KR20230091024A - Novel Lactobacillus fructivorans strain and use thereof - Google Patents

Abstract

The present invention is a Lactobacillus fructivorans strain deposited with accession number KCTC14776BP; And to a cosmetic composition, quasi-drug composition, or food composition comprising at least one selected from the group consisting of the strain, culture of the strain, and fermented product of the strain,
Since the Lactobacillus fructivorans strain, the culture of the strain, or the fermented product of the strain has an excellent skin improvement effect, it is highly utilized as a cosmetic composition, quasi-drug composition, and food composition for skin improvement. Accordingly, various industrial applications such as cosmetics, quasi-drugs, and food can be expected.

Description

Novel Lactobacillus fructivorans strain and use thereof {Novel Lactobacillus fructivorans strain and use thereof}

The present invention is a Lactobacillus fructivorans strain deposited with accession number KCTC14776BP; And to a cosmetic composition, quasi-drug composition, or food composition comprising at least one selected from the group consisting of the strain, culture of the strain, and fermented product of the strain.

Cosmetic ingredients are generally known to cause various skin problems such as inflammation, pimples, and swelling (Maibach. H. I., Contact Dermatitis, 6. 369-404,

1980). In addition, various substances used as raw materials for functional cosmetics are also low on the skin.

There are problems with stability or insignificant effects.

For example, retinoids, adenosine, animal placenta-derived proteins, and chlorella extracts are known as cosmetics for improving skin wrinkles and elasticity. Retinol, which is the most widely known, has limitations in usage due to safety issues such as irritation when applied to the skin, and chlorella extract and the like are insignificant, so it is difficult to expect substantial skin elasticity enhancement and wrinkle improvement effects.

Inflammation occurs as a series of defenses aimed at minimizing the response when cells or tissues are damaged by any cause and restoring the damaged area to its original state. It causes swelling, redness, fever, etc., and causes functional disorders. Causes of inflammation include physical factors such as trauma, frostbite, burns, radiation, chemical factors such as acids, and immunological factors such as antibody reactions. do. Dilation of blood vessels occurs by various chemical mediators secreted by cells damaged by external stimuli, and as permeability increases, antibody, complement, plasma, and phagocytic cells flock to the inflammation site. This phenomenon causes erythema.

An anti-inflammatory agent is an agent that acts to remove the inflammatory source and reduce the physiological response and symptoms in order to disappear inflammation. Substances that have been used for anti-inflammatory purposes so far include flufenamic acid, ibuprofen, benzydamine, and indomethacin as non-steroidal agents, and prednisolone as a steroid type. ), dexamethasone, etc., but most of them have problems in terms of safety for the skin or stability when containing cosmetics, so their use is limited.

Therefore, in recent years, many studies have been conducted on cosmetic compositions that have excellent skin soothing, antioxidant, anti-inflammatory, or moisturizing effects to improve skin conditions (wrinkles, elasticity, etc.) or prevent skin aging or skin damage. It is becoming.

Under this background, the present inventors have conducted research on raw materials that are safer and have excellent skin improvement effects from natural resources, and the new strain of Lactobacillus fructivorans has a skin improvement effect, compared to conventional Lactobacillus fermented products. The present invention was completed by confirming that the skin improvement effect was remarkably excellent.

One object of the present invention is to provide a Lactobacillus fructivorans strain deposited under accession number KCTC14776BP.

Another object of the present invention is to provide a cosmetic composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

Another object of the present invention is to provide a quasi-drug composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

Another object of the present invention is to provide a food composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

Another object of the present invention is to provide a method for producing a fermented product, comprising the step of fermenting with a Lactobacillus fructivorans strain deposited under Accession No. KCTC14776BP.

Hereinafter, the contents of the present invention will be described in detail. On the other hand, the description and embodiment of one aspect disclosed in the present invention can also be applied to the description and embodiment of another aspect with respect to common matters. Additionally, all combinations of the various elements disclosed herein fall within the scope of the present invention. In addition, it cannot be seen that the scope of the present invention is limited by the specific descriptions described below.

One aspect of the present invention provides a Lactobacillus fructivorans strain deposited under accession number KCTC14776BP.

The term of the present invention, “Lactobacillus fructivorans” is a benign bacterium and refers to one species of Lactobacillus (lactic acid bacteria) belonging to the genus Fructilactobacillus. This species is found in wine, beer, grape must, dairy products, sauerkraut, meat and fish. The characteristics of Lactobacillus fructivorans vary depending on the growth region and natural environmental conditions, so the activity of the strain and its fermented product may vary, and the Lactobacillus fructivorans of the present invention may be derived from a fermentation broth, but is not limited thereto. .

