KR102311725B1 - Composition comprising culture or its extract of lactobacillus crustorum - Google Patents

Abstract

Disclosed herein is a composition comprising a Lactobacillus crustorum strain, a lysate thereof, a culture thereof, or an extract of the strain, lysate or culture. In addition, the present specification discloses a Lactobacillus crustorum JTA strain having an accession number of KCCM12048P having at least one skin condition improvement effect among skin moisturizing, skin texture improvement, skin regeneration, skin barrier strengthening and skin elasticity strengthening. .

Description

A composition comprising a culture of Lactobacillus crustorum or an extract thereof {COMPOSITION COMPRISING CULTURE OR ITS EXTRACT OF LACTOBACILLUS CRUSTORUM}

Disclosed herein is a composition comprising a Lactobacillus crustorum strain, a lysate thereof, a culture thereof, or an extract of the strain, lysate or culture.

The surface of the human skin consists of keratinocytes that are differentiated from the basal layer and mature keratinocytes are degenerated. These keratinocytes are bound by the double-chain lamellar structure of ceramides present in large amounts in the stratum corneum, thereby maintaining smooth and elastic skin.

Ceramide is a type of sphingolipid having a structure in which fatty acids are linked to sphingosine or phytosphingosine. It acts as an essential ingredient to show the function, making the stratum corneum firm and flexible.

These ceramides contribute to the maintenance of the skin barrier, the role of the lipid barrier to inhibit the evaporation of moisture, and homeostasis, and provide rapid recovery against external physical and chemical damage. Therefore, if the ceramide is insufficient, the skin becomes dry and damaged, loses elasticity, and the skin becomes rough. In addition, ceramides are decomposed into phytosphingosine on the skin surface, acting as an antibacterial barrier against external harmful microorganisms, and performing important functions in inflammation control and wound healing. Recently, as the role of ceramide-related metabolites as signal transmitters in regulating cell metabolism is known, various studies are being conducted for the purpose of regulating skin physiology. this is being reported

On the other hand, skin regeneration is a tissue response to damage and is a process of tissue recovery, including chemotaxis, cell differentiation and replication, matrix protein synthesis, blood vessel angiogenesis, and wound healing. It is a biological process involving reconstitution and the like (Steed, DL et al., Clin. Plast. Surg., 25:397 (1998)). When the skin is damaged, the proliferation of basal layer cells occurs actively in the epidermis.

Korean Patent Publication No. 10-1719223

Lactobacillus crustorum ( Lactobacillus crustorum ) strain is a strain found in sourdough, a fermented product used for bread dough, and nothing is known about its efficacy or skin improvement effect when ingested.

In one aspect, the present specification aims to provide a novel use of the Lactobacillus crustorum ( Lactobacillus crustorum ) strain.

In another aspect, the present specification aims to provide a Lactobacillus crustorum JTA strain having a skin condition improvement effect.

In one aspect, the technology disclosed herein is Lactobacillus crustorum ( Lactobacillus crustorum ) strain; lysate thereof; its culture; Or it provides a composition for improving skin condition comprising an extract of the strain, lysate or culture as an active ingredient.

In an exemplary embodiment, the strain may be Lactobacillus crustorum JTA whose accession number is KCCM12048P.

In an exemplary embodiment, the strain may have 16S rDNA comprising the nucleotide sequence of SEQ ID NO: 1.

In an exemplary embodiment, the extract may be a hot water extract.

In an exemplary embodiment, the active ingredient may be included in an amount of 0.001 to 30% by weight based on the total weight of the composition.

In an exemplary embodiment, the composition may be used for skin regeneration.

In an exemplary embodiment, the composition may have a skin texture improvement use.

In an exemplary embodiment, the composition may have a skin moisturizing application.

In an exemplary embodiment, the composition may be used for enhancing skin elasticity.

In an exemplary embodiment, the composition may have a skin barrier strengthening application.

In an exemplary embodiment, the composition may be used for accelerating ceramide synthesis.

In another aspect, the technology disclosed herein provides a Lactobacillus crustorum JTA strain having an accession number KCCM12048P.

