KR20230091032A - Novel Bacillus megaterium strainand use thereof - Google Patents

Abstract

The present invention includes a Bacillus megaterium strain deposited under deposit number KCTC14778BP, and a cosmetic composition, quasi-drug composition, or food composition containing at least one selected from a group consisting of the strain, a culture of the strain, and a fermented product of the strain. According to the present invention, since the Bacillus megaterium strain, the culture of the strain, or the fermentation product of the strain has excellent skin improvement effects, the Bacillus megaterium strain has high utility as cosmetic compositions, quasi-drug compositions, and food compositions for skin improvement. Accordingly, various industrial applications such as cosmetics, quasi-drugs, food, and the like can be expected.

Description

Novel Bacillus megaterium strain and use thereof {Novel Bacillus megaterium strain and use thereof}

The present invention is a Bacillus megaterium strain deposited with Accession No. KCTC14778BP; And to a cosmetic composition, quasi-drug composition, or food composition comprising at least one selected from the group consisting of the strain, culture of the strain, and fermented product of the strain.

Cosmetic ingredients are generally known to cause various skin problems such as inflammation, pimples, and edema (Maibach. H. I., Contact Dermatitis, 6. 369-404, 1980). In addition, various materials used as raw materials for functional cosmetics also have a problem of low stability or insignificant effect on the skin.

In general, moisturizing cosmetic compositions used for the skin maintain a certain amount of moisture in human hair or skin to make them look soft and lively, and function to prevent damage such as cracks and dryness. That is, the cosmetic composition for moisturizing the skin is used for the purpose of beautifying the skin or hair and keeping them healthy by supplying a certain amount or more of moisture to the skin or hair or keeping the moisture.

Therefore, in recent years, a lot of research has been conducted on a cosmetic composition having an excellent moisturizing effect in terms of improving skin conditions or preventing skin aging or skin damage.

Under this background, the present inventors have conducted research on raw materials that are safer and have excellent skin improvement effects from natural resources, and the new strain of Bacillus megaterium has a skin improvement effect and improves skin compared to conventional Bacillus fermented products. The present invention was completed by confirming that the effect was remarkably excellent.

One object of the present invention is to provide a Bacillus megaterium strain deposited under accession number KCTC14778BP.

Another object of the present invention is to provide a cosmetic composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

Another object of the present invention is to provide a quasi-drug composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

Another object of the present invention is to provide a food composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

One object of the present invention is to provide a method for producing a fermented product, including the step of fermenting with a Bacillus megaterium strain deposited under Accession No. KCTC14778BP.

Hereinafter, the contents of the present invention will be described in detail. On the other hand, the description and embodiment of one aspect disclosed in the present invention can also be applied to the description and embodiment of another aspect with respect to common matters. Additionally, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be seen that the scope of the present invention is limited by the specific descriptions described below.

One aspect of the present invention provides a Bacillus megaterium strain deposited under accession number KCTC14778BP.

The term of the present invention, "Bacillus megaterium (Bacillus subtilis, Priestia megaterium)" is a rod-shaped Gram-positive aerobic spore-forming bacterium mainly found in various habitats. It refers to a species of bacillus belonging to the genus Bacillus and Priestia. It is widely used as a feed additive for poultry, livestock, aquatic animals, and ruminants. Bacillus megaterium has different characteristics depending on the growth area and natural environment conditions, so the activity of the strain and its fermentation product may vary, and the Bacillus megaterium of the present invention may be derived from a fermentation broth, but is not limited thereto.

In the present invention, the fermentation broth may be fermented for 6 months to 2 years, specifically for 9 months to 1 year and 6 months, more specifically for 1 year to prepare a plant fermentation broth, and the fermentation broth may be a plant fermentation broth, but is not limited thereto.

In one embodiment of the present invention, a fermented liquid was prepared, and it was confirmed that the fermented product of Bacillus megaterium of the present invention had an excellent skin improvement effect compared to the normal fermented product of Bacillus.

