KR20130079147A - Composition for hair growth stimulation or hair loss prevention - Google Patents

Abstract

PURPOSE: A composition for promoting hair growth or preventing alopecia is provided to promote hair growth and to manufacture pharmaceutical products, foods, or cosmetic products. CONSTITUTION: A composition for promoting hair growth or preventing alopecia contains fermentations which are prepared from one or more ingredients selected from the group consisting of Citrus unshiu, Ecklonia cava, and rice bran by lactic acid bacteria. An active ingredient is the fermentations. The lactic acid bacteria are prepared by mixing Raphanus sativus powder, salt, sugar, and water, and naturally culturing. The composition contains soybean powder and green tea powder.

Description

Composition for Hair Growth Stimulation or Hair Loss Prevention}

The present invention relates to a composition for promoting hair growth or preventing hair loss.

In recent years, the hair loss population continues to increase due to environmental pollution, side effects of drug abuse, and social and environmental stress.

Human hair grows and falls through growth, degenerative and resting phases, and hair loss is a symptom caused by a small number of hairs in these growth phases and a large number of hairs in the degenerative or resting phases.

To date, the cause of hair loss has not been clearly identified, but in general, excessive sebum secretion due to poor blood circulation, excessive production of dihydrotestosterone, deterioration of scalp function due to peroxide or bacteria, aging, genetic factors, stress The combination of these factors is known to cause hair loss.

In particular, as it is observed that dihydrotestosterone is maintained at a high level in vivo in a person with hair loss symptoms, overproduction of dihydrotestosterone is attracting attention as a major cause of hair loss.

Dihydrotestosterone is another testosterone produced by the testosterone called testosterone by 5α-reductase.

Representative drugs that inhibit hair loss by inhibiting 5α-reductase activity and inhibiting dihydrotestosterone, propecia (component name: finasteride), developed by Merck, USA, and currently approved by FAD (finasteride)) (US Pat. No. 5,554,573, 57,60046, or Korean Patent No. 375083).

The present invention also discloses a lactic acid bacterium fermentation product of dermis, Ecklonia cava and / or rice bran, which inhibits the activity of 5α-reductase and has an effect of promoting hair growth or preventing hair loss even in actual animal experiments and clinical trials.

An object of the present invention is to provide a composition for promoting hair growth or preventing hair loss.

The specific object of the present invention will be presented below.

As confirmed in the following Examples, Comparative Examples and Experimental Examples, the present inventors fermented the dermis, Ecklonia cava or rice bran with lactic acid bacteria, and 5α-li known to promote hair loss by producing dihydrotestosterone for the fermented product. As a result of examining the inhibitory activity of ductase, all fermented products showed higher inhibitory activity of 5α-reductase than the extract of dermis, Ecklonia cava or rice bran. It was confirmed that the fermented rice bran has a higher activity than the extract of dermis, Ecklonia cava or rice bran.

The present invention is provided on the basis of the experimental results, the composition for promoting hair growth prevention or hair loss of the present invention is characterized in that it comprises a fermented product by the lactic acid bacteria of dermis, Ecklonia cava and / or rice bran as an active ingredient.

In the present specification, "dermis" refers to the peel of Jeju citrus sunki , which is a native of Jeju, and the physiological activity of the dermis has been reported antioxidant activity, antimicrobial activity, anti-inflammatory activity, blood pressure lowering activity (Jeong WS, Park SW, Chung SK.Food Sci.Biotechnol. 6: 292-296 (1997); Kim YC, Koh KS, Koh JS.Food Sci.Biotechnol. 10: 483-487 (2001); Cho C, Park BJ, Chung SH, Kim CB, Cha BS, Byun MW.Food Sci.Biotechnol. 13: 384-386 (2004); Murakami A, et al. Carcinogenesis 21: 1843-1850 (2000)).

In addition, in the present specification, the "rice bran" is a by-product obtained in the process of refining brown rice with white rice, and means that it includes rice snow and a whistle layer. It is to be understood that the rice bran is included in the rice bran as long as it includes the rice hull and the whistle layer, even though it additionally includes the outer skins and / or seedlings of the outer skin of brown rice and the endosperm and / or the endosperm.