In an exemplary embodiment, the strain may have a function to promote ceramide synthesis.

In an exemplary embodiment, the strain may have one or more skin condition improvement functions selected from the group consisting of skin regeneration, skin texture improvement, skin moisturizing, skin elasticity enhancement, and skin barrier strengthening.

In one aspect, the technology disclosed herein has an effect of providing a raw material of an eco-friendly material for improving skin condition.

In another aspect, the technology disclosed herein has an effect of providing the use of improving the skin condition of the Lactobacillus crustorum ( Lactobacillus crustorum ) strain. The strain provides skin regeneration, skin texture improvement, skin moisturizing, skin elasticity enhancement and skin barrier strengthening effect.

In another aspect, the technology disclosed herein has an effect of providing a Lactobacillus crustorum JTA strain having a skin condition improvement use.

Figure 1 shows the 16s rDNA sequence (SEQ ID NO: 1) of the Lactobacillus crustorum JTA strain whose accession number is KCCM12048P according to the present specification.
Figure 2 shows the skin cell growth promoting effect of the Lactobacillus crustorum strain according to an embodiment of the present specification (*: P<0.05 ).
Figure 3 shows the ceramide synthase activity promoting effect of the Lactobacillus crustorum strain according to an embodiment of the present specification (*: P<0.05 ).
Figure 4 shows the ceramide production enhancing effect of the Lactobacillus crustorum strain according to an embodiment of the present specification (*: P<0.05 ).

Hereinafter, the present invention will be described in detail.

In one aspect, the technology disclosed herein is Lactobacillus crustorum ( Lactobacillus crustorum ) strain; lysate thereof; its culture; Or it provides a composition for improving skin condition comprising an extract of the strain, lysate or culture as an active ingredient.

In another aspect, the technology disclosed herein provides a Lactobacillus crustorum JTA strain having an accession number KCCM12048P.

As used herein, the term "active ingredient" refers to a component capable of exhibiting the desired activity alone or in combination with a carrier having no activity by itself.

Unlike petroleum and water, microbial resources have the advantage of being used as a sustainable resource in that they can be reproduced.

In an exemplary embodiment, the strain is cultured and the culture solution is centrifuged, washed with sterilized physiological saline, dispersed in a solvent, such as sterile oil, and lyophilized to prepare a freeze-dried powder for use.

The lysate of the strain may mean a product obtained by crushing the strain itself by chemical or physical force.

The culture of the strain may mean a material containing some or all of the materials contained in the culture medium, regardless of the form of the culture, for example, a material containing a metabolite or secretion resulting from the culture of the strain. Or it may mean the lysate, the strain itself may be included in the culture. In addition, the culture may mean including a fermented product.

The extract may mean a product obtained by extracting the strain itself, the lysate of the strain, the culture of the strain, or a mixture thereof regardless of the extraction method, the extraction solvent, the extracted component, or the form of the extract, and after extraction, other methods It is a broad concept that includes all substances that can be obtained by processing or processing with

In an exemplary embodiment, the extract may be a heated extract.

In an exemplary embodiment, the extract may be extracted at a temperature of 50 °C or higher, 60 °C or higher, 70 °C or higher, 80 °C or higher, 90 °C or higher, or 100 °C or lower.

In an exemplary embodiment, the extract may be a hot water extract.

In an exemplary embodiment, the active ingredient may be included in an amount of 0.001 to 30% by weight based on the total weight of the composition. In another aspect, the active ingredient is 0.001 wt% or more, 0.01 wt% or more, 0.1 wt% or more, 0.5 wt% or more, 1 wt% or more, 1.5 wt% or more, or 2 wt% or more, based on the total weight of the composition, and 30 Weight % or less, 25 wt% or less, 20 wt% or less, 15 wt% or less, 10 wt% or less, or 5 wt% or less may be included.