Another aspect of the present invention provides a cosmetic composition for improving skin comprising any one or more of the strain, culture of the strain, and fermented product of the strain.

As used herein, the term "strain" refers to a collection of individuals derived from a single cell, propagated through asexual reproduction, and having homogeneous genetic characteristics, and within one species, genetic characteristics Several different strains (genetic variants) exist. In the present invention, the strain refers to Bacillus megaterium, and may specifically be Bacillus megaterium deposited under accession number KCTC14778BP, but is not limited thereto.

In the present invention, the strain may include cells, dried cells, lysates, and the like of Bacillus megaterium strains. At this time, the dried cells may be spray-dried bacteria, freeze-dried bacteria, vacuum-dried bacteria, drum-dried bacteria, etc., and the lysate is a product obtained by breaking the cell walls of the strain itself by chemical or physical force. can be

The "culture" of the present invention means a product obtained by culturing the strain in a medium. For example, the culture of the present invention may include components remaining in the medium remaining after harvesting the strain from the culture medium of Bacillus megaterium.

The culture may be a whole culture, a culture supernatant, a lysate, a fraction thereof, or the like of the Bacillus megaterium strain. At this time, the culture supernatant can be obtained by centrifuging the culture of the strain, the lysate can be obtained by physically or sonicating the strain, and the fraction is the culture, culture supernatant, lysate, etc. It can be obtained by applying methods such as centrifugation and chromatography.

As the medium and other culture conditions used for culturing the strain of the present invention, any medium may be used without particular limitation as long as it is a medium used for culturing conventional microorganisms of the genus Bacillus. , It can be cultured while controlling temperature, pH, etc. under aerobic or anaerobic conditions in a conventional medium containing inorganic compounds, amino acids, and/or vitamins.

Examples of the carbon source in the present invention include carbohydrates such as glucose, fructose, sucrose, and maltose; sugar alcohols such as mannitol and sorbitol; organic acids such as pyruvic acid, lactic acid, citric acid and the like; Amino acids such as glutamate, methionine, lysine, and the like may be included, but are not limited thereto. In addition, natural organic nutritional sources such as starch hydrolysate, molasses, blackstrap molasses, rice winter, cassava, sorghum and corn steep liquor can be used, as well as glucose and pasteurized pretreated molasses (i.e., molasses converted to reducing sugars). Carbohydrates such as may be used, and various other carbon sources in appropriate amounts may be used without limitation. These carbon sources may be used alone or in combination of two or more.

Examples of the nitrogen source include inorganic nitrogen sources such as ammonia, ammonium sulfate, ammonium chloride, ammonium acetate, ammonium phosphate, ammonium carbonate, and ammonium nitrate; Organic nitrogen sources such as amino acids, peptones, NZ-amines, meat extracts, yeast extracts, malt extracts, corn steep liquor, casein hydrolysates, fish or degradation products thereof, defatted soybean cakes or degradation products thereof, and the like can be used. These nitrogen sources may be used alone or in combination of two or more, but are not limited thereto.

The number of persons may include monopotassium phosphate, dipotassium phosphate, or a sodium-containing salt corresponding thereto. As the inorganic compound, sodium chloride, calcium chloride, iron chloride, magnesium sulfate, iron sulfate, manganese sulfate, calcium carbonate, and the like may be used.

In addition, amino acids, vitamins, and/or appropriate precursors may be included in the medium. Specifically, L-amino acids and the like may be added to the culture medium of the strain. Specifically, glycine, glutamate, and/or cysteine may be added, and if necessary, L-amino acids such as lysine may be further added, but it is not necessarily limited thereto. don't

The medium or precursor may be added to the culture in a batch or continuous manner, but is not limited thereto.