In the present invention, the fermentation broth may be fermented for 6 months to 2 years, specifically for 9 months to 1 year and 6 months, and more specifically for 1 year to prepare a fermentation broth, and the fermentation broth may be a plant fermentation broth, but is not limited thereto.

In one embodiment of the present invention, a fermented liquid was prepared, and it was confirmed that the fermented product of Lactobacillus fructivorans of the present invention had an excellent skin improvement effect compared to the fermented product of general Lactobacillus.

Another aspect of the present invention provides a cosmetic composition for improving skin comprising any one or more of the strain, culture of the strain, and fermented product of the strain.

As used herein, the term “strain” refers to a collection of individuals derived from a single cell, proliferating through asexual reproduction and having homogeneous genetic characteristics, and within one species, genetic characteristics Several different strains (genetic variants) exist. In the present invention, the strain refers to Lactobacillus fructivorans, and may specifically be Lactobacillus fructivorans deposited under accession number KCTC14776BP, but is not limited thereto.

In the present invention, the strain may include cells of the Lactobacillus fructivorans strain, dried cells, lysates, and the like. At this time, the dried cells may be spray-dried bacteria, freeze-dried bacteria, vacuum-dried bacteria, drum-dried bacteria, etc., and the lysate is a product obtained by breaking the cell walls of the strain itself by chemical or physical force. can be

The "culture" of the present invention means a product obtained by culturing the strain in a medium. For example, the culture of the present invention may include components remaining in the medium remaining after harvesting the strain from the culture medium of Lactobacillus fructivorans.

The culture may be a whole culture, a culture supernatant, a lysate, a fraction thereof, or the like of the Lactobacillus fructivorans strain. At this time, the culture supernatant can be obtained by centrifuging the culture of the strain, the lysate can be obtained by physically or sonicating the strain, and the fraction is the culture, culture supernatant, lysate, etc. It can be obtained by applying methods such as centrifugation and chromatography.

Any medium and other culture conditions used for culturing the strain of the present invention may be used without particular limitation as long as it is a medium used for the culture of conventional microorganisms of the genus Lactobacillus. It can be cultured while controlling temperature, pH, etc. under aerobic or anaerobic conditions in a normal medium containing phosphorus, inorganic compounds, amino acids, and/or vitamins.

Examples of the carbon source in the present invention include carbohydrates such as glucose, fructose, sucrose, and maltose; sugar alcohols such as mannitol and sorbitol; organic acids such as pyruvic acid, lactic acid, citric acid and the like; Amino acids such as glutamate, methionine, lysine, and the like may be included, but are not limited thereto. In addition, natural organic nutritional sources such as starch hydrolysate, molasses, blackstrap molasses, rice winter, cassava, sorghum and corn steep liquor can be used, as well as glucose and pasteurized pretreated molasses (i.e., molasses converted to reducing sugars). Carbohydrates such as may be used, and various other carbon sources in appropriate amounts may be used without limitation. These carbon sources may be used alone or in combination of two or more.

Examples of the nitrogen source include inorganic nitrogen sources such as ammonia, ammonium sulfate, ammonium chloride, ammonium acetate, ammonium phosphate, ammonium carbonate, and ammonium nitrate; Organic nitrogen sources such as amino acids, peptones, NZ-amines, meat extracts, yeast extracts, malt extracts, corn steep liquor, casein hydrolysates, fish or degradation products thereof, defatted soybean cakes or degradation products thereof, and the like can be used. These nitrogen sources may be used alone or in combination of two or more, but are not limited thereto.

The number of persons may include monopotassium phosphate, dipotassium phosphate, or a sodium-containing salt corresponding thereto. As the inorganic compound, sodium chloride, calcium chloride, iron chloride, magnesium sulfate, iron sulfate, manganese sulfate, calcium carbonate, and the like may be used.

In addition, amino acids, vitamins, and/or appropriate precursors may be included in the medium. Specifically, L-amino acids and the like may be added to the culture medium of the strain. Specifically, glycine, glutamate, and/or cysteine may be added, and if necessary, L-amino acids such as lysine may be further added, but it is not necessarily limited thereto. don't

The medium or precursor may be added to the culture in a batch or continuous manner, but is not limited thereto.