The skin consists of the epidermal layer, the dermis layer, the subcutaneous fat layer, and the muscle layer. The stratum corneum of the epidermis layer itself is a cell membrane composed of a double structure of protein and lipid. As the protective barrier is reduced or the protective barrier is cracked, the double structure of the protein-lipid gradually loses its hydrophilic and lipophilic amphiphilic properties and falls off, thereby damaging the skin barrier. On the other hand, keratinocytes form the stratum corneum while generating intercellular lipids such as natural moisturizing factors and ceramides so that the stratum corneum has firmness and flexibility, elasticity and moisture, and functions as a reinforced skin barrier.

The composition according to the present specification promotes skin cell growth. In addition, the composition according to the present specification increases the amount of ceramide, which is a natural moisturizing factor and a component of the skin barrier, to enhance the degree of skin moisturizing and skin elasticity, thereby moisturizing the skin, improving skin texture, regenerating the skin, strengthening the skin barrier, and elasticity of the skin. It provides an excellent effect for strengthening.

The 'improving skin texture' may mean smoothing and softening the skin surface by suppressing or inhibiting roughening of the skin surface due to internal or external influences such as aging and stress.

The 'skin regeneration' refers to recovery of damaged skin or function of damaged skin by promoting skin cell growth, and the skin may refer to the epidermis, dermis, and subcutaneous tissue (subcutaneous fat).

In an exemplary embodiment, the strain may have 16S rDNA comprising the nucleotide sequence of SEQ ID NO: 1.

In an exemplary embodiment, the strain may have a function to promote ceramide synthesis.

In an exemplary embodiment, the strain may have one or more skin condition improvement functions selected from the group consisting of skin regeneration, skin texture improvement, skin moisturizing, skin elasticity enhancement, and skin barrier strengthening.

In another aspect, the technology disclosed herein provides a fermented product or an extract thereof using the strain as a fermentation strain.

In another aspect, the technology disclosed herein provides a composition for improving skin condition comprising a fermented product using the strain as a fermented strain or an extract thereof as an active ingredient.

In another aspect, the technology disclosed herein provides a method for producing a fermented food comprising the step of preparing a fermented food using the strain as a fermented strain.

In an exemplary embodiment, the composition may be a cosmetic composition.

In addition to the active ingredient, the cosmetic composition may further include functional additives and ingredients included in general cosmetic compositions. The functional additive may include a component selected from the group consisting of water-soluble vitamins, oil-soluble vitamins, high-molecular peptides, high-molecular polysaccharides, sphingolipids, and seaweed extract. Other ingredients included include oils and fats, moisturizers, emollients, surfactants, organic and inorganic pigments, organic powders, UV absorbers, preservatives, fungicides, antioxidants, plant extracts, pH adjusters, alcohols, pigments, fragrances, blood circulation An accelerator, a cooling agent, a limiting agent, purified water, etc. are mentioned.

The formulation of the cosmetic composition is not particularly limited, and may be appropriately selected according to the purpose. For example, powder, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nourishing lotion, massage cream, nourishing cream, moisture cream, hand cream, foundation, essence, nourishing essence, pack, shampoo , soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser may be prepared in any one or more formulations selected from the group consisting of, but is not limited thereto.

When the formulation of the present invention is a paste, cream or gel, animal fiber, vegetable fiber, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or a spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane /may contain propellants such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solvating agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol fatty esters, fatty acid esters of polyethylene glycol or sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, water, a liquid diluent such as ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol esters and polyoxyethylene sorbitan esters, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracanth may be used.

When the formulation of the present invention is a surfactant-containing cleansing agent, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide as carrier components Ether sulfate, alkylamidobetaine, aliphatic alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, linolin derivative or ethoxylated glycerol fatty acid ester and the like can be used.

When the formulation of the present invention is soap, alkali metal salts of fatty acids, fatty acid hemiester salts, fatty acid protein hydrolysates, isethionate, lanolin derivatives, aliphatic alcohols, vegetable oils, glycerol, sugar, etc. may be used as carrier components. have.

According to an exemplary embodiment, the composition may be a pharmaceutical composition.