In the present invention, the pH of the culture can be adjusted by adding compounds such as ammonium hydroxide, potassium hydroxide, ammonia, phosphoric acid, sulfuric acid and the like to the culture in an appropriate manner during cultivation of the strain. In addition, during cultivation, the formation of bubbles can be suppressed by using an antifoaming agent such as a fatty acid polyglycol ester. In addition, in order to maintain the aerobic state of the culture, oxygen or oxygen-containing gas may be injected into the culture, or nitrogen, hydrogen or carbon dioxide gas may be injected without injection of gas or gas to maintain the anaerobic and non-aerobic state.

The temperature of the culture may be 25 °C to 40 °C, more specifically 28 °C to 37 °C, but is not limited thereto. The culturing period may be continued until a desired production amount of a useful substance is obtained, and specifically may be 1 hour to 200 hours, but is not limited thereto.

In the present invention, the term "fermentation" refers to a process in which microorganisms decompose organic matter using their own enzymes, which is not a decay reaction. Fermentation and decay reactions proceed by similar processes, but when useful substances are produced as a result of decomposition, it is called fermentation, and when bad smells or harmful substances are produced, it is called decay.

In the present invention, the method for obtaining the fermented product from the strain is not particularly limited, and the strain is inoculated into a medium, shaken cultured at 20 ° C to 40 ° C for 1 hour to 200 hours, and then the cells are separated by centrifugation, A fermented product can be obtained by a method of disrupting the cells by ultrasonication. Alternatively, it can be obtained according to a method commonly used in the art or similar fields.

In the present invention, the type of medium in which the strain is inoculated is not limited as long as the strain can proceed with fermentation.

In the present invention, the fermentation product obtained from the strain is not only the fermented material itself, but also the culture medium of the strain in which the strain and the culture coexist, the fermentation product obtained by filtering the strain from the culture medium, and the strain from the culture medium. A fermented product obtained by filtering it, an extract obtained by extracting the fermented product or a culture medium containing the same, a diluted solution obtained by diluting the fermented product or its extract, a dried product obtained by drying the fermented product or its extract, and a cell body of the strain collected and crushed Lysates, etc., include all kinds of materials including fermented products generated from the above strains.

The term of the present invention, "skin improvement" may be skin moisturizing, but is not limited thereto.

As used herein, the term "improvement" refers to any activity that at least reduces a parameter associated with alleviation or treatment of a condition, for example, the severity of a symptom, and may include skin moisturizing.

The skin improvement may be achieved through promotion of hyaluronic acid production, but is not limited thereto.

Specifically, the present inventors confirmed that the fermented product of Bacillus megaterium KCTC14778BP of the present invention has an excellent skin improvement effect compared to the fermented product of general Bacillus. More specifically, it was confirmed that the fermented product of Bacillus megaterium KCTC14778BP of the present invention was excellent in promoting hyaluronic acid synthesis. Therefore, it was confirmed that the Bacillus megaterium or fermented product of the present invention has superior skin improvement effects such as skin moisturizing compared to general Bacillus or fermented product thereof.

As used herein, the term "moisturizing the skin" refers to all actions that maintain a smooth surface by supplying moisture to the skin or blocking evaporation of moisture to maintain the flexibility of the skin and inducing uniform exfoliation of dead skin cells, and "drying" means a condition in which such skin moisturizing is not sufficient. The skin moisturizing effect can help improve wrinkles and increase elasticity of the skin. Specifically, skin moisturizing may be due to promotion of hyaluronic acid production, but is not limited thereto.

As used herein, the term "hyaluronic acid" is a type of glycosaminoglycans, and refers to a chain-like polymeric polysaccharide material in which glucuronic acid and N-acetylglucosamine residues are repeatedly linked. Hyaluronic acid can contain water equivalent to 300 to 1000 times its own weight, and exhibits a moisturizing effect on the skin by combining with moisture.

In one embodiment of the present invention, it was confirmed that the Bacillus megaterium fermented product of the present invention had an excellent hyaluronic acid synthesis enhancement rate, and through this, the Bacillus fermented product of the present invention had a remarkable effect on skin moisturizing, etc. (Table 2) .