In addition, in this specification, "hair loss" should be understood as including all the symptoms classified as hair loss in the art regardless of the direct and indirect cause of the occurrence. Specifically, it includes all of the symptoms of hair loss due to poor blood circulation, excessive sebum secretion due to excessive production of dihydrotestosterone, deterioration of the scalp function by peroxides or bacteria, aging, genetic factors, stress, and their complex actions. .

In addition, in the present specification, the "active ingredient" refers to a component that shows activity alone or in combination with an adjuvant (carrier) having no activity.

Other terms not specifically defined in this specification are intended to refer to either a national language meaning or a commonly accepted meaning in the art.

The lactic acid bacteria that can be used for the preparation of the fermentation product as an active ingredient of the present invention can be used without any particular limitation, specifically, Lactobacillus genus Lactobacillus sp . ), Streptococcus spp. sp . , Pediococcus sp . , Leuconostoc sp . ) And a Bifidobacterium lactic acid bacteria (Bifidobacterium sp . ) Can be used. Preferably, however, the following examples used microorganisms naturally inoculated in a mixture of radish powder and salt, so that the lactic acid bacteria were inoculated into the salted vegetables and fermented so that the lactic acid bacteria were Leuconostoc ( Leuconostoc). kimchii ), Leuconostoc citreum), current Kono Stock mesen's steroid (Leuconostoc mesenteroides), current Kono Stock visible Komi tatum (Leuconostoc gasicomitatum ), Leuconostoc lactis ) and Lactobacillus plantarum, and / or Lactobacillus sakei . Most preferably, as in the following examples, the lactic acid bacteria preparations that are spawn cultured, that is, lactic acid bacteria preparations obtained by mixing salt, sugar and water in a beet powder and culturing them in a natural state. Here, "cultivate in natural state" means a state in which no artificial manipulation is applied in culture conditions such as temperature, humidity, CO ₂ concentration, inoculated microorganism, presence of oxygen, and the like.

Carbon and energy sources of lactic acid bacteria in the dermis, Ecklonia cava and / or rice bran in order to promote fermentation by the lactic acid bacteria before inoculating and fermenting the lactic acid bacteria in the dermis, Ecklonia cava and / or rice bran during the preparation of the fermented product, which is an active ingredient of the present invention. Can be added. As such a carbon source and an energy source, any known in the art can be used, and oligosaccharides, lactose, glucose, fructose, sugar, mixtures thereof, and the like will be used. These carbon sources and energy sources may be added in any range in consideration of the intended fermentation time, fermentation degree and the like. It will be added in the range of 3 to 30 parts by weight based on 100 parts by weight of dermis, Ecklonia cava and / or rice bran.

In addition, the fermentation by lactic acid bacteria for the production of fermented product of the active ingredient of the present invention can be made in the range of 15 ℃ ~ 45 ℃, preferably can be made in the range of 35 ℃ ~ 40 ℃. If the fermentation temperature is lower than the above range, the fermentation rate may be slow, and the desired fermentation product may be produced. If the fermentation temperature is higher than the above range, fermentation rate may be slowed or the desired fermentation product may be produced as the fermentation microorganisms are killed. have.

In addition, a fermentation step may be further included after the fermentation by lactic acid bacteria, which is to stabilize the fermentation products by the microorganisms after the growth of the inoculated microorganisms is stopped, such a fermentation step is performed for 3 days to 12 months Can be.

In addition, fermentation by lactic acid bacteria can be anaerobic or aerobic because it can be cultured under both anaerobic and aerobic conditions.

On the other hand, in the following Examples and Experimental Examples, the mixed fermentation of dermis, Ecklonia cava and rice bran, its 5α-reductase inhibitory activity and hair growth promoting activity in animal experiments is significantly higher than the fermentation products of dermis, Ecklonia cava and rice bran. Therefore, the active ingredient of the present invention is preferably obtained by fermenting a mixture of dermis, Ecklonia cava and rice bran by lactic acid bacteria. In addition, in the following examples, when the green tea powder and soybean powder are added to the mixture and fermented, 5α-reductase inhibitory activity and hair growth promoting activity in animal experiments are further increased. Therefore, the active ingredient of the present invention is more preferably obtained by fermenting a mixture of dermis, Ecklonia cava and rice bran added with green tea powder and soybean powder by lactic acid bacteria.

The fermented product, which is an active ingredient of the present invention, may be in the form of a liquid or powder obtained by concentrating the fermented product itself in a manner such as low temperature drying or freeze drying under reduced pressure. It may be in the form, or may be in the form of a liquid or powder obtained by concentrating and drying the filtrate and the filtered residue. It is also possible to extract the fermented product with an appropriate extraction solvent (for example, water, ethanol or a mixed solvent thereof).