In addition to the active ingredient, the pharmaceutical composition may further contain pharmaceutical adjuvants such as preservatives, stabilizers, wetting agents or emulsification accelerators, salts and/or buffers for osmotic pressure control, and other therapeutically useful substances, in a conventional method. It can be formulated in the form of various oral administration or parenteral administration according to the needs.

The oral administration agents include, for example, tablets, pills, hard and soft capsules, solutions, suspensions, emulsifiers, syrups, powders, powders, fine granules, granules, pellets, etc., and these formulations contain surfactants in addition to active ingredients. , diluents (e.g. lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and glycine), lubricants (e.g. silica, talc, stearic acid and its magnesium or calcium salts and polyethylene glycol) . Tablets may also contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and polyvinylpyrrolidine, optionally starch, agar, alginic acid or its sodium salt It may contain pharmaceutical additives such as disintegrants, absorbents, colorants, flavoring agents, and sweetening agents, such as. The tablet may be prepared by a conventional mixing, granulating or coating method.

In addition, the parenteral dosage form may be a transdermal dosage form, for example, injections, drops, ointments, lotions, gels, creams, sprays, suspensions, emulsions, suppositories, patches, etc. may be, but is not limited thereto.

Determination of the dosage of the active ingredient is within the level of those skilled in the art, and the daily dosage of the drug varies depending on various factors such as the progress of the subject to be administered, the onset time, age, health status, complications, etc. On the basis of, 1 μg/kg to 200 mg/kg of the composition in one aspect, and 50 μg/kg to 50 mg/kg in another aspect may be administered in divided doses 1 to 3 times a day, the dosage does not limit the scope of the present invention in any way.

The pharmaceutical composition may be an external preparation for the skin, and the external preparation for skin is a generic term that may include anything applied outside the skin, and pharmaceuticals of various formulations may be included here.

According to an exemplary embodiment, the composition may be a food composition.

The food composition may be in a liquid or solid form, for example, various foods, beverages, gum, tea, vitamin complexes, health supplements, etc., to be used in powder, granule, tablet, capsule or beverage form. can In addition to the active ingredient, the food composition of each formulation can be appropriately selected by those skilled in the art without difficulty depending on the formulation or purpose of use in addition to the active ingredient, and a synergistic effect may occur when applied simultaneously with other raw materials.

There is no particular limitation on the liquid ingredients that may be contained in addition to the active ingredients disclosed herein, and may include various flavoring agents or natural carbohydrates as additional ingredients, like conventional beverages. Examples of the natural carbohydrate include monosaccharides, disaccharides such as glucose and fructose, polysaccharides such as maltose and sucrose, conventional sugars such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol, and erythritol etc. As the above flavoring agents, natural flavoring agents (taumatin, stevia extract (eg, rebaudioside A, glycyrrhizin, etc.) and synthetic flavoring agents (eg, saccharin, aspartame, etc.) can be advantageously used. The proportion of the natural carbohydrate may be generally about 1 to 20 g, in one aspect, about 5 to 12 g per 100 ml of the composition disclosed herein.

In one aspect, the food composition includes various nutrients, vitamins, minerals (electrolytes), flavoring agents such as synthetic flavoring agents and natural flavoring agents, coloring agents and thickening agents (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohols, carbonation agents used in carbonated beverages, and the like. In another aspect, it may include pulp for the production of natural fruit juices and vegetable beverages. The above components may be used independently or in combination. The proportion of the additive may vary, but is generally selected in the range of 0.001 to about 20 parts by weight per 100 parts by weight of the composition disclosed herein.

Hereinafter, the present invention will be described in more detail through examples. These examples are only for illustrating the present invention, and it will be apparent to those of ordinary skill in the art that the scope of the present invention is not to be construed as being limited by these examples.

Example 1. Isolation and identification of strains

In the fermentation process of conventional wine (在來酒), Lactobacillus crustorum ( Lactobacillus crustorum ) strain was isolated and identified as follows.