In the present invention, the pH of the culture can be adjusted by adding compounds such as ammonium hydroxide, potassium hydroxide, ammonia, phosphoric acid, sulfuric acid and the like to the culture in an appropriate manner during cultivation of the strain. In addition, during cultivation, the formation of bubbles can be suppressed by using an antifoaming agent such as a fatty acid polyglycol ester. In addition, in order to maintain the aerobic state of the culture, oxygen or oxygen-containing gas may be injected into the culture, or nitrogen, hydrogen or carbon dioxide gas may be injected without injection of gas or gas to maintain the anaerobic and non-aerobic state.

The temperature of the culture may be 25 °C to 40 °C, more specifically 28 °C to 37 °C, but is not limited thereto. The culturing period may be continued until a desired production amount of a useful substance is obtained, and specifically may be 1 hour to 200 hours, but is not limited thereto.

In the present invention, the term "fermentation" means that microorganisms are not decay reactions during the process of decomposing organic matter using their own enzymes. Fermentation and decay reactions proceed by similar processes, but when useful substances are produced as a result of decomposition, it is called fermentation, and when bad smells or harmful substances are produced, it is called decay.

In the present invention, the method for obtaining the fermented product from the strain is not particularly limited, but the strain is inoculated into a medium, shaken incubated at 20 ° C to 40 ° C for 1 hour to 200 hours, and then the cells are separated by centrifugation, A fermented product can be obtained by a method of disrupting the cells by ultrasonication. Alternatively, it can be obtained according to a method commonly used in the art or similar fields.

In the present invention, the type of medium in which the strain is inoculated is not limited as long as the strain can proceed with fermentation.

In the present invention, the fermentation product obtained from the strain is not only the fermented material itself, but also the culture medium of the strain in which the strain and the culture coexist, the fermentation product obtained by filtering the strain from the culture medium, and the strain from the culture medium. A fermented product obtained by filtering it, an extract obtained by extracting the fermented product or a culture medium containing the same, a diluted solution obtained by diluting the fermented product or its extract, a dried product obtained by drying the fermented product or its extract, and a cell body of the strain collected and crushed Lysates, etc., include all kinds of materials including fermented products generated from the above strains.

The term of the present invention, "skin improvement" may be at least one selected from the group consisting of wrinkle improvement, elasticity improvement, skin soothing, skin inflammation improvement, anti-aging, and skin moisturizing, but is not limited thereto.

As used herein, the term "improvement" refers to any activity that at least reduces a parameter associated with alleviation or treatment of a condition, for example, the degree of symptoms, and includes wrinkle improvement, elasticity improvement, skin soothing, skin inflammation improvement, anti-aging and Any one or more of skin moisturizing may be included.

The skin improvement may be achieved by promoting collagen synthesis, inhibiting NO production, or promoting hyaluronic acid synthesis, but is not limited thereto.

Specifically, the present inventors confirmed that the fermented product of Lactobacillus fructivorans KCTC14776BP has an excellent skin improvement effect compared to the fermented product of general Lactobacillus. More specifically, it was confirmed that the fermented product of Lactobacillus fructivorans KCTC14776BP was excellent in enhancing collagen synthesis, inhibiting NO production, and promoting hyaluronic acid synthesis. Accordingly, it was confirmed that the Lactobacillus fructivorans or fermented product of the present invention is superior in skin improvement effects such as wrinkle improvement, elasticity improvement, skin soothing, skin inflammation improvement and skin moisturizing, compared to general Lactobacillus or its fermented product.

The term "wrinkles" of the present invention can be largely divided into expression wrinkles caused by expression muscles used to make facial expressions and fine wrinkles caused by general or local skin decline. Specifically, wrinkles may be caused by genes, reduction of collagen and elastic fibers present in the skin dermis, external environment, and the like. For the purpose of the present invention, wrinkles may be used as a meaning including both facial expression wrinkles and fine wrinkles, but is not limited thereto.

The term of the present invention, "elasticity" is a force that holds the skin tight when pressed with a finger, and "elasticity improvement" or "elasticity enhancement" means to increase the elasticity of the skin by strengthening the subcutaneous fat layer structure of the subject, It may mean increasing the elasticity of the skin by using the composition of the invention.

The term of the present invention, "aging" refers to a phenomenon in which biological structures and functions decline with the passage of time, and the anti-aging of the present invention is not limited to the extent of aging, such as preventing, suppressing, or delaying aging. don't Wrinkle improvement and anti-aging in the present invention may be due to the promotion of collagen synthesis, but is not limited thereto.