On the other hand, the composition of the present invention may contain any amount (effective amount) of the fermented product as an active ingredient, as long as it can exhibit hair growth promoting activity or hair loss preventing activity according to the properties, uses, formulations, formulation purposes, etc. of the fermentation product. Typical effective amounts will be determined within the range of 0.001% to 15% by weight, based on the total weight of the composition. Specific effective amounts can be determined experimentally within the range of ordinary skill in the art.

The composition of the present invention can be used as a pharmaceutical composition in a specific embodiment.

The pharmaceutical composition of the present invention includes oral formulations (tablets, suspensions, granules, emulsions, capsules, syrups, etc.), parenteral formulations (sterilized), including pharmaceutically acceptable carriers, excipients, etc., in addition to the active ingredients thereof. Aqueous or oily suspensions for injection), topical formulations (solutions, creams, ointments, gels, lotions, patches) and the like.

As used herein, "pharmaceutically acceptable" means that the subject of application (prescription) does not have more toxicity (adequately low toxicity) to which the subject of application (prescription) is adaptable without inhibiting the activity of the active ingredient.

Examples of pharmaceutically acceptable carriers include lactose, glucose, sucrose, starch (such as corn starch, potato starch, etc.), cellulose, derivatives thereof (such as sodium carboxymethyl cellulose, ethylcellulose, etc.) malt, gelatin, talc, solids Lubricants (e.g. stearic acid, magnesium stearate, etc.), calcium sulfate, vegetable oils (e.g. peanut oil, cottonseed oil, sesame oil, olive oil, etc.), polyols (e.g. propylene glycol, glycerin, etc.), alginic acid, emulsifiers (e.g. TWEENS), wetting agents (e.g. Sodium lauryl sulfate), colorants, flavoring agents, tableting agents, stabilizers, antioxidants, preservatives, water, saline, phosphate buffer solutions and the like. The carrier may be selected from one or more of suitable pharmaceutical formulations according to the formulation of the pharmaceutical composition of the present invention.

Excipients may be selected and used according to the formulation of the pharmaceutical composition of the present invention, for example, when the pharmaceutical composition of the present invention is prepared with an aqueous suspending agent, suitable excipients are sodium carboxymethyl cellulose, methyl cellulose, hydropropylmethylcellulose And suspending agents and dispersing agents such as sodium alginate and polyvinylpyrrolidone. Suitable excipients when prepared from injection solutions include Ringer's solution, isotonic sodium chloride, and the like.

The pharmaceutical compositions of the present invention may be administered orally or parenterally, and may optionally be administered topically, as in the case of atopic dermatitis compositions.

The daily dose of the pharmaceutical composition of the present invention is usually 0.001 to 150 mg / kg body weight, and may be administered once or several times. However, since the dosage of the pharmaceutical composition of the present invention is determined in view of various related factors such as route of administration, age, sex, weight, and patient's severity of the patient, the dose is limited in any aspect to the scope of the present invention Should not be understood to be.

The composition of this invention can be grasped | ascertained by food compositions, such as a functional drink, in a specific aspect.

The food composition of the present invention may contain sweetening agents, flavoring agents, physiologically active ingredients, minerals and the like in addition to the active ingredients thereof.

Sweetening agents may be used in an amount that sweetens the food in a suitable manner, and may be natural or synthetic. Preferably, natural sweeteners are used. Examples of natural sweeteners include sugar sweeteners such as corn syrup solids, honey, sucrose, fructose, lactose and maltose.

Flavors may be used to enhance taste or flavor, both natural and synthetic. Preferably, a natural one is used. When using natural ones, the purpose of nutritional fortification can be performed in addition to the flavor. The natural flavor may be obtained from apples, lemons, citrus fruits, grapes, strawberries, peaches, and the like, or may be obtained from green tea leaves, round leaves, jujube leaves, cinnamon, chrysanthemum leaves, jasmine and the like. Also, those obtained from ginseng (red ginseng), bamboo shoots, aloe vera, banks and the like can be used. The natural flavoring agent may be a liquid concentrate or a solid form of extract. Synthetic flavors may be used depending on the case, and synthetic flavors such as esters, alcohols, aldehydes, terpenes and the like may be used.

