacting at 72 degrees for 5 minutes. The expression rate of the produced procollagen was calculated according to the following formula, and as a control group, normal fibroblasts not treated with UV light were used.

procollagen expression rate (%) = B/A x 100

A: the amount of procollagen expression in the control group

B: amount of procollagen expression in sample treatment and UV-irradiated fibroblasts

Sample concentration (%) procollagen expression rate 0.5% 1.5% 2 % Example 1 31.6 45.2 57.4 Example 2 34.2 38.7 44.4 Example 3 45.6 48.4 58.3 Example 4 40.0 55.1 70.1 Example 5 40.3 58.7 63.0 Production Example 4 38.8 47.1 51.8 Comparative Example 1 35.2 49.8 55.2 Example 6 71.2 82.2 87.3 Example 7 79.6 86.8 91.2 Example 8 144.3 150.5 165.7

As confirmed in Table 10, it was confirmed that the composites of Examples 6 to 8 exhibited an excellent collagen biosynthesis promoting effect, and the composite sample of Example 8 exhibited a particularly excellent collagen biosynthesis promoting effect.

Formulation Example 1: Preparation of skin lotion

According to the composition of Table 11 below, 1 kg of skin lotion containing the complex of the examples of the present invention was prepared in a conventional manner.

Raw material Content (% by weight) Composite (Example 8) 1.0 glycerin 3.0 Butylene Glycol 2.0 propylene glycol 2.0 Polyoxyethylene Hydrogenated Castor Oil 1.0 ethanol 10.0 triethanolamine 0.1 antiseptic a very small amount pigment a very small amount Spices a very small amount Purified water balance

Formulation Example 2: Preparation of nutritional lotion

According to the composition of Table 12 below, 1 kg of nutritional lotion containing the complex of Examples of the present invention was prepared in a conventional manner.

Raw material Content (% by weight) Composite (Example 8) 1.0 beeswax 1.0 Polysorbate 60 1.5 Sorbitan Sesquioleate 0.5 liquid paraffin 10.0 Sorbitan Stearate 1.0 Pro-type glycerin monostearate 0.5 stearic acid 1.5 Glyceryl Stearate/PEG-400 Stearate 1.0 propylene glycol 3.0 carboxypolymer 0.1 triethanolamine 0.2 antiseptic a very small amount pigment a very small amount Spices a very small amount Purified water balance

Test Example 7: Confirmation of Formulation Stability

Storage and observation for 12 weeks ( discoloration, odor and separation), and stability was confirmed. The results are shown in Table 13.

temperature condition Stability check (discoloration, odor and separation) Formulation Example 1 Formulation Example 2 Room temperature (25℃) 0 0 Refrigeration (4℃) 0 0 constant temperature (50℃) 0 0

<Formulation Stability Grade>

0: No change 1: Minor change 2: Change 3: Extreme change

As confirmed in Table 13, it can be seen that the formulation of the skin lotion and nutrient lotion containing the biocellulose powder did not show discoloration, odor, or separation even after 12 weeks of storage, indicating excellent stability.

Claims (6)

A cosmetic composition containing 0.1 to 10% by weight of biocellulose, dextran, gum karaya, gum arabic, and Mexican chia seed extract based on the total weight of the composition. The method of claim 1, wherein the biocellulose is prepared by filtration and extraction of fermented product of Gluconacetobacter xylinus , and the dextran is obtained by filtering the fermented product of Leuconostoc mesenteroides . A cosmetic composition, characterized in that manufactured. The cosmetic composition according to claim 1, wherein the Mexican chia seed extract is prepared by treating the Mexican chia seed extract with a hydrolase. The method of claim 1, wherein the biocellulose, dextran, gum karaya, and gum arabic are contained in a weight ratio of 1 to 2:1 to 2:1 to 2:1 to 2, respectively, and the biocellulose, dextran, gum karaya and a mixture of gum arabic and Mexican chia seed extract in a weight ratio of 1 to 5:1 to 5, respectively. The cosmetic composition according to claim 1, wherein the cosmetic composition is for moisturizing the skin. The cosmetic composition according to claim 1, wherein the cosmetic composition is for improving skin wrinkles.