The term of the present invention, "collagen" is a fibrous protein found in most animals, especially mammals, and occupies most of all connective tissues in the body, such as skin and cartilage, and fibroblasts, which are the most common cells in the body. (fibroblasts) produce and secrete collagen. Gelatine, which is widely used in cooking or in the food and pharmaceutical industries, is an irreversible hydrolysis of collagen. Since the decrease in collagen causes wrinkles and is the main cause of reducing skin elasticity, synthesis of collagen is essential for improving wrinkles and enhancing elasticity.

In one embodiment of the present invention, it was confirmed that the fermented product of Lactobacillus fructivorans, which is the Lactobacillus of the present invention, has an excellent collagen synthesis enhancement rate. confirmed (Table 2).

As used herein, “skin soothing” refers to relieving and calming erythema or irritated skin areas, and may include skin irritation relief. Examples include, but are not limited to, reducing skin water loss and/or reducing redness.

As used herein, “inflammation” is a kind of in vivo response to damage or infection of a specific tissue, and the main mediator is immune cells. The purpose of such inflammation is to suppress tissue damage as much as possible, remove infectious agents, and regenerate tissue. In the present invention, skin soothing and regeneration may be due to inhibition of NO production, but is not limited thereto.

The term "NO (nitric oxide, nitrous acid)" of the present invention plays various roles such as removing bacteria and tumors, regulating blood pressure, or mediating nerve transmission, but when an inflammatory reaction occurs, iNOS (inducible nitric oxide) synthase) is increased to produce a large amount of NO, and the excessively produced NO causes tissue damage, gene mutation, nerve damage, etc., and increases vascular permeability to promote inflammatory reactions such as edema. While NO production is increased in inflamed cells, NO production is suppressed when the skin is calmed, so there is an essential correlation between them.

In one embodiment of the present invention, the fermented yeast (Lactobacillus fructivorans KCTC14776BP) of the present invention was superior in the ability to inhibit NO production compared to the fermented yeast, thereby confirming the effect of soothing the skin and improving inflammation (Table 3).

As used herein, the term "moisturizing the skin" refers to all actions that maintain a smooth surface by supplying moisture to the skin or blocking evaporation of moisture to maintain the flexibility of the skin and inducing uniform exfoliation of dead skin cells, and "drying" means a condition in which such skin moisturizing is not sufficient. The skin moisturizing effect can help improve wrinkles and increase elasticity of the skin. Specifically, skin moisturizing may be due to promotion of hyaluronic acid production, but is not limited thereto.

As used herein, the term "hyaluronic acid" is a type of glycosaminoglycans, and refers to a chain-like polymeric polysaccharide material in which glucuronic acid and N-acetylglucosamine residues are repeatedly linked. Hyaluronic acid can contain water equivalent to 300 to 1000 times its own weight, and exhibits a moisturizing effect on the skin by combining with moisture.

In one embodiment of the present invention, it was confirmed that the fermented product of Lactobacillus fructivorans, the lactobacillus of the present invention, had an excellent hyaluronic acid synthesis enhancement rate, and through this, the fermented Lactobacillus of the present invention was confirmed to have a remarkable effect on skin moisturizing, etc. (Table 4).

As used herein, the term “cosmetic composition” refers to a solution, an external ointment, a cream, a foam, a nourishing lotion, a softening lotion, a perfume, a pack, a softening water, an emulsion, a makeup base, an essence, a soap, a liquid cleanser, a bath additive, a sunscreen cream, It may be a formulation selected from the group consisting of sun oil, suspension, emulsion, paste, gel, lotion, powder, soap, surfactant-containing cleansing, oil, powder foundation, emulsion foundation, wax foundation, patch and spray. , but not limited thereto.

The cosmetic composition of the present invention may further include one or more cosmetically acceptable carriers formulated in general skin cosmetics, and as typical ingredients, for example, oil, water, surfactants, moisturizers, lower alcohols, thickeners, A chelating agent, a colorant, a preservative, a flavoring agent, and the like may be suitably blended, but is not limited thereto.

Cosmetically acceptable carriers included in the cosmetic composition of the present invention vary according to the formulation of the cosmetic composition.

When the cosmetic formulation of the present invention is an ointment, paste, cream, or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanthate, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, oxide as a carrier component Zinc or the like may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizing agent or an emulsifying agent may be used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, Propylene glycol, 1,3-butyl glycol oil and the like may be used, and in particular, cottonseed oil, peanut oil, corn germ oil, olive oil, castor oil and sesame oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a suspension, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, Crystalline cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolyzates, isethionates, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugars, etc. may be used as carrier components. It may be, but is not limited thereto. These may be used alone or in combination of two or more.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, polyamide powder or a mixture thereof may be used as a carrier component, and in particular, in the case of a spray, chloro propellants such as fluorohydrocarbons, propane/butane or dimethyl ether.