As the physiologically active substance, catechins such as catechin, epicatechin, gallocatechin, epigallocatechin, vitamins such as retinol, ascorbic acid, tocopherol, calciferol, thiamine, riboflavin, and the like can be used.

As the mineral, calcium, magnesium, chromium, cobalt, copper, fluoride, germanium, iodine, iron, lithium, magnesium, manganese, molybdenum, phosphorus, potassium, selenium, silicon, sodium, sulfur, vanadium and zinc can be used.

In addition, the food composition of the present invention may contain preservatives, emulsifiers, acidifiers, thickeners and the like as needed in addition to the above sweeteners.

Such preservatives, emulsifiers and the like are preferably added in a very small amount as long as they can attain an application to which they are added. The term " trace amount " means, when expressed numerically, in the range of 0.0005% by weight to about 0.5% by weight based on the total weight of the food composition.

Examples of the preservative which can be used include calcium sodium sorbate, sodium sorbate, potassium sorbate, calcium benzoate, sodium benzoate, potassium benzoate and EDTA (ethylenediaminetetraacetic acid).

Examples of the emulsifier which can be used include acacia gum, carboxymethyl cellulose, xanthan gum, pectin and the like.

Examples of the acidulant that can be used include acid, malic acid, fumaric acid, adipic acid, phosphoric acid, gluconic acid, tartaric acid, ascorbic acid, acetic acid, and phosphoric acid. Such an acidulant may be added so that the food composition has a proper acidity for the purpose of inhibiting the growth of microorganisms other than the purpose of enhancing the taste.

Agents that may be used include suspending agents, sedimentation agents, gel formers, bulking agents and the like.

In addition, herbal medicines may be added to enhance flavor and palatability and to add other functionalities (such as preventing arthritis or osteoporosis). Extracts, tortoiseshell extracts, cornus extracts, goji berry extract, licorice extract, donkey extract, brown root extract, kangjinhyang extract, haphwanpi extract, mountain rump extract, lump extract, ginseng extract and the like can be exemplified.

In another aspect, the present invention can be understood as a cosmetic composition.

When the composition of the present invention is conceived as a cosmetic composition, the cosmetic composition can be prepared in various forms, for example, emulsions, lotions, creams (oil-in-water, water-in-oil, multiphase), solutions, suspensions (anhydrous and Aqueous), anhydrous products (oil and glycol based), gels, masks, packs or powders.

The composition of the present invention may contain an acceptable carrier in cosmetic preparations in addition to its active ingredients.

As used herein, the term " acceptable carrier for a cosmetic preparation "refers to a compound or composition which is already known and used in the cosmetic preparation, or which is a compound or composition to be developed in the future, and which is not toxic to the human body.

The carrier may be included in the composition of the present invention in an amount of from about 1% by weight to about 99.99% by weight, preferably from about 50% by weight to about 99% by weight of the composition, based on the total weight thereof.

However, since the ratio depends on the above-mentioned formulation of the cosmetic product and its specific application site (face or hands) or the desired amount of application thereof, the ratio is to limit the scope of the present invention in any aspect It should not be.

Examples of the carrier include alcohols, oils, surfactants, fatty acids, silicone oils, humectants, moisturizers, viscosifiers, emulsifiers, stabilizers, sunscreens, coloring agents and perfumes.

Alcohols, oils, surfactants, fatty acids, silicone oils, wetting agents, humectants, viscosity modifiers, emulsions, stabilizers, sunscreens, colorants, compounds / compositions that can be used as fragrances, etc., which can be used as the carrier are already known in the art. As a result, those skilled in the art can select and use appropriate materials / compositions.

As described above, the present invention can provide a composition for promoting hair growth or preventing hair loss. The composition for promoting hair growth or preventing hair loss of the present invention may be commercialized as a drug, food or cosmetics.

Hereinafter, the present invention will be described with reference to Examples, Comparative Examples, and Experimental Examples. However, the scope of the present invention is not limited to these examples, comparative examples and experimental examples.

< Example > dermis, Moth  Or rice bran Fermented  Produce

<Example 1> dermis fermentation preparation of

3.0 parts by weight of dermis powder was mixed with 100 parts by weight of water, and 1.0% by weight of sugar was added based on 100 parts by weight of sugar as a carbon source and energy source of microorganisms inoculated into the mixture, and 2% (v / v) was inoculated with a culture (about room temperature and aerobic conditions) in about 15 days to prepare a liquid dermal fermentation. Next, the liquid fermentation was sterilized at a sterilization temperature of 121 ° C. for about 20 minutes and concentrated under reduced pressure to obtain a solid fermentation product.