The term "cosmetic composition" as used herein refers to solutions, external ointments, creams, foams, nourishing lotions, softening lotions, perfumes, packs, softening waters, emulsions, makeup bases, essences, soaps, liquid cleansers, bath additives, sunscreen creams, It may be a formulation selected from the group consisting of sun oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray. , but not limited thereto.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as typical ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, A chelating agent, a colorant, a preservative, a flavoring agent, and the like may be suitably blended, but is not limited thereto.

Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary according to the formulation of the cosmetic composition.

When the cosmetic formulation of the present invention is an ointment, paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanthate, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, oxide as a carrier component Zinc or the like may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier components. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and in particular, in the case of a spray, chloro propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

On the other hand, all of the ingredients described in the present invention may preferably be included in the composition of the present invention within the range of not exceeding the maximum value specified in the regulations on cosmetic safety standards and China's 'cosmetic safety and technical regulations'.

Another aspect of the present invention provides a quasi-drug composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

The "Bacillus megaterium", "strain", "culture", "fermented product", "skin improvement", "improvement", "skin moisturizing" and "hyaluronic acid" are as described above.

The term "quasi-drugs" of the present invention refers to body cleansers, disinfectant cleaners, detergents, kitchen cleaners, cleaning cleaners, toothpaste, gargles, wet wipes, detergents, soaps, hand washes, hair cleaners, hair softeners, humidifier fillers, masks, ointments, and filters It may be selected from the group consisting of fillers, but is not limited thereto.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent as needed in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, aromatics, preservatives, and the like. can include

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , injectable esters, Witepsol, Macrogol, Tween 61, cacao butter, laurage, and the like.

When the culture of the strain of the present invention or its fermented product is used as a quasi-drug, it may additionally contain one or more active ingredients exhibiting the same or similar functions. For example, it may include known skin beneficial bacteria enhancement, skin harmful bacteria suppression, skin soothing, skin inflammation improvement, skin whitening, skin regeneration, and wound healing components. If additional wrinkle improvement, skin whitening, skin trouble improvement, and skin moisturizing components are included, the skin moisturizing effect of the composition of the present invention can be further increased. When adding the above components, skin safety according to combined use, ease of formulation, and stability of active ingredients may be considered.

The quasi-drug composition of the present invention is a skin moisturizing ingredient known in the art, retinoic acid, TGF, animal placenta-derived protein, betulinic acid and chlorella extract; As anti-inflammatory components known in the art, non-steroidal flufenamic acid, ibuprofen, benzidamine, indomethacin, prednisolone, dexamethasone, allantoin, azene, hydrocortisone; And it may further include one or two or more components selected from the group consisting of derivatives thereof and various plant extracts. Additional components may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range depends on requirements such as skin safety, ease of formulation of the strain, culture or fermented product of the present invention. will be adjustable.

Formulation method, dosage, usage method, components, etc. of quasi-drugs can be appropriately selected from conventional techniques known in the art.

Another aspect of the present invention provides a food composition for improving skin comprising any one or more of the strain, the culture of the strain, and the fermented product of the strain.

The "Bacillus megaterium", "strain", "culture", "fermented product", "skin improvement", "improvement", "skin moisturizing" and "hyaluronic acid" are as described above.

The term "food" of the invention means meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, vitamins It includes all food in the usual sense, such as complexes and health functional foods, and includes Bacillus megaterium deposited under the accession number KCTC14778BP of the present invention, cultures of the strains, and fermented products of the strains. As far as possible, it is not limited thereto. In addition, it can be prepared by adding the strain, the culture of the strain, and the fermented product of the strain according to the present invention to juice, tea, jelly, juice, etc. prepared as a main component, and pills, powders, granules, tablets, tablets , capsules or liquid formulations.

The health functional food (functional food) is the same term as food for special health use (FoSHU), and is a medicine processed to efficiently display bioregulatory functions in addition to nutritional supply, and has high medical effect. means food. Here, "function (sex)" means to obtain useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions. The food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and ingredients commonly added in the art during the preparation. In addition, the formulation of the food may also be prepared without limitation as long as the formulation is recognized as a food. The composition for food of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that can occur when taking drugs for a long time by using food as a raw material, and has excellent portability. Of the foods can be consumed as supplements to enhance the immune-enhancing effect.