On the other hand, all of the ingredients described in the present invention may preferably be included in the composition of the present invention within the range of not exceeding the maximum value specified in the regulations on cosmetic safety standards and China's 'cosmetic safety and technical regulations'.

Another aspect of the present invention provides a quasi-drug composition for skin improvement comprising at least one of the strain, culture of the strain, and fermented product of the strain.

“Lactobacillus fructivorans”, “strain”, “culture”, “fermented product”, “skin improvement”, “improvement”, “wrinkles”, “elasticity”, “collagen”, “skin soothing”, “ Inflammation”, “NO”, “skin moisturizing”, and “hyaluronic acid” are as described above.

The term "quasi-drugs" of the present invention refers to body cleansers, disinfectant cleaners, detergents, kitchen cleaners, cleaning cleaners, toothpaste, gargles, wet wipes, detergents, soaps, hand washes, hair cleaners, hair softeners, humidifier fillers, masks, ointments, and filters It may be selected from the group consisting of fillers, but is not limited thereto.

The quasi-drug composition of the present invention may further include a pharmaceutically acceptable carrier, excipient or diluent as needed in addition to the above components. The pharmaceutically acceptable carrier, excipient or diluent is not limited as long as the effect of the present invention is not impaired, and examples thereof include fillers, extenders, binders, wetting agents, disintegrants, surfactants, lubricants, sweeteners, aromatics, preservatives, and the like. can include

Representative examples of the pharmaceutically acceptable carrier, excipient or diluent of the present invention include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, maltitol, starch, gelatin, glycerin, gum acacia, alginate, calcium phosphate, calcium Carbonate, calcium silicate, cellulose, methyl cellulose, microcrystalline cellulose, polyvinyl pyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, mineral oil, propylene glycol, polyethylene glycol, vegetable oil , injectable esters, Witepsol, Macrogol, Tween 61, cacao butter, laurage, and the like.

When the culture of the strain of the present invention or its fermented product is used as a quasi-drug, it may additionally contain one or more active ingredients exhibiting the same or similar functions. For example, it may include known skin beneficial bacteria enhancement, skin harmful bacteria suppression, skin soothing, skin inflammation improvement, skin whitening, skin regeneration, and wound healing components. When an additional wrinkle improvement, skin whitening, skin trouble improvement, skin moisturizing component is included, the wrinkle improvement, elasticity improvement, anti-aging, skin soothing, skin inflammation improvement and skin moisturizing effect of the composition of the present invention can be further increased. When adding the above components, skin safety according to combined use, ease of formulation, and stability of active ingredients may be considered.

The quasi-drug composition of the present invention is a skin anti-aging component known in the art, retinoic acid, TGF, protein derived from animal placenta, betulinic acid and chlorella extract; As anti-inflammatory components known in the art, non-steroidal flufenamic acid, ibuprofen, benzidamine, indomethacin, prednisolone, dexamethasone, allantoin, azene, hydrocortisone; And it may further include one or two or more components selected from the group consisting of derivatives thereof and various plant extracts. Additional components may be included in an amount of 0.0001% to 10% by weight based on the total weight of the composition, and the content range depends on requirements such as skin safety, ease of formulation of the strain, culture or fermented product of the present invention. will be adjustable.

Formulation method, dosage, usage method, components, etc. of quasi-drugs can be appropriately selected from conventional techniques known in the art.

Another aspect of the present invention provides a food composition for improving skin comprising any one or more of the strain, the culture of the strain, and the fermented product of the strain.

“Lactobacillus fructivorans”, “strain”, “culture”, “fermented product”, “skin improvement”, “improvement”, “wrinkles”, “elasticity”, “collagen”, “skin soothing”, “ Inflammation”, “NO”, “skin moisturizing”, and “hyaluronic acid” are as described above.