The lactobacillus spawn culture medium is added to 100 parts by weight of radish powder 100 parts by weight based on 100 parts by weight of salt and 400 parts by weight of water and 2.0 parts by weight of sugar which can be a carbon source and energy source of the microorganism by 35 to 50 ℃ Obtained by incubation for 15 days in the temperature range.

Example 2 Eckloniasis Preparation of Fermented Products

Ecklonia cava fermentation product was prepared in the same manner as in <Example 1>, but an Ecklonia cava fermentate was prepared using the same amount of Ecklonia cava powder instead of the dermis powder.

<Example 3> preparation of fermented rice bran

Rice bran fermented product was prepared in the same manner as in <Example 1>, but rice bran fermented product was prepared using the same amount of rice bran powder instead of dermis powder.

Example 4 Dermal, Ecklonia cava and Rice Bran Mixed Fermentation Production Example 1

The fermented product was prepared in the same manner as in <Example 1>, but instead of the dermis powder, the dermis, Ecklonia cava and rice bran mixture were used as 1.0 parts by weight based on 100 parts by weight of water, respectively. Prepared.

Example 5 Dermal, Ecklonia cava and Rice Bran Mixed Fermentation Production Example 2

In the same manner as in <Example 4> to prepare a fermentation product of dermis, Ecklonia cava and rice bran mixture, the fermentation product of dermis, Ecklonia cava and rice bran mixture by adding 0.5 parts by weight of soybean powder and green tea powder, based on 100 parts by weight of water, respectively. Prepared.

< Comparative example > dermis, Moth  Or preparation of rice bran extract

<Comparative Example 1> Preparation of dermis extract

30 parts by weight of dermal powder based on 100 parts by weight of ethanol and 100 parts by weight of 70% ethanol were stirred and extracted at room temperature for 24 hours. The extract was filtered with a 2 μm filter paper, and the filtrate was concentrated under reduced pressure to obtain a solid extract.

Comparative Example 2 Preparation of Ecklonia cava Extract

Ecklonia cava extract was prepared in the same manner as in <Comparative Example 1>, but an equivalent amount of ecstatic powder was used instead of the dermis powder.

Comparative Example 3 Preparation of Rice Bran Extract

Rice bran extract was prepared in the same manner as in <Comparative Example 1>, but the same amount of rice bran powder was used instead of the dermis powder.

< Experimental Example > Experiment to promote hair growth or prevent hair loss

<Experiment 1> 5α- reductase inhibitory activity test

5α-reductase was obtained as a suspension from liver of 8-week-old Sprague-Dawley rats and quantified by Bradford method. To 60 µl of enzyme reaction solution (50 mM Na ₂ HPO ₄ (pH 6.8), 25 mM KCl, 500 mM NADPH) add 30 µl of the 5α-reductase suspension to the sample solution (sample concentration 2.0% (w / v)). 10 µl was added and reacted at 37 ° C for 2 minutes. Thereafter, the reaction solvent was removed by evaporation, and the residue was dissolved in 20 µl of chloroform and developed with a developing solvent (toluene: acetone = 4: 1) for 30 minutes on a thin-layer chromatography plate (TLC), and then the developed plate was hyper The film was sensitized and the amount of testosterone and dihydrotestosterone were quantitatively analyzed by a densitometer.

The activity inhibition rate for 5α-reductase was calculated by the following formula and the results are shown in the following [Table 1].

5α-reductase activity = 100 (A-B / A)

A: Conversion rate of testosterone to dihydrotestosterone when no sample is added

B: Conversion rate of testosterone to dihydrotestosterone when sample lava seawater is added

% Inhibition of activity against 5α-reductase division % Inhibition Example 1 39.6 Example 2 41.9 Example 3 49.6 Example 4 56.8 Example 5 72.5 Comparative Example 1 21.5 Comparative Example 2 14.8 Comparative Example 3 27.6

The results in [Table 1] show that the 5α-reductase inhibitory activity of the dermis, Ecklonia cava and rice bran fermentation is higher than the 5α-reductase inhibitory activity of the extract of dermis, Ecklonia cava and rice bran. In particular, the 5α-reductase inhibitory activity of the dermis, Ecklonia cava and rice bran mixed fermentation of <Example 4> is high, and the 5α-reduct of fermented product <Example 5> obtained by adding soybean and green tea during the fermentation of dermis, Ecklonia cava and rice bran. The highest inhibitory activity was shown.