The health food (health food) means a food that has an active health maintenance or promotion effect compared to general food, health supplement food (health supplement food) means a food for the purpose of health supplement. In some cases, the terms health functional food, health food, and health supplement food are used interchangeably.

Specifically, the health functional food is Bacillus megaterium deposited with accession number KCTC14778BP, a culture of the strain, and any one or more of the fermented product of the strain, such as beverages, teas, spices, gum, confectionery, etc. It is food added to food materials or manufactured into capsules, powders, suspensions, etc., which means that it brings specific health effects when ingested. It has the advantage of not having any side effects.

The composition may further include a physiologically acceptable carrier. The type of carrier is not particularly limited, and any carrier commonly used in the art may be used.

In addition, the composition may include additional ingredients that are commonly used in food compositions to improve odor, taste, and visual properties. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, and the like may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), and chrome (Cr) may be included. In addition, amino acids such as lysine, tryptophan, cysteine, and valine may be included.

In addition, the composition contains preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching powder and high bleaching powder, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisole (BHA), butylhydroxy toluene (BHT), etc.), coloring agents (tar colorant, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasonings (MSG sodium glutamate, etc.), sweeteners (dulcin, cyclemate, saccharin, Sodium, etc.), flavorings (vanillin, lactones, etc.), expanding agents (alum, D-potassium hydrogen tartrate, etc.), strengthening agents, emulsifiers, thickeners (thickeners), coating agents, gum base agents, foam inhibitors, solvents, food additives such as improvers (food additives) may be included. The additive may be selected according to the type of food and used in an appropriate amount.

Any one or more of the Bacillus megaterium deposited under the accession number KCTC14778BP, the culture of the strain, and the fermented product of the strain may be added as it is or used together with other food or food ingredients, and may be used in a conventional method can be used appropriately. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, the food composition of the present invention may be added in an amount of 50 parts by weight or less, specifically 20 parts by weight or less, based on the food or beverage when preparing food or beverage. However, when ingested for a long period of time for health and hygiene purposes, the amount below the above range may be included, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

An example of the food composition of the present invention may be used as a health beverage composition, and in this case, as in conventional beverages, various flavoring agents or natural carbohydrates may be included as additional components. The aforementioned natural carbohydrates include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrins and cyclodextrins; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol. Sweeteners include natural sweeteners such as thaumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the health beverage composition includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol or a carbonating agent, and the like. In addition, it may contain fruit flesh for the manufacture of natural fruit juice, fruit juice beverages, or vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Specifically, Bacillus megaterium deposited with accession number KCTC14778BP, a culture of the strain, and at least one of the fermented product of the strain may be included in 0.00001 to 100% by weight based on the total weight of the food composition.

One aspect of the present invention provides a method for preparing a fermented product, comprising fermenting with a Bacillus megaterium strain deposited under Accession No. KCTC14778BP.

The "Bacillus megaterium", "strain", and "fermented product" are as described above.

In the present invention, Bacillus megaterium KCTC14778BP is placed in a medium at 20 ° C to 40 ° C for 1 hour to 200 hours and cultured with shaking, and then the cells are separated by centrifugation, and then treated with ultrasonic waves to disrupt the cells to obtain a fermented product It can be manufactured, but is not limited thereto. The medium used in the present invention may be MRS medium, R2A medium or LBS medium, but the type of medium is not limited as long as the strain can be cultured or fermented.

Since the Bacillus megaterium strain of the present invention, the culture of the strain, or the fermented product of the strain has an excellent skin improvement effect, it is highly utilized as a cosmetic composition, quasi-drug composition, and food composition for skin improvement. Accordingly, various industrial applications such as cosmetics, quasi-drugs, and food can be expected.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are intended to illustrate the present invention by way of example, and the scope of the present invention is not limited to these examples.