The term "food" of the invention means meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gum, dairy products including ice cream, various soups, beverages, tea, drinks, alcoholic beverages, vitamins It includes all foods in the conventional meaning of complexes and health functional foods, including Lactobacillus fructivorans deposited under the accession number KCTC14776BP of the present invention, cultures of the strains, and fermented products of the strains. As far as possible, it is not limited to this. In addition, it can be prepared by adding the strain, the culture of the strain, and the fermented product of the strain according to the present invention to juice, tea, jelly, juice, etc. prepared as a main component, and pills, powders, granules, tablets, tablets , capsules or liquid formulations. The health functional food (functional food) is the same term as food for special health use (FoSHU), and is a medicine processed to efficiently display bioregulatory functions in addition to nutritional supply, and has high medical effect. means food. Here, "function (sex)" means to obtain useful effects for health purposes such as regulating nutrients for the structure and function of the human body or physiological functions. The food of the present invention can be prepared by a method commonly used in the art, and can be prepared by adding raw materials and ingredients commonly added in the art during the preparation. In addition, the formulation of the food may also be prepared without limitation as long as the formulation is recognized as a food. The composition for food of the present invention can be prepared in various types of formulations, and unlike general drugs, it has the advantage of not having side effects that can occur when taking drugs for a long time by using food as a raw material, and has excellent portability. Of the foods can be consumed as supplements to enhance the immune-enhancing effect.

The health food (health food) means a food that has an active health maintenance or promotion effect compared to general food, health supplement food (health supplement food) means a food for the purpose of health supplement. In some cases, the terms health functional food, health food, and health supplement food are used interchangeably.

Specifically, the health functional food is a beverage, tea, spice, gum, confectionery, etc. It is a food added to food ingredients or made into capsules, powders, suspensions, etc., which means that when ingested, it brings a specific effect on health, but unlike general medicines, when taking medicines for a long time It has the advantage of not having any side effects that may occur.

The composition may further include a physiologically acceptable carrier. The type of carrier is not particularly limited, and any carrier commonly used in the art may be used.

In addition, the composition may include additional ingredients that are commonly used in food compositions to improve odor, taste, and visual properties. For example, vitamins A, C, D, E, B1, B2, B6, B12, niacin, biotin, folate, panthotenic acid, and the like may be included. In addition, minerals such as zinc (Zn), iron (Fe), calcium (Ca), chromium (Cr), magnesium (Mg), manganese (Mn), copper (Cu), and chrome (Cr) may be included. In addition, amino acids such as lysine, tryptophan, cysteine, and valine may be included.

In addition, the composition contains preservatives (potassium sorbate, sodium benzoate, salicylic acid, sodium dehydroacetate, etc.), disinfectants (bleaching powder and high bleaching powder, sodium hypochlorite, etc.), antioxidants (butylhydroxyanisole (BHA), butylhydroxy toluene (BHT), etc.), coloring agents (tar colorant, etc.), coloring agents (sodium nitrite, sodium nitrite, etc.), bleach (sodium sulfite), seasonings (MSG sodium glutamate, etc.), sweeteners (dulcin, cyclemate, saccharin, Sodium, etc.), flavorings (vanillin, lactones, etc.), expanding agents (alum, D-potassium hydrogen tartrate, etc.), strengthening agents, emulsifiers, thickeners (thickeners), coating agents, gum base agents, foam inhibitors, solvents, food additives such as improvers (food additives) may be included. The additive may be selected according to the type of food and used in an appropriate amount.

Any one or more of Lactobacillus fructivorans deposited under the accession number KCTC14776BP, the culture of the strain, and the fermented product of the strain may be added as it is or used together with other food or food ingredients, It can be used appropriately depending on the method. The mixing amount of the active ingredient may be appropriately determined depending on the purpose of use (prevention, health or therapeutic treatment). In general, the food composition of the present invention may be added in an amount of 50 parts by weight or less, specifically 20 parts by weight or less, based on the food or beverage when preparing food or beverage. However, when ingested for a long period of time for health and hygiene purposes, the amount below the above range may be included, and since there is no problem in terms of safety, the active ingredient may be used in an amount above the above range.

An example of the food composition of the present invention may be used as a health beverage composition, and in this case, as in conventional beverages, various flavoring agents or natural carbohydrates may be included as additional components. The aforementioned natural carbohydrates include monosaccharides such as glucose and fructose; disaccharides such as maltose and sucrose; polysaccharides such as dextrins and cyclodextrins; It may be a sugar alcohol such as xylitol, sorbitol, or erythritol. Sweeteners include natural sweeteners such as thaumatin and stevia extract; Synthetic sweeteners such as saccharin and aspartame may be used. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, specifically about 0.02 to 0.03 g per 100 mL of the composition of the present invention.

In addition to the above, the health beverage composition includes various nutrients, vitamins, electrolytes, flavors, colorants, pectic acid, salts of pectic acid, alginic acid, salts of alginic acid, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, It may contain alcohol or a carbonating agent, and the like. In addition, it may contain fruit flesh for the manufacture of natural fruit juice, fruit juice beverages, or vegetable beverages. These components may be used independently or in combination. The ratio of these additives is not very important, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.