Experimental Example 2 Animal Experiments for Promoting Hair Growth

Animal experiments for promoting hair growth were performed using male mice C57BL / 6, 7-8 weeks old, and were used for the experiment after adapting to the laboratory environment for 1 week.

Before starting the experiment, the animals were anesthetized with diethyl ether, and the back hairs were firstly removed using a clipper, and the remaining hairs were completely removed using a hair removal cream (Veet, Reckitt Benckiser France). After depilatory cream wiped with ethanol.

Five rats were assigned to each of the experimental group and the control group, and the experimental group was dissolved in 3% (w / v) of physiological saline, and applied 0.3 ml to the back of the epilated area once every two weeks, and only the saline was applied to the control group. . After the experiment and after the experiment, the image obtained by the digital camera was analyzed with an image analyzer (Image analyzer / Image-Pro Plus, USA), and the hair growth efficiency was evaluated by the ratio of the area where the hair was removed to the area where the hair was removed. .

The results are shown as MEAN ± SD in [Table 2] below.

Animal test results for promoting hair growth division after 2 weeks Control group 39.7 ± 7.6 Example 1 54.5 ± 9.3 * Example 2 52.5 ± 11.2 * Example 3 59.2 ± 8.4 * Example 4 66.3 ± 9.2 * Example 5 76.4 ± 12.6 * Comparative Example 1 46.9 ± 6.2 * Comparative Example 2 44.1 ± 9.2 Comparative Example 3 51.5 ± 10.3 * * p <0.05

The results of [Table 2] showed similar trends of the results of [Table 1], and all of the fermentation promoting effect was higher than that of the extract of dermis, Ecklonia cava and rice bran. And the hair growth promoting effect of the fermentation product of <Example 5> was the most excellent.

<Experiment 3> Sensory evaluation experiment for promoting hair growth

In <Experimental Example 1> and <Experimental Example 2> the sensory evaluation for promoting the hair growth of the fermentation product of <Example 5> showed the most excellent hair growth promoting effect was performed as follows.

Specifically, the shampoo is prepared from 2.0% by weight of fermented product of <Example 5>, evening Primrose oil, 15% by weight of sodium hydroxide, 2.0% by weight of TWEEN80, 2.0% by weight of collagen, 1.0% by weight of fragrance, and 0.1% by weight of purified water. The number of users who answered that the hair growth promoting effect was obtained by using the drug once a day for 4 weeks for the users is shown in Table 3 below.

As a shampoo of the comparative example, instead of 2.0% by weight of the fermented product of <Example 5>, a shampoo prepared by further including the amount of purified water was used.

Sensory evaluation results for promoting hair growth division Has hair growth promoting effect No hair growth promoting effect Manufacturing example 14 6 Comparative example 2 18

The results of Table 3 indicate that 14 people, corresponding to 70%, had a hair growth promoting effect when using the shampoo of the preparation prepared by adding the fermentation product of <Example 5>, whereas the fermentation of <Example 5> When using the shampoo of Comparative Example prepared without the addition of water, only two people said that the hair growth promoting effect.

Claims (5)

Hair growth promoting or hair loss prevention composition comprising a fermentation product by at least one lactic acid bacteria selected from the group consisting of dermis, Ecklonia cava and rice bran as an active ingredient.
The method of claim 1,
The active ingredient is a composition for promoting hair growth or preventing hair loss, characterized in that the fermented product by the lactic acid bacteria of the mixture of dermis, Ecklonia cava and rice bran.
The method of claim 1,
The lactic acid bacterium is a composition for promoting hair growth or preventing hair loss, characterized in that obtained by mixing salt and sugar and water in the radish powder and culturing it in a natural state.
The method of claim 2,
A composition for promoting hair growth or preventing hair loss, characterized in that soybean powder and green tea powder are added to the mixture.
5. The method according to any one of claims 1 to 4,
The composition is an atopic dermatitis improving composition, characterized in that the pharmaceutical composition, cosmetic composition or food composition.