Example 1. Isolation of Bacillus (Bacillus megaterium KCTC14778BP) strain

A novel Bacillus megaterium CF1-w2-30 strain was obtained from the plant fermentation broth fermented for one year.

The Bacillus megaterium CF1-w2-30 strain was deposited with the Korea Institute of Bioscience and Biotechnology Biological Resources Center (KCTC), an entrusted institution under the Budapest Treaty, on November 16, 2021, and was given accession number KCTC14778BP. The strain was confirmed to have the 16s rRNA nucleotide sequence of SEQ ID NO: 1.

Example 2. Bacillus (Bacillus megaterium KCTC14778BP) Preparation of fermented product

The Bacillus megaterium KCTC14778BP strain was inoculated into MRS medium and cultured with shaking at 30° C. for 4 days, and the cells were separated by centrifugation. After suspending the cells in purified water, ultrasonic waves were applied to disrupt the cells, and finally, a fermented product of Bacillus megaterium KCTC14778BP was prepared. Specific conditions and configurations are shown in Table 1 below.

Strain Bacillus megaterium KCTC14778BP Sonication Amplitude 40, Process time 40 min, Pulse-ON time 1 S, Plus-OFF time 2 S Concentration of Bacillus megaterium KCTC14778BP culture extract 5%

Example 3. Bacillus (Bacillus megaterium KCTC14778BP) Confirmation of hyaluronic acid synthesis enhancement effect of fermented product

Human keratinocyte HaCaT samples were treated for 72 hours at each concentration. Hyaluronic acid synthesis was compared with the control group (DMEM treatment containing 0% serum) using Quantikine ELISA Hyaluronan from R&D Systems, and the moisturizing ability was quantified.

In order to confirm the effect of enhancing hyaluronic acid synthesis of the fermented product of Bacillus (Bacillus megaterium KCTC14778BP), normal cells and normal cells were treated with retinoic acid, Bacillus megaterium KCTC14778BP fermented product treated, and general Bacillus megaterium KCTC 3007 fermented product treated. Hyaluronic acid synthesis rates were compared (Table 2).

sample Hyaluronic acid synthesis rate (% of control) Normal cells (negative control) 0 Normal cells + Retinoic acid (positive control, 1ppm) 15.4 Bacillus megaterium KCTC14778BP fermented product (1%) 20.1 Normal Bacillus Ferment (1%) 17.1

As shown in the results of Table 2, it was confirmed that the hyaluronic acid synthesis rate increased when normal cells were treated with the fermented product of Bacillus (Bacillus megaterium KCTC14778BP). At this time, the increase rate of the fermented Bacillus megaterium KCTC14778BP was better than that of the normal Bacillus fermented product prepared under the same conditions.

Accordingly, it was found that the fermented product of Bacillus megaterium KCTC14778BP of the present invention exhibits excellent effects such as moisturizing and soothing the skin.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should include all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalent concepts rather than the detailed description above.

Korea Research Institute of Bioscience and Biotechnology KCTC14778BP 20211116

Claims (8)

Bacillus megaterium strain deposited with accession number KCTC14778BP.
A cosmetic composition for moisturizing skin comprising at least one of the strain of claim 1, a culture of the strain, and a fermented product of the strain.
The cosmetic composition according to claim 2, wherein the cosmetic composition has an effect of promoting hyaluronic acid synthesis.
A quasi-drug composition for moisturizing skin comprising at least one of the strain of claim 1, a culture of the strain, and a fermented product of the strain.
The quasi-drug composition according to claim 4, wherein the quasi-drug composition has an effect of promoting hyaluronic acid synthesis.
The strain of claim 1, a culture of the strain, and a food composition for moisturizing the skin comprising any one or more of the fermented product of the strain.
The food composition according to claim 6, wherein the food composition has an effect of promoting hyaluronic acid synthesis.
A method for producing a fermented product comprising the step of fermenting with a Bacillus megaterium strain deposited with accession number KCTC14778BP.