Specifically, Lactobacillus fructivorans deposited under Accession No. KCTC14776BP, a culture of the strain, and a fermented product of the strain may contain 0.00001 to 100% by weight relative to the total weight of the food composition. .

Another aspect of the present invention provides a method for producing a fermented product comprising the step of treating a Lactobacillus fructivorans strain deposited under accession number KCTC14776BP.

The “Lactobacillus fructivorans”, “strain”, and “fermented product” are as described above.

In the present invention, Lactobacillus fructivorans KCTC14776BP is placed in a culture medium at 20 ° C to 40 ° C for 1 hour to 200 hours and cultured with shaking, and then the cells are separated by centrifugation, followed by ultrasonic treatment to break the cells to prepare a fermented product It can, but is not limited thereto. The medium used in the present invention may be MRS medium, R2A medium or LBS medium, but the type of medium is not limited as long as the strain can be cultured or fermented.

Since the Lactobacillus fructivorans strain, the culture of the strain, or the fermented product of the strain of the present invention has an excellent skin improvement effect, it is highly applicable to cosmetic compositions, quasi-drug compositions, and food compositions for skin improvement. Accordingly, various industrial applications such as cosmetics, quasi-drugs, and food can be expected.

Hereinafter, the present invention will be described in more detail through examples. However, these examples are intended to illustrate the present invention by way of example, and the scope of the present invention is not limited to these examples.

Example 1. Isolation of Lactobacillus (Lactobacillus fructivorans KCTC14776BP) strain

A novel lactobacillus strain, Lactobacillus fructivorans CF2-2w-20, was obtained from the plant fermentation broth fermented for one year.

The Lactobacillus fructivorans CF2-2w-20 strain was deposited with the Korea Institute of Bioscience and Biotechnology (KCTC), an entrusted institution under the Budapest Treaty, on November 16, 2021, and was given accession number KCTC14776BP. The strain was confirmed to have the 16s rRNA nucleotide sequence of SEQ ID NO: 1.

Example 2. Preparation of fermented product of Lactobacillus (Lactobacillus fructivorans KCTC14776BP)

The Lactobacillus fructivorans KCTC14776BP strain was inoculated into the medium, and the cells were separated by centrifugation after shaking culture. After suspending the cells in purified water, ultrasonic waves were applied to disrupt the cells, and finally, a fermented product of Lactobacillus fructivorans KCTC14776BP was prepared. Specific conditions and configurations are shown in Table 1 below.

Strain Lactobacillus fructivorans KCTC14776BP Sonication Amplitude 40, Process time 40 min, Pulse-ON time 1 S, Plus-OFF time 2 S Concentration of Lactobacillus fructivorans KCTC14776BP culture extract 5%

Example 3. Lactobacillus (Lactobacillus fructivorans KCTC14776BP) fermented product confirmation of collagen synthesis enhancement effect

Human dermal fibroblast samples were treated for 48 hours at each concentration. The collagen synthesis rate was measured using the procollagen type IC-peptide (PIP) EIA kit method. The collagen synthesis efficacy of the sample was calculated and measured in comparison with the negative control group (DMEM containing 0% serum).

In order to confirm the collagen synthesis enhancement effect of Lactobacillus fructivorans KCTC14776BP fermentation, TGF-β treatment on normal cells and normal cells, Lactobacillus fructivorans KCTC14776BP fermentation product treatment, general Lactobacillus plantarum KCTC 3108 fermentation product treatment Collagen synthesis rates were compared (Table 2).

sample Collagen synthesis rate (% of control) Normal cells (negative control) 0 Normal cells + TGF-β (positive control, 10 ppb) 24.6 Lactobacillus fructivorans KCTC14776BP fermented product (0.1%) 21.77 Normal Lactobacillus fermented product (0.1%) 15.12

As shown in the results of Table 2, it was confirmed that the collagen synthesis rate increased when normal cells were treated with Lactobacillus fructivorans KCTC14776BP fermented product. At this time, the increase rate of the fermented Lactobacillus fructivorans KCTC14776BP was better than that of the normal Lactobacillus fermented product prepared under the same conditions.

Accordingly, it was found that the fermented product of Lactobacillus fructivorans KCTC14776BP of the present invention exhibits excellent effects such as skin wrinkle improvement and elasticity enhancement.

Example 4. Lactobacillus (Lactobacillus fructivorans KCTC14776BP) fermented skin soothing and inflammation improvement effects (NO production inhibition) confirmed

Raw 264.7 cells were diluted for each concentration, treated with 1 μg/ml of LPS, and cultured for 24 hours. The NO production inhibitory ability was evaluated as follows using the NO quantification kit method.