By fermenting millet, Gangsul, a traditional liquor of Jeju Island, was made. Specifically, the tank was washed in running water, dried through hot air drying (60° C., 1 hour or more), and ground into powder. Knead the coarse powder to make a rice cake shape of about 5-6 cm, and cook it in a hot water bath. After mixing the yeast with 1/4 weight in the crude rice cake, it was transferred to a fermentation vessel and fermented at 17-20℃ for 2-3 months. Lactobacillus crustorum ( Lactobacillus crustorum ) was isolated from the fermented product collected within one month after the start of fermentation.

After diluting the fermented liquor with physiological saline (0.85% NaCl) at a ratio of 10 times and 100 times, an appropriate amount was dispensed on BCP agar medium (plate count agar with bromcresol purple), which is a lactic acid bacteria separation medium, at 30-37℃ 3 A single strain was finally isolated by culturing for one day, taking colonies that turned yellow and subcultured 2 to 4 times on MRS agar.

Identification of the isolated strain was carried out through 16S rRNA sequencing using primers 27F (5'-AGAGTTTGATCMTGGCTCAG-3', SEQ ID NO: 2) and 1492R (5'-TACGGYTACCTTGTTACGACTT-3', SEQ ID NO: 3). As a result of searching the analyzed nucleotide sequence in Gene Bank, the isolated strain was finally confirmed to have 99% similarity to Lactobacillus crustorum strain LMG 23700, named it Lactobacillus crustorum JTA, and deposited the strain on June 27, 2017 It was deposited with the institution, the Korea Center for Conservation of Microorganisms, and was given a deposit number KCCM12048P.

Example 2. Lactobacillus crustorum ( Lactobacillus crustorum ) Preparation of culture and extract of the strain

The Lactobacillus crustorum JTA strain identified in Example 1 was stationary cultured in MRS medium (MRS Broth) at 37° C. for 24 hours. When the bacterial concentration in the liquid medium reached 5 × 10 ⁸ ~ 1 × 10 ⁹ CFU/mL, the feeder cells were separated by centrifugation at 4000 rpm at 4°C, and the supernatant was filtered with a 0.45 μm filter to obtain a culture solution. .

In addition, the remaining cells were washed with PBS (phosphate buffered saline) and then ^{redispersed at a concentration of 5 × 10 8} to 1 × 10 ⁹ CFU/mL and heated at 100° C. for 10 minutes to prepare a heating eluate.

Example 3. Lactobacillus crustorum ( Lactobacillus crustorum ) Confirmation of the skin cell growth promoting effect of the strain

The effect of the Lactobacillus crustorum strain on skin cell growth was tested as follows.

HaCaT, a keratinocyte, was inoculated into a 96-well plate at a concentration ^{of 5×10 3} _{cells/well, and cultured for 1 day at 5% CO 2} , 37° C., followed by stabilization, and used in the experiment. The medium of keratinocytes was substituted with a serum-free medium (serum-free medium), and the culture solution of Lactobacillus crustorum prepared in Example 2 was treated by concentration. The untreated group was compared by adding the same amount of PBS (phosphate buffered saline). The experiment was repeated 3 times for each concentration. After 24 hours, the degree of cell proliferation was measured using MTT assay.

As a result of slowing the growth rate of skin cells with serum-free media and treating the culture medium of Lactobacillus crustorum, the proliferation of skin cells was promoted, and the culture medium of Lactobacillus crustorum isolated in the lecture was used for skin cells. It was confirmed that there is an effect of promoting growth (see FIG. 2).

Example 4. Lactobacillus crustorum ( Lactobacillus crustorum ) Confirmation of ceramide synthase activity promotion and ceramide production enhancement effect of the strain

The effect of the Lactobacillus crustorum strain on the activity of the ceramide synthase and the amount of ceramide produced was tested as follows.

HaCaT, a keratinocyte, ^{was prepared at a concentration of 2×10 5} cells/mL, inoculated 3 mL each in a 6 well plate, and _{cultured at 5% CO 2} , 37° C. for 1 day to stabilize and then used for the experiment. The culture medium of Lactobacillus crustorum prepared in Example 2 was treated by concentration in the keratinocyte medium, and the same amount of phosphate buffered saline (PBS) was added to the untreated group for comparison. The experiment was repeated 3 times for each concentration.

After 24 hours, the medium for keratinocytes was removed, and the cells were rinsed twice with 3 mL of PBS to completely remove the medium. The keratinocytes were treated with 300 μL of lysis buffer per well to collect the cells and their eluates, and centrifuged to collect only the eluates. The amount of protein in the eluate of each well was corrected to be the same, and the activity of the ceramide synthase was measured using an acidic sphingomyelinase assay kit (abcam, #ab190554). As a result, it was confirmed that the skin cells were treated with the culture solution of Lactobacillus crustorum isolated in Gangsul to increase the activity of the ceramide synthase (see FIG. 3 ).

In addition, in the same manner as above, the keratinocytes were treated with the culture solution of Lactobacillus crustorum prepared in Example 2, and an eluate of the keratinocytes was prepared. The amount of protein in the centrifuged eluate was adjusted to the same level and lipids were extracted using ethanol. The amount of ceramide was measured by HPLC after derivatizing the lipid extract with ο-phthalaldehyde. As a result, based on the amount of ceramide in the untreated group, it was confirmed that the keratinocytes treated with the culture solution of Lactobacillus crustorum increased the amount of ceramide production (see FIG. 4 ).

Formulation examples of the composition according to one aspect of the present specification are described below, but other various formulations are also applicable, which is not intended to limit the present invention, but merely to describe it in detail.

Formulation Example 1. Softening lotion

0.01% by weight of the Lactobacillus crustorum culture solution of Example 2, 3% by weight of glycerin, 2% by weight of butylene glycol, 2% by weight of propylene glycol, 0.1% by weight of carboxyvinyl polymer, 10% by weight of ethanol, 0.1% by weight of triethanolamine And the remaining amount of preservatives, trace dyes, trace fragrances and trace purified water were mixed to prepare a softening lotion according to a conventional method.

Formulation Example 2. Nutrient lotion

0.01% by weight of the Lactobacillus crustorum culture medium of Example 2, 4% by weight of beeswax, 1.5% by weight of polysorbate 60, 0.5% by weight of sorbitan sesquioleate, 5% by weight of liquid paraffin, 5% by weight of squalane, capryl Ric/capric triglyceride 5% by weight, glycerin 3% by weight, butylene glycol 3% by weight, propylene glycol 3% by weight, carboxyvinyl polymer 0.1% by weight, triethanolamine 0.2% by weight, balance of preservative, trace colorant, trace fragrance And a small amount of purified water was mixed and prepared according to a conventional nutrient lotion preparation method.

Formulation Example 3. Nourishing Cream

0.01% by weight of the Lactobacillus crustorum culture medium of Example 2, 10% by weight of beeswax, 1.5% by weight of polysorbate 60, 0.5% by weight of sorbitan sesquioleate, 10% by weight of liquid paraffin, 5% by weight of squalane, capryl Ric/capric triglyceride 5% by weight, glycerin 5% by weight, butylene glycol 3% by weight, propylene glycol 3% by weight, triethanolamine 0.2% by weight and the remaining amount of preservatives, trace colors, trace fragrances and trace purified water are usually mixed It was prepared according to the nutrition cream manufacturing method of

Formulation Example 4. Pack

0.01% by weight of the Lactobacillus crustorum culture medium of Example 2, 13% by weight of polyvinyl alcohol, 0.2% by weight of sodium carboxymethylcellulose, 0.1% by weight of allantoin, 5% by weight of ethanol, 0.3% by weight of nonylphenyl ether, and the remaining amount of preservative , a trace dye, a trace fragrance, and a trace purified water were mixed and prepared according to a conventional pack manufacturing method.

Above, a specific part of the present invention has been described in detail, for those of ordinary skill in the art, this specific description is only a preferred embodiment, and the scope of the present invention is not limited thereby. It will be obvious. Accordingly, it is intended that the substantial scope of the present invention be defined by the appended claims and their equivalents.

Korea Microorganism Conservation Center KCCM12048P 20170627

<110> AMOREPACIFIC CORPORATION <120> COMPOSITION COMPRISING CULTURE OR ITS EXTRACT OF LACTOBACILLUS CRUSTORUM <130> 17P426/IND <160> 3 <170> KoPatentIn 3.0 <210> 1 <211> 883 <212> DNA <213> Unknown <220> <223> Lactobacillus crustorum JTA <400> 1 cccagcggag tgcttaatgc gttagctgca gcactgaagg gcggaaaccc tccaacactt 60 agcactcatc gtttacggca tggactacca gggtatctaa tcctgtttgc tacccatgct 120 ttcgaatctc agcgtcagtt acagaccaga aagccgcctt cgccactggt gttcttccat 180 atatctacgc attccaccgc tacacatgga gttccacttt cctcttctgc actcaagttt 240 accagttttc gaagcacttc ctcggttgag ccgagggctt tcacttcaaa cttaataaac 300 cgcctacatt ctctttacgc ccaataaatc cggacaacgc ttgccaccta cgtattaccg 360 cggctgctgg cacgtagtta gccgtggctt tctggttgaa taccgtcagt acgtgaacag 420 ttactctcac gcatgttctt cttcaacaac agagttttac gatccgaaaa ccttcttcac 480 tcacgcggca ttgctccatc aggctttcgc ccattgtgga agattcccta ctgctgcctc 540 ccgtaggagt ttgggccgtg tctcagtccc aatgtggccg attaccctct caggtcggct 600 acgtatcatt gccttggtga gctattacct caccaactag ctaatacgcc gcgggtccat 660 ccaaaagcga tagcagagcc atctttcaag ctacgatcat gagaaagtag ttgttatgcg 720 gtattagcac ctgtttccag atgttatccc ccacttttgg gcaggttacc cacgtgttac 780 tcacccgtcc gccactcacc aaggtctgaa tcatgaagca agcttcaatc ttcaggatgg 840 ttcgttcgac ttgcatgtat taggcatgcc gccagcgttc gtc 883 <210> 2 <211> 20 <212> DNA <213> Artificial Sequence <220> <223> 27F primer <400> 2 agagtttgat cmtggctcag 20 <210> 3 <211> 22 <212> DNA <213> Artificial Sequence <220> <223> 1492R primer <400> 3 tacggytacc ttgttacgac tt 22

Claims (14)

Lactobacillus crustorum ( Lactobacillus crustorum ) strain; lysate thereof; its culture; Or a composition for improving skin condition comprising an extract of the strain, lysate or culture as an active ingredient.
The method of claim 1,
The strain is Lactobacillus crustorum JTA with an accession number of KCCM12048P, a composition for improving skin condition.
3. The method of claim 2,
The strain is to have a 16S rDNA comprising the nucleotide sequence of SEQ ID NO: 1, a composition for improving skin condition.
The method of claim 1,
The extract is a hot water extract, a composition for improving skin condition.
The method of claim 1,
The active ingredient is included in an amount of 0.001 to 30% by weight based on the total weight of the composition, a composition for improving skin condition.
The method of claim 1,
The composition has a skin regeneration use, a composition for improving skin condition.
The method of claim 1,
The composition has a skin texture improvement use, a composition for improving skin conditions.
The method of claim 1,
The composition has a skin moisturizing use, a composition for improving skin condition.
The method of claim 1,
The composition has a skin elasticity strengthening use, a composition for improving skin condition.
The method of claim 1,
The composition has a skin barrier strengthening use, a composition for improving skin conditions.
The method of claim 1,
The composition has a ceramide synthesis promoting use, a composition for improving skin condition.
Accession number KCCM12048P Lactobacillus crustorum ( Lactobacillus crustorum ) JTA strain.
13. The method of claim 12,
The strain will have a ceramide synthesis promoting function, the strain.
13. The method of claim 12,
The strain will have one or more skin condition improvement functions selected from the group consisting of skin regeneration, skin texture improvement, skin moisturizing, skin elasticity enhancement and skin barrier strengthening.

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