NO production inhibition ability (%) = (1-(NO production amount when sample is added / NO production amount when sample is not added) x 100

sample NO production inhibition ability (% of control) Normal cells (negative control) 0 Normal cells + L-NMMA (positive control, 20ug/mL) 47.9 Lactobacillus fructivorans KCTC14776BP fermented product (1%) 15.1 Generic Lactobacillus Fermentation (1%) 4.9

As shown in the results of Table 3, it was confirmed that the NO production inhibition ability increased when Lactobacillus fructivorans KCTC14776BP fermented product was treated with normal cells. At this time, the increase rate of the fermented Lactobacillus fructivorans KCTC14776BP was better than that of the normal Lactobacillus fermented product prepared under the same conditions.

Accordingly, it was found that the fermented product of Lactobacillus fructivorans KCTC14776BP of the present invention exhibits excellent effects such as skin soothing and inflammation improvement.

Example 5. Lactobacillus (Lactobacillus fructivorans KCTC14776BP) fermented product confirms skin moisturizing effect (hyaluronic acid synthesis enhancement)

Human keratinocyte HaCaT samples were treated for 72 hours at each concentration. Hyaluronic acid synthesis was compared with the control group (DMEM treatment containing 0% serum) using Quantikine ELISA Hyaluronan from R&D Systems, and the moisturizing ability was quantified.

sample Hyaluronic acid synthesis rate (% of control) Normal cells (negative control) 0 Normal cells + Retinoic acid (positive control, 1ppm) 27.6 Lactobacillus fructivorans KCTC14776BP fermented product (0.1%) 47.0 Lactobacillus fructivorans KCTC14776BP fermented product (1%) 86.6 Normal Lactobacillus fermented product (0.1%) 9.6 Normal Lactobacillus Fermentation (1%) 39.2

As shown in the results of Table 4, it was confirmed that the hyaluronic acid synthesis rate increased when normal cells were treated with Lactobacillus fructivorans KCTC14776BP fermented product. At this time, the increase rate of the fermented Lactobacillus fructivorans KCTC14776BP was better than that of the normal Lactobacillus fermented product prepared under the same conditions.

Accordingly, it was found that the fermented product of Lactobacillus fructivorans KCTC14776BP of the present invention exhibits excellent effects such as skin moisturizing.

From the above description, those skilled in the art to which the present invention pertains will be able to understand that the present invention may be embodied in other specific forms without changing its technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and not limiting. The scope of the present invention should include all changes or modifications derived from the meaning and scope of the claims to be described later and their equivalent concepts rather than the detailed description above.

Korea Research Institute of Bioscience and Biotechnology KCTC14776BP 20211116

Claims (11)

Lactobacillus fructivorans strain deposited under accession number KCTC14776BP.
A cosmetic composition for skin improvement comprising at least one of the strain of claim 1, a culture of the strain, and a fermented product of the strain.
The cosmetic composition according to claim 2, wherein the skin improvement is at least one selected from the group consisting of wrinkle improvement, elasticity improvement, anti-aging, skin soothing, skin inflammation improvement, and skin moisturizing.
The cosmetic composition according to claim 2, wherein the cosmetic composition has an effect of promoting collagen synthesis, inhibiting NO production, or promoting hyaluronic acid synthesis.
A quasi-drug composition for skin improvement comprising at least one of the strain of claim 1, a culture of the strain, and a fermented product of the strain.
The quasi-drug composition according to claim 5, wherein the skin improvement is at least one selected from the group consisting of wrinkle improvement, elasticity improvement, anti-aging, skin soothing, skin inflammation improvement, and skin moisturizing.
The quasi-drug composition according to claim 5, wherein the quasi-drug composition has an effect of promoting collagen synthesis, inhibiting NO production, or promoting hyaluronic acid synthesis.
A food composition for skin improvement comprising at least one of the strain of claim 1, a culture of the strain, and a fermented product of the strain.
The food composition according to claim 8, wherein the skin improvement is at least one selected from the group consisting of wrinkle improvement, elasticity improvement, anti-aging, skin soothing, skin inflammation improvement and skin moisturizing.
The food composition according to claim 8, wherein the food composition has an effect of promoting collagen synthesis, inhibiting NO production or promoting hyaluronic acid synthesis.
A method for producing a fermented product comprising the step of fermenting with a Lactobacillus fructivorans strain deposited under accession number KCTC14